Microprism-based two-photon imaging of the mouse inferior colliculus reveals novel organizational principles of the auditory midbrain
- Department of Molecular and Integrative Physiology, University of Illinois at Urbana Champaign, United States
- Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, United States
- School of Molecular & Cell Biology, University of Illinois at Urbana-Champaign, United States
- Neuroscience Program, University of Illinois at Urbana-Champaign, United States
- Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, United States
Figures
Figure 1
A brief illustration of the surgical procedures.
(A) A surface view of the mouse’s skull showing the landmarks of the craniotomy over the IC. (B) An image of the bone flap after finishing the drilling. (C–E) A cartoon and two images of the brain …
Figure 2
The distribution of GABAergic cells on the LC(microprism) and DC imaged from the dorsal surface.
All images were taken for the GFP signals exclusively expressed in GABAergic cells. (A, B) Low magnification of fluorescence images for the GFP signals obtained from the IC surface with or without …
Figure 3 with 1 supplement
Two-photon (2P) expression of jRGECO1a along with evoked signals in the DC imaged from the dorsal surface and LC(microprism).
(A, B) The 2P images of the jRGECO1a expression on the DC imaged from the dorsal surface and the LC(microprism), respectively. (C, D) The time traces of the evoked calcium signals by either …
Figure 3—figure supplement 1
The expression of jRGECO1a in the IC across different regions and cell types.
(A) Image of the inferior colliculus (IC) showing the immunostaining to NueN (blue-column 1), GAD67-GFP (green-column 3), jRGECO1a (red-column 3), merged image for the three proteins (column 4), and …
Figure 4 with 1 supplement
The auditory cellular organization of the LC(microprism) vs dorsal cortex (DC) imaged from the dorsal surface.
(A, C) The two-photon (2P) images of GFP (green) and jRGECO1a expression (red) on either the LC(microprism) or the DC imaged from the dorsal surface, respectively. (B, D) The pseudocolor images show …
Figure 4—figure supplement 1
The best regression fit between best tone frequency and different anatomical axes.
(A, B) Linear and nonlinear quadratic regression fit between the best pure tone frequencies (BTFs) and the locations of the cells of the LC(microprism) and the dorsal cortex (DC), respectively.
Figure 5
The cellular response to pure tones and AM-noise of the LC(microprism) vs DC imaged from the dorsal surface.
(A) The cumulative distribution function of the best tone frequencies of all cells collected either from the LC imaged via microprism (blue line) or the DC imaged directly from its dorsal surface …
Figure 6
The waveforms of the somatosensory vs acoustic responses of the LC(microprism) and the dorsal cortex (DC) imaged via microprism.
Examples of different cell types based on their responses to auditory or somatosensory stimulations as well as their response timing to the auditory stimulation as indicated by waveforms of the …
Figure 7
Types of somatosensory and auditory responses from the lateral cortex (LC) neurons imaged via microprism.
(A) The two-photon (2P) images of GFP (green) and jRGECO1a expression (magenta) in the LC(microprism) show the modules within white irregular lines. (B, D, F) The pseudocolor images show the …
Figure 8
The somatosensory vs auditory responses over the dorsal cortex (DC) neurons.
(A) A low magnification of the image obtained from the inferior colliculus (IC) surface. (B–D) The top row: the two-photon (2P) images of GFP (green) and jRGECO1a expression (magenta) of three main …
Figure 9 with 1 supplement
The acoustic response from LC(microprism) and dorsal cortex (DC) imaged from the dorsal surface in awake animals.
(A, C) The two-photon (2P) images of GFP (green) and jRGECO1a expression (magenta) on either the LC(microprism) surface imaged via the microprism or the DC imaged directly from the dorsal surface, …
Figure 9—figure supplement 1
The best regression fit between best tone freuency and different anatomical axes.
(A,B) Linear and nonlinear quadratic regression fit between the best pure tone frequencies (BTFs) and the locations of the cells of the LC(microprism) and the dorsal cortex (DC), respectively.
Figure 10 with 1 supplement
The cellular response to pure tones and AM-noise of the LC(microprism) vs dorsal cortex (DC) imaged from the dorsal surface in awake animals.
(A) The cumulative distribution function of the best tone frequencies of all cells collected either from the lateral cortex (LC) imaged via microprism (blue line) or the DC imaged directly from its …
Figure 10—figure supplement 1
The receptive field sum (RFS) of dorsal cortex (DC) and lateral cortex (LC) neurons at different experimental preparations.
The cumulative distribution function of the RFS of all cells collected from DC or LC under different experimental preparations; Kruskal-Wallis ANOVA, χ2=346.9, p<0.001, post hoc Dunn’s test; *(DC vs …
Figure 11 with 1 supplement
The somatosensory responses from LC(microprism) in awake animals.
(A, C, E) The pseudocolor images show the auditory (white circles) or somatosensory (blue circles) responsive cells of the LC(microprism) as well as the merged response (green circles), …
Figure 11—figure supplement 1
The activity of the nonresponsive cells of the LC(microprism) in awake animals.
(A) The pseudocolor image shows the nonresponsive (red circles) vs responsive (white circles) cells to the auditory stimulation with unmodulated broadband noise at the LC(microprism). (B) The …
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | GAD67-GFP (GAD1GFP) | Generously given | N/A | Developed and shared with permission from Dr. Yuchio Yanagawa at Gunma University and obtained from Dr. Douglas Oliver at the University of Connecticut |
| Strain, strain background (Mus musculus) | Tg(Thy1-jRGECO1a)GP8.20Dkim/J | Jackson Laboratory, USA | 30525 | |
| Sequence-based reagent | jRGECO1a – primer | Transnetyx, USA | mApple-1 Tg | |
| Antibody | NeuN (D4G40) XP Rabbit mAB | Cell Signaling, USA | 62994 | (1:100) |
| Antibody | Goat anti-rabbit IgG conjugated to Alexa Fluor 405 | Thermo Fisher, USA | A-31556 | (1:200) |
