Rapid bacterial evaluation beyond the colony forming unit in osteomyelitis
- Centre for Orthopaedic & Trauma Research, Faculty of Health and Medical Sciences, University of Adelaide, Australia
- Department of Orthopaedics and Trauma, Royal Adelaide Hospital, Australia
Figures
Figure 1
Validation of DNA preparations from SaOS2-OY cells and S. aureus.
Digital droplet PCR (ddPCR) genome counting of SaOS2-OY (A), SK2 (B), and SK3 (C), comparing the Direct buffer approach (
) and a standard DNA kit (
) (four biological replicates with mean and …
Figure 2
Measurement of intracellular S. aureus in SaOS2-OY cells in vitro.
Colony-forming unit (CFU) recovery of SK2 (A) and SK3 (B) from host SaOS2-OY cells, with haemolysis reactions (
) and numerous small colony variants (
) shown in SK2 group; quantification of SK2 …
Figure 3 with 2 supplements
Pathogen diagnosis in clinical PJI bone specimens.
Masson’s trichrome staining of bone tissue sections of an osteoarthritis (OA) subject (A) and culture-negative periprosthetic joint infection (PJI) subjects I–III (B–D). Pathogen profiling using …
Figure 3—figure supplement 1
Polymerase chain reaction (PCR) analysis of bone samples from primary total hip replacement cases.
DNA isolated from five patient bone samples were analysed by PCR for the presence of human COL10A1 and bacterial tuf. The negative presence of bacterial tuf PCR product was confirmed by melt …
Figure 3—figure supplement 2
Multi-sequence alignment among S. aureus, S. epidermidis, S. haemolyticus, and S. hominis.
The green and blue boxes indicate the forward and reverse primer-binding areas, respectively; the region between the red brackets was the assaying sequence for analysis (A). A summary of the number …
