A scale-invariant log-normal droplet size distribution below the critical concentration for protein phase separation
- Department of Physics and Astronomy, University of Padova, Italy
- Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, United Kingdom
- National Institute for Nuclear Physics (INFN), Padova Section, Italy
- Department of Biomedical Sciences, University of Padova, Italy
Figures
Figure 1
Determination of the critical exponents for FUS of the scaling invariance.
Determination of the exponent φ for FUS (A) and SNAP-tagged FUS (C). The ratios of the average moments of the droplet sizes (<sk+1>/<sk > , at k = 0.5, 1, 1.5, 2, Equation 4) are represented at …
Figure 2
Log-normal behaviour of FUS and SNAP-tagged FUS size distributions below the critical concentration.
Variation of the size distribution with protein concentration: ln s0 (A) and σ (B). lns0 and σ (inset) were computed for FUS (blue) and SNAP-tagged FUS (red) using Equation 11. Error bars (in inset …
Figure 3
Estimation of the critical concentration of FUS using the scale invariance.
Critical concentration of FUS (5.0 ± 0.2 μM) (A) and SNAP-tagged FUS (5.4 ± 0.4 μM) (B). The scaling model predicts that the function of the moments plotted versus the concentration ρ becomes a …
Figure 4
Collapse of the droplet size distributions of FUS as predicted by the scale invariance.
If the scaling ansatz of Equation 2 holds, the standard deviation σ of the log-normal distribution should not depend on the distance from the critical concentration, and a collapse should be …
Figure 5
Estimation of the critical concentration of α-synuclein using the scale invariance.
(A) Determination of the critical exponent φ. The ratios of the average moments of the droplet sizes (<sk+1>/<sk>, at k = 0.25, 0.75, 1.25, 1.75, Equation 4) are represented at various distances …
Figure 6
The droplet size distribution is stationary below the critical concentration.
(A) Images of α-synuclein droplets at increasing concentrations of α-synuclein. (B, C) After an initial transient of 5 min, the droplet size distributions remain approximately stationary below the …
Additional files
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Supplementary file 1
Table S1.
Size distributions of FUS and SNAP-FUS condensates below their critical concentrations.
- https://cdn.elifesciences.org/articles/94214/elife-94214-supp1-v1.xlsx
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Supplementary file 2
Table S2.
Size distributions of α-synuclein condensates below their critical concentration.
- https://cdn.elifesciences.org/articles/94214/elife-94214-supp2-v1.xlsx
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Supplementary file 3
Table S.
Size distributions of α-synuclein condensates at 50 μM concentration as a function of the time.
- https://cdn.elifesciences.org/articles/94214/elife-94214-supp3-v1.xlsx
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MDAR checklist
- https://cdn.elifesciences.org/articles/94214/elife-94214-mdarchecklist1-v1.docx
