(a) Growth curves of E. faecium WT, ΔsagA, and complementation strains with functional and nonfunctional sagA genes (n=3). Data are presented as mean value ± standard deviation. (b) ɑ-SagA western …
Excel file containing numeric data used to generate Figure 1a.
Uncropped western blots and gels (total protein) used to generate Figure 1b.
Uncropped images used to generate Figure 1c.
Uncropped images used to generate Figure 1d.
Validation of sagA deletion and image of bacterial strain growth.
Uncropped images used to generate Figure 1d.
(a) PCR validation of ΔsagA deletion. (b) ΔsagA cells are sedimented at the bottom of the tube compared to E. faecium wild-type (WT) and ΔsagA / psagA.
Uncropped gel used to generate Figure 1—figure supplement 1a and uncropped photo used to generate Figure 1—figure supplement 1b.
(a) Approximate minimum inhibitory concentration (MIC) determinations via antibiotic test strips for E. faecium wild-type (WT), ΔsagA and ΔsagA/ psagA. White arrows point to MIC values (summary of …
Uncropped images used to generate Figure 1—figure supplement 2a.
Excel file containing numeric data used to generate Figure 1—figure supplement 2b.
(a-c) Representative tomographic slices (n=3) of E. faecium strains: E. faecium wild-type (WT) (a), ΔsagA (b), and ΔsagA/psagA (c). Cell division septa are indicated by white arrows. Scale bar = 100 …
Excel file containing numeric data to generate Figure 2e–f.
(a) Representative cryo-electron tomography (cryo-ET) images of E. faecium wild-type (WT), ΔsagA, and ΔsagA/psagA are shown in the top row. The cell wall, septum, divisome, and ribosome are …
Excel file containing numeric data to generate Figure 2—figure supplement 1c–e.
(a) Cartoon model depicting cell division site in the xy coordinate plane (left) and xz coordinate plane (right). The two boxes shown in right represent the cryo-focused ion beam (cryo-FIB) milling …
Excel file containing numeric data used to generate Figure 2—figure supplement 2f–g.
(a) Relative abundance of muropeptides isolated from mutanolysin-digested sacculi of E. faecium strains and analyzed by LC-MS (n=6). Green asterisks highlight changes in abundance of small …
Excel file containing numeric data used to generate Figure 3a and c–e.
(a) Representative LC-MS chromatograms of mutanolysin-digested peptidoglycan isolated from sacculi of E. faecium wild-type (W)T (top), ΔsagA (middle), and ΔsagA/ psagA (bottom). Numbers correspond …
Excel file containing numeric data used to generate Figure 3—figure supplement 1a.
(a) Schematic of tumor growth experiment: mice were provided water containing antibiotics for one week and started drinking bacteria three days before tumor implantation. Once the tumor reaches ~100 …
Excel file containing numeric data used to generate Figure 4b–j.
We first identified total tumor infiltrating cells by forward and side scatter gating. We then selected single cells using forward scatter area (FSC-A) versus forward scatter height (FSC-H) …
Excel file containing numeric data used to generate Figure 4—figure supplement 1.
Mutations detected in ΔsagA E. faecium Com15 strain.
Summary of MIC determinations via antibiotic test strips for E. faecium wild-type (WT), ΔsagA, and ΔsagA/psagA (Figure 1—figure supplement 2a).
E. faecium strains used in this study.
Plasmids used in this study.
Primers used in this study for generating complementation plasmids and empty vector.
Primers used in this study for SagA mutagenesis.
Masses of peptidoglycan fragments were detected with MSD API-ES.