(A and B) show representative (unattached) prototype coils in the single loop and ‘figure 8’ styles, respectively. (C) Box-and-whisker plot presents the cortical-specific signal-to-noise ratio (SNR) …
C1 and C4 are 2.2 pF capacitors. C2 is a 0.6–2.5 pF trimmer. C3 is a 5–18 pF trimmer.
(A) shows representative (unimplanted) coils in the single loop (left) and ‘figure 8’ styles (right). Table 2 provides a parts list and cost for making these coils and Figure 1—figure supplement 1 …
(A). The awake mouse setup with head-fixed position in a custom-built cradle for visual and vibrissa stimulation. (B) The representative fMRI time course of an awake mouse based on raw image data …
(A) Box-and-whisker plot showing eye movements over 10 min duration of training days showing an increase in eye movements over the training duration indicating the animals are becoming more relaxed …
Struggling-induced spikes are already substantially minimized following the training with only 5 volumes (2%) censored. After 1 month of scanning there was only one single repetition (repetition …
(A) The brain-wide functional maps of awake mice show strong positive blood oxygen level-dependent (BOLD) activation in the visual cortex (VC), lateral geniculate nucleus (LGN), superior colliculus …
Each ‘TR period’ is 2 s. The stimulation is repeated 10 times over the 410 s acquisition period which includes a 10 s baseline acquisition period before the first stimulation. This paradigm results …
The workflow diagram is described through the following steps: raw data registration, outlier removal, volume registration-based motion file estimation, motion removal, linear regression with the …
(Left) BOLD activation map highlighting positive activation in the barrel cortex (BC) and selection of a 3×3 voxel display of time course data (green box). (Right) Time course data taken from 3×3 …
(A) The brain-wide functional maps of awake mice show the strong positive blood oxygen level-dependent (BOLD) activation in the contralateral barrel cortex (BC) and ventral posteromedial nucleus …
(A) The VRA-based correlation maps at –6 s and 0 s time shifts of awake mice with repetitive stimulation (REP). The strong correlation in the contralateral barrel cortex (BC) is shown in the …
(A) shows representative coils in the single loop (left) and ‘figure 8’ styles (right). Supplementary Table 1 provides a parts list and cost for making these coils and Supplementary Figure 1 …
Images are of connectivity map projections from the Allen Brain Atlas derived from a Cre-dependent AAV tracing of axonal projections (Oh et al., 2014)
The real-time tracer from the selected point demonstrates the time points with motion, as well as the motion-induced image distortion during awake mouse fMRI.
rs → resting-state fMRI, stim → whisker stimulation fMRI.
Training day of phase | Phase 1(hold in hand) | Phase 2(holder+pupil) | Phase 3(mock-MRI+pupil) | Phase 4(EPI+pupil) |
---|---|---|---|---|
1 | 5 min | 15 min | 30 min | 60 min (rs) |
2 | 10 min | 30 min | 30 min | 60 min (rs) |
3 | 30 min | 60 min (stim) | ||
4 | 30 min | 60 min (stim) | ||
5 | 60 min | |||
6 | 60 min | |||
7 | 60 min | |||
8 | 60 min |
Part description | Cost |
---|---|
FR-4 PCB Chip, 0.4×0.8 in, 0.062 in thick | $214.00/200 |
Capacitor 2.2 pF 2KV | $309.80/400 |
Trimmer 0.6–2.5 pF | $1649.28/200 |
Trimmer 5–18 pF | $1640.56/200 |
Magnet wire 20AWG | $118.28/spool/710.8’ |
MCX socket | $1027.14/200 |
Entire coil built by MRIBOT | $300/each |
Training Day of Each Phase | Phase 1 (hold in hand) | Phase 2 (holder +pupil) | Phase 3 (Mock-MRI+pupil) | Phase 4 (EPI +pupil) |
---|---|---|---|---|
1 | 5 mins | 15 mins | 30 mins | 60 mins (rs) |
2 | 10 mins | 30 mins | 30 mins | 60 mins (rs) |
3 | 30 mins | 60 mins (stim) | ||
4 | 30 mins | 60 mins (stim) | ||
5 | 60 mins | |||
6 | 60 mins | |||
7 | 60 mins | |||
8 | 60 mins |