Neutron spectrometers with different resolutions (1, 13, 25.4, and 100 μeV) were applied. Elatic intensity S(q, Δt) of (a, b) dry H-LYS and H-LYS in D2O at h = 0.3, (c, d) dry H-MYO and H-MYO in D2O …
Structures of (a) lysozyme (LYS), (b) myoglobin (MYO), (c) cytochrome P450 (CYP), and (d) green fluorescent protein (GFP).
Mean-squared atomic displacements <x2(Δt)> derived from Figure 1 using Gaussian approximation, of (a, b) dry H-LYS and H-LYS in D2O at h = 0.3, (c, d) dry H-MYO and H-MYO in D2O at h = 0.3, and (e–g)…
S(q, Δt) of (a) dry H-CYP and H-CYP in D2O at h = 0.2 and 0.4 and (c) dry H-LYS and H-LYS in D2O at h = 0.18, 0.30, and 0.45, all measured using HFBS with the instrumental resolution of 1 μeV. All …
, derived from Figure 2c using Gaussian approximation, of dry H-CYP and H-CYP in D2O at h = 0.2 and 0.4.
Neutron spectrometers with different resolutions (1, 25.4, and 100 μeV) were applied. S(q, Δt) of (a, b) dry D-GFP and D-GFP in H2O at h = 0.4, and (c–e) dry D-CYP and D-CYP in H2O at h = 0.4.
Mean-squared atomic displacements <x2(Δt)>, derived from Figure 3 using Gaussian approximation, of (a, b) dry D-GFP and D-GFP in H2O at h = 0.4, (c–e) dry D-CYP and D-CYP in H2O at h = 0.4.
S(q, Δt), for dry D-CYP and D-CYP in H2O at h = 0.2 and 0.4, measured using HFBS neutron instrument with an energy resolution of 1 μeV.
<x2(Δt)>, derived from Figure 4 using Gaussian approximation, of dry D-CYP and D-CYP in H2O at h = 0.2 and 0.4.
Alpha-helix | Beta-sheet | Loop and turn | |
---|---|---|---|
CYP (h = 0.2) | 52% | 11% | 37% |
CYP (h = 0.4) | 52% | 11% | 37% |
1 ns | 80 ps | 40 ps | 10 ps | |
---|---|---|---|---|
LYS | 213 K | 213 K | - | - |
MYO | 198 K | 198 K | - | - |
CYP | 228 K | - | 228 K | 228 K |
1 ns | 80 ps | 40 ps | 10 ps | |
---|---|---|---|---|
LYS | 212 K | 213 K | - | - |
MYO | 197 K | 199 K | - | - |
CYP | 228 K | - | 227 K | 228 K |
1 ns | 80 ps | 40 ps | 10 ps | |
---|---|---|---|---|
LYS (h = 0.3) | 213 K | 213 K | - | - |
MYO (h = 0.3) | 198 K | 198 K | - | - |
CYP (h = 0.4) | 228 K | - | 228 K | 228 K |
0.18 | 0.2 | 0.3 | 0.4 | 0.45 | |
---|---|---|---|---|---|
LYS (1 ns) | 225 K | - | 213 K | - | 195 K |
CYP (1 ns) | - | 248 K | - | 228 K | - |
CYP (TDSC) | - | 245 K | - | 225 K | - |
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Escherichia coli) | Escherichia coli, BL21(DE3) | Sigma-Aldrich | CMC0016 | |
Peptide, Recombinant protein | Lysozyme, chicken egg white | Sigma-Aldrich | CAS: 12650-88-3 | |
Peptide, Recombinant protein | Myoglobin, equine skeletal muscle | Sigma-Aldrich | CAS: 100684-32-0 | |
Chemical compound, drug | H2O | Millipore | ||
Chemical compound, drug | D2O | Sigma-Aldrich | CAS: 7789-20-0 |