A new potential strategy for cutaneous squamous cell carcinoma treatment by generating serum-based antibodies from tumor-exposed mice
- College of Medical Laboratory Science, Guilin Medical University, China
Figures
Figure 1
The workflow of this study.
Figure 2
The serum-based antibodies treatment reduces tumor volume.
(A) Tumor growth induced by Dimethylbenz(a)anthracene (DMBA)/Tetradecanoylphorbol-13-acetate (TPA) and changes in tumor volume before and after serum treatment. At week 12, the tumor volume reached its peak. Week 13 was dedicated to the isolation and expansion of the tumor cell. In week 14, the tumor cells were injected into the tail vein of paired mice in the serum provider group to produce serum-based antibodies. Weeks 15, 16, and 17 represent mice in tumor + serum treatment group receiving three times of serum treatment, respectively. Results are presented as the mean ± standard deviation (SD) obtained from at least three biological replicates. A paired two-tailed t-test was used for statistical evaluation. Asterisks indicate the following: **p<0.01. (B) Tumor cells isolated and cultured from mouse cutaneous squamous cell carcinoma (mCSCC). Scale bar, 100 µm.
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Figure 2—source data 1
Excel file of mouse weight and tumor volume data is shown in Figure 2.
- https://cdn.elifesciences.org/articles/95678/elife-95678-fig2-data1-v1.xlsx
Figure 3
The serum-based antibodies treatment reverses the expression of p53, Bcl-xL, NF-κB, and Bax.
ELISA analysis revealed the changes in the expression of p53, Bcl-xL, NF-κB, and Bax proteins before and after serum treatment. The tumor volume reached its peak at week 12. The mice in the tumor + serum treatment group received serum treatment at weeks 15, 16, and 17, respectively. Results are presented as the mean ± standard deviation (SD) obtained from at least three biological replicates. Statistical significance was determined by a paired two-tailed t-test. Asterisks indicate the following: **p<0.01.
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Figure 3—source data 1
Excel file of ELISA analysis data for p53, Bcl-xL, NF-κB, and Bax expression is shown in Figure 3.
- https://cdn.elifesciences.org/articles/95678/elife-95678-fig3-data1-v1.xlsx
Figure 4
Schematic diagram of experimental design.
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A new potential strategy for cutaneous squamous cell carcinoma treatment by generating serum-based antibodies from tumor-exposed mice
eLife 13:RP95678.
https://doi.org/10.7554/eLife.95678.3
