Attentional modulation of secondary somatosensory and visual thalamus of mice
- Department of Neuroscience, Columbia University, United States
- Department of Physiology, Anatomy, & Genetics, University of Oxford, United Kingdom
Figures
Figure 1 with 1 supplement
Mice can be conditioned to attend to one sensory modality while ignoring a second modality.
(a) Behavioral setup. Mice were head-fixed and presented with a visual stimulus (a drifting grating on a screen) or a tactile stimulus (an air puff to the distal portion of the whiskers). (b) …
Figure 1—figure supplement 1
Latency of anticipatory licking does not depend on the modality of the conditioned stimulus.
(a) Latency to the first lick that occurred after the presentation of the air puff in tactilely conditioned mice on the day of recording. Box plots depict the distribution of lick latencies of …
Figure 2 with 1 supplement
Array recordings were made simultaneously from posterior medial nucleus (POm) and lateral posterior nucleus (LP).
(a) Example coronal section. Red: DiI marking recording track. Gray: endogenous biotin stained with streptavidin Alexa fluor 647. (b) Zoom of the outlined region in a. The borders of LP, POm, and …
Figure 2—figure supplement 1
Manual curation of posterior medial nucleus (POm) and lateral posterior nucleus (LP) cells.
(a, b) Example POm cells. Left, Mean spike waveform (black) with 200 randomly sampled individual waveforms (gray). Right, Autocorrelogram, binned at 0.5 ms. Gray lines indicate a time lag of ± 1 ms. …
Figure 3
Posterior medial nucleus (POm) responses to tactile and visual stimuli depend on the conditioning paradigm.
(a) Example cells from a tactilely conditioned mouse (same mouse as in Figure 1g, session 7). Top row: spike raster aligned to the onset of the drifting grating (blue). Middle row: spike raster …
Figure 4
Conditioning alters tactile stimulus response latencies in posterior medial nucleus (POm).
(a) Event-triggered average firing rates of all POm cells (mean ± SEM) aligned to either the air puff (red) or the drifting grating (blue) in tactilely conditioned mice (left) and visually …
Figure 5
Posterior medial nucleus (POm) correlates with arousal and movement regardless of conditioning.
(a) Pupil and whisker tracking. Top left: Example frame from an eye video with the edge of the pupil highlighted in green. Top Right: A sample trace of pupil radius over time (gray: raw; green: …
Figure 6
Posterior medial nucleus (POm) responses to tactile and visual stimuli vary based on anatomical location.
(a) Sensory selectivity index for significantly responding POm cells (colored cells in 3d and 3f). Cells with a positive selectivity index more strongly respond to the air puff, and cells with a …
Figure 7 with 2 supplements
Conditioning also reshapes lateral posterior nucleus (LP) responses.
(a) Baseline firing rates of all posterior medial nucleus (POm) and LP cells separated by conditioning type. POm cells had higher firing rates than LP cells, and firing rates did not significantly …
Figure 7—figure supplement 1
Conditioning alters stimulus response latencies in lateral posterior nucleus (LP).
(a) Event-triggered average firing rates of all LP cells (mean ± SEM) aligned to either the air puff (red) or the drifting grating (blue) in tactilely conditioned mice (left) and visually …
Figure 7—figure supplement 2
Lateral posterior nucleus (LP) responses to tactile and visual stimuli as a function of anatomical location.
(a) Stimulus selectivity index for significantly responding LP cells. Cells with a positive selectivity index primarily respond to the air puff and cells with a negative index primarily respond to …
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Software, algorithms | MATLAB | MathWorks, Inc. | v2022b | |
| Software, algorithms | Kilosort | https://github.com/MouseLand/Kilosort (Pennington et al., 2024) | v3 | |
| Software, algorithms | Phy | https://github.com/cortex-lab/phy | v2.01b | |
| Software, algorithms | Arduino IDE | https://www.arduino.cc/ | v1.8.x | |
| Software, algorithms | PsychoPy | https://github.com/psychopy/psychopy/releases/tag/2020.1.1 (Peirce, 2020) | v2020.1.1 | |
| Software, algorithms | FIJI | https://imagej.net/software/fiji | N/A | |
| Software, algorithms | SHARP-Track | https://github.com/cortex-lab/allenCCF | N/A | |
| Chemical compound, drug | DiI (1,1’-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate) | Sigma-Aldrich | CAS# 41085-99-8 | |
| Chemical compound, drug | Streptavidin, Alexa 647 Conjugate | ThermoFisher | Cat# S21374 |
