Sleep need driven oscillation of glutamate synaptic phenotype
- International Institute of Integrative Sleep Medicine, University of Tsukuba, Japan
- Department of Neuroscience, Peter O’Donnell Brain Institute, University of Texas Southwestern Medical Center, United States
- Department of Psychiatry, Peter O’Donnell Brain Institute, University of Texas Southwestern Medical Center, United States
Figures
Figure 1
Sleep need-dependent responses of AMPA/NMDA ratio and silent synapses in the motor cortex.
(A) Examples of AMPA currents at –90 mV holding potential and NMDA currents at +50 mV holding potential are shown for: control sleep (CS), 6 hr of sleep deprivation (SD), and 4 hr of sleep …
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Figure 1—source data 1
EPSC subtype amplitudes (Tab 1); statistics for EPSC amplitudes X conditions (Tab2 & 3); failure rate data (Tab4); statistics for failure rate data (Tab5); and statistics ratios of failure rate data (Tab6).
- https://cdn.elifesciences.org/articles/98280/elife-98280-fig1-data1-v1.xlsx
Figure 2
snRNAseq data shows cell type and sub-type based on gene expression patterns are unaffected by sleep needs.
(A) Uniform Manifold Approximation and Projection (UMAP) projection of cell-type gene expression pattern following 6 hr ad lib sleep (control sleep, CS) at ZT = 6 hr. (B) As in (A) except after 6 hr …
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Figure 2—source data 1
Table of cell-type specific #'s of cells, genes and UMI's (Tab1); statistics for cell-type specific #'s of nuclei X condition (Tab 2); and statistics for cell-type specific # of UMI's X condition (Tab3).
- https://cdn.elifesciences.org/articles/98280/elife-98280-fig2-data1-v1.xlsx
Figure 3
Cell type-specific differential gene expression in response to 6 hr of sleep deprivation (SD).
(A) An XY bar plot of cell type specific differentially expressed genes (DEGs) (both up and down-regulated) organized by cell-type-specific number of expressed genes (X axis) shows the greatest …
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Figure 3—source data 1
Table of cell-type and condition specific #'s of genes and DEGs (Tab 1); and cell-type specific distribution of DEGs (Tab 2).
- https://cdn.elifesciences.org/articles/98280/elife-98280-fig3-data1-v1.xlsx
Figure 4
Differentially expressed gene (DEG) enrichment of cell types in response to sleep deprivation (SD) by autism risk genes, synaptic shaping component genes, and DEGs from MEF2c loss of function or constitutive HD4 repression of MEF2c.
(A) Heat map for cell type DEG enrichment by autism spectrum disorder (ASD) risk genes, Synaptic Shaping Component genes, MEF2c-cKO DEGs, and cnHD4 DEGs. (B) Model for the control of sleep DEGs by …
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Figure 4—source data 1
Cell-type specific DEGs from CS to SD, corresponding log2 fold change, and FDR (Tabs 1-4); and curated gene lists and the cell-type specific DEGs (>23% change of expression & FDR < 0.05) that overlapped with one of the curated lists (Tab5).
- https://cdn.elifesciences.org/articles/98280/elife-98280-fig4-data1-v1.xlsx
Videos
Video 1
