Figure 3. | An inhibitory corticostriatal pathway

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An inhibitory corticostriatal pathway

Figure 3.

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University of Texas at San Antonio, United States
Figure 3.
Download figureOpen in new tabFigure 3. Auditory CS-SOM neurons innervate both dSPNs and iSPNs.

(a) Experimental paradigm for photostimulating ChR2-positive CS-SOM projections while recording from genetically labeled dSPNs and iSPNs. (b) Left, epifluorescence image of dSPNs expressing D1-tdTomato (white arrows indicate CS-SOM ChR2-tdTomato-positive axons). Middle, epifluorescence image of iSPNs expressing D2-EGFP. Right, overlay of D1-tdTomato (dSPNs) and D2-GFP (iSPNs). Note that the two sub-types of SPNs have a different distribution with no overlap when located in the same region of the dorsal striatum (white arrows indicate CS-SOM ChR2-tdTomato-positive axons). (c) Left, high-resolution epifluorescence image of a biocytin-labeled dSPN. The dashed box indicates the location of the image in right panel. Right, high-resolution epifluorescence image of spines from the biocytin-labeled dSPN. (d) Example of IPSCs recorded at 0 mV from a dSPN (left) and iSPN (right) after application of ionotropic glutamate receptor antagonists (NBQX 10 μM, CPP 5 μM: red traces) and GABAA receptor antagonist (gabazine 25 μM: black traces). (e) Left, plot of IPSCs onset latencies recorded in dSPNs (n = 7; red circles) and iSPNs (n = 7; green circles), including group averages (± s.e.m.). Middle, plot of IPSCs peaks calculated for dSPNs (n = 7; red circles) and iSPNs (n = 7; green circles), including group averages (± s.e.m.). Right, plot of IPSCs charge calculated for dSPNs (n = 7; red circles) and iSPNs (n = 7; green circles), including group averages (± s.e.m.). (f) Example of IPSCs (black trace), rising time course (red trace) and decay time course (amber trace) recorded at 0 mV from a dSPN. (g) Left, plot of IPSCs rising time course calculated for dSPNs (n = 7; red circles) and iSPNs (n = 7; green circles), including group averages (± s.e.m.). Right, plot of IPSCs decay time course calculated for dSPNs (n = 7; red circles) and iSPNs (n = 7; green circles), including group averages (± s.e.m.).

DOI: http://dx.doi.org/10.7554/eLife.15890.005