Bosentan treatment improves axonal regeneration after peripheral nerve injury in adult mice.
A. Scheme of drug treatment and peripheral nerve injury model.
B. Quantification of the length of the 10 longest axons in indicated conditions.
C. Quantification of the regeneration index, calculated as the distance along the nerve where the SGC10 intensity is 50% of the SCG10 intensity at crush site.
D. Representative longitudinal sections of sciatic nerves 24h after SNC, immunostained for SCG10, from mice with the indicated treatment. Dotted line indicates the crush site, determined as the maximal SGC10 intensity (scale bars, 200 μm).
E. Quantificaiton of SCG10 intensity at the indicated distance normalized to the intensity at the crush site for each condition. N = 5 mice/condition.
F. Scheme of adult DRG neuronal culture and treatments.
G. Representative images showing TUJ1 (black) immunostaining of neurons in DRG cultures (scale bars, 100 μm).
H-I. Quantification of axonal radial length (K) and total TUJ1+ area (L). Different colors represent different biological replicates. N (neuron number) = 177 (vehicle; three biological replicates, naïve mice), 168 (Ambrisentan, three biological replicates, naïve mice), 183 (Bosentan; three biological replicates, naïve mice), 186 (vehicle; three biological replicates, injured mice), 204(Ambrisentan, three biological replicates, injured mice) and 210 (Bosentan; three biological replicates, injured mice), respectively. The data are presented as mean ± SD.