Abstract
Two types of osteoblasts are required to assemble the zebrafish embryonic skeleton: classical osteoblasts homologous to the mammalian cell, and notochord sheath cells, which serve as non-classical osteoblasts. The gene entpd5a is critically required for ossification via both types of osteoblasts. Despite the indispensability of zebrafish models in vertebrate research, the genetic regulation of bone formation, as well as mechanisms of transcriptional control of entpd5a, remain largely unknown. Here, using a newly generated transgenic line, we isolate classical and non-classical osteoblasts from zebrafish embryos and performed both ATAC-seq and RNA-seq. We analysed results independently and integratively to understand those chromatin dynamics and accompanying transcriptomic changes that occur in different skeletal cell types. We show that although Dlx family factors are playing important roles in classical osteoblast regulation, Hox family factors are involved in governing spinal ossification via non-classical osteoblasts. We further present a resource-driven analysis of the entpd5a promoter, experimentally validating the ATAC-seq dataset and proposing mechanisms of regulating the complex entpd5a expression pattern during zebrafish osteogenesis. Our results thus provide a necessary comprehensive resource for the field of bone development and indicate spatio-temporally regulated promoter/enhancer interactions taking place in the entpd5a locus.
Introduction
Osteogenesis is one of the vital processes taking place in vertebrate embryos. In amniotes, including all mammals, osteoblasts are known to give rise to all cranial and axial bones through endochondral and intramembranous ossification (Mackie et al., 2008, Breeland et al., 2022). While most cranial bones form via intramembranous ossification, mammalian axial bones are formed via a cartilagenous template by osteoblasts derived from the somitic sclerotome. Despite the differences in modes of ossification, all osteoblasts are considered as a uniform cell type, experiencing similar ontogenetic stages which are controlled by similar genetic mechanisms (Amarasekara et al., 2021; Hartmann, 2009; Jensen et al., 2010).
Teleost and amphibian processes of endochondral and intramembranous ossification are conserved with amniotes (Weigele and Franz-Odendaal, 2016). Although the osteoblasts, which generate bone in amniotes and anamniotes are considered homologous, relatively recent work in teleost fish identified a bone producing cell type, which has not been described in anamniotes: the notochord sheath cell (NSC) (Grotmol et al., 2005; Lleras Forero et al., 2018). NSCs, also referred to as chordoblasts, form an epithelial layer around the vacuolated cells of the embryonic notochord (Fig. 1A), and secrete the molecules making up the acellular, thick notochord sheath layer. NSCs are directly responsible for forming ossified chordacentra, ossified rings that are positioned in a segmental array within the sheath of the notochord. Chordacentra formation precedes the accumulation of sclerotomal osteoblasts around the notochord, which complete formation of the vertebral centra and in a later step also give rise to the dorsal and ventral arches (Fleming et al., 2015; Grotmol et al., 2005; Lleras Forero et al., 2018). Thus, formation of chordacentra is now considered the first stage of spine formation in fish.
Here we coin the terms “classical” and “non-classical” osteoblast to describe zebrafish bone formation. Classical osteoblasts, highly homologous to the mammalian osteoblast, express osterix and generate the cranial bones and the late perichordal centra of the spine, while non-classical osteoblasts, with to date no known homologous cell type in amniotes, refer to the osterix- subset of NSCs capable of early chordacentra mineralisation. Zebrafish cranial classical osteoblasts originate from the cranial neural crest, share a common progenitor with cranial chondrocytes, and can be detected as early as 2 dpf using markers such as runx2b, entpd5a and osterix (Huitema et al., 2012; Li et al., 2009). Axial classical osteoblasts are derived from the somitic sclerotome and contribute to the mineralization of the finite vertebrae in a process very similar to the one observed in mammals. They are rather late in appearance, and osterix+ cells can only be found along the growing vertebral central and arches from approximately 17 dpf onwards (Spoorendonk et al., 2008) (Fig. 1B, C).
Non-classical osteoblasts are derived from notochord progenitor cells (NPCs). Unlike classical osteoblasts, NSCs are distinct in their lack of expression of the transcription factor Osterix, commonly considered as an osteoblast master regulator in vertebrates (Nakashima et al., 2002; Wopat et al., 2018). Rather than osterix, expression of the early zebrafish bone marker ectonucleoside triphosphate diphosphohydrolase 5a (entpd5a) was demonstrated in bone-forming NSCs using BAC transgenic lines (Fig. 1D). As early as 4 dpf entpd5a was shown to be expressed in NSCs in a segmental pattern, gradually progressing from anterior to posterior, predicting the precise location of the mineralised chordacentra (Lleras Forero et al., 2018; Wopat et al., 2018). entpd5a+ segments along the notochord are separated by intersegmental regions expressing cartilage markers, such as col9a2 (Fig. 1E). entpd5a codes for a secreted hydrolase binding nucleoside di- and triphosphates, generating NMP and inorganic phosphate, a key mineral to initiate the mineralisation process of bone. Indeed, entpd5a mutant zebrafish embryos present with a complete lack of ossification (Huitema et al., 2012). In mouse mutants this phenotype has not been reported, although it was observed that homozygous mutants are smaller than their littermates (Read et al., 2009). Thus, NSCs, despite providing a morphologically uniform population of cells, are critical for initial chordacentrum ossification by expressing entpd5a in a segmental manner along the anterio-posterior axis. How this segmental expression pattern of entpd5a is established is not understood, but it is distinct from the one involved in somitogenesis (Lleras Forero et al., 2018). The role of the BMP and Notch signalling pathways has been highlighted in the segmenting notochord (Peskin et al., 2023; Wopat et al., 2018), but the transcriptional events governing entpd5a expression in classical osteoblasts and in the NSCs remain unknown.
The transcriptomics of mammalian classical osteoblasts have been investigated in some detail in the past decades. Genes such as Runx2, Dlx family factors and Osterix play significant roles in different stages of osteoblast specification and maturation (Hassan et al., 2004; Hojo et al., 2022; Jensen et al., 2010; Li et al., 2008; Shirakabe et al., 2001). More recently, the Assay for Transposase-Accessible Chromatin (ATAC-seq) has been widely used in the field. In addition, an increasing number of studies integrate RNA-seq and ATAC-seq to provide deeper insight into gene regulation in populations of interest (Ackermann et al., 2016; Hao et al., 2022; Hojo et al., 2022; Yang et al., 2021; Yu et al., 2021). Such studies, however, are to date available neither for classical, nor for non-classical zebrafish osteoblasts, rendering the molecular fingerprints and chromatin dynamics of zebrafish osteogenic cells to date elusive. Molecularly characterising zebrafish classical osteoblasts is of particular interest for the field, as it allows for direct comparison between mammalian and teleost transcriptional regulation of osteogenesis. Specifically, it is crucial to acquire a deeper understanding of teleost osteoblasts in order to answer evolutionary questions regarding the cell type’s origin (Nguyen and Eames, 2020). Moreover, better understanding the mechanisms of regulation in non-classical osteoblasts is likely to highlight either novel ossification mechanisms that have not been appreciated to date, or that are unique in teleosts.
In this study, we provide the first comprehensive resource of ATAC-seq and RNA-seq performed on classical and non-classical zebrafish osteoblasts, enabling studies of embryonic osteoblast development and differentiation. Comparisons across those populations and their closely related chondrocyte and intersegmental NSC populations allows a detailed assessment of gene expression profiles and chromatin regulation in different osteogenic cells, based on FAC-sorted cells from live embryos. We furthermore focus on the promoter structure of the early bone marker entpd5a, a gene critical for osteogenesis in zebrafish. We thus not only validate the usefulness and quality of the ATAC-seq data, but also demonstrate the complex mode of entpd5a regulation through identification of enhancers located both upstream of the ATG and within intronic regions, and suggest candidate transcription factors likely to be acting on the identified enhancer elements.
Results
entpd5a is an early marker of developing osteoblasts
Entpd5a is a critical factor for zebrafish osteogenesis, and entpd5a mutants show no bone formation at all. We had previously generated a transgenic reporter line and established the gene to be expressed at sites of bone formation, but wanted to better understand the dynamics of entpd5a expression. We thus recombined the BAC construct CH73-213B8, which was used previously to generate stable entpd5a transgenic lines (Bussmann and Schulte-Merker, 2011), and replaced the start codon of the gene with that of the Gal4FF open reading frame (Suppl. Fig. 1A). The Gal4/UAS system has proven valuable for other genes to provide stronger fluorescence signals. Indeed, the newly generated entpd5a:Gal4FF; UAS:GFP transgenic line (Labbaf et al., 2022) allowed detection of entpd5a expression domains which had previously not been reported.
Using this line, we could detect for the first time expression of entpd5a not only in the developing cranial bones starting from 3 dpf, but also in a subset of cartilage cells forming the developing jaw (Suppl. Fig. 1B, D). This pattern is reminiscent of the one previously shown for the bone master regulator osterix (Hammond and Schulte-Merker, 2009). In addition, we detected entpd5a expression in a few neurons of the CNS in the head at 3 dpf (Suppl. Fig. 1C) and along the spinal cord. We also found expression within the hypochord (data not shown). Finally, observation of the strongly expressing Gal4 line led us to identify early expression of entpd5a in notochord progenitor cells (NPCs) at 24 hpf (data not shown).
To further elucidate early entpd5a expression, we used the previously established entpd5a:Kaede transgenic line (Lleras Forero et al., 2018) to investigate notochord dynamics prior to 3 dpf. We photoconverted and directly imaged entpd5a:Kaede positive embryos starting from the 15 somite-stage (s), when we could first detect the fluorophore along the newly-formed notochord progenitor cells (Suppl. Fig. 1E). We repeated photoconversion and imaging at 18, 21 and 24s (Suppl. Fig. 1F-H). In all three stages, de novo expression (in green) was identified. We repeated the photoconversion and imaging at 48 hpf and at 72 hpf (Suppl. Fig. 1I-J). At both stages, expression of entpd5a was restricted exclusively to NSCs positioned along the ventral-most notochord sheath. Hence, the previously observed expression of entpd5a in the notochord prior to segmentation (Lleras Forero et al., 2018) in vacuolated cells and a subset of NSCs is merely the result of long half-lives of fluorophores.
In conclusion, entpd5a expression in the zebrafish embryo appears to be more dynamic than previously appreciated, which implies diverse regulatory mechanisms. A list of tissues expressing entpd5a (Table 1) provides a better understanding of entpd5a expression dynamics, and serves as a basis for considering ATAC- and RNA-sequencing datasets.
ATAC-seq on classical versus non-classical osteoblasts identifies a high degree of correlation between regions of open chromatin
In order to identify cell type-specific enhancers in the different cell types forming the zebrafish skeleton, and to further predict crucial transcriptional regulators that are active in each population, we performed ATAC-seq using cells isolated from the entpd5a:Gal4FF; UAS:GFP; R2col2a1a:mCherry transgenic line at 15 dpf (Suppl. Fig. 2A-B). The developmental stage was chosen in order to obtain sufficient numbers of entpd5a+ and entpd5a- NSCs (Suppl. Fig. 2A). Larvae were decapitated and dissociated, and cells were sorted according to fluorescence, using GFP-; mCherry-embryos as controls. Classical GFP+ osteoblasts and mCherry+ chondrocytes were isolated from the head and GFP+ NSCs and mCherry+ intersegmental NSCs from the trunk (Suppl. Fig. 3).
We assessed the quality of sequenced data according to ENCODE standards and used ATAQV (Orchard et al., 2020) to determine the Fragment Length Distribution Distance (FLDD) and the TSS enrichment score (Supplementary Table 1). The FLDD scores were always found to be between 0 and 0.3, indicating good degree of transposition. The TSS value, although low in comparison to data from mammalian cells, indicates enrichment of fragments around the promoters. Furthermore, we assessed sample correlation via Principal Component Analysis (PCA) and by producing a correlation heatmap based on FRiP (Fraction of Reads in Peaks) scores using DiffBind (Suppl. Fig. 2C-D). Both methods highlighted a high degree of correlation between biological replicates, as well as tight clustering between all 6 samples derived from entpd5a+ cells versus the 6 samples derived from entpd5a-cells. These correlations confirmed the reliability of the data.
Differential accessibility analysis was performed, comparing the two head-derived cell populations with each other to identify putative enhancer regions associated with either classical osteoblasts or with chondrocytes (Suppl. Fig. 2E). Similarly, the two trunk populations were compared to identify open chromatin regions specific to the entpd5a+ bone-producing NSCs, and chromatin regions specific to entpd5a- intersegmental NSCs (Suppl. Fig. 2F). Overall, we identified here 558 peaks specific to classical osteoblasts and 26 peaks enriched in NSCs (non-classical osteoblasts). The latter small number is likely due to the high degree of similarity of the two cell types compared (entpd5a+ vs. entpd5a-NSCs).
Functional validation of ATAC-seq leads to novel insights into entpd5a regulation
To test if the ATAC-seq open chromatin regions are predictors of putative regulatory elements, we focused on the entpd5a locus, and used GFP reporters to perform enhancer function tests in vivo. Within close proximity to the entpd5a ORF six ATAC peaks show enrichment in osteoblasts and represent enhancer candidates (Fig. 2A). Further away from the ORF, the non-coding sequences present in the BAC contain an additional 2 peaks of interest for osteoblast regulation (peaks 7-10, Fig. 2B) and 2 peaks enriched in cartilage samples (peaks 8/9, Fig. 2B). Using the ZF_carp_phastcons track on the UCSC browser (Baranasic et al., 2022), we assessed the degree of teleost non-coding sequence conservation in close proximity to the open reading frame of entpd5a. We found that all 10 ATAC-peaks were positioned within conserved non-coding regions, suggesting the presence of cis regulatory elements (Fig. 2B). We first focused on ATAC peaks 1-6 within approximately 10kb of the entpd5a ATG (Fig. 2A). As shown in the diagram, the first peak was found in an intron at the 3’ end of the coq6 gene. Peaks 2 and 3 were contained in introns 3 and 1, respectively, of the entpd5a gene. The first introns of a gene often play regulatory roles (Rose, 2019), making these peaks intriguing. Finally, we highlight peak 4, close to the TSS of entpd5a, (Nepal et al., 2013) as well as two more peaks (5 and 6) located within 6kb and 11kb of the TSS, respectively. We sought to experimentally test possible roles of these open chromatin domains, by generating transgenes that place these domains 5’ or 3’ of a GFP casette, and comparing the resulting expression patterns upon reporter transgenesis to that of the entpd5a locus (Table 1).
We first generated an entpd5a(2.2):GFP construct by cloning a 2.2kb fragment including the 5’UTR and core promoter of entpd5a as well as the non-coding region upstream of it, which includes peak number 4. The fragment was placed upstream of the GFP open reading frame (Fig. 2A), with Tol2 sites on either side of the construct. As a control, we generated entpd5a(1.1):GFP, a construct containing only the 5’UTR, along with the TSS and core promoter of the gene. We generated stable transgenic lines by injecting entpd5a(1.1):GFP or entpd5a(2.2):GFP together with Tol2 transposase mRNA into zebrafish eggs. In fish stably expressing entpd5a(1.1):GFP, expression could be detected in a variety of tissues but not in regions of ossification (data not shown). The result was confirmed by analysing progeny of six separate founders. In entpd5a(2.2):GFP; entpd5a:pkRed stable lines, we identified overlap of GFP and pkRed in the developing cranial bones, specifically the cleithrum, the operculum and a subset of jaw bones, but not in the notochord (Fig. 2C). While at 6 dpf segmentation in the notochord is ongoing, as can be readily observed using the entpd5a:pkRed stable line, GFP is undetectable in pkRed+ NSCs (Fig. 2C, inset). This result was consistently found in progeny of three different founders, indicating the presence of proximal enhancers of entpd5a functioning in cranial osteoblasts in the sequence where peak number 4 is identified.
In order to assess whether additional entpd5a enhancers are present upstream of the area covered by the entpd5a(2.2):GFP construct, we generated entpd5a(5.7):GFP, which extends a further 3.5kb, covering the peak number 5 sequence (Suppl. Fig. 4A). The sequence covered by the entpd5a(5.7):GFP construct is conserved across teleosts, but conservation is no longer observed 5’ of the cloned fragment (Fig. 2B). We generated stable transgenic lines, and imaged at 6 dpf in the background of the entpd5a:pkRed line, to identify cranial expression, as well as notochord expression (Suppl. Fig. 4B, C). As for entpd5a(2.2):GFP, we could only identify GFP expression in pkRed+ developing cranial bones, but not in the notochord (Suppl. Fig. 4B, C). Furthermore, although pkRed was detectable in the jaw chondrocytes, GFP signal was not found, indicating lack of a cartilage-specific enhancer capable of initiating expression autonomously within the sequence of peak number 5 (Suppl. Fig. 4B, inset). These results were validated by assessment of the progeny of eight founders. In conclusion, conserved sequences amongst teleosts upstream of peak number 4 do not appear to contain any enhancers that play roles in activating expression of entpd5a during development.
We next tested genomic regions equivalent to peaks 2 and 3, found in teleost-conserved entpd5a introns 3 and 1, respectively, for activating regulatory functions. To this end, we amplified the region between exon 1 and exon 4, including intron 1-3, and introduced them in the entpd5a(2.2):GFP construct, directly downstream of the GFP open reading frame, thus generating the construct entpd5a(2.2-introns):GFP. We injected the construct into single cell-stage zebrafish eggs, and observed that NSCs were expressing GFP in a mosaic manner (Fig. 3F-G), an effect not seen with other constructs. We generated an entpd5a(2.2-introns):GFP stable transgenic line, and indeed the notochord expression of entpd5a, as observed in the entpd5a:pkRed transgenic line, was present from 24 hpf until segmentation stages (Suppl. Fig. 5, Fig. 2D-F). Although GFP appeared in the expected segmental pattern along the notochord, expression levels were relatively low and patchy. This may be the result of this specific integration event (only one founder could be identified), or due to lack of additional enhancers located in other non-coding regions upstream or downstream of the gene. We confirmed, however, that the expression of GFP was enriched in segments compared to intersegmental spaces, by measuring the fluorescence intensity within 6 segments and 5 intersegmental spaces, respectively (Fig. 2F). Plotting the corrected total cell fluorescence measurements in each region (Fig. 2G) confirmed the segmental pattern of GFP in Fig. 2F.
Neither of the above constructs showed GFP expression in jaw cartilages, thus, a cartilage-specific enhancer of entpd5a has not been identified. We performed BAC recombineering to delete larger regions of the entpd5a:pkRed BAC, in order to assess presence of enhancers within peaks 6-10, as well as within the peak found in intron 11, which appears more prominent in cartilage cell populations (Fig. 2B, 3A). First, we generated the large deletion entpd5a(Δ21):pkRed, spanning 21.4kb upstream of the entpd5a(2.2):GFP construct, deleting the sequence harbouring peaks 5-10 from the BAC construct (Fig. 3A). We generated a stable transgenic line and found that the expression pattern appeared unaltered, with strong expression appearing in the cranial skeleton, cartilage and notochord (Fig. 3B-E). Therefore, no additional upstream open chromatin regions were found to contribute to the wild type expression pattern as depicted in Table 1. Moreover, we generated the deletion entpd5a(Δ10):pkRed, in which the intron 11 sequence was removed, but this failed to affect expression of pkRed in cartilages (data not shown).
Moreover, we went on to assess in a comparable in vivo setting the presence of active entpd5a enhancers within the region of peak number 1, located in the last intron of the gene coq6 (Fig. 2A). Similarly to the generation of entpd5a(2.2-introns):GFP, we generated the construct entpd5a(2.2-coq6):GFP (Fig. 3J), and injected it in single cell-stage zebrafish embryos. We scored the fish injected with either of the two constructs and found that NSCs were not expressing GFP in the case of entpd5a(2.2-coq6):GFP, while such cells were commonly occurring when the entpd5a(2.2-introns):GFP was injected (Fig. 3F-I, K).
We thus conclude that out of the 10 peaks of interest (Fig. 2B), only ATAC peaks 2, 3 and 4 (Fig. 2A) identify genomic elements playing activating roles in entpd5a regulation, with proximal enhancers in peak number 4 driving classical and regulatory elements in peaks 2 and 3 non-classical osteoblast expression. We further found that the open chromatin located further 5’ of the entpd5a TSS or downstream of the open reading frame plays no role in regulating expression in the developing cartilage and bone tissue of zebrafish embryos.
Identification of the classical osteoblast proximal enhancer region
In order to further narrow down the region within the entpd5a(2.2):GFP construct, which is responsible for driving cranial expression of entpd5a, we generated sequential deletions from the 3’ end of the construct. Constructs entpd5a(d243):GFP, entpd5a(d418):GFP, entpd5a(d665):GFP and entpd5a(d880):GFP carry deletions of 243bp, 418bp, 665bp and 880bp, respectively (Fig. 4A). Using the CIIIDER program (Gearing et al., 2019), which predicts TF binding sites along a sequence of interest, we identified two elements along the 2.2kb sequence that were predicted to contain putative binding sites of Runx2, a known regulator of osteogenesis. We wished to test whether these sites were functional for cranial osteoblast expression of entpd5a and generated three additional constructs, entpd5a(r37):GFP, entpd5a(r31):GFP and entpd5a(r37/r31):GFP, respectively carrying a 37bp deletion at the first Runx2 site, a 31bp deletion at the second Runx2 site, and both deletions simultaneously (Fig. 4A).
We injected the constructs into zebrafish embryos carrying entpd5a:pkRed at the single cell-stage, and screened at 3 dpf for GFP expression in the cleithrum, a morphologically distinct bone of the zebrafish skeleton. The total numbers of screened embryos with GFP+ cells along their bodies, indicating successful integration of the construct, are provided in Fig. 4J, and are derived from a minimum of 2 biological replicates for each construct. Each injection was accompanied by independent injections of the entpd5a(2.2):GFP control construct in sibling embryos. Of the GFP-expressing embryos, we scored embryos as positive when 2 or more GFP+ cells were present along the cleithrum at 3 dpf. When the entpd5a(2.2):GFP and the entpd5a(d243):GFP constructs were injected, 42.7% and 52.7% of the scored fish presented with strongly GFP-positive cleithra (Fig. 4B, C, J). The number of scored injected fish with GFP in their cleithra dropped to 12.7%, 0.5% and 0% when constructs entpd5a(d418):GFP, entpd5a(d665):GFP and entpd5a(d880):GFP, respectively, were injected (Fig. 4J). We observed that in those injections only very few GFP+ cells (if at all) were detected along the cleithra, in stark contrast to the control and entpd5a(d243):GFP constructs (Fig. 4B-F). This indicates that a region of 422bp (highlighted as a red box in Fig. 4A) is responsible for entpd5a regulation in classical osteoblasts.
The identified 422bp region of interest includes two putative Runx2 binding sites. We injected the three constructs, deleting them both individually and simultaneously, and found that, although deletion r31 had no effect in the number of scored embryos with GFP expression in the cleithrum at 3 dpf (44%), deletions r37 and r31/r37 both resulted in a consistent drop to approximately half of the control group (19.5% and 18.6%, respectively) (Fig. 4J). This led us to conclude that the predicted Runx2 binding site located close to the summit of the ATAC peak has a functional role in entpd5a expression in the cranial skeleton.
Integration of ATAC-seq and RNA-seq identifies candidate regulators of classical and non-classical osteoblasts
In order to identify possible transcriptional regulators active in cell types of interest, we sought to integrate our ATAC-seq dataset with differential expression data from RNA-seq. To that end, we performed RNA-seq at 15 dpf on cranial osteoblasts and cartilage chondrocytes, as well as on entpd5a+ NSCs and intersegmental entpd5a-NSCs. As with ATAC-seq, biological triplicates of all samples were sequenced and analysed.
Our RNA-seq results highlighted unique signatures, with entpd5a+ cells from head and trunk highly expressing genes known to function in bone formation, for example phex (Quarles, 2003), panx3 (Ishikawa and Yamada, 2017) and col10a1b. In contrast, entpd5a-cells from head and trunk were found to express genes known to function in cartilage formation, such as acan and ccn family genes (Dateki, 2017; John A. Arnott, 2016) as well as col9a1b (Fig. 5A, B). We further show that, as expected, entpd5a+ cells isolated from both tissues share 872 genes, accounting for 43% of the total differentially expressed genes (DEGs) of both head and trunk entpd5a+ cells (Fig. 5C). Similarly, entpd5a-cells share 765 genes, accounting for 64% of the total DEGs found in cartilage and 40% of those found in intersegmental cells. This high degree of overlap was in contrast to the very low number of genes shared between osteoblasts and intersegmental cells (43 DEGs) or between cartilage and entpd5a+ NSCs (7 DEGs). We confirmed the reliability of our results by performing PCA analysis, in which the 3 biological replicates of each cell type clustered together (Fig. 5D). Of note, Principal Component 1 (PC1) distinguishes between the samples isolated from head versus trunk, while PC2 effectively distinguished between entpd5a+ and entpd5a-cells from both tissues. Overall, we observed significant overlaps in gene expression signatures between the entpd5a+ cells and entpd5a-cells, even though they are distinctive cell types, originating from widely diverse progenitors. We do note, however, that despite the similarities, classical and non-classical osteoblasts maintain significant differences in their expression profiles.
In teleost fish, osteoblasts have been previously found to share expression of typical chondrocyte genes, providing support to theories of bone cells having evolved from cartilage cells (Nguyen and Eames, 2020). Looking closely at our RNA-seq data and ATAC-seq data we found that, despite the logFC values showing clear enrichment in entpd5a-cells, indeed classical chondrocyte markers maintain open promoters (Suppl. Fig. 6A-D) and low levels of gene expression relatively to cartilage and intersegmental cells (Suppl. Fig. 6E). It should be noted that ATAC signal indicating the presence of active enhancers in promoter areas is observably lower, often by an order of magnitude, in osteoblasts compared to cartilage cells (Suppl. Fig. 6A-D).
We next combined the information of the ATAC-seq and RNA-seq datasets, to gain further insight into gene regulatory activity in our cell types of interest. To this end, we searched within intronic regions, as well as (a) 5kb, (b) 10kb and (c) 20kb upstream of the TSS of all genes in the GRCz11 genome assembly and isolated the respective three sets of zebrafish genes associated with ATAC peaks. First, we made an assessment of the distribution of peaks in the vicinity of genes. For each cell population, we plotted the proportion of zebrafish genes that had ATAC peaks in close association with promoter regions (within 5kb of the TSS and in the introns), in comparison to the proportion of genes that only had ATAC peaks associated with sequences within 10kb or within 20kb of their TSS (Fig. 6A). We concluded that in all cell populations, the majority of identified genes had peaks close to their promoter regions or within the intronic domains. A relatively small percentage of genes showed peaks only associated with the area 5kb-10kb of the TSS or with the area 10kb-20kb of the TSS. The same conclusion was reached upon isolation from these three isolated sets of genes that are actively expressed in each cell population according to our RNA-seq data. We plotted the proportion of cell type-specific DEGs that are associated with cell type-specific ATAC peaks and found that an even larger proportion (in all populations over 80%) of identified DEGs had ATAC peaks in close proximity to their predicted TSS, compared to very few additional DEGs identified for having peaks in areas between 5kb-20kb of the TSS (Fig. 6B). This effect further validated our ATAC-seq results, showing that ATAC peaks were concentrated close to the TSS of genes, rather than being distributed evenly in non-coding regions of the genome. Furthermore, we observed that the proportion of genes with peaks within 5kb of their TSS increased when looking exclusively at DEGs, further supporting the higher degree of chromatin accessibility around their promoters, related to their active regulation.
Subsequently, we sought to identify the degree of overlap between genes that are associated with ATAC peaks either in their introns or up to 20kb upstream of the TSS, and differentially expressed genes (DEGs) in each cell type (Fig. 6C-F). We found 152 DEGs with ATAC peaks in cranial osteoblasts, 251 in cartilage, 8 in entpd5a+ NSCs and 56 in intersegmental cells. In 3 out of 4 cell types, there were significantly more DEGs identified, compared to genes associated with ATAC peaks, an effect perhaps associated with the exclusion of peaks during differential accessibility analysis.
We then extracted the ATAC peaks associated with DEGs that have open chromatin in close proximity to the TSS. We examined the extracted peaks using the HOMER software, in order to identify enriched transcription factor binding sites in promoters of interest in each cell population. We intersected the resulting tables with RNA-seq datasets, identifying zebrafish orthologues of the predicted binding transcription factors that are differentially expressed in each given cell type (Table 2). Interestingly, we found that in osteoblasts there was both an enrichment for predicted binding sites of factors belonging to the Dlx family, and higher expression of orthologues of those particular members of the family compared to cartilage cells. Similarly, Hox family transcription factors were predicted to bind to open chromatin close to DEGs in entpd5a+ segments. Simultaneously, the zebrafish orthologues of the identified family members were differentially expressed in entpd5a+ segments. Finally, our analysis highlighted an enrichment of Fox and Sox family binding sites in both cartilage and intersegmental cells, accompanied by differential expression of the corresponding family member orthologues in our RNA-seq samples.
Finally, we used CIIIDER to search within introns 1 and 3, as well as within the 422bp element of the entpd5a promoter element identified in this work to play a crucial role in driving cranial osteoblast expression (Fig. 4A), for predicted binding sites of the classical and non-classical osteoblast regulators shown in Table 2 (Fig. 7). Notably, 12 putative Dlx factor binding sites were identified along the 422bp promoter region, with two of them contained entirely within the sequence deleted using the entpd5a(r37):GFP construct (Fig. 4A). The same area included a predicted Isl1 binding site in classical osteoblasts and two Hoxc8 binding sites in non-classical osteoblasts (Fig. 7). A total of six Runx2 binding sites were identified, of which two were positioned in the 422bp sequence. Of these, one site was found to drive expression in classical osteoblasts (Fig. 4). Although Runx2 was not one of the candidate factors identified in osteoblasts (Table 2), it is a factor known to play important regulatory roles in osteoblasts (Hojo et al., 2022; Lee et al., 2010; Nishio et al., 2006; Rashid et al., 2014). Moreover, our RNA-seq data showed that both runx2a and runx2b are slightly upregulated in osteoblasts versus cartilage (logFC = 1.39 and 2.64, respectively) and runx2a is upregulated in entpd5a+ segments versus intersegmental cells (logFC = 1.69). Compatible with this, our ATAC-seq data showed open chromatin in the vicinity of both genes, likely indicating open enhancers (data not shown).
In conclusion, we identified a number of strong candidates that are likely to function as transcriptional regulators in zebrafish classical and non-classical osteoblasts and that guide the dynamic and diverse expression of zebrafish entpd5a in these cell types.
Discussion
In recent years, research on teleost fish identified the ability of the embryonic notochord to produce bone in a manner independent of classical sclerotome-derived osteoblasts, and to initiate the formation of mineralised chordacentra (Fleming et al., 2004; Lleras Forero et al., 2018). The cells responsible for this are non-classical osteoblasts within the notochord sheath, expressing the early bone marker entpd5a but not the classical osteoblast regulator osterix (Lleras Forero et al., 2018; Spoorendonk et al., 2008). Here, we aimed to better understand the genetic regulation and molecular signatures of classical and non-classical osteoblasts, and how those two populations differ from each other and from the closely related cranial chondrocytes and intersegmental NSCs. To this end, we characterised the newly generated transgenic line entpd5a:Gal4FF; UAS:GFP in combination with the previously described R2col2a1a:mCherry to isolate classical and non-classical osteoblasts as well as cartilage and intersegmental cells from the head and trunk of zebrafish larvae. We employed ATAC-seq to reveal the differentially accessible chromatin domains in entpd5a+ cells of head and trunk populations and performed RNA-seq in equivalent cell populations to understand the gene expression signatures accompanying observed chromatin changes. This first compilation of combined ATAC-seq and RNA-seq data from teleost osteoblasts enables the genome-wide analysis of osteoblast, chondrocytes, notochord sheath cells, and intervertebral disk precursors at both the chromatin and transcriptome level. This comprehensive dataset will be of use to a wide variety of researchers interested in osteogenesis, both within and outside the field of teleost developmental biology. For our immediate purposes, and as a proof of principle study, the data has allowed us to dissect the promoter organisation and to identify likely regulators of entpd5a, a critical gene in bone formation in zebrafish.
The ATAC-seq datasets of both entpd5a+ and entpd5a-cells in the head and trunk reveal the chromatin landscape in these skeletogenic cell populations. Although highly similar due to their respective origin from either cranial neural crest cells or notochord progenitor cells, differential chromatin accessibility analysis on head and trunk populations revealed putative enhancer regions of interest for bone vs. cartilage generation. Here, we validate our chromatin accessibility datasets and prove the significance of performing such analyses by demonstrating the regulatory importance of open regions detected both upstream of entpd5a and within the gene’s intronic regions, thus highlighting a highly complex promoter organisation. Specifically, we demonstrate that a series of active enhancers responsible for driving expression in classical osteoblasts are located in a short stretch of non-coding sequence within 2.2kb of the entpd5a ATG. Moreover, introns 1-3 contain regulatory regions required for entpd5a segmental activation in non-classical osteoblasts. Although we attempted to test several of the additional peaks found in non-coding areas surrounding the gene, more work is required to pinpoint the enhancers driving expression in cranial chondrocytes. In conclusion, the entpd5a:Gal4FF line has allowed us to refine the expression pattern of zebrafish entpd5a and to use ATAC-seq to elucidate the structure of its promoter.
We additionally performed RNA-seq in order to better understand the molecular signatures of the different cell populations and the functional significance of the observed chromatin configurations. As expected, our analyses reveal enrichment of bone-specific genes in entpd5a+ populations and, conversely, of cartilage-specific genes in entpd5a-populations. We went on to integrate the ATAC-seq and RNA-seq datasets to increase the predictive power of our analyses, which led to the determination of candidate transcriptional regulators for each cell type of interest. Our results suggest functional roles of Dlx family genes in classical osteoblasts, of Hox family regulators in non-classical osteoblasts, and of Foxa and Sox family members in entpd5a-cells of both the head and the trunk. It thus appears that gene expression is not regulated in the same manner in classical and non-classical osteoblasts, since the identified candidate regulators showed no overlap. In contrast, chondrocytes of the head and intersegmental NSCs demonstrated a surprising amount of overlap in putative regulators governing gene expression specific to their skeletogenic roles.
Identification of putative functions of Dlx family transcription factors in classical osteoblasts is compatible with the long-recognised roles of these factors in upregulating key osteoblast genes during all stages of osteoblast specification and maturation (Hassan et al., 2004; Li et al., 2008; Shirakabe et al., 2001). Significantly, Dlx factors have been implicated in recruiting Osterix, the master regulator of osteoblasts, to osteoblast enhancers in mice (Hojo et al., 2016). Interestingly, it has been shown that distinct Dlx family members become active in different stages of osteoblast development, with chick Dlx3 being able to activate mature osteoblast expression programs (Li et al., 2008). Consistent with this, our analyses reveal enrichment of dlx3b expression in zebrafish classical osteoblasts. In non-classical osteoblasts, we identified several Hox factors amongst predicted regulators of skeletal fate genes. Hox family genes have long been known for their crucial roles in skeletal patterning (Fromental-Ramain et al., 1996a, 1996b; Wellik and Capecchi, 2003), and in the adult skeleton (Song et al., 2020). In future work, the binding affinity of zebrafish Dlx and Hox factors on the promoters of osteoblast genes such as entpd5a, as well as generating mutants in specific dlx and hox genes would be appropriate means to obtain further insight into the gene regulatory network governing osteogenesis and axial patterning.
In regards to candidate regulators with putative roles in entpd5a-cells, Foxa2 and Foxa3 are transcription factors with confirmed roles in chondrogenesis (Ionescu et al., 2012). Furthermore, SOX9 is known as the master regulator of chondrocyte differentiation, and competition between SOX9 and FOXA have been shown to aid this process (Tan et al., 2018). Both entpd5a-cell populations investigated here differentially express the zebrafish genes foxa2, foxa3 and sox9a, while the second SOX9 orthologue, sox9b, is enriched in cranial entpd5a-cells. We thus postulate that the mechanism of chondrocyte differentiation is likely conserved between amniotes and zebrafish.
In summary, integration of ATAC-seq and RNA-seq has been valuable in predicting candidate regulators for skeletogenic cells found in fish in a non-biased manner. Although this should not be viewed as a comprehensive list of regulators, it provides guidance in the first steps of elucidating genetic interactions taking place in these cell types, and particularly in the non-classical osteoblasts of the notochord, which have only been recently started to be analysed.
In addition to its use in better understanding the gene regulatory programs acting in skeletogenic cells, our approach of performing both RNA-seq and ATAC-seq using in vivo-derived samples and analysing the data not only individually but also in an integrated manner, provides a comprehensive resource that has hitherto not been available. Although osteoblasts of mammals and birds have been the subject of transcriptomic analyses for decades, the vast majority of RNA-seq and ATAC-seq studies have been performed using in vitro-differentiated cells (Hao et al., 2022; Liu et al., 2022; Yu et al., 2021). To our knowledge, only one study thus far used in vivo-derived mouse neonatal skeletal cells to perform both assays (Hojo et al., 2022). Furthermore, RNA-seq and ATAC-seq studies on osteoblasts of teleost fish remain extremely limited or absent. With the exception of one study in which scRNA-seq was performed on the zebrafish adult regenerating fin (Tang et al., 2022), and another study where RNA-seq was performed on medaka osteoblasts (Phan et al., 2020), we are not aware of any RNA-seq or ATAC-seq analyses that have been performed on embryonic classical osteoblasts of fish. Thus, our study fills a void and moves teleost osteoblasts on par with the single mouse study (Hojo et al., 2022) that provided both ATAC-seq and RNA-seq data.
Of note, the significance of studying the molecular signatures of osteoblasts in teleost fish has become more pressing in recent years. It has been observed that ‘aquatic’ and ‘amphibian’ osteoblasts and cartilage behave differently to ‘terrestrial’ osteoblasts in terms of gene expression (Nguyen and Eames, 2020). Specifically, the less evolved osteoblasts of fish and amphibians appear to share gene expression with chondrocytes, a pattern which we also observed in our datasets, while amniote osteoblasts have suppressed chondrocyte markers in the course of evolution. Despite this, the vast majority of NGS studies have been carried out on osteoblasts of amniotes, leading to the establishment of an incomplete picture of the osteoblast (Nguyen and Eames, 2020). In this work, we show that the bone marker entpd5a is expressed in chondrocytes of the developing jaw, in a manner similar to osterix (Hammond and Schulte-Merker, 2009). Furthermore, we show that several other genes conventionally thought of as cartilage-specific, such as ccn6, sox9a, acana and col9a2 are expressed at low levels in osteoblasts. These discoveries reinforce the arguments regarding the chondrocyte origin of osteoblasts, while also pointing towards cells that might be prone to transdifferentiate into osteoblasts (Hammond and Schulte-Merker, 2009; Thesingh et al., 1991; Zhou et al., 2014).
Moving beyond the classical osteoblasts, we also here provide means to investigate non-classical osteoblasts, a cell type confined to teleosts that has recently been shown to play crucial roles in the initiation of fish spine development (Grotmol et al., 2003; Lleras Forero et al., 2018; Renn et al., 2013). Very little is known to date about the mechanisms of gene regulation in these non-classical osteoblasts, limited to RNA-seq data that indicate a role for BMP and Notch signalling in patterning the cells of the notochord sheath (Wopat et al., 2018; Peskin et al., 2023). However, the gene regulatory interactions that are required to bring about the segmental expression of entpd5a remains unknown. We here demonstrate and experimentally validate a short piece of promoter sequence to be sufficient in governing entpd5a expression in NSCs, setting the stage for identifying the gene regulatory network that determines transcriptional control of this key factor in axial skeletogenesis.
Finally, this work provides insight into the mechanism of regulation of the early bone marker entpd5a, which we show is regulated in an unexpectedly dynamic manner throughout zebrafish embryonic development. entpd5a is not only of great interest because it acts as a specific osteoblast marker, but also because it is non-dispensable for bone development in zebrafish (Huitema et al., 2012). Importantly, entpd5a expression presents in a segmented manner along the zebrafish embryonic notochord, predetermining the locations of chordacentra mineralisation and future vertebrae position. This segmentation was shown to be independent of the somitic segmentation clock (Lleras Forero et al., 2018), arguing for an independent manner of generating the periodic appearance of entpd5a expression within NSCs. The molecular mechanism guiding this stereotypic pattern remains unclear (Fernández Arancibia et al., 2022).
Here, we show that entpd5a introns 1-3, together with enhancers located upstream of the ATG, drive segmental notochord expression of entpd5a. Furthermore, we used our NGS analyses to predict candidate regulators, including dlx, hox and runx family genes, putatively binding on the identified enhancer sites to drive entpd5a expression in both classical and non-classical osteoblasts. Importantly, we identified a 37bp region within 2.2kb of the ATG, predicted to be able to bind Runx2a or Runx2b, as well as various members of the dlx gene family. runx2 family members are of particular interest to the process of osteogenesis, as mammalian Runx2 is known to play important roles in the regulation of osteoblast genes, both through its action as a pioneer factor, playing a crucial role in the plasticity between chondrocyte and osteoblast fates (Hojo et al., 2022), and through its interaction with co-factors to directly upregulate osteogenic genes (Lee et al., 2010; Nishio et al., 2006; Rashid et al., 2014). Although runx2a and runx2b were not thought to be expressed in mature osteoblasts in fish (Huycke et al., 2012), via RNA-seq data we detect low level expression of at least one of the two factors in entpd5a+ cells of the head and of the trunk, rendering them plausible entpd5a regulators in classical and non-classical fish osteoblasts. Our in silico predictions and presented hypotheses are to be validated by functional analyses (such as generating mutants), and this information can be built on to reveal gene regulatory network upstream of entpd5a in both types of zebrafish osteoblasts. Moreover, the role of Entpd5 in bone formation should be determined in other species and the evolutionary conservation of the mechanisms which we begin to identify here should be confirmed.
Overall, we here characterise the organisation of the complex promoter of entpd5a, and identify critical genomic elements that govern transcriptional control of this early and highly specific marker of classical and non-classical osteoblasts. En route, we suggest candidate transcriptional regulators that can now be tested. We furthermore provide a resource with a wide range of possible uses in the field, making both ATAC-seq and RNA-seq data sets available that are based on chondrocytes and osteoblasts isolated from in vivo derived material.
Materials and Methods
Fish raising and husbandry
Zebrafish (Danio rerio) strains were maintained according to FELASA recommendations (Aleström et al., 2020). Animal experiments have been performed according to animal ethics committees’ guidelines at the University of Münster, Germany. Tissues were collected according to the approved protocol for tissue removal. Embryonic developmental stages were determined according to Kimmel et al., 1995 (Kimmel et al., 1995). In this study, the following published transgenic lines have been employed: TgBAC(entpd5a:pkRed)hu7478and TgBAC(entpd5a:Kaede)hu6867 (Lleras Forero et al., 2018), Tg(UAS:GFP)nkuasgfp1a (Asakawa et al., 2008), Tg(R2col2a1a:mCherry)ue401Tg(Lopez-Baez et al., 2018), TgBAC(entpd5a:Gal4FF)mu412 (Labbaf et al., 2022).
Generation of constructs and transgenic lines
For generation of all lines, we amplified by PCR promoter sequences from zebrafish genomic DNA. The transgenic line Tg(−2.2entpd5a:GFP)mu416 (referred to as entpd5a(2.2):GFP) was generated by insertion of the PCR-amplified promoter sequence into a miniTol2-GFP vector via an EcoRI restriction site located directly upstream of the GFP. The line Tg(−2.2entpd5a:GFP-introns)mu419(referred to as entpd5a(2.2-introns):GFP) and the injected construct −2.2entpd5a:GFP-coq6 (referred to as entpd5a(2.2-coq6):GFP) were both generated by inserting PCR-amplified fragments downstream of the GFP cassette of the entpd5a(2.2):GFP vector via an EcoRV restriction site. Tg(−5.7entpd5a:GFP)mu418(referred to as entpd5a(5.7):GFP) was generated in a 2-step process, first using NEBuilder® HiFi DNA Assembly (New England Biolabs, E2621S) with the entpd5a(2.2):GFP construct, digested with MscI restriction enzyme, and 2 PCR-amplified fragments of 1023bp and 1040bp, respectively, as templates. The additional fragments were thus consecutively inserted directly upstream of the 2.2kb sequence. Subsequently, an 1.4kb PCR-generated fragment was inserted via a BamHI restriction site directly upstream of the previously inserted entpd5a promoter elements. All primers used for cloning are listed in Supplementary Table 2.
The deletion constructs shown in Fig. 4 were generated by PCR amplification of the entpd5a(2.2):GFP vector using phosphorylated primers listed in Supplementary Table 2. The PCR constructs were self-ligated to generate injection vectors. All lines were generated by injecting 25pg of plasmid DNA and 25pg of Tol2 transposase mRNA into single cell-stage embryos.
The TgBAC(Δ21entpd5a:pkRed)mu420 and TgBAC(Δ10entpd5a:pkRed)mu420lines (referred to as entpd5a(Δ21):pkRed and entpd5a(Δ10):pkRed, respectively) were generated using BAC recombineering based on the protocol previously described (Bussmann and Schulte-Merker, 2011). Briefly, Escherichia coli carrying the entpd5a:pkRed_Tol2 BAC construct were electroporated with a PCR-amplified sequence in which the open reading frame (ORF) of the antibiotic Spectinomycin is positioned downstream of the Kanamycin promoter. Flanking the Kan/Spectinomycin sequence were homology arms matching either side of the stretch of non-coding sequence targeted for deletion. The Spectinomycin ORF was thus inserted in the entpd5a:pkRed construct, replacing 21kb of non-coding sequence upstream of the ATG of entpd5a (entpd5a(Δ21):pkRed), or 10kb of coding and intronic sequences (entpd5a(Δ10):pkRed). 100pg of modified BAC DNA were injected into single cell-stage embryos, together with 25pg Tol2 transposase mRNA.
Imaging and image analysis
Confocal imaging was performed using a Leica SP8 microscope (10x and 20x objectives) employing Leica LAS X 3.5.6.21594 software (https://www.leica-microsystems.com/). z-stacks for the 20x objective were taken at an interval of 2.5μm. Stacks were flattened by maximum projection, images were processed and fluorescent measurements were performed using Fiji (Fiji Is Just ImageJ) (Schindelin et al., 2012). Adobe Photoshop CC 2022 was also used for image processing. Photo-conversion was performed on embedded TgBAC(entpd5a:Kaede) embryos via exposure for 5min to UV light.
FACS and RNA-sequencing
Embryos at 15 dpf were collected, anaesthetised and heads were removed through a clean cut directly anterior to the swim bladder. Heads and trucks were collected separately and incubated for 1hour at 34°C, at 400rpm in 0.25% Trypsin/EDTA in PBS (VWR, L0910-100) containing Collagenase B (Roche, 11088831011; 1% for head dissociation and 2% for trunk dissociation). Upon full dissociation, cells were centrifuged at 1,400rpm for 5min at room temperature. The supernatant was discarded and cells were washed with 1ml L15 medium (Sigma-Aldrich, L5520)/10% FCS solution. The wash step was repeated and samples of the same tissue were combined into a single Eppendorf tube in 1ml L15/FCS wash solution. Upon centrifugation and removal of the supernatant, pellets were resuspended in 1ml wash solution. Cells were passed through a 50μm and subsequently a 30μm strainer, and transferred to a FACSAria IIIu cell sorter (BD Biosciences) on ice. For FACS sorting the FACSDiva 8.0.1 (https://www.bdbiosciences.com/) and FlowJo 10.6.1 (https://www.flowjo.com/) software was used. For ATAC-seq and RNA-seq samples the 100μm and the 70μm nozzle were used, respectively. For ATAC-seq cells were sorted into ice-cold L15/FCS medium at 4°C, while for RNA-seq cells were collected directly into ice-cold RSB/β-Mercaptoethanol lysis buffer (QIAGEN RNeasy Plus Micro Kit, 74034; prepared according to manufacturer’s instructions).
For RNA-sequencing, RNA was isolated using the RNeasy Plus Micro Kit (QIAGEN, 74034). Samples were sent to Eurofins Genomics for library preparation and paired-end sequencing with the INVIEW Transcriptome Ultra Low service (2x 150). Per sample, 15M reads were sequenced. De-multiplexed sample files were obtained.
ATAC-sequencing
ATAC-seq was performed according to previously published protocols (Buenrostro et al., 2015), with the following modifications. Cells were incubated in lysis buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% Tween20, 0.1% NP40, 0.01% Digitonin) for 3min on ice. Lysis was stopped by resuspending in ice-cold wash buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% Tween20) prior to centrifugation. Depending on the numbers of cells isolated in each sample, we adjusted the volume of enzyme added to our transposition reaction (1x Tagment DNA Buffer, 0.01% Digitonin, 0.1% Tween20, TDE1 enzyme) and the length of transposition on a heat block set at 37°C shaking at 1,000rpm (Table 3). The Tagment DNA Buffer and TDE1 enzyme are both contained in the Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina, 20034197). The transposed chromatin was isolated using the Minelute PCR Cleanup kit (Qiagen, 28004).
All PCR amplification and quality control steps were carried out according to Buenrostro et al., 2015 (Buenrostro et al., 2015). Following amplified DNA purification, AMPure XP SPRI beads (Beckman Coulter, A63881) were used (1.4x sample volume) to remove primer dimers from the chromatin sample. The beads were brought to room temperature and thoroughly vortexed before use. The sample plus beads mixture was incubated at room temperature for 5min, then placed on the magnetic stand for 5min to clear the solution. The supernatant was discarded and the beads were washed for 30sec with 80% ethanol. The wash step was repeated. Following removal of the supernatant, the beads were air-dried, removed from the magnetic stand and 20μl of EB buffer was added for DNA elution. The sample was mixed and incubated for 2min at room temperature, then placed back on the magnetic stand for 5min. The supernatant was transferred to a fresh tube.
The quality of purified chromatin was assessed using the Bioanalyzer High Sensitivity DNA Analysis kit (Agilent). Libraries were sequenced using the NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) (Illumina, 20024904) on the sequencing platform Illumina NextSeq500. 20M reads were sequenced per sample.
Sequencing Data analysis
For ATAC-seq, sequences were de-multiplexed into fastq files applying standard parameters of the Illumina pipeline (bcl2fastq) using Nextera index adapters. The individual fastq files were processed with fastp (version 0.20.0) (Chen et al., 2018) and mapped to the zebrafish reference genome GRCz11 using Bowtie2 (version 2.3.5.1) (Langmead and Salzberg, 2012) with the ‘very-sensitive option’. Sequences with mapping quality >30 were isolated and sorted using SAMtools (version 1.9) (Danecek et al., 2021). Mitochondrial reads and duplicates were removed using BEDTools (version 2.28.0) (Quinlan and Hall, 2010) and Sambamba (version 0.7.1) (Tarasov et al., 2015), respectively. Reads were shifted using the standard method for ATAC samples and bigwig files were generated for visualisation using deepTools (version 3.3.1) (Ramírez et al., 2016). IGV (https://igv.org/app/) was employed for peak visualisation. MACS2 (version 2.2.6) (Zhang et al., 2008) was used to call peak enrichment with the settings -g 1.412e9 --keep-dup all --nomodel --shift −100 --extsize 200. ataqv (version 1.3.0) (Orchard et al., 2020) was employed for assessing the fragment distribution analysis and TSS enrichment scores. To determine differentially accessible chromatin regions and produce plots, we used the R package DiffBind (version 3.8.4) (Ross-Innes et al., 2012). The summits parameter was set to 150, to account for peak read lengths of 301bp. Head samples (osteoblasts vs. cartilage) and trunk samples (entpd5a+ segments vs. intersegmental regions) were individually contrasted. An element was considered entpd5a-positive if log2 fold change in read density was ≤-1 and FDR≤0.05. Conversely, entpd5a-negative elements were log2 fold change ≥1 and FDR≤0.05.
RNA-seq analysis was carried out by first pre-processing reads and removing duplicates using fastp (version 0.23.2) (Chen et al., 2018), aligning the cleaned reads to the zebrafish reference genome GRCz11 using the STAR aligner (version 2.7.2b) (Dobin et al., 2013), isolating and sorting high mapping quality reads (q > 30) using SAMtools (version 1.9) (Danecek et al., 2021). Raw read counts for each gene were calculated using the function featureCounts from the Subread package (Liao et al., 2014). The R package DESeq2 (version 1.38.3) (Love et al., 2014) was employed for differential expression analysis with an adjusted p-value cut-off of ≤0.05. As for ATAC-seq, entpd5a+ and entpd5a-populations from head and trunk samples were contrasted amongst each other, and only genes with |log2FC|>0.5 were considered for further analyses. PCA plots were produced with DESeq2, and heatmaps using the package ComplexHeatmap (Gu, 2022; Gu et al., 2016).
Integration of ATAC-seq and RNA-seq and binding site prediction
Data integration was performed using the R package tidyverse (Wickham et al., 2019). Visualisations were generated using the R packages VennDiagram (Chen and Boutros, 2011) and ggplot2. HOMER software was used to identify enrichment in transcription factor binding sites (Heinz et al., 2010). The program CIIIDER was used to identify binding sites within the entpd5a promoter sequences (Gearing et al., 2019).
Statistics, graphs and reproducibility
All images shown in the figures are representative examples of the respective expression patterns. All statistics were performed using R. For visualisations, we used the R package ggplot2.
Data availability
Processed as well as unprocessed ATAC-seq and RNA-seq data generated as part of this work can be accessed via Gene Expression Omnibus (accession numbers GSE267332 and GSE267333, respectively). Furthermore, code used in data processing has been deposited, and is freely available on Github (https://github.com/KleioP/ATAC_RNAseq_analysis/tree/Petratou-et-al.%2C-2024).
Acknowledgements
We thank Dr. Backialakshmi Dharmalingam, Dr. Kishor K. Sivaraj and Katja Müller (MPI Münster) for support with performing ATAC-seq, as well as Paul-Georg Majev (MPI Münster) and Dr. Ada Jimenez-Gonzalez (University of Birmingham) for their valuable feedback on analysing NGS data.
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