Sequence and structural homology of PfHO.

A) Sequence alignment of P. falciparum (PfHO, Q8IJS6), cyanobacterial (SynHO1, P72849), and human (HuHO1, P09601) heme oxygenase homologs (Uniprot ID). Conserved histidine ligand and distal helix residues required for catalysis in SynHO1 and HuHO1 are marked in red, and identical residues in aligned sequences are in gray. Asterisks indicate identical residues in all three sequences. The predicted N-terminal signal peptide of PfHO is underlined, electropositive residues in the PfHO leader sequence are highlighted in cyan, and the black arrow marks the putative targeting peptide processing site. Colored bars below the sequence alignment mark locations of α helices observed in crystal structures of PfHO (blue), SynHO1 (orange), and HuHO1 (grey), and the AlphaFold structural prediction for PfHO (purple). B) Structural superposition of the 2.8 Å-resolution X-ray crystal structure of apo-PfHO84–305 (blue, PDB: 8ZLD) and the 2.5 Å-resolution X-ray structure of cyanobacterial, SynHO1 (orange, PDB: 1WE1). C) Structural superposition of the proximal helix for SynHO1 active site (orange), PfHO crystal structure (blue), and the AlphaFold structural prediction of PfHO (purple). D) Top-scoring protein structures in the PDB identified by the DALI server based on structural similarity to the X-ray crystal structure of PfHO84–305. RMSD is calculated in angstroms (Å), and Z-score is a unitless parameter describing similarity, where greater value indicates higher similarity32.

Figure supplement 1. Sequence homologs of PfHO based on BLAST19 and HMM20 searches.

Figure supplement 2. Phylogenic tree of mammalian, plant, algal, and hematozoan HOs.

Figure supplement 3. X-ray crystallographic data collection and structure refinement statistics for PfHO.

Figure supplement 4. Sequence and structural alignments of PfHO to plant HOs (AtHO1, GmHO1).

Figure supplement 5. HO surface charge features.

Source data 1. PDB file for 2.8 Å-resolution structure of PfHO.

PfHO localization and processing.

A) Widefield fluorescence microscopy of Dd2 parasites episomally expressing PfHO-GFP. For live imaging, parasites were stained with 25 nM Mitotracker Red and 10 nM Hoechst. For IFA, parasites were fixed and stained with anti-GFP and anti-apicoplast ACP antibodies, as well as DAPI. For all images, the white scale bars indicate 1 µm. The average Pearson correlation coefficient (rp) of red and green channels based on all images is given. Pixel intensity plots as a function of distance along the white line in merged images are displayed graphically beside each parasite. B) Western blot of untreated or Dox/IPP-treated parasites episomally expressing PfHO-GFP and stained with anti-GFP antibody. C) Live microscopy of PfHO-GFP parasites cultured 7 days in 1 µM doxycycline (Dox) and 200 µM IPP and stained with 25 nM Mitotracker Red and 10 nM Hoechst. D) Widefield fluorescence microscopy of Dd2 parasites episomally expressing PfHO N-term-GFP and stained as in panel A. E) Western blot of parasites episomally expressing PfHO N-term-GFP and stained with anti-GFP antibody. F) Live microscopy of PfHO N-term-GFP parasites cultured 7 days in 1 µM Dox and 200 µM IPP, and stained as in panel C. For each parasite line and condition, ≥20 parasites were analyzed by live imaging and ≥10 parasites were analyzed by IFA.

Figure supplement 1. Additional widefield fluorescence microscopy of live Dd2 parasites episomally expressing PfHO-GFP and PfHO N-Term-GFP.

Figure supplement 2. Additional widefield IFA microscopy of fixed Dd2 parasites episomally expressing PfHO-GFP and PfHO N-Term-GFP.

Source data 1. Uncropped western blots of untreated or Dox/IPP-treated parasites episomally expressing PfHO-GFP in figure 2B.

Source data 2. Uncropped western blots of untreated parasites episomally expressing PfHO N-term-GFP in figure 2E.

PfHO is essential for parasite viability and apicoplast maintenance.

A) Western blot of untreated or Dox/IPP-treated parasites with endogenously tagged PfHO-GFP-DHFRDD. B) Immunogold TEM of a fixed 3D7 parasite endogenously expressing PfHO-GFP-DHFRDD and stained with anti-GFP (12 nm, white arrows) and anti-apicoplast ACP (18 nm) antibodies. C) Synchronized growth assay of Dd2 parasites tagged at the PfHO locus with the aptamer/TetR-DOZI system and grown ± 1 µM aTC and ± 200 µM IPP. Data points are the average ±SD of biological triplicates. Inset: western blot analysis of PfHO expression for 100 µg total lysates from parasites grown 3 days ±aTC, analyzed in duplicate samples run on the same gel, and stained with either custom anti-PfHO antibody or anti-heat shock protein 60 (HSP60) as loading control. Densitometry of western blot bands indicated >80% reduction in PfHO expression. D) Live microscopy of PfHO-aptamer/TetR-DOZI parasites episomally expressing apicoplast-localized GFP (PfHO N-Term-GFP) grown 5 days ±aTC with 200 µM IPP. White scale bars in bottom right corners are 1 µm. Right: Population analysis of apicoplast morphology scored for punctate versus dispersed GFP signal in 110 total parasites from biological triplicate experiments. Statistical significance was calculated by Student’s t-test. E) Quantitative PCR analysis of the apicoplast: nuclear genome ratio for PfHO-aptamer/TetR-DOZI parasites cultured 5 days ±aTC with 200 µM IPP, based on amplification of apicoplast (SufB: Pf3D7_API04700, ClpM: Pf3D7_API03600, TufA: Pf3D7_API02900) relative to nuclear (STL: Pf3D7_0717700, I5P: Pf3D7_0802500, ADSL: Pf3D7_0206700) genes. Indicated qPCR ratios were normalized to +aTC and are the average ±SD of biological triplicates. Significance of ±aTC difference was analyzed by Student’s t-test.

Figure supplement 1. Schemes for modification of the PfHO genomic locus to integrate the C-terminal GFP-DHFRDD or HA2-glmS tags.

Figure supplement 2. IFA microscopy of 3D7 parasites expressing PfHO-GFP-DHFRDD.

Figure supplement 3. Additional immunogold TEM images of 3D7 parasites expressing PfHO-GFP-DHFRDD.

Figure supplement 4. Scheme for modification of the PfHO genomic locus to integrate the aptamer/TetR-DOZI system.

Figure supplement 5. Validation of custom PfHO antibody specificity.

Figure supplement 6. Quantitative PCR and additional western blot analysis of PfHO expression ±aTC with 200 µM IPP.

Figure supplement 7. Giemsa-stained smears of PfHO-aptamer/TetR-DOZI parasites grown in ±aTC.

Figure supplement 8. Additional fluorescence microscopy images of PfHO-aptamer/TetR-DOZI parasites episomally expressing apicoplast-localized GFP grown 5 days ±aTC with 200 µM IPP.

Source data 1. Uncropped western blots of parasites with endogenously tagged PfHO-GFP-DHFRDD.

Source data 2. Uncropped western blots of PfHO expression.

Source data 3. Uncropped Southern blot of parasite DNA from PfHO-GFP-DHFRDD cultures.

Source data 4. Uncropped PCR gel of parasite DNA from PfHO-HA2-glmS cultures.

Source data 5. Uncropped PCR gel of parasite DNA from PfHO-aptamer/TetR-DOZI cultures.

Source data 6. Uncropped Southern blot of parasite DNA from PfHO-aptamer/TetR-DOZI cultures.

Source Data 7. Uncropped western blot of 3D7 parasites stained with rabbit serum prior to inoculation with PfHO protein antigen.

Source Data 8. Uncropped western blot of E. coli expressing PfHO84–305 and 3D7 parasites stained with crude serum from the final bleed of a rabbit inoculated with PfHO protein antigen.

Processing and essentiality of the PfHO N-terminus.

A) Western blot of lysates from Dd2 parasites endogenously expressing PfHO-HA2 and from E. coli recombinantly expressing PfHO84–305–HA2. B) AlphaFold structure and electrostatic surface charge predicted for PfHO33–305 corresponding to mature PfHO after apicoplast import. C) Synchronized growth assays of PfHO knockdown Dd2 parasites complemented by episomal expression of the indicated PfHO constructs in ±1 µM aTC. Growth assay data points are the average ±SD of biological triplicates.

Figure supplement 1. Peptide coverage of PfHO sequence detected by mass spectrometry.

Figure supplement 2. RT-qPCR analysis of endogenous PfHO transcript levels ±aTC in PfHO-aptamer/TetR-DOZI parasites complemented with PfHO episomes.

Figure supplement 3. Western blot of episomal PfHO expression in PfHO-aptamer/TetR-DOZI parasites.

Figure supplement 4. Sequence alignment of alveolate HO-like proteins indicating α-helical structure.

Source data 1. Uncropped western blots of parasites endogenously expressing PfHO-HA2 and E.coli expressing PfHO HO-like domain (PfHO84–305–HA2).

Source data 2. Uncropped western blot of episomal PfHO expression in PfHO-aptamer/TetR-DOZI parasites.

PfHO interactions with proteins and DNA and impacts of its knockdown on apicoplast DNA and RNA levels.

A) Spectral counts of functionally annotated, apicoplast-targeted proteins detected in two independent anti-HA IP/MS experiments on endogenously tagged PfHO-HA2. The names of proteins in DNA/RNA metabolism are highlighted blue and proteins in translation are highlighted in red. A list of all detected proteins can be found in Source Data 1. B) Representative image showing PCR amplification of nuclear-encoded (apicoplast ACP: Pf3D7_0208500) and apicoplast-encoded (SufB: Pf3D7_API04700) genes from DNA co-purified with full-length PfHO-GFP, PfHO1–83-GFP, ACPL-PfHO84–305–GFP, or ACPL-GFP by αGFP ChIP. “Input” is total parasite DNA collected after parasite lysis and sonication, and “ChIP” is DNA eluted after αGFP IP. Densitometry quantification of 3 biological replicates is plotted on right. Statistical significance of differences between PfHO and each other construct was calculated by Student’s t-tests. C) Quantitative PCR analysis of DNA isolated from tightly synchronized PfHO-aptamer/TetR-DOZI parasites grown ±1 µM aTC with 200 µM IPP and harvested at 36 and 84 hours in biological triplicates, with normalization of Ct values averaged from three apicoplast genes (SufB, TufA, ClpM) to Ct values averaged from three nuclear (STL, I5P, ADSL) genes. Grey bars represent +aTC and red bars represent –aTC, and observed ratios are displayed as percentages. D) Quantitative RT-PCR on RNA isolated from the same parasites as in panel C to determine the normalized ratio of apicoplast transcripts (SufB, TufA, ClpM) relative to nuclear (STL, I5P, ADSL) transcripts. Significance of ±aTC differences for C and D were analyzed by Student’s t-test. E) Representative time-course showing Ct values of SufB normalized to three nuclear (STL, I5P, ADSL) genes at indicated time in PfHO-aptamer/TetR-DOZI parasites grown ±1 µM aTC with 200 µM IPP. Data points are the average ±SD of biological triplicates.

Figure supplement 1. Spectral counts for proteins co-purified with PfHO in IP/MS experiments.

Figure supplement 2. List of apicoplast-localized proteins that co-purified with PfHO in IP/MS experiments.

Figure supplement 3. Functional pathway predictions for apicoplast-localized proteins that co-purified with PfHO in IP/MS experiments.

Figure supplement 4. Fragment analyzer quantification of parasite DNA fragment size after shearing.

Figure supplement 5. Steady-state PCR amplification of additional nuclear and apicoplast genes from DNA co-purified with indicated PfHO constructs.

Figure supplement 6. Additional ChIP experiments in parasites with GFP-tagged PfHO constructs.

Figure supplement 7. RT-qPCR of additional apicoplast genes in PfHO-aptamer/TetR-DOZI parasites grown for 3 days ±1 µM aTC with 200 µM IPP.

Figure supplement 8. Representative time-course of ClpM: nuclear transcript levels in synchronous PfHO-aptamer/TetR-DOZI parasites grown ±1 µM aTC with 200 µM IPP.

Source data 1. Table of proteins identified in PfHO IP/MS experiments.

Source data 2. Uncropped PCR gel of PfHO ChIP experiments in parasites with GFP-tagged PfHO constructs

Source data 3. Uncropped PCR gel of PfHO ChIP experiments ±crosslinking

Model for essential PfHO function in apicoplast genome expression and organelle biogenesis. TP = transit peptide. Scissors represent proteolytic processing of the PfHO N-terminal TP upon apicoplast import.

Supplementary file 1. Table of PCR primers.

Supplementary file 2. Table of reagents

Sequence homology of PfHO. (A) Sequence homologs of PfHO based on BLAST19 and HMM20 sequence-similarity searches. (B) Sequence alignment of PfHO (Q8IJS6) with SynHO1 (P72849), Human HO1 (P09601), and HO homologs in Theileria orientalis (J4C2V8) and Babesia microti (A0A1R4ACC9). Uniprot accession codes given in parentheses.

Phylogenic tree of mammalian, plant, algal, and hematozoan HOs. Nodes are annotated with bootstrap values for each branch. Ortholog groups independently predicted by OrthoMCL127 are annotated for each colored section. Only select Plasmodium HOs are included for clarity, but all other known proteins of OG6_156412 are displayed. PfHO is marked in red.

X-ray crystallographic data collection and structure refinement statistics for PfHO. Statistical values given in parentheses refer to the highest resolution bin.

Sequence and structural alignment of PfHO and plant HOs. A) Sequence alignment of PfHO with Arabidopsis thaliana (O48782) and Glycine max (C6THA4) HO1s (Uniprot ID). Heme-coordinating histidine and distal helix glycines in plant HOs are highlighted in red. B) Sequence identity of PfHO with proteins identified in our sequence homology analysis showing N-terminal targeting sequence and HO-domain separately. C) Structural alignment of the 2.8 Å-resolution PfHO crystal structure (blue, PDB: 8ZLD) with X-ray structures of A. thaliana HO1 (green, PDB: 7EQH) and G. max HO1 (yellow, PDB: 7CKA).

HO surface charge features. Electrostatic surface potential maps calculated for human HO1 (PDB 1N45), SynHO1 (PDB 1WE1), and the AlphaFold-predicted structure for PfHO (HO domain only) and contoured at ±5 kT/e. Calculations were performed using the APBS PDB2PQR online software suite131.

Additional widefield fluorescence microscopy of live, untreated or Dox/IPP-treated Dd2 parasites episomally expressing (A) PfHO-GFP or (B) PfHO N-term-GFP and stained with 25 nM Mitotracker Red and 10 nM Hoechst.

Additional widefield immunofluorescence microscopy of fixed Dd2 parasites episomally expressing PfHO-GFP and PfHO N-Term-GFP and stained with anti-GFP and anti-apicoplast acyl carrier protein (ACP) antibodies, and DAPI. White scale bars in bottom right corners are 1 µm.

Uncropped western blots of untreated or Dox/IPP-treated parasites episomally expressing PfHO-GFP, stained with goat anti-GFP primary and anti-goat-IRDye800 secondary antibodies, and visualized on a Licor Odyssey CLx imager.

Uncropped western blots of untreated parasites episomally expressing PfHO N-term(1-83)-GFP, stained with goat anti-GFP and mouse anti-hDHFR primary antibodies and anti-goat-IRDye800 and anti-mouse-IRDye680 secondary antibodies, then visualized on a Licor Odyssey CLx imager.

Schemes for modification of the PfHO genomic locus to integrate C-terminal GFP-DHFRDD or HA2-glmS tags. A) Schematic of single-crossover strategy for tagging PfHO gene locus with C-terminal GFP-DHFRDD. 1kb sequence at 3’ of PfHO coding region (green line) was used as a probe to test for integration by Southern blot, and enzyme digestion sites with expected sizes are indicated. B) Southern blot of digested parasite DNA harvested from wildtype, polyclonal PfHO-GFP-DHFRDD, and select clonal PfHO-GFP-DHFRDD cultures. C) Schematic of single-crossover integration strategy for tagging PfHO gene locus with C-terminal HA2-glmS. Primer sites used to probe integration by genome PCR are indicated. D) Genome PCR of parasite DNA harvested from wildtype parental and PfHO-HA2-glmS cultures.

Widefield immunofluorescence microscopy of fixed 3D7 parasites endogenously expressing PfHO-GFP-DHFRDD and stained with anti-GFP and anti-apicoplast acyl carrier protein (ACP) antibodies, and DAPI. White scale bars in bottom right corners are 1 µm. Pearson correlation coefficient (rp) of red and green channels is shown in merged images in yellow.

Additional immunogold transmission electron microscopy images of apicoplasts from fixed 3D7 parasite endogenously expressing PfHO-GFP-DHFRDD and stained with anti-GFP (12 nM, green arrows) and anti-apicoplast ACP (18 nM) antibodies.

Scheme for modification of the PfHO genomic locus to integrate the aptamer/TetR-DOZI system. A) Schematic of double-crossover integration strategy for replacing PfHO gene with cDNA encoding PfHO (Toxoplasma gondii codon bias) and RNA aptamers at the 5’ and 3’ ends of the gene. 580 bp sequence in 3’ UTR of PfHO (green line) was used as a probe to test for integration by Southern blot, and enzyme digestion sites with expected sizes marked on locus and plasmid. Primers used to probe integration by genome PCR are marked on wildtype and tagged loci. B) Genome PCR of parasite DNA harvested from wildtype parental, polyclonal PfHO-aptamer/TetR-DOZI, and select PfHO-aptamer/TetR-DOZI clonal cultures. C) Southern blot of digested parasite DNA harvested from wildtype, polyclonal PfHO-aptamer/TetR-DOZI, and select clonal PfHO-aptamer/TetR-DOZI cultures. D) Complete PfHO cDNA sequence used to replace the endogenous PfHO gene (identical encoded protein sequence).

Validation of custom PfHO antibody specificity. Western blot specificity tests of custom PfHO rabbit antibody in parasite lysates. A) Lysates from asynchronous or synchronous parasite cultures stained with 1:1,000 dilution of rabbit serum prior to inoculation with PfHO protein antigen. B) Lysates from E. coli expressing PfHO84–305 and from equal numbers of synchronous 3D7 parasites stained with 1:1,000 dilution of crude serum from the final bleed of a rabbit inoculated with PfHO protein antigen. Both blots were stained with an anti-rabbit HRP secondary antibody and visualized by chemiluminescence.

A) Quantitative PCR of PfHO expression ±aTC with 200 µM IPP. Transcript levels of endogenous PfHO from PfHO-aptamer/TetR-DOZI parasites grown 2 or 4 days ±aTC with 200 µM IPP. PfHO transcript abundance is normalized to the average abundance of nuclear-encoded I5P (Pf3D7_0802500), ADSL (Pf3D7_0206700), and STL (Pf3D7_0717700) transcripts, and +aTC is normalized to 100%. Normalized ratios and error bars are the average ±SD of biological triplicates. B) Additional western blot of Dd2 parasites tagged with PfHO-Aptamer/TetR-DOZI grown ±aTC + 200 µM IPP for 7 days, stained with rabbit anti-Ef1α and custom rabbit anti-PfHO primary antibodies and anti-rabbit-IRDye800 secondary antibody. Stained membrane was visualized on a Licor Odyssey CLx imager. Densitometry of western blot bands indicated >85% reduction of PfHO expression.

Giemsa-stained smears of PfHO-aptamer/TetR-DOZI parasites grown in ±aTC. Times indicated are hours post-synchronization. Grey scale bars in bottom right of images mark 5 µm.

Additional live-parasite fluorescence microscopy images of apicoplast morphology after PfHO knockdown. PfHO-aptamer/TetR-DOZI parasites episomally expressing apicoplast-localized GFP were grown 5 days ±aTC with 200 µM IPP and stained with 10 nM Hoechst.

Uncropped western blots of parasites endogenously expressing PfHO-GFP-DHFRDD that were untreated or Dox/IPP-treated for 5 days. Membrane was stained with goat anti-GFP and custom rabbit anti-PfHO primary antibodies then anti-goat-IRDye800 and anti-rabbit IRDye680 secondary antibodies. Stained membrane was visualized on a Licor Odyssey CLx imager.

Uncropped western blots of Dd2 parasites tagged with PfHO-Aptamer/TetR-DOZI grown in +aTC or -aTC/IPP conditions for 7 days, stained with rabbit anti-Ef1α and custom rabbit anti-PfHO primary antibodies and anti-rabbit-IRDye800 secondary antibody (top) 100 µg clarified lysates from parasites grown in +aTC or -aTC/IPP conditions for 3 days, run in duplicate, and stained with either rabbit anti-HSP60 or custom rabbit anti-PfHO antibodies and anti-rabbit-IRDye800 secondary antibody (bottom). Stained membranes were visualized on a Licor Odyssey CLx imager.

Uncropped Southern blot of digested parasite DNA harvested from wildtype, polyclonal PfHO-GFP-DHFRDD, and select clonal PfHO-GFP-DHFRDD cultures.

Uncropped PCR gel of parasite DNA harvested from wildtype parental, polyclonal PfHO-HA2-glmS, and select PfHO-HA2-glmS clonal cultures.

Uncropped PCR gel of parasite DNA harvested from wildtype parental, polyclonal PfHO-aptamer/TetR-DOZI, and select PfHO-aptamer/TetR-DOZI clonal cultures.

Uncropped Southern blot of digested parasite DNA harvested from wildtype, polyclonal PfHO-aptamer/TetR-DOZI, and select clonal PfHO-aptamer/TetR-DOZI cultures.

Uncropped western blot of lysates from asynchronous or synchronous 3D7 parasites stained with 1:1,000 dilution of rabbit serum prior to inoculation with PfHO protein antigen.

Uncropped western blot of lysates from E. coli expressing PfHO84–305 and from equal numbers of synchronous 3D7 parasites stained with 1:1,000 dilution of crude serum from the final bleed of a rabbit inoculated with PfHO protein antigen.

Peptide coverage of PfHO sequence detected by mass spectrometry. A) Red residues correspond to sequence detected by tryptic digest and tandem mass spectrometry after PfHO isolation from parasites. B) List of individual peptides detected within PfHO N-term and HO-domains.

qPCR analysis of PfHO knockdown in PfHO-aptamer/TetR-DOZI parasites complemented with indicated episomes. Transcript levels of endogenous PfHO from PfHO-Aptamer/TetR-DOZI + episomal complement parasites grown in ±aTC with 200 µM IPP for 5 days. PfHO transcript abundance was normalized to the average abundance of nuclear-encoded I5P (Pf3D7_0802500), ADSL (Pf3D7_0206700), and STL (Pf3D7_0717700) transcripts, and +aTC was normalized to 100%. Normalized ratios and error bars are the average ±SD of biological triplicates.

Western blot of PfHO-aptamer/TetR-DOZI parasites complemented with episomes expressing PfHO-GFP, ACPL-HO-GFP, or PfHO N-Term-GFP. Membrane was stained with goat anti-GFP primary and anti-goat-HRP secondary antibodies and visualized by chemiluminescence. PfHO-GFP parasites were grown in ±aTC with 200 µM IPP for 5 days prior to lysis.

Sequence alignment of alveolate HO-like proteins in Plasmodium falciparum, Theileria orientalis, Babesia microti, Chromera velia, and Vitrella brassicaformis. Secondary α-helical structure, predicted by AlphaFold structural models, is underlined. Alveolate-specific N-terminal α-helix is underlined in pink.

Uncropped western blots of parasites endogenously expressing PfHO-HA2 and lysates from E. coli expressing PfHO HO-like domain (PfHO84–305–HA2) stained with rat anti-HA and custom rabbit anti-PfHO primary antibodies and anti-rabbit-IRDye680 and anti-rat-IRDye800 secondary antibodies then visualized with Licor Odyssey CLx imager. Indicated molecular masses were estimated using Licor Image Studio software based on migration of the protein standards.

Uncropped western blot of PfHO-aptamer/TetR-DOZI parasites complemented with episomes expressing PfHO-GFP, ACPL-HO-GFP, or PfHO N-Term-GFP. Membrane was stained with goat anti-GFP primary and anti-goat-HRP secondary antibodies and visualized by chemiluminescence.

Spectral counts for proteins that co-purified with PfHO and not with either mitochondrial control in both IP/MS experiments. PfHO is marked in red, and proteins predicted to be apicoplast localized based on prior IP/MS studies are marked in green. The full list of proteins is provided in Figure 5 – source data 1.

List of apicoplast-localized proteins co-purified with PfHO in two IP/MS experiments ordered by average of spectral counts for each experiment.

Functional pathway predictions for the 65 apicoplast-localized proteins that co-purified with PfHO in two IP/MS experiments.

Quantification of parasite DNA fragment size by Agilent Bioanalyzer DNA analysis after pulse-sonication shearing of parasite lysates. Our DNA shearing method produced two populations of DNA fragment sizes, 1100-1600 bp and 150-300 bp.

Steady-state PCR amplification of additional nuclear (mACP: Pf3D7_1208300) and apicoplast (ClpM: Pf3D7_API03600) genes from DNA co-purified with indicated PfHO constructs.

Additional ChIP experiments in parasites with GFP-tagged PfHO constructs. (A) Relative abundance of apicoplast-encoded genes in DNA co-purified with the indicated GFP-tagged PfHO constructs by ChIP normalized to PfHO-GFP. B) Distribution and orientation of target genes on 35 kb apicoplast genome. Orange lines mark the location of the ∼100bp qPCR amplicon for each gene. C) Relative abundance of apicoplast-encoded genes in DNA co-purified with PfHO-GFP by ChIP normalized to input. D) Steady-state PCR amplification of nuclear-encoded (aACP: Pf3D7_0208500, GGPPS: Pf3D7_1128400) and apicoplast-encoded (SufB: Pf3D7_API04700, ClpM: Pf3D7_API03600) genes from DNA co-purified with full-length PfHO-GFP by ChIP ±PFA crosslinking.

RT-qPCR data for additional apicoplast genes. Transcript levels of apicoplast, nuclear, and mitochondrial genes were assessed in PfHO-Aptamer/TetR-DOZI parasites grown in ±aTC with 200 µM IPP for 3 days (84 hours post-synchronization). Each transcript is normalized to average of two nuclear transcripts – I5P (Pf3D7_0802500) and ADSL (Pf3D7_0206700). As an additional control, mitochondrial-encoded CytB (Pf3D7_MIT02300) transcript abundance is also measured relative to nuclear controls. Error bars represent average ±SD of independent biological triplicates.

Representative time-course showing ClpM: nuclear transcript levels at indicated times during first and second cycle of parasite growth ±aTC in the presence of IPP. Large error bars between independent replicate experiments are due to large variance in total ClpM expression. Within each experiment, ClpM transcripts are >10-fold less abundant in -aTC parasites at cycle 2 time-points 72, 84, and 90 hours. Error bars represent average ±SD of independent biological triplicates.

Uncropped PCR gel of nuclear-encoded and apicoplast-encoded genes from DNA co-purified with GFP-tagged PfHO constructs. Input (“I”) is total parasite DNA collected after parasite lysis and sonication, and ChIP (“C”) is DNA eluted from αGFP IP.

Uncropped PCR gel of nuclear-encoded and apicoplast-encoded genes from DNA co-purified with GFP-tagged PfHO constructs ±crosslinking.