Abstract
The biosynthesis of cAMP by mammalian membrane-bound adenylyl cyclases (mACs) is predominantly regulated by G-protein-coupled-receptors (GPCRs). Up to now the two hexahelical transmembrane domains of mACs were considered to fix the enzyme to membranes. Here we show that the transmembrane domains serve in addition as signal receptors and transmitters of lipid signals that control Gsα-stimulated mAC activities. We identify aliphatic fatty acids and anandamide as receptor ligands of mAC isoforms 1 to 7 and 9. The ligands enhance (mAC isoforms 2, 3, 7, and 9) or attenuate (isoforms 1, 4, 5, and 6) Gsα-stimulated mAC activities in vitro and in vivo. Substitution of the stimulatory membrane receptor of mAC3 by the inhibitory receptor of mAC5 results in a ligand inhibited mAC5-mAC3 chimera. Thus, we discovered a new class of membrane receptors in which two signaling modalities are at a crossing, direct tonic lipid and indirect phasic GPCR-Gsα signaling regulating the biosynthesis of cAMP.
Introduction
The structure of the first second messenger, cyclic 3‘,5‘-adenosine monophosphate (cAMP), was reported in 1958 by Sutherland and Rall. It was generated by incubation of a liver extract with the first messengers adrenaline or glucagon (1). Since then, cAMP has been demonstrated to be an almost universal second messenger used to translate various extracellular stimuli into a uniform intracellular chemical signal (2, 3). The biosynthetic enzymes for cAMP from ATP, adenylyl cyclases (ACs), have been biochemically investigated in bacteria and eukaryotic cells (4–6). Finally in 1989, the first mammalian AC was sequenced (7). The protein displayed two similar catalytic domains (C1 and C2) and two dissimilar hexahelical membrane anchors (TM1 and TM2). The latter were proposed to possess a channel or transporter-like function, properties, which were never confirmed (7). The domain architecture, TM1-C1-TM2-C2, clearly indicated a pseudoheterodimeric protein composed of two concatenated bacterial precursor proteins (8). To date, sequencing has identified nine mammalian membrane-delimited AC isoforms (mACs) with identical domain architectures (2). In all nine isoforms the catalytic domains are conserved and share extensive sequence and structural similarities which indicate a similar biosynthetic mechanism (5, 6, 9, 10). On the other hand, the associated two membrane domains differ substantially within each isoform and between all nine mAC isoforms (11–13). Bioinformatic studies, however, revealed that the membrane domains, TM1 as well as TM2, are highly conserved in an isoform-specific manner for about 0.5 billion years of evolution (11, 12).
Extensive studies on the regulation of the nine mAC isoforms revealed that the Gsα subunit of the trimeric G-proteins activate cAMP formation. Gsα is released intracellularly upon stimulation of G-protein-coupled receptors (GPCR), i.e. the receptor function for mAC regulation was assigned to the diversity of GPCRs which presently are most prominent drug targets. In 1995 Tang and Gilman reported that Gsα regulation of mammalian mACs does not require the presence of the membrane anchors. A soluble C1-C2 dimer devoid of the membrane domains was fully activated by Gsα (13). This reinforced the view that the mAC membrane anchors which comprise up to 40 % of the protein were just that and otherwise functionally inert. The theoretical possibility of mACs to be regulated directly, bypassing the GPCRs, was dismissed (14).
We were intrigued by the exceptional evolutionary conservation of the mAC membrane anchors for 0.5 billion years (11, 12). In addition, we identified a cyclase-transducing-element that connects the TM1 and TM2 domains to the attached C1 or C2 catalytic domains. These transducer elements are similarly conserved in a strictly isoform-specific manner (11, 15). Furthermore, cryo-EM structures of mAC holoenzymes clearly revealed that the two membrane domains, TM1 and TM2, collapse into a tight dodecahelical complex resembling membrane receptors (16–18). Lastly, we created a chimeric model involving the hexahelical quorum-sensing receptor from Vibrio cholerae which has a known aliphatic lipid ligand and the mAC 2 isoform. We observed that the ligand directly affected, i.e., attenuated the Gsα activation of mAC2 (19). As a proof-of concept this demonstrated that the cytosolic catalytic AC dimer serves as a receiver for extracellular signals transmitted through the dyad-related membrane anchor. Accordingly, we proposed a general model of mAC regulation in which the extent of the indirect mAC activation via the GPCR/Gsα axis is under direct ligand control via the mAC membrane anchor (19).
Here we report the results of a rigorous search for potential ligands of the mAC membrane domains. We identified aliphatic lipids as ligands for mAC isoforms 1 to 7 and 9. Isoform-dependently, the ligands either attenuate or enhance Gsα-activation in vitro and in vivo demonstrating a receptor function of the mAC proteins. The receptor properties are transferable as demonstrated by interchanging the membrane anchors between mAC3 and 5. Thus, the results define a new class of membrane receptors and establish a completely new level of regulation of cAMP biosynthesis in mammals in which tonic and phasic signaling processes intersect in a central signaling system, which is the target of frequently used drugs.
Results
Oleic acid enhances Gsα-stimulated mAC3, but not mAC5 activity
We expected lipids as ligands (19–22). We used bovine lung as a starting material because lipids are important for lung development and function (23, 24). Lipids were extracted from a cleared lung homogenate, acidified to pH 1, with dichloromethane/methanol (2:1). The dried organic phase was chromatographed on silica gel (employing vacuum-liquid-chromatography; Si-VLC) and fractions A to Q were assayed (fractionation scheme in appendix 1). Fraction E enhanced mAC3 activity stimulated by 300 nM Gsα four-fold, whereas mAC5 activity was unaffected (Fig.1A). The non-maximal concentration of 300 nM Gsα was used because it enabled us to observe stimulatory as well as inhibitory effects.
Fraction E was further separated by RP-HPLC into five subfractions (E1 – E5; appendix 1 Fig. 1). The mAC3 enhancing constituents appeared in fraction E2. It enhanced Gsα-stimulated mAC3 four-fold but had no effect on mAC5 (Fig. 1B). 1H and 13C-NMR spectra of fraction E2 indicated the presence of aliphatic lipids (appendix 1 Fig. 2). Subsequent GC/MS analysis identified palmitic, stearic, oleic and myristic acid in E2 (Fig. 1C). Concentration-response curves were established for these fatty acids with mAC3 and mAC5 stimulated by 300 nM Gsα (Fig. 1D). 20 µM oleic acid enhanced Gsα-stimulated mAC3 activity three-fold (EC50 = 10.4 µM) and 20 µM palmitic acid two-fold (EC50 = 6.4 µM), while stearic or myristic acid had no significant effect. None of these fatty acids affected mAC5 activity (Fig. 1D).
The action of oleic acid on mAC3 was instantaneous and linear for >25 min (appendix 2, Fig. 1). The Km of mAC3 for ATP (335 µM) was unaffected. Vmax was increased from 0.62 to nmol cAMP/mg/min (appendix 2, Fig. 2). Oleic acid did not affect the activity of a soluble, Gsα stimulated construct formerly used for generating a C1 and C2 catalytic dimer from mAC1 and 2, ruling out spurious detergent effects (13) (appendix 2, Fig. 3). The effect of oleic acid was further evaluated by Gsα concentration response curves of mAC3 and mAC5 in presence and absence of 20 µM oleic acid (Fig. 2, left and center). For mAC3 the calculated EC50 of Gsα in presence and absence of oleic acid were 549 and 471 nM, respectively (not significant). Over the Gsα concentration range tested with mAC3 the enhancement of cAMP formation by 20 µM oleic was uniformly about 3.4-fold (Fig. 2 right). In the case of mAC5, Gsα stimulation was not enhanced by oleic acid (Fig. 2 center and right).
To explore the ligand space, we tested 18 aliphatic C12 to C20 lipids (Table 1; structures in appendix 2 Fig. 4). At 20 µM, elaidic, cis-vaccenic and linoleic acids were efficient enhancers of Gsα-stimulated mAC3 activity. Palmitic, palmitoleic, linolenic, eicosa-pentaenoic acids and oleamide were less efficacious; other compounds were inactive (Fig. 3). Notably, the saturated C18 stearic acid was inactive here and throughout, albeit otherwise variations in chain length, and the number, location, and conformation of double bonds were tolerated to some extent, e.g., cis-vaccenic, linoleic and linolenic acids. The relaxed ligand specificity was anticipated as aliphatic fatty acids are highly bendable and bind to a flexible dodecahelical protein dimer embedded in a fluid lipid membrane. The ligand space of mAC3 somewhat resembled the fuzzy and overlapping ligand specificities of the free-fatty-acid receptors 1 and 4 (25–27).
Next, the effect of oleic acid was probed in vivo using HEK293 cells permanently transfected with mAC3 (HEK-mAC3) or mAC5 (HEK-mAC5). Intracellular cAMP formation via Gsα was triggered via stimulation of the endogenous ß-receptor with 2.5 µM of the β-agonist isoproterenol, a submaximal concentration (28). Addition of oleic acid enhanced cAMP formation in HEK-mAC3 1.5-fold (Fig. 4). Stearic acid was inactive. Under identical conditions, cAMP formation in HEK-mAC5 cells was unaffected (Fig. 4). The EC50 of oleic acid in HEK293-mAC3 cells was 0.5 µM, i.e., the potency appeared to be increased compared to respective membrane preparations whereas the efficiency was reduced, possibly reflecting the regulatory interplay within the cell. To exclude experimental artifacts, transfection efficiencies were tested by PCR. mAC3 and mAC5 transfections were similar (appendix 2; Fig. 5). Taken together, the results suggest that the enhancement of Gsα-stimulated mAC3 by oleic acid might be due to binding of oleic acid to or into an mAC3 membrane receptor (11, 20).
Oleic acid enhances Gsα-stimulated mAC 2, 7, and 9 activities
Next, we examined other AC isoforms with oleic acid as a ligand. 20 µM oleic acid significantly enhanced Gsα-stimulated activities of isoforms 2, 7, and 9, mAC1 was slightly attenuated, and isoforms 4, 5, 6, and 8 were unaffected (Fig. 5A).
Concentration-response curves were carried out for mACs 2, 7, and 9 (Fig. 5B). The EC50 of oleic acid were 8.6, 6.7 and 7.8 µM, respectively, comparable to that determined for mAC3. Exploration of the ligand space for mACs 2, 7 and 9 with the panel of 18 aliphatic lipids uncovered more active lipids (Fig. 5C). In the case of mAC2, 20 µM cis-vaccenic acid doubled cAMP formation (EC50 10.6 µM) while other compounds were inactive (Fig. 5C and for additional concentration-response curves see appendix 2 Fig. 6). For mAC7 the EC50 of elaidic was 9.7 µM (concentration-response curve see appendix 2 Fig. 7). The range of potential ligands for mAC9 was more comprehensive: 3-4-fold enhancement was observed with 20 µM palmitoleic, oleic, elaidic, cis-vaccenic, and linoleic acid. With 20 µM myristic, palmitic, palmitoleic, linolenic, and arachidonic acid 1.5-2 fold enhancements were observed (Fig. 5C and for concentration response curves see appendix 2 Fig.s 8 and 9).
Arachidonic acid and anandamide inhibit Gsα-stimulated activities of mAC1, 4, 5 and 6
Testing the panel of lipids at 20 µM with mAC isoforms 1, 4, 5, 6, and 8 we found that isoforms 1 and 4 were significantly attenuated by arachidonic acid, and somewhat less by palmitoleic acid. Other lipids had no effect (for bar plots and dose response curves see appendix 2 Fig. 10-12). Of note, eicosa-pentaenoic acid which resembles arachidonic acid but for an additional cis-Δ-17 double bond had no effect on mAC activities (appendix 2 Fig. 10, 11). Concentration-response curves for arachidonic acid with 300 nM Gsα-stimulated mAC1 and 4 yielded IC50 of 23 and 36 µM, respectively, i.e., about two-fold higher compared to the EC50 of enhancing ligands (Fig. 6A).
Next, we examined whether arachidonic acid attenuates mAC1 and 4 in intact HEK 293 cells. Surprisingly, cAMP formation in HEK-mAC1 cells stimulated by 10 µM isoproterenol was attenuated by arachidonic acid with high potency (IC50 = 250 pM), i.e., with higher potency compared to data with membranes prepared from the same cell line. In contrast, mAC4 activity examined under identical conditions was not attenuated (Fig. 6B). Currently, we are unable to rationalize these discrepancies. Possibly, mAC4 has another, more specific lipid ligand which is needed in in vivo. In general, the enhancing and attenuating effects bolster the hypothesis of specific receptor-ligand interactions and divergent intrinsic activities for different ligands.
At this point we were lacking ligands for mACs 5, 6, and 8 (appendix 2 Fig. 13-15). Possibly, the negative charge of the fatty acid headgroups might impair receptor interactions. A neutral lipid neurotransmitter closely related to arachidonic acid is arachidonoylethanolamide (anandamide) (29). Indeed, anandamide attenuated 300 nM Gsα-stimulation of mAC5 and 6 with IC50 of 42 and 23 µM, respectively, i.e., comparable to the effect of arachidonic acid on mACs 1 and 4, and distinctly less potently than the ligands for mAC 2, 3, 7, and 9 (Fig. 7A). mACs 5 and 6 may thus represent new targets for anandamide which is part of a widespread neuromodulatory system (30). The concentrations of arachidonic acid and anandamide required may be achieved in vivo by local biosynthesis and degradation. An interfacial membrane-embedded phosphodiesterase cleaves the phosphodiester bond of the membrane lipid N-arachidonoyl-ethanolamine-glycerophosphate releasing anandamide into the extracellular space (29, 31, 32). The lipophilicity and lack of charge should enable it to diffuse readily. Whether the mACs and this biosynthetic phosphodiesterase colocalize or associate with its target mACs is unknown. Degradation of anandamide is by a membrane-bound amidase, generating arachidonic acid and ethanolamine (33). Therefore, we examined whether anandamide at higher concentrations might also affect mAC1 and 4. In fact, anandamide significantly attenuated Gsα-stimulated mAC1, but distinctly not mAC4 (Fig. 7B). The IC50 of anandamide for mAC1 was 29 µM.
We also tested whether anandamide attenuated cAMP formation in vivo using HEK-mAC5 cells primed by 2.5 µM isoproterenol (Fig. 7C). 100 µM Anandamide attenuated cAMP formation by only 23 % in HEK293-mAC5 cells, the effect was significant (P < 0.01). At this point, we were unable to identify a ligand for mAC8, presumably another lipid.
Receptor properties are exchangeable between mAC isoforms 3 and 5
To unequivocally validate specific mAC-ligand-receptor interactions and regulation we generated a chimera in which the enhancing membrane domains of mAC3, i.e., mAC3-TM1 and TM2, were substituted by those of mAC5 (design in Fig. 8A, right). The intention was to obtain a chimera, mAC5(membr)-AC3(cat), with a loss of receptor function, i.e., no enhancement by oleic acid, and a gain of another receptor function, i.e., attenuation of activity by anandamide. Successful expression and membrane insertion of the chimera in HEK293 cells was demonstrated by specific conjugation to Cy5.5 fluorophore, using the protein ligase connectase (Fig. 8A, left (34)). cAMP synthesis of isolated membranes from these cells was stimulated up to 10-fold by addition of 300 nM Gsα, comparable to membranes with recombinant mAC3 or mAC5 proteins (Fig. 8B). mAC activity in the mAC5(membr)-AC3(cat) chimera was not enhanced by oleic acid, i.e., loss of receptor function, but was attenuated by anandamide, i.e., gain of receptor function (Fig. 8C and D). The attenuation was comparable to results obtained with mAC5 membranes, i.e., IC50 was 29 µM mAC5(membr)-AC3(cat) (Fig. 8E) compared to 42 µM for mAC5. This means that the attenuating receptor property of mAC5 was successfully grafted onto the mAC3-catalytic dimer. We take this to support the hypothesis that the mammalian mAC membrane domains operate as receptors using lipid ligands. The data virtually rule out unspecific lipid effects such as disturbance of membrane integrity by intercalation and surfactant or detergent effects. In addition, the data demonstrated that the signal most likely originates from the receptor entity and is transmitted through the subsequent linker regions to the catalytic dimer.
The findings were further substantiated in vivo using HEK293-mAC5(membr)-AC3(cat) cells. cAMP formation primed by 2.5 µM isoproterenol was attenuated by anandamide in HEK293-mAC5(membr)-AC3(cat) cells by 66%, (Fig. 9), by far exceeding the anandamide attenuation in HEK293-mAC5 cells of 23 %. In HEK293-mAC5(membr)-AC3(cat) cells oleic acid was ineffective, i.e., loss of function (data not shown). The results bolster the notion that mAC isoforms are receptors with lipids as ligands.
Lastly, we prepared membranes from mouse brain cortex in which predominantly mAC isoforms 2, 3 and 9 are expressed, isoforms with demonstrated enhancement of Gsα stimulation by oleic acid (35). In cortical membranes 20 µM oleic acid enhanced Gsα stimulated cAMP formation 1.5-fold with an EC50 of 5 μM, almost identical to the one determined for mAC2, 3, 7 and 9 (Fig. 10). This suggests that mACs in brain cortical membranes are similarly affected by fatty acids.
Discussion
In the past, the biology of the two membrane anchors of mACs, highly conserved in an isoform-specific manner, remained unresolved. The theoretical possibility of a receptor function of these large hexahelical anchor domains, each comprising 150-170 amino acids, was considered unlikely (14). Our data are a transformative step toward resolving this issue and introduce lipids as critical participants in regulating cAMP biosynthesis in mammals. The first salient discovery is the identification of the membrane domains of mACs as a new class of receptors for chemically defined ligands which set the level of stimulation by the GPCR/Gsα system. This conclusion is based on (i) the dodecahelical membrane domains of the nine mAC receptors have distinct, conserved isoform-specific sequences for the TM1 and TM2 domains (11); (ii) the receptors have distinct ligand specificities; (iii) isoform dependently ligands either enhance or attenuate Gsα-stimulated mAC activities; (iv) receptor properties are transferable between isoforms by interchanging membrane domains; (v) isoproterenol-stimulated formation of cAMP in vivo is affected by addition of extracellular ligands. (vi) Gsα-stimulated cAMP formation in mouse cortical membranes is enhanced by oleic acid. Therefore, the results establish a new class of receptors, the membrane domains of mACs, with lipids as ligands. The data question the utility of the currently used mAC sub-classification, which groups mAC1, 3, 8, mAC2, 4, 7, mACs 5, 6, and mAC9 together (2). At this point, mAC 1, 4, 5, and 6 which are ligand-attenuated, may be grouped together and a second group may consist of isoforms 2, 3, 7, and 9 which are ligand-enhanced. Our data do not contradict earlier findings concerning regulation of mACs via GPCRs, cellular localization of mAC isoforms or regional cAMP signaling (2). Instead, the data reveal a completely new level of regulation of cAMP biosynthesis in which two independent modalities of signaling, i.e., direct, tonic lipid signaling and indirect phasic signaling via the GPCR/Gsα circuits intersect at the crucial biosynthetic step mediated by the nine mAC isoforms.
The second important finding is the observation that the extent of enhancement of mAC3 activity by 20 µM oleic acid is uniform up to 1000 nM Gsα (Fig. 2 right). We suppose that in mAC3 the equilibrium of two differing ground states favors a Gsα unresponsive state and the effector oleic acid concentration-dependently shifts this equilibrium to a Gsα responsive state (19). In contrast, the equilibrium of ground states of mAC5 probably is opposite, i.e. the one accessible to Gsα stimulation predominates and stimulation by Gsα is maximal. In addition, oleic acid has little effect because the mAC5 receptor domain does not bind oleic acid (Fig. 1D, right, and Fig. 2, center). A ligand for mAC5, e.g., anandamide or arachidonic acid, likely shifts the equilibrium of ground states to a Gsα unresponsive state and inhibits stimulation. The biological balance of ground states appears to be an intrinsic property which is isoform-specifically imprinted in mACs. Probably, it defines a major element of regulation and enables distinct inhibitory or stimulatory inputs by extracellular lipid ligands. The ground states probably are separated by a low transition energy and are stabilized by receptor occupancy. Hitherto available structures required Gsα and/or forskolin for stabilization and probably did not capture different ground states (14, 16, 17, 36–38). Mechanistically, tonic levels of lipid ligands affect the ground states and thus set the bounds of cAMP formation elicited by phasic GPCR/Gsα-stimulation. As such lipid signaling through the mAC membrane receptors appears to represent a higher level of a systemic regulatory input based on constant monitoring the physiological and nutritional status of an organism.
Lipid signaling is much less characterized than solute signaling (39). Most of the highly functionalized ligands for GPCRs are storable in vesicles and the release, inactivation and removal are strictly controlled. On the other hand, the very nature of lipids, i.e., high flexibility of aliphatic chains, low water solubility, propensity for nonspecific protein binding, membrane permeability and potential effects on membrane fluidity complicate discrimination between extra- and intracellular lipid actions (26). Yet, viewed from an evolutionary perspective, lipids possibly are ideal primordial signaling molecules because for the emergence of the first cell lipids were required to separate an intra- and extracellular space. Conceivably, lipids derived from membrane lipids were used for regulatory purposes early-on. In association with the evolution of bacterial mAC progenitors lipid ligands may have persisted in evolution and regulation by GPCR/Gsα in metazoans was acquired and expanded later.
The concentrations of free fatty acids in serum or interstitial fluid usually are rather low (27, 40, 41). This raises the question of the origin of lipid ligands. One possibility is that the lipid ligands are acutely extracted from membrane lipids by membrane-integral hydrolases as known for anandamide and arachidonic acid (32, 42). Additional potential lipid sources usable for ligand generation may be, among others, exosomes, serum lipids, chylomicrons, blood triglycerides and even lipids possibly originating from the microbiome. The discovery of lipids as ligands for the mAC receptors broadens the basis of regulation of cAMP biosynthesis with potentially wide-ranging consequences in health and disease.
Materials and methods
Reagents and materials
ATP, creatine kinase, and creatine phosphate were from Merck. Except for lauric acid (Henkel) and 1,18-Octadecanedicarboxylic acid (Thermofisher Scientific), lipids were from Merck. Lipid stock solutions were prepared in DMSO and kept under nitrogen. The DMSO concentrations in in vitro and in vivo assays were maximally 1%, a concentration without any biochemical effect. The constitutively active GsαQ227L mutant was expressed and purified as described earlier (43–45).
General Experimental Procedures
For HPLC analysis, a Waters HPLC system (1525 pump, 2996 photodiode array detector, 7725i injector, 200 series PerkinElmer vacuum degasser) was used. Solvents were HPLC or LC-MS grade from Merck-Sigma. One-dimensional 1H and 13C NMR spectra were recorded on a 400 MHz Bruker AVANCE III NMR spectrometer equipped with a 5 mm broadband SmartProbe and AVANCE III HD Nanobay console. Spectra were recorded in methanol-d4 and calibrated to the residual solvent signal (δH 3.31 and δC 49.15 ppm).
Lung tissue extraction and fractionation
1.24 kg bovine lung was minced in a meat grinder, then mixed and homogenized with 1.2 L 50mM MOPS, pH 7.5, in a Waring blender (4°C) resulting in 2.3 L homogenate. It was centrifuged (30 min, 4°C, 7200×g) resulting in 1.2 L supernatant. The pH was adjusted to 1 using 7% HCl. Equal volumes of CH2Cl2/MeOH (2:1) were mixed with the supernatant in a separatory funnel and shaken vigorously. Centrifugation was at 5300×g for 30 min. The lower organic CH2Cl2 layer was recovered and the solvent was evaporated yielding 2 g of dried material. It was dissolved in 100 ml petroleum ether and subjected to normal-phase silica gel vacuum liquid chromatography (60 H Supelco). The column was eluted stepwise with solvents of increasing polarity from 90:10 petroleum ether/EtOAc to 100% EtOAc, followed by 100% MeOH. 17 fractions (A-Q) of 300 mL were collected and dried. Fraction E (eluting at 40:60 petroleum ether/EtOAc) was analysed by RP-HPLC using a linear MeOH/H2O gradient from 80:20 to 100:0 (0.1% TFA) for 15 min, followed by 100:0 for 30 min (Knauer Eurosphere II C18P 100-5, 250 x 8 mm, 1.2 mL/min flow rate, UV-absorbance monitored at 210 nm) to yield five subfractions; E1-E5. Fraction E2 was analysed by 1H- and 13C-NMR which indicated the presence of aliphatic lipids and fatty acids (Fig. S2).
GC-MS analysis
Fraction E2 was analysed by GC-MS. Acids were acid trimethylsilylated using N,O-bis(trimethylsilyl)trifluoroacetamide + trimethylchlorosilane (BSTFA + TMCS, 99:1 v/v). The mixture was heated for 2 h at 90°C. After cooling and clearing the sample was transferred into a GC vial in 200 µL hexane.
An Agilent Technologies GC system (8890 gas chromatograph and 5977B mass spectrometer equipped with a DB-HP5MS UI column, 30 m x 0.25 mm, film thickness of 0.25 µm) was used. Injection volume was 1 µL. The temperature was kept at 100 C for 5 min, and then increased at 53°C/min to 240 C. The rate was decreased to 3 C/min to reach 305 C. Carrier gas was He2 (99.9%; 1.2 mL/min). Ionization was with 70 eV and MS spectra were recorded for a mass range m/z 35-800 for 35 min. Compounds were identified by comparing the spectra with those in the NIST library. Individual compound content is given as a relative % of the total peak area.
Plasmid construction and protein expression
hAC sequences were from NCBI were: ADCY1: NM_021116.3; ADCY2: NM_020546.2; ADCY3: NM_004036.4; ADCY4: NM_001198568.2; ADCY5: NM_183357.2; ADCY6: NM_015270.4; ADCY7: NM_001114.4; ADCY8: NM_001115.2; ADCY9: NM_001116.3.
Human mAC genes were from GenScript and fitted with a C-terminal FLAG-tag. The chimera mAC5(TM)_mAC3(cat) had an N-terminal connectase-tag, MPGAFDADPLVVEIAAAGA, followed by AC5(1–402)_AC3(250–631)_AC5(761–1009)_AC3(862–1144). The gene was synthesized by GenScript. HEK293 cells were maintained in DMEM with 10% FBS at 37°C and 5% CO2. Transfection with AC plasmids was with PolyJet (SignaGen, Frederick, MD, USA). Permanent cell lines were generated by selection for 7 days with 600 µg/mL G418 and maintained with 300 µg/mL For membrane preparation, cells were tyrpsinized, collected by centrifugation (3000xg, 5 min) and lysed and homogenized in 20 mM HEPES, pH 7.5, 1 mM EDTA, 2 mM MgCl2, 1 mM DTT, one tablet of cOmplete, EDTA-free (per 50 mL) and 250 mM sucrose by 20 strokes in a potter homogenizer on ice. Debris was removed (5 min at 1000xg, 0°C), membranes were collected at 100,000xg, 60 min at 0°C, suspended and stored at -80°C in 20 mM MOPS, pH 7.5, 0.5 mM EDTA, 2 mM MgCl2. Membrane preparation from mouse brain cortex was according to (19, 46). Three cerebral cortices were dissected and homogenized in 4.5 ml cold 48 mM Tris-HCl, pH 7.4, 12 mM MgC12, and 0.1 mM EGTA with a Polytron hand disperser (Kinematica AG, Switzerland). The homogenate was centrifuged for 15 min at 12000xg at 4°C. The pellet was washed once with 5 mL 1 mM KHCO3. The final suspension in 2 mL 1 mM KHCO3 was stored in aliquots at -80 C.
DNA extraction
DNA from 1×106 cells of permanently transfected and non-transfected HEK293 cells was extracted using the High Pure PCR Template Preparation Kit (Roche) according to the manufacturer’s instructions. DNA concentrations were determined at 260 nm using a sub-microliter cell (IMPLEN) in a P330 NanoPhotometer (IMPLEN). Elution buffer (Roche) was used for blanks.
Polymerase chain reaction
100 ng of template DNA was mixed with 0.5 µM Forward primer and 0.5 µM Reverse primer. 12.5 µL 2X KAPA2G Fast (HotStart) Genotyping Mix with dye and water was added, total reaction volume 25 µL according to the KAPA2G Fast HotStart Genotyping Mix kit (Roche) protocol. PCR followed the cycling protocol in a Biometra T3000 thermocycler:
The PCR products were directly loaded on a 1.5% agarose gel. A 1 kb DNA ladder (New England Bio Labs #N3232S) was mixed with Gel Loading Dye Purple 6X (New England Biolabs #B7024S) and water. After running the gel for 15-20 minutes at 90 V in 1X TAE buffer, the gel was stained in an Ethidium bromide bath and left running for another 10-20 minutes. The gels were then evaluated under UV light in a UVP GelStudio PLUS (Analytik Jena) gel imager.
Adenylyl cyclase assay
In a volume of 10 μl, AC activities were measured using 1 mM ATP, 2 mM MgCl2, 3 mM creatine phosphate, 60 μg/mL creatine kinase, and 50 mM MOPS pH 7.5. The cAMP assay kit from Cisbio (Codolet, France) was used for detection according to the supplier’s instructions. For each assay a cAMP standard curve was established. EC50 and IC50 values were calculated by GraphPad Prism version 8.4.3 for Windows, GraphPad Software, San Diego, California USA, www.graphpad.com.
cAMP accumulation assay
HEK293 cells stably expressing mAC isoforms 3, 5, and mAC5(memb)_mAC3(cat) were plated at 2500-10000 cells/well into 384 well plates. Cells were treated with varying concentrations of lipids and incubated for 10 min at 37°C and 5% CO2. 2.5-10 μM isoproterenol was added to stimulate cAMP formation and cells were incubated for 5 min. HEK293-AC5-3 was assayed in the presence of the phosphodiesterase inhibitor 0.5 mM isobutyl-methyl-xanthine. Addition of Cisbio HTRF detection reagents stopped the reaction and cAMP levels were determined.
Data handling and analysis
Experimental results were evaluated using GraphPad Prism version 8.4.3. Usually Graphs were generated by GraphPad and assembled in PowerPoint.
Acknowledgements
We are indebted to Prof. Andrei Lupas, Max-Planck-Institute of Biology, Tübingen, for support, advice and critique. We to thank N. Grzegorzek, Organic Chemistry, for GC/MS measurements. Funding was from the Deutsche Forschungsgemeinschaft (Schu275/45-1) and from institutional funds from the Max-Planck-Gesellschaft;
Conflict of interest
The authors declare no conflict of interest with the contents of this article. Data and Materials availability: All data are available in the paper or in the two appendices.
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