Sex transformed Drosophila larval gonads
A. Visualizing the gene expression (reads) of dsx locus on the Genome browser from the RNA-seq data of larval gonads from wild type ovary (XX), testis sex transformed into ovary (XY; traF), wild type testis (XY) and ovary sex transformed into testis (XX; tra-). The dsxF mRNA is represented by Red and dsxM mRNA is represented by blue genomic tracks to follow female and male specific splicing patterns.
XX; tra- (Df(tra)/tra1) is a progeny from w1118; Df(3L)st-j7, Ki/TM6 females and w1118/Y; tra[1]/TM6 males.
XY; traF (U2af-traF/+; tra5) is a progeny from X/X; tra[F.U2af50]/+; tra[5] females and FM7, act-gfp/Y males.
B, C, D and E are Immuno stained XX, XY; traF, XY and XX; tra- gonads to observe the morphology VAS: green (Germ cells), TJ: Magenta (cyst cells), DAPI: gray (Nuclei). Scale bar 40 μm
F, G, H and I are the Immuno stained XX, XY; traF, XY and XX; tra- gonads to observe Niche regions VAS: Green (Germ cells), Engrailed: Red (terminal filaments), Ncad: Red (hub), Scale bars 20 μm
White arrows in F and G are pointing to similar looking niche regions in XX and XY; traF.
White arrow in H is pointing no-gap between the hub to the nearest germ cells. White arrow in I is pointing the gap between the hub to the nearest germ cells.
J. Volume (V=4/3!abc) of XX, XY; traF, XY and XX; tra- larval gonads.
K. Total number of germ cells in the larval gonads of the genotype represented.
L. The distance between the somatic cells in the niche region to the nearest germ cell.
Two-sample Wilcoxon Rank Sum (Mann-Whitney) test is utilized to find the statistical significance in J - L. In L, triangles indicate numbers less than 1 were raised to 1.