CNS-wide Atlas of SPOTlight Activity in ChAT-positive neurons.
(A) Brain and spinal cord regional analysis of R:G ratio values in ChAT vs non-ChAT neighboring neurons. (B) Regional analysis of normalized Log2 (fold change) values of R:G ratios in ChAT(+)/non-ChAT neighboring neurons. (A-B) Statistical summary tables and relative intensities of fluorescent proteins for A-B available in Figure 4 – Supplement 1A-C. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. X: n = 4 mice, ChAT(+) n = 84 cells, Non-ChAT n = 66 cells; III/IV: n = 5 mice, ChAT(+) n = 144 cells, Non-ChAT n = 145 cells; Lumbar spinal cord n = 3 mice, ChAT(+) n = 250 cells, non-ChAT n = 165 cells; Cervical spinal cord n = 3 mice, ChAT(+) n = 257 cells, non-ChAT n = 182 cells; PBG: n = 3 mice, ChAT(+) n = 76 cells, Non-ChAT n = 69 cells; V: n = 6 mice, ChAT(+) n = 151 cells, Non-ChAT n = 136 cells; MH: n = 3 mice, ChAT(+) n = 241 cells, Non-ChAT n = 173 cells; Thoracic spinal cord n= 3 mice, ChAT(+) n = 342 cells, non-ChAT n = 210 cells; DS: n = 6 mice, ChAT(+) n = 338 cells, Non-ChAT n = 1154 cells; PPn: n = 5 mice, ChAT(+) n = 123 cells, Non-ChAT n = 200 cells; NDB: n = 5 mice, ChAT(+) n = 105 cells, Non-ChAT n = 144 cells; LDT: n = 5 mice, ChAT(+) n = 194 cells, Non-ChAT n = 241 cells; GPi/GPe: n = 4 mice, ChAT(+) n = 34 cells, Non-ChAT n = 77 cells; VII: n = 3 mice, ChAT(+) n = 91 cells, Non-ChAT n = 56 cells; XII: n = 4 mice, ChAT(+) n = 146 cells, Non-ChAT n = 101 cells; VI: n = 5 mice, ChAT(+) n = 52 cells, Non-ChAT n = 98 cells; NAc: n = 6 mice, ChAT(+) n = 205 cells, Non-ChAT n = 776 cells; MS: n = 4 mice, ChAT(+) n = 61 cells, Non-ChAT n = 111 cells; OT: n = 4 mice, ChAT(+) n = 39 cells, Non-ChAT n = 94 cells. (C) Representative images from select regions: Ventral horn of cervical spinal cord (VH), scale bar 50 µm; Oculomotor nucleus/trochlear nucleus (III/IV), scale bar 50 µm; Laterodorsal tegmental nucleus (LDT), scale bar 50 µm; Dorsal motor nucleus of the vagus nerve (X) and Hypoglossal nucleus (XII), scale bar 100 µm; Pedunculopontine nucleus (PPn), scale bar 50 µm. Example images for other regions are available in Figure 4 – Supplement 2.