Abstract
Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and reduced immune response, CIN generates aneuploidy-induced stresses that could be exploited for immunotherapies. Macrophages, particularly, have been understudied in the CIN context. Here, through MPS1 inhibition-induced CIN in poorly immunogenic B16F10 mouse melanoma, we find that CIN- afflicted cancer cells skew macrophages towards an anti-cancer phenotype while also pushing them away from a pro-cancer one. We confirm these findings via RNA-sequencing, protein expression, and short-term tumor studies. These results further translate to in vivo efficacy in suppressing tumor growth: Mice can survive challenges of CIN-afflicted tumors. Long-term survival, however, is dependent on CD47 expression and IgG opsonization. Mice bearing CIN- afflicted tumors with wild-type CD47 levels see prolonged survival compared to their chromosomally stable counterparts, but all succumb. Mice bearing CIN-afflicted CD47 knockout tumors, however, show 28% long-term survival. When CD47 knockout was further paired with IgG opsonization, survival rate increased to 97%. Successful rejection and clearance of CIN- afflicted tumors induced de novo anti-cancer IgG antibodies that were multi-epitope and functionally promoted macrophage-mediated phagocytosis. These de novo IgG antibodies could also suppress in vitro tumoroid and in vivo tumor growth in a CD47 knockout context. These results highlight an unexpected therapeutic benefit from CIN when paired with maximal macrophage anti-cancer activity: an anti-cancer vaccination-like antibody response that can lead to durable cures and further potentiate cell-mediated acquired immunity.
Introduction
Chromosomal instability (CIN) has long been thought to be indicative of poor prognosis and reduced immune cell activity against tumors (Davoli et al., 2017; Vasudevan et al., 2021). CIN is a state of high frequency of chromosome mis-segregation, which often generates micronuclei and can ultimately cause aneuploidy—an abnormal number of chromosomes. CIN-induced genomic heterogeneity can serve as a tumor promotor (Sheltzer et al., 2017) and allow some tumor subpopulations to favor aggression, metastatic potential, immune evasion, and resistance to therapies (Ben-David & Amon, 2019; Chunduri & Storchová, 2019; Vasudevan et al., 2021). However, early-stage CIN also induces anti-cancer vulnerabilities (Cohen-Sharir et al., 2021; Vasudevan et al., 2020), such as proliferation deficits (Wang et al., 2021). These early-stage CIN-afflicted cells have yet to adapt and achieve aneuploidies that favor growth and immune evasion (Tripathi et al., 2019, Vasudevan et al., 2021). CIN in diploid cells that is caused by spindle checkpoint disruption by MPS1 inhibition (MPS1i) further induces a senescence-associated secretory pathway phenotype and upregulation of NF-κB and interferon-mediated pathways, among others, that drive immune clearance of chromosomally aberrant cells (Santaguida et al., 2017; Wang et al., 2021). CIN and ploidy changes also inhibit tumor growth in immunocompetent mice while having little effect in immunocompromised mice (Senovilla et al., 2012; Boilève et al., 2013), suggesting CIN can somehow increase immunogenicity.
Recent analyses of The Cancer Genome Atlas (TCGA) particularly showed that highly aneuploid tumors have macrophages that are polarized toward a pro-cancer, M2-like phenotype, among other pro-cancer immune changes (Davoli et al., 2017; Taylor et al., 2018). MPS1i-treated cancer cells have also been occasionally found to escape immune-mediated clearance (Wang et al., 2021), but another study further found that cancer cells respond to CIN ultimately by triggering IL-6-STAT3 signaling (Hong et al., 2022) that protect from CIN-induced cell death, minimize interferon-related anti-cancer responses, and allow cells to adapt to CIN- and aneuploidy-induced stresses. Among these studies, two key observations stood out. First, ploidy changes tend to increase factors that can promote macrophage-mediated phagocytosis (Chao et al., 2010; Krysko et al., 2018). Second, pro-survival signaling amidst CIN leads to an increase of other factors (such as IL6) that induce a pro-cancer, M2-like phenotype (Fernando et al., 2014). Together, these observations led us to hypothesize that macrophages may play a key effector role in confronting and influencing CIN- and aneuploidy-afflicted tumors.
Macrophages have become attractive candidates for immunotherapies due to their ability to phagocytose cancer cells (Alvey et al., 2018; Klichinsky et al., 2020). Generally, phagocytosis of ‘self’ cells is inhibited by the key macrophage checkpoint interaction between SIRPα on the macrophage and CD47 on the target cell (Oldenborg et al., 2000). CD47 is ubiquitously expressed on all cells, including cancer cells (Willingham et al., 2012). While tumor cell engulfment can be driven to some extent via IgG opsonization by using anti-tumor monoclonal antibodies that bind Fc receptors on macrophages (Alvey et al., 2017; Morrissey et al., 2020; Suter et al., 2021; Tsai & Discher, 2008), this is generally insufficient to eliminate cancers, especially solid tumors. Maximal macrophage-mediated phagocytosis is achieved when CD47-SIRPα signaling is disrupted, in addition to IgG opsonization. However, achieving complete tumor rejection is still a major challenge for macrophage-oriented therapies (Ingram et al., 2017; Sockolosky et al., 2016), even after having identified these three key factors. Previous studies achieved durable cures in immunocompetent mice, with some acquired immunity benefits, but tumor rejection success is highly variable (Dooling et al., 2022; Hayes et al., 2022). Additionally, in vivo macrophages often polarize toward tumor-associated macrophage (TAM) phenotypes, which correlate with poor clinical prognoses (Cerezo-Wallis et al., 2020; Noy & Pollard, 2014). TAMs also have poor phagocytic function and promote tumor growth and invasion (Alvey et al., 2017; Georgouli et al., 2019). Nonetheless, CIN could create conditions in which macrophages are not outcompeted in number by cancer cell proliferation and avoid becoming TAM-like.
Inspired by recent advances in exploiting CIN-related anti-cancer vulnerabilities, we hypothesized that early-stage CIN in cancer cells may stimulate higher anti-cancer macrophage activity than their chromosomally stable counterparts (under conditions of maximal phagocytosis). Our results provide detailed in vitro and in vivo evidence that early-stage CIN in cancer favors anti-cancer macrophage activity and consistently leads to durable cures in mice when under conditions of maximal phagocytosis. Elimination of these CIN-afflicted solid tumors further drives development of both anti-cancer opsonizing IgGs and enhanced cell-mediated immunity, both of which help suppress growth against aggressive chromosomally stable tumors.
Results
MPS1i-induces CIN and generates conditions that favor anti-cancer macrophages while minimizing skewing towards a pro-cancer macrophage phenotype
To investigate the possible effects that CIN may have in mediating macrophage immune response, we chose to work with the poorly immunogenic B16F10 mouse melanoma model. To induce CIN in B16F10, we treated cells with the MPS1 inhibitor reversine (Hong et al., 2022; Kitajiam et al., 2022; Santaguida et al., 2010; Santaguida et al., 2017). Treatment occurred for 24 h, after which the drug was washed out, and cells were allowed to recover for an additional 48 h (Fig. 1A). After the recovery period, we quantified CIN in B16F10 cells, characterized its effects in early-stage tumors, and studied potential effects on macrophages in vitro (Fig. 1A). We first confirmed that MPS1i treatment increased the frequency of micronuclei formation in interphase cells (Fig. 1B), as micronuclei are often used as a surrogate for CIN and genome instability (Cohen-Sharier et al., 2020; Crasta et al., 2012; Harding et al., 2017; Mackenzie et al., 2017). Regardless of the MPS1i concentration used, we saw substantial increases over the cell line’s baseline level of micronuclei formation. We further verified these results with other MPS1i inhibitors AZ3146 and BAY 12-17389 (Fig. S1A) to confirm the effect was not somehow limited to just reversine. Upon confirmation of increased micronuclei induction, we then proceeded to quantitatively confirm and assess copy number variations that resulted from MPS1i treatment using single-cell RNA-sequencing. For DMSO-treated B16F10 cells, approximately 10% of the population were considered aneuploid outliers (Fig. 1C-top; S1B-C) – cells with copy number profiles that are 2.5 standard deviations away from the distribution peak (see Methods). However, 34% of the MPS1i-treated B16F10 cells were aneuploid (Fig. 1C-bottom; S1B-C).
After confirming that MPS1i indeed induces CIN and quantifiable aneuploidy in B16F10 cells, we next sought to understand how CIN may affect early-stage immune response and tumor development. We proceeded to establish tumors in mice with either MPS1i-treated or DMSO-treated B16F10 (following the same schema outlined in Fig. 1A). We isolated tumors from mice at two timepoints, five and ten days after initial challenge, and then we processed whole-tumors for bulk RNA-sequencing (Fig. 1D). Comparison of tumors comprised of MPS1i-treated and DMSO-treated cells by differential gene expression showed distinct downregulated transcripts for numerous genes encoding M2-like (pro-cancer) macrophage polarization markers (Fig. 1E). At day 5 post-challenge, we see that classical M2-like, pro-cancer markers Arg1, Marco, and Cd274 are downregulated. However, several M2 markers are upregulated at this timepoint: Mrc1, Cd163, and genes related to the complement component 1a (C1q). By day 10, Marco, Mrc1, Cd163, Cd274, and the C1q complex genes are now all downregulated. Arg1, however, returns to either basal or increased expression on day 10. Overall, though, we generally see globally decreased expression for most markers by day 10. Furthermore, cytokines Ccl2, Ccl4, Ccl7, Ccl22, and Il21r, which associated with an M2-like, pro-cancer macrophage phenotype (Cerezo-Wallis et al., 2020), were found to be consistently downregulated at both days 5 and 10 post-challenge. Gene set enrichment analysis further revealed that numerous pathways related cell cycle and proliferation were also downregulated (Fig. 1F), consistent with previous studies (Cohen-Sharir et al., 2020; Wang et al., 2021). These downregulated pathways suggest longer tumor doubling time since cells have yet to adapt to these CIN-associated proliferation deficits.
The identification of downregulated M2-like macrophage markers in CIN-afflicted tumors next led us to better assess how early-stage CIN in cancer may influence macrophages specifically. Previous studies (Cerezo-Wallis et al., 2020; Xian et al., 2021) have found that the secretome can have profound and rapid polarization effects on bone marrow-derived macrophages (BMDMs) in vitro. We proceeded to perform a similar secretome study, in which conditioned media from MPS1i-treated or DMSO-treated B16F10 cells was collected and added to previously differentiated BMDMs. BMDMs were treated with conditioned media for 24 h, after which they were collected and processed for single-cell RNA-sequencing (Fig. 1G). Unsupervised clustering analysis using uniform manifold approximation and projection (UMAP) nonlinear dimensionality reduction identified four distinct macrophage population clusters: two clusters (0 and 2) that consisted of approximately 75% of macrophages treated with MPS1i-treated B16F10 secretome and two clusters (1 and 3) that consisted of approximately 75% macrophages treated with DMSO-treated B16F10 secretome (Fig. 1H).
The gene expression profiles of these clusters showed that the two clusters (1 and 3) that consisted mostly of macrophages treated with DMSO-treated B16F10 secretome had increased expression of anti-inflammatory, M2-like pro-cancer macrophage polarization markers (Fig. 1I). Consistent with the whole-tumor bulk RNA-seq, we were successfully able to capture increased expression of Mrc1 in both clusters 1 and 3 and observed increased expression of Ccl2, Ccl4, and Ccl7 in cluster 3. More importantly though, gene expression revealed that clusters 1 and 3 tended to downregulate many genes associated with a pro-inflammatory, M1-like anti-cancer response (Fig. 1J). Clusters 0 and 2, on the other hand, which consisted mostly of MPS1i-treated B16F10 secretome, showed little-to-no expression for most M2-like, pro-cancer markers (Fig. 1I) and increased expression of pro-inflammatory, anti-cancer markers (Fig. 1J). These results suggest that early-stage CIN in cancer cells engages pro-inflammatory macrophage activity.
Macrophages polarize to an M1-like phenotype and can clear CIN-afflicted tumoroids
Next, we sought to experimentally confirm the polarization effects. Here, we use B16F10 CD47 knockout (KO) cells (Fig. S2A) to remove the inhibitory effects of CD47 on phagocytosis and more easily study macrophage engulfment ability (Fig. S2B-C). Moving forward, we chose to use a B16F10 tumoroid model to better simulate the biophysical properties of the 3D tumor microenvironment and proliferative capacity of tumor masses (Fig. 2A) (Dooling et al., 2022). We chose this model primarily because 2D phagocytosis assays fail to consider the mechanical cohesive forces between tumor cells and other biophysical aspects that can affect immune response (Nia et al., 2020). We have previously found that macrophages need to phagocytose cooperatively—large numbers of macrophages working together to overcome the mechanical strength of cohesion between solid tumor cells—to achieve durables responses (Dooling et al., 2022). CIN can also often induce additional effects on cells in its early stages, such as increases in cell size due to cytokinesis defects and poly-aneuploidy (Chunduri & Storchová, 2019; Mallin et al., 2022). Single macrophages may struggle to phagocytose these large targets in a conventional 2D assay (Champion et al., 2006), even with IgG opsonization, which we experimentally confirmed with MPS1i-treated cells (Fig. S2B). MPS1i treatment, in fact, increases cell size, as measured by flow cytometry (Fig. S2C), although size effects are confounded by decreased Tyrp1 expression, the antigen targetable for opsonization using anti-Tyrp1 (Fig. S2D). CD47 levels were relatively consistent across treatment conditions and similar to non-perturbed levels (Fig. S2E) for B16F10 sgCtrl. We thus hypothesized that cooperative phagocytosis (Dooling et al., 2022) may be able to overcome the CIN-induced size increases and reduced binding of anti-Tyrp1. BMDMs were added to pre-assembled tumoroids at a 3:1 ratio, and this “immuno-tumoroid” co-culture was maintained for five days. We first assessed surface protein expression of macrophage polarization markers. Consistent with the polarization trends in our whole-tumor bulk RNA-sequencing and single-cell RNA-sequencing of BMDMs (Fig. 1E, 1I-J), we find that BMDMs co-cultured in tumoroids of MPS1i-treated B16F10 showed increased expression of M1-like, anti-cancer macrophage markers MHCII and CD86 while having decreased expression of M2-like, pro-cancer markers CD163 and CD206 (Fig. 2B-C).
We next wondered if these polarization studies translated to functional effects on macrophage-mediated phagocytosis and clearance of tumoroids. We again prepared tumoroids, later adding BMDMs to these pre-assembled tumoroids at a 3:1 ratio with either anti-Tyrp1 or mouse IgG2a isotype control for IgG opsonization. In our 3D tumoroid in vitro assays, we found that macrophages can suppress the growth of chromosomally unstable tumoroids and clear them, surprisingly both with and without anti-Tyrp1 (Fig. 2D-E), regardless of MPS1i concentration used for treatment. In contrast, DMSO-treated B16F10 cells (chromosomally stable with regular proliferative capacity) require anti-Tyrp1 for macrophages to clear them (Fig. 2D, S3B). These results link the phenotypic studies with functional effects: CIN can induce anti-cancer macrophage phenotypes to favor macrophage-mediated clearance.
We also attempted to use two other MPS1i inhibitors to see if we achieved similar results: tumoroids made of AZ3146-treated cells, which BMDMs could only suppress at very high concentrations (Fig. S3C-i), and tumoroids made of BAY 12-17389-treated cells, which were heavily suppressed and/or cleared similarly to tumoroids comprised of reversine-treated cells (Fig. S3C-ii). Given that AZ3146-treated cells tended to proliferate well, we next sought to verify if tumoroids made of cells treated with either BAY 12-17389 or reversine would eventually grow. We observed that tumoroids decreased in size 24 h after plating, most likely due to some cell death (Fig. S3D). Afterwards though, tumoroids generally showed some proliferation, albeit with deficits. This suggests that the anti-cancer macrophage activity observed may require CIN be severe enough to induce some proliferation deficits. Otherwise, low-level CIN may be tolerable for cancer cells, eventually favor their evolution (Vasudevan et al., 2020), and allow cancer cells to still proliferate more rapidly than phagocytosis kinetics can manage.
Lastly, we sought to confirm both our transcriptomic analyses and tumoroid surface marker expression before proceeding to long-term in vivo experimentation. We proceeded to establish tumors in mice with either MPS1i-treated or DMSO-treated B16F10 cells (the drug treatment followed the same schema outlined in Fig. 1A). We then isolated tumors five days post-challenge for immune infiltrate analyses by flow cytometry (Fig. 2F, S5). We found that CIN-afflicted tumors had approximately 3-fold more CD45+ immune cells (Fig. 2G-i), 2.5-fold more CD45+ CD11b+ (myeloid) cells (Fig. 2G-ii), and a near 6-fold increase in F4/80-positive macrophage (Fig. 2G-1. iii) compared to their DMSO counterparts. Furthermore, we found that CIN-afflicted tumors showed approximately 6-fold more CD86hi CD206lo (M1-like, anti-cancer) macrophages compared to their DMSO counterparts. We should note that these tumors also saw an increase in CD86lo CD206hi (M2-like, pro-cancer) macrophages compared to DMSO control (Fig. 2G-iv)., but this increase is expected given that there are generally more macrophages infiltrating these tumors. Despite the increase in M2-like macrophages, these tumors made of MPS1i-treated cells still have roughly 2-fold more M1-like macrophages (Fig. 2G-iv). These results suggest that although other immune cell types infiltrate tumors, which is consistent with upregulated surface expression of H2-Kb (Fig. S4), macrophage functional activity is indeed positively affected by CIN-afflicted cancer cells.
MPS1i-induced CIN favors tumor rejection with IgG opsonization and CD47 disruption
While previous studies show that complete CD47 ablation can favor suppression and even complete rejection of IgG-opsonized B16F10 tumors (Andrechak et al., 2022; Dooling et al., 2022; Hayes et al., 2022; Kamber et al., 2021), inter-experimental variation is high, particularly regarding complete tumor rejection and clearance. Furthermore, how CIN could influence these results is unknown. Therefore, we sought to assess if CIN in B16F10, which thus far has shown to skew macrophages toward an anti-cancer phenotype and away from pro-cancer one, can translate to improved efficacy and consistency in macrophage-oriented therapies. For these subsequent in vivo experiments, we first pre-treated B16F10 cells with either MPS1i or DMSO for 24 h, washed the drug out, and then allowed the cells to recover for an additional 48 h (Fig. 3A). After the recovery period elapsed, we established tumors in mice, with either MPS1i-treated or DMSO-treated B16F10 cells. To further test for CD47-mediated effects, we used either B16F10 CD47 KO or B16F10 sgCtrl, expressing wild-type CD47 (WT) levels. Starting four days post-challenge, mice received either anti-Tyrp1 or mIgG2a isotype control for opsonization.
As expected, all tumors comprised of DMSO-treated B16F10 sgCtrl showed exponential growth and no survivors (Fig. 3B), consistent with previous studies (Dooling et al., 2022; Hayes et al., 2022). These results re-confirm the inhibitory effect that CD47 has on macrophage-mediated immunity (Ingram et al., 2017; Sockolosky et al., 2016; Willingham et al., 2012), suppressing macrophage immune response even with anti-Tyrp1 IgG opsonization. Mice with CIN-afflicted B16F10 sgCtrl tumors ultimately showed exponential growth and no survivors as well, but they did show increased median survival. Furthermore, median survival increased even further when mice were treated with anti-Tyrp1, such that all mice were considered partial responders (survival of 20+ days, one week longer than median survival of tumors comprised of DMSO-treated B16F10 sgCtrl without anti-Tyrp1 treatment). All tumors comprised of DMSO-treated B16F10 CD47 KO showed exponential growth and no survivors (Fig. 3C), regardless of anti-Tyrp1 treatment or not. However, CIN-afflicted B16F10 CD47 KO tumors showed more positive outcomes. Even without anti-Tyrp1, all challenged mice with CIN-afflicted CD47 KO tumors were either cured completely (28%) or considered partial responders. When paired with anti-Tyrp1 treatment, 97% of mice challenged with CIN-afflicted CD47 KO tumors survive.
Long-term survival results show that challenging mice with both MPS1i-treated and DMSO-treated B16F10 sgCtrl failed to yield any survivors, regardless of anti-Tyrp1 opsonization or not (Fig. 3D-i). Similarly, we also failed to generate any survivors among mice challenged with DMSO-treated B16F10 CD47 KO cells, regardless of anti-Tyrp1 opsonization or not (Fig. 3D-ii). This again highlights a challenge in optimizing macrophage-mediated therapies: achieving consistency in long-term therapeutic outcomes. Mice challenged with MPS1i-treated B16F10 CD47 KO cells, however, were able to survive, both without anti-Tyrp1 (28% survival) and with anti-Tyrp1 (97% survival) (Fig. 3D-ii). To determine if the degree of CIN affected survival, we also challenged mice with B16F10 CD47 KO cells treated with varying concentrations of reversine. Regardless of MPS1i concentration, >80% of mice survive when also treated with anti-Tyrp1 (Fig. S6). These results show that, in physiologically relevant microenvironments, early-stage CIN can favor survival when paired with IgG opsonization and CD47 disruption. This further suggests that macrophages are key effector cells in achieving survival against CIN-afflicted tumors, since 97% survival was achieved under conditions of maximal phagocytosis.
Although no mice challenged with CIN-afflicted B16F10 sgCtrl survived, MPS1i increase median survival significantly when paired with anti-Tyrp1 opsonization (Fig. 3D-i). This suggests that macrophages still display some anti-cancer activity, despite the inhibitory effects of CD47, and are important effector cells in final therapeutic outcome. To better support the hypothesis that macrophages are indeed key effector cells in rejecting CIN-afflicted tumors, we established tumors comprised of wild-type (WT) B16F10 in mice. Although WT tumors are generally unaffected by anti-CD47 and anti-Tyrp1 (Dooling et al., 2022; Hayes et al., 2022), we hypothesized that we could eliminate CIN-afflicted WT B16F10 tumors by providing adoptive transfer of marrow to increase macrophage numbers to compensate for the CD47-mediated inhibition of endogenous macrophages. Furthermore, we engineered marrow by priming Fcγ receptors with anti-Tyrp1, initially inhibiting CD47-SIRPα interaction via anti-SIRPα antibody blockade or combining both (Fig. 3E-i). As expected, mice challenged with CIN-afflicted WT tumors and treated with regular, unprimed marrow only had ∼14% survival (Fig. 3E-ii & iii). This result, however, was identical to chromosomally stable WT tumors treated with marrow primed with both anti-SIRPα and anti-Tyrp1. These results suggest increased macrophages numbers do provide some minor benefit. As the marrow engineering becomes more rigorous to maximize phagocytosis, survival improves. 37% of mice challenged with CIN-afflicted WT tumors survived when treated with marrow that was initially engineered with anti-SIRPα. 50% survived with marrow primed with anti-Tyrp1. Lastly, 80% survived with anti-Tyrp1-primed and anti-SIRPα-blocked monocytes (Fig. 3E-ii & iii). Altogether, these results support three conclusions: (1) the increase in survival attributed to anti-SIRPα supports the idea that CD47 modulates therapeutic outcome even during CIN; (2) the increase in survival attributed to anti-Tyrp1 highlights the importance of IgG opsonization; (3) the high success from the combination emphasizes macrophages playing a key role in achieving complete rejection and clearance.
Clearance of CIN-afflicted tumors promotes de novo pro-phagocytic & anti-cancer IgG
Macrophages and related phagocytic cells make up innate immunity, a first line of defense against pathogens and disease, but they are also responsible for initiating acquired immunity. The activation of the acquired immune response involves two branches of immunity: humoral (mediated by macromolecules such as antibodies) and cell-mediated immunity (involving T cells, for example). We hypothesized that mice that survived challenges with CIN-afflicted tumors would show signs of an acquired immune response due to their high survival rate.
We collected convalescent serum from survivors to quantify de novo anti-cancer IgG antibodies that may have resulted from successful rejection and clearance of CIN-afflicted tumors (Fig. 4A). Sera was collected and then subsequently used in antibody binding testing and Western blotting to confirm emergence of anti-B16F10 antibodies. We first quantified IgG2a and IgG2b titers in convalescent sera, both of which have been previously found to engage mouse macrophage Fcγ receptors (Bruhns, 2012; Nimmerjahn et al., 2010) that are typically required for macrophage-mediated phagocytosis. B16F10 cells, either Tyrp1-expresing or Tyrp1 KO, were incubated with sera (convalescent from survivors or naïve from unchallenged mice) and then counterstained with conjugated antibodies against IgG2a/c and IgG2b (Fig. 4B-i & ii). All mice that survived challenges from CIN-afflicted tumors yielded sera that showed significantly large increases in IgG2a/c binding against both Tyrp1-positive and Tyrp1 KO B16F10 cells. We similarly saw a statistically significant increases in IgG2b binding against both Tyrp1-positive and Tyrp1 KO B16F10 cells using the same sera, although the increases in binding were more variable. Altogether, we see that surviving CIN-afflicted tumors generates IgG-rich sera, like previous studies (Dooling et al., 2022; Hayes et al., 2022). The increases in binding observed against Tyrp1 KO cells also suggest that IgG antibodies target a repertoire of antigens beyond Tyrp1, which we further qualitatively confirmed via Western blotting (Fig. 4B-iii). Convalescent sera were used to immunoblot against B16F10 lysates, revealing many bands at multiple molecular weights and more bands than when immunoblotting with naïve sera, supporting our hypothesis of antigen broadening beyond Tyrp1. Lastly, we also tested IgG2a and IgG2b titers from additional in vivo experiments: survivors from both titrated CIN-afflicted tumors from Fig. S6 and adoptive marrow transfers in Fig. 3E. Similarly, convalescent sera from all these mice show increases in IgG2a/c and IgG2b in both Tyrp1-expressing and Tyrp1 KO B16F10 cells (Fig. S7). These results confirm that regardless of the method used to exploit CIN, induction of anti-cancer IgG can be expected.
To test where these de novo serum antibodies functionally promote macrophage-mediated phagocytosis, we performed conventional 2D phagocytosis assays in which cancer cell suspensions were opsonized with sera (or anti-Tyrp1 or mouse IgG2a isotype as controls) (Fig. 4C-i). Under conditions of CD47 KO, we see that nearly all unpurified convalescent sera increased phagocytosis relative to naïve serum and mIgG2a isotype control. Furthermore, this increase in phagocytosis is identical to that provided by anti-Tyrp1 (both ∼5-fold higher than baseline). We also find that sera continue to promote phagocytosis even in B16F10 CD47/Tyrp1 double KO cells (∼3-fold higher than baseline). Convalescent sera are still able to provide increases in phagocytosis against double KO cells, whereas anti-Tyrp1 expectedly does not drive phagocytosis due to lack of antigen. This again supports the hypothesis of acquired immunity with de novo IgG antibodies that target B16F10 antigens beyond Tyrp1. Upon confirming the functional effect of de novo IgG in the convalescent sera, we then wondered if convalescent serum IgG would be able to suppress tumoroid growth, given that this model better captures both the biophysical microenvironment and proliferative capacity of tumors (Dooling et al., 2022). Indeed, we found that convalescent serum IgG added simultaneously with macrophages to B16F10 CD47 KO tumoroids led to either tumoroid elimination or significantly suppressed growth (Fig. 4C-ii), although the efficacy was less potent as anti-Tyrp1. This suggests that perhaps the polyclonal de novo IgGs here still lack the specificity benefits that accompany a monoclonal antibody such as anti-Tyrp1. Nonetheless, these results demonstrate induction of a generally potent anti-cancer antibody response B16F10 in a CD47 KO context.
We then proceeded to test the function of convalescent serum in vivo by opsonizing B16F10 CD47 KO cells just prior to subcutaneous implantation in naïve mice. For comparison, we also opsonized B16F10 CD47 KO cells with either anti-Tyrp1 (positive control) or mIgG2a control (negative control). We found that both convalescent sera and anti-Tyrp1 suppressed tumor growth by days 11 and 13 relative to mIgG2a control (Fig. 4D-i). Interestingly, we found that convalescent sera showed a trend of suppressing growth more than anti-Tyrp1, although this was not statistically significant. We continued to monitor all mice for long-term survival, and we found that this pre-opsonization with both convalescent sera and anti-Tyrp1 eliminated tumors in challenged mice with near identical cure rates (25% and 22%, respectively) (Fig. 4D-ii). Altogether, we found that the convalescent sera from mice originally challenged with chromosomally unstable tumors has potent anti-cancer effects in vitro and in vivo.
Acquired immunity suppresses growth of chromosomally stable tumors and becomes more effective with ongoing challenges of CIN-afflicted tumors
The anti-cancer IgG antibody development in survivors led us to further hypothesize that we should see improved median survival and/or survival rate if surviving mice were re-challenged. We therefore challenged surviving mice with a second injection of either DMSO-treated or B16F10 CD47 KO cells (Fig. 4E). If additional survivors resulted from this experiment, we also intended to undergo a third challenge, akin to a prime-boost strategy for anti-cancer vaccination. It should be highlighted that starting from this second challenge, no mice received anti-Tyrp1. This was done to maximally challenge acquired immunity and to better simulate the possibility of recurrence post-therapy. Age-matched naïve mice receiving their first challenge responded similarly to the younger cohorts (Fig. 3B). Of the previously cured mice, only a single mouse (of 11 total) survived a challenge with DMSO-treated B16F10 cells (Fig. 4F). However, median survival increased (21 days) compared to their naïve counterparts (14 days), supporting the initial hypothesis of prolonged survival. Survivors that were re-challenged again with MPS1i-treated cells, however, showed 100% survival, even in the absence of anti-Tyrp1 (Fig. 4F). Age-matched naïve mice receiving their first challenge of MPS1i-treated cells responded relatively similarly (a single survivor out of 6 total, 17% survival) to the younger cohort (28% survival). This complete success rate against a second challenge of CIN-afflicted B16F10 CD47 KO further supports an acquired immune response, at least against ongoing chromosomally unstable cells.
Mice that failed to reject re-challenge tumors comprised of DMSO-treated B16F10 CD47 KO in Fig. 4F were euthanized and had their tumors harvested to measure their immune cell infiltrate by flow cytometry (Fig. S8). Although these mice did not survive, we found that these previous survivors showed roughly a 2-fold increase in the number of immune cells in their tumors (Fig. 4G-i), a 2-fold increase in the number of CD3+ CD8+ T cells (Fig. 4G-ii), and a near 3-fold increase in the number of F4/80+ macrophages (Fig. 4G-iii) compared to their naïve counterparts. We further found that of the F4/80+ macrophage infiltrate, previous survivors had roughly four times as many MHCII-high macrophages (M1-like, anti-cancer). These immune infiltrate analyses suggest that although the acquired immune response in these previous survivors was still not potent enough to clear chromosomally stable and regularly proliferating B16F10 CD47 KO tumors, it did enhance cell-mediated immunity.
Lastly, we performed a third tumor challenge on second challenge survivors with untreated B16F10 CD47 KO cells (chromosomally stable and regular proliferating). Mice were left untreated post-challenge (no anti-Tyrp1). ∼56% of these mice completely resisted tumor growth. Of the four mice that developed tumors, two had to be euthanized prematurely due to tumor rupture but were indeed showing signs of growth suppression. The last two mice consisted of two long-term partial responders: one whose tumor did not reach terminal burden until 82 days post-challenge and another who experienced stable tumor regression with almost no regrowth. Overall, the 56% survival rate in the third challenge, in the absence of anti-Tyrp1, and the partial responses observed in mice that grew tumors suggest a durable immunological response that results from facing and clearing ongoing chromosomally unstable tumors, at least in the context of CD47 disruption.
Discussion
Macrophage-oriented immunotherapies against solid tumors have maximal efficacy when three elements are combined: large numbers of macrophages for cooperativity, IgG opsonization that activates Fc receptors and stimulates macrophage-mediated phagocytosis, and disruption of the CD47 macrophage immune checkpoint (Dooling et al., 2022). However, even with these factors properly applied, complete tumor rejection and clearance is not guaranteed and varies greatly across in vivo mouse studies (Andrechak et al., 2022; Dooling et al., 2022; Hayes et al., 2022; Kamer et al., 2021). Here, we show a 97% survival rate of mice challenged with CIN-afflicted B16F10 tumors when maximizing macrophage-mediated activity. This result improves upon previous studies and is more impressive given that B16F10 are typically poorly immunogenic, do not respond to either anti-CD47 or anti-PD-1/PD-L1 monotherapies, and show large variability in cure rates (anywhere from 20-40%) even when macrophages should be maximally phagocytic.
These results suggest that CIN in early stages generates anti-cancer vulnerabilities that favor macrophage-mediated immune response, contingent on conditions of maximal phagocytosis. Immunocompetent mice consistently survive these challenges at high survival rates. These survivors also develop de novo anti-cancer IgG, similar to previous studies (Dooling et al., 2022; Hayes et al., 2022), that are pro-phagocytic, multi-epitope, and efficacious in vivo. The emergence of these IgGs could be synergistic with clinically relevant CD47 blockade treatments for solid tumors and help address concerns regarding resistance due to antigen loss (Jalil et al., 2020). Mice that are re-challenged with chromosomally stable CD47 KO tumors (and without exogenous anti-Tyrp1 opsonization) shows increased median survival and increased immune infiltrate, further supporting the hypothesis of newly generated anti-cancer acquired immunity. More interestingly, though, we see that all mice re-challenged with CIN-afflicted CD47 KO tumors survive, even in the absence of anti-Tyrp1 opsonization. A third challenge of these two-time survivors with chromosomally stable CD47 KO tumors shows >50% mice survive and improved median survival for non-survivors. These results elucidate two advantages that CIN can provide to better therapeutic outcomes. First, early-stage CIN facilitates survival while generating potent de novo IgGs that can drive positive phagocytic feedback to minimize recurrence. Second, ongoing vulnerability-inducing CIN in tumor cells can both strengthen cell-mediated acquired immunity and create an antigen reservoir for the maintenance of long-term humoral immunity.
The effects of CIN and aneuploidy in macrophages nonetheless still require more investigation. While secretome from MPS1i-treated cancer cells has been found to trigger expression of Arg1 and Il6 (Xian et al., 2021), both of which are pro-cancer M2-like macrophage markers (Fernando et al., 2014; Mujal et al., 2022), our findings suggest that polarization is much more complex. Here, whole-tumor bulk RNA-sequencing hints at CIN-afflicted tumors having a macrophage population that is both less anti-inflammatory and M2-like. Single-cell RNA-sequencing of BMDMs treated with secretome from CIN-afflicted cells further suggests that CIN induces a microenvironment that can pushes macrophages to a pro-inflammatory, anti-cancer phenotype while minimizing polarization to a pro-cancer phenotype. Our transcriptomics analyses also align more with deeper investigation that suggest additional markers are required for macrophage polarization distinction (Jablonksi et al., 2015). We further confirm these findings by observing increased surface protein expression of anti-cancer M1-like macrophages in vitro 3D tumoroid co-cultures with CIN-afflicted cells and in vivo immune infiltrate experiments. Furthermore, BMDMs show enhanced clearance of CIN-afflicted cells in 3D tumoroid phagocytosis assays. Additionally, the aforementioned study (Xian et al., 2021) only found this trend in SKOV3 cells, whereas their aneuploid fused B16 cells actually show decreased Arg1 expression, suggesting possible cell-intrinsic complications. Meta-analyses of aneuploid tumor genomics data from TCGA also suggest reduced anti-cancer macrophage activity (Davoli et al., 2017; Xian et al., 2021). Our study, however, distinctly highlights macrophage response at early timepoints of CIN, during the which cancer cells have not yet adapted to CIN- and aneuploidy-induced stresses. TCGA studies, on the other hand, are limited to much later timepoints when cells have overcome to CIN- and aneuploidy-associated stresses and developed their own unique aneuploidies to drive tumor progression. Additionally, more recent studies find that aneuploidy and CIN paired with high tumor mutational burdens show increased median survival in patients (Spurr et al., 2022a; Spurr et al., 2022b), suggesting possible other complexities that favor immune infiltration and that could explain how CIN can favor macrophage-mediated immune response.
Materials and Methods
Cell culture
B16F10 cells (CRL-6475) were obtained from American Type Culture Collection (ATCC) and cultured at 37°C and 5% CO2 in either RPMI-1640 (Gibco 11835-030) or Dulbecco’s Modified Eagle Medium (DMEM, Gibco 10569-010) supplemented with 10% fetal bovine serum (FBS, Sigma F2442), 100 U/ mL penicillin and 100 μg/mL streptomycin (1% P/S, Gibco 15140-122). B16F10 cells were maintained in passage in RPMI-1640 but switched to DMEM at least three days prior to in vivo subcutaneous injections. 293T human embryonic kidney (CRL-1573) cells were also obtained from ATCC and cultured at 37°C and 5% CO2 in DMEM culture media supplemented with 10% FBS and 1% P/S. All cell lines were passaged every 2-3 days when a confluency of ∼80% was reached. For trypsinization, cells were washed once with Dulbecco’s phosphate-buffered saline (PBS, Gibco 14190-136) and then detached with 0.05% Trypsin (Gibco 25300-054) for 5 min at 37°C and 5% CO2. Trypsin was quenched with an equal volume of complete culture media.
Lentiviral production and transduction
24 h prior to lentivirus production, 293T cells were plated at a density of 8×105 cells in 2 mL of DMEM in individual wells of a 6-well plate. On the day of transfection, 1.35 μg of psPAX2, 165 ng of pCMV-VSV-G, and 1.5 μg of lentiviral plasmid were added into a microcentrifuge tube with 7.5 μL of Mirus TransIT-Lenti transfection reagent (Mirus Bio, 6604). Once all plasmids were pooled together with the transfection reagent, 300 μL of serum-free media was added. The solution was gently mixed by pipetting and then allowed to incubate for 30 min. After 30 min elapsed, the solution was gently added dropwise by pipetting to an individual 6-well containing the 293T cells plated the day prior. We note that we also use B16F10 CD47 and Tyrp1 KO lines in this study, whose preparation has been previously described (Hayes, et al., 2020). LentiV-cas9_puro and Lenti_sgRNA_EFS_GFP plasmids (Addgene #108100 and 65656, respectively) were gifts from Christopher Vakoc. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260). pVSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). The single guide RNA (sgRNA) oligonucleotides (CD47, 5′-TCCCCGTAGAGATTACAATG-3′; SIRPα, 5′-TAATTCTAAGGTCATCTGCG-3′) were designed using the Broad CRISPR algorithm. sgRNAs were cloned into the sgRNA vector using a BsmBI restriction digest.
Viral production was allowed to continue for 48 h, after which the supernatant was collected from each well and centrifuged for 5 min at 300 x g. The supernatant was collected and then either added directly to B16F10 cells (0.5 mL of lentivirus-containing supernatant to 2×104 cells in an individual 6-well) or stored at -70°C for long-term storage. 72 h after transduction, spent media with lentivirus was aspirated, B16F10 cells were washed with PBS, and fresh media was added. For selecting successfully lentivirally transduced cells, cells were cultured in fresh media containing 1 μg/mL of puromycin (Invitrogen A1113803). Cells were kept in puromycin-containing until a non-transduced control population also treated with puromycin completely died.
Antibodies
Antibodies used for in vivo treatment and blocking and for in vitro phagocytosis are as follows: anti-mouse/human Tyrp1 clone TA99 (BioXCell BE0151), mouse IgG2a isotype control clone C1.18.4 (BioXCell BE0085), and Ultra-LEAF anti-mouse CD172a (SIRPα) clone P84 (BioLegend 144036). Low-endotoxin and preservative-free antibody preparations were used for in vivo treatments and in vitro phagocytosis experiments. For primary antibody staining of surface proteins via flow cytometry, the following were used: anti-mouse CD47 clone MIAP301 (BioXCell BE0270) and anti-mouse/human Tyrp1 clone TA99. Secondary antibodies used for flow cytometry are as follows: Alexa Fluor 647 donkey anti-mouse IgG (ThermoFisher A-31571) and Alexa Fluor 647 goat anti-rat IgG (ThermoFisher A-21247). All secondary antibody concentrations used followed the manufacturer’s recommendations.
For immune infiltrate analysis, the following BioLegend antibodies were used: Brilliant Violet 650 anti-mouse CD45 clone 30-F11 (103151), Brilliant Violet 785 anti-mouse CD45 clone 30-F11 (103149), APC/Cy7 anti-mouse CD45 clone 30-F11 (103115), APC anti-mouse/human CD11b clone M1/70 (101212), PE/Cyanine7 anti-mouse/human CD11b clone M1/70 (101216), PE/Dazzle 594 anti-mouse Ly6G clone 1A8 (127647), PerCP anti-mouse Ly-6G clone 1A8 (127653), PE anti-mouse F4/80 clone BM8 (123110), Brilliant Violet 605 anti-mouse Ly-6C clone HK1.4 (128035), APC anti-mouse I-A/I-E clone M5/114.15.2 (107614), Pacific Blue anti-mouse CD86 clone GL-1 (105022), APC/Cy7 anti-mouse CD86 clone GL-1 (105029), APC anti-mouse CD206 (MMR) clone C068C2 (141707), Brilliant Violet 421 anti-mouse CD206 clone C068C2 (141717), PE/Cy7 anti-mouse CD163 clone S15049F (156707), APC/Cy7 anti-mouse CD3e clone 145-2C11 (100329), and Alexa Fluor 647 anti-mouse CD8a clone 53-6.7 (100727). TruStain FcX PLUS (anti-mouse CD16/32) clone S17011E (156603) was used in all immune infiltrate experiments to block Fc receptors. For immunogenicity post-MPS1i treatment, APC anti-mouse H-2kb/H-2Db clone 28-8-6 (114613) was used. For IgG titer in tumor challenge surviving mice, the following BioLegend antibodies were used: PE anti-mouse IgG2a clone RMG2a-62 (407108, known to bind IgG2c as well) and APC anti-mouse IgG2b clone RMG2b-1 (406711). Primary antibody used in Western blotting was anti-β-actin clone C4 (Santa Cruz sc 47778). Secondary antibody used in Western blotting was HRP sheep anti-mouse IgG (GE Life Sciences NA931V).
Mice
C57BL/6 mice (Jackson Laboratory 000664) were 6-12 weeks old at the time of tumor challenges and for bone marrow harvesting, with the exception of second and third challenge experiments. Additionally, for re-challenge experiments, age-matched naïve mice were used. All experiments were performed in accordance with protocols approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Pennsylvania.
Drug treatments & micronuclei quantification
For MPS1i studies, the following chemical drugs were used: reversine (Cayman Chemical 10004412), AZ3146 (Cayman Chemical 19991), and BAY 12-17389 (Selleck Chemicals S8215). 24 h prior to treatment, B16F10 cells were in either 6-well or 12-well plates. For 6-wells, 20,000 cells were plated per well. For 12-wells, 2,000 cells were plated per well. On the day of treatment, spent media was aspirated, and fresh media supplemented with 10% FBS, 1% P/S, and either MPS1i or DMSO vehicle control was added to each well. The concentration used for each treatment is listed in the Figure legend. The volume of DMSO added was equal to the volume required for the highest MPS1i concentration for each experiment. All cells were treated for 24 h, after which drug-containing spent media was aspirated. Cells were then washed with a full volume of PBS for 5 min. PBS was aspirated, and two repeat washes were performed. Cells were then allowed to recover from MPS1i treatment for an additional 48 h, with a fresh media replacement 24 h after the initial wash.
For imaging and micronuclei quantification, cells were fixed with 4% formaldehyde for 20 min after the 48 h recovery period and later imaged on an Olympus IX inverted microscope with a 40x/0.6 NA objective. The Olympus IX microscope was equipped with a Prime sCMOS camera (Photometrics) and a pE-300 LED illuminator (CoolLED) and was controlled with MicroManager software v2. At least 200 B16F10 were imaged per individual well for micronuclei quantification.
Bone marrow-derived macrophages (BMDMs)
Bone marrow was harvested from the femurs and tibia of donor mice, lysed with ACK buffer (Gibco A1049201) to deplete red blood cells, and then cultured on Petri culture dishes for 7 days in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco 12440-053) supplemented with 10% FBS, 1% P/S, and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating, one whole volume of fresh IMDM supplemented 10% FBS, 1% P/S, and 20 ng/mL M-CSF. After 7 days of differentiation, spent media was removed, BMDMs were gently washed once with phosphate-buffered saline (PBS), and fresh IMDM supplemented with 10% FBS, 1% P/S, and 20 ng/mL M-CSF was added.
Conditioned media treatment of BMDMs
BMDMs that had successfully undergone 7 days of differentiation in 20 ng/mL M-CSF were used. Spent media was removed, BMDMs were gently washed once with phosphate-buffered saline (PBS), and fresh IMDM supplemented 10% FBS, 1% P/S, and 20 ng/mL M-CSF was added. Then, conditioned media from B16F10 cells treated with either MPS1i or DMSO was collected. Conditioned media was centrifuged for 5 min at 300 x g to remove any cellular debris. The supernatant was collected and then supplemented to 5% FBS, 1% P/S, and 20 ng/mL M-CSF. One whole volume of conditioned media was added to BMDMs with fresh media. 24 h after treatment, BMDMs were detached using 0.05% Trypsin and processed for single-cell RNA-sequencing.
In vitro phagocytosis
For two-dimensional phagocytosis assays, BMDMs were detached using 0.05% Trypsin and re-plated in either 6-well or 12-well plates, at a density of 1.8×104 cells per cm2 in IMDM supplemented 10% FBS, 1% P/S, and 20 ng/mL M-CSF. After 24 h elapsed, BMDMs were labeled with 0.5 μM CellTracker DeepRed dye (Invitrogen C34565), according to the manufacturer’s protocol. Following staining, BMDMs were washed and incubated in serum-free IMDM supplemented 0.1% (w/v) BSA and 1% P/S. B16F10 cells were labeled with carboxyfluorescein diacetate succinimidyl ester (Vybrant CFDA-SE Cell Tracer, Invitrogen V12883), also according to the manufacturer’s protocol. B16F10 cells were detached and opsonized with 10 μg/mL anti-Tyrp1, with 10 μg/mL mouse IgG2a isotype control antibody, or 5% (v/v) mouse serum in 1% BSA. Opsonization was allowed for 30-45 min on ice. Opsonized B16F10 suspensions were then added to BMDMs at a ∼2:1 ratio and incubated at 37°C and 5% CO2 for 2 h. Non-adherent cells were removed by gently washing with PBS. For imaging, cells were fixed with 4% formaldehyde for 20 min and later imaged on an Olympus IX inverted microscope with a 40x/0.6 NA objective. The Olympus IX microscope was equipped with a Prime sCMOS camera (Photometrics) and a pE-300 LED illuminator (CoolLED) and was controlled with MicroManager software v2. At least 300 macrophages were imaged per individual well for calculation of phagocytosis.
3D tumoroid formation and phagocytosis
Briefly, non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h. The cells were then washed with 100 μL of complete RPMI 1640 cell culture media. This generated surfaces conducive to generating tumoroids and preventing cells from adhering to the well bottom during experiments. B16F10 were detached by brief trypsinization, resuspended at a concentration of 1×104 cells per mL in complete RPMI 1640 cell culture media (10% FBS, 1% P/S) with 50 μM β-mercaptoethanol (Gibco 21985023). 100 μL of this cell suspension was added to each well such that each tumoroid initially started with approximately 1×103 cells. Aggregation of B16F10 cells was confirmed 24 h later by inspection under microcopy. Upon confirmation of tumoroid formation, BMDMs were labeled with 0.5 μM CellTracker DeepRed dye (Invitrogen C34565), according to the manufacturer’s protocol. BMDMs were then detached by brief trypsinization and gentle scraping and resuspended in complete RPMI 1640 cell culture media at a concentration that would allow for delivery of 3×103 BMDMs to each individual tumoroid culture. The BMDM cell suspension was also supplemented with 120 ng/mL M-CSF and antibodies (either anti-Tyrp1 or mouse IgG2a isotype control) such that delivery of 20 μL of this suspension to each individual tumoroid culture result in final concentrations of 20 ng/mL M-CSF and 20 μg/mL of antibody. For tumoroid studies in which mouse convalescent serum was used, the BMDM cell suspension was supplemented with 120 ng/mL M-CSF and serum such that delivery of 20 μL of this suspension to each individual tumoroid culture resulted in final concentrations of 20 ng/mL M-CSF and a final mouse serum concentration of 1:200. Tumoroids were imaged on an Olympus IX inverted microscope with a 4x/0.6 NA objective.
For macrophage polarization tumoroid experiments (Fig. 2B-C), B16F10 tumoroids were prepared in the same manner as described above, with the exception that no anti-Tyrp1 or mIgG2a was added. After 5 days had elapsed for the experiment, tumoroids were dissociated by brief trypsinization and stained with conjugated antibodies targeting MHCII, CD86, CD163, and CD206, following the manufacturer’s protocol. Cells were then run on a BD LSRII (Benton Dickinson) flow cytometer. Data were analyzed with FCS Express 7 software (De Novo Software).
In vivo tumor models
B16F10 cells cultured in DMEM growth media were detached by brief trypsinization, washed twice with PBS, and resuspended at 2×106 cells per mL. Cell suspensions were kept on ice until injection. All subcutaneous injections were performed on the right flank while mice were anesthetized under isoflurane. Fur on the injection site was wet slightly with a drop of 70% ethanol and brushed aside to better visualize the skin. A 100 μL bolus containing 2×105 cells cancer cells was injected beneath the skin. For treatments, mice received either intravenous or intraperitoneal injections of anti-Tyrp1 clone TA99 or mouse IgG2a isotype control clone C1.18.4 (250 μg antibody in 100 μL PBS) on days 4, 5, 7, 9, 11, 13, and 15 post-tumor challenge. Intravenous injections were done via the lateral tail vein. Tumors were monitored by palpation and measured with digital calipers. The projected area was roughly elliptical and was calculated as A = π/4 x L x W, where L is the length along the longest axis and W is the width measured along the perpendicular axis. For our studies, a projected area of 125 mm2 was considered terminal burden for survival analyses. Mice were humanely euthanized following IACUC protocols if tumor size reached 2.0 cm on either axis, if tumor reached a projected area greater than 200 mm2 or if a tumor was ulcerated.
Adoptive cell transfers
Fresh bone marrow was harvested as described in the Bone marrow-derived macrophages (BMDMs) methods section. Marrow cells were counted on a hemocytometer and resuspended to a concentration of 8×107 cells per mL in in 5% (v/v) FBS/PBS. To block SIRPα, cells were then incubated with anti-SIRPα clone P84 (18 μg/mL) for 1 h at room temperature on a rotator. After the incubation period elapsed, cells were centrifuged at 300 x g for 5 min, washed with PBS, and then centrifuged once more at 300 x g for 5 min to remove any unbound anti-SIRPα. Cells were again re-suspended to a concentration of 8×107 cells per mL in in 2% (v/v) FBS/PBS, with or without anti-Tyrp1 clone TA99 (1 mg/mL). Marrow cells were then injected intravenously (2×107 cells in 250 μL per mouse) into tumor-bearing mice. All adoptive transfers were done four days post-challenge. Control data were adapted from (Dooling et al., 2022) to minimize mice for experiments, since the cited study establishes proper benchmarks for comparison and also finds that these control conditions minimally (if at all) improve survival.
Serum collection & IgG titer quantification
Blood was drawn retro-orbitally from mice anesthetized under isoflurane, using heparin- or EDTA-coated microcapillary tubes. Collected blood was allowed to clot for 1 h at room temperature in a microcentrifuge tube. The serum was separated from the clot by centrifugation at 1,500 x g and stored at -20°C for use in flow cytometry and phagocytosis assays.
For IgG titer quantification, B16F10 cells were detached by trypsinization and incubated with 5% (v/v) mouse serum in 1% BSA. Opsonization was allowed for 30-45 min on ice. After the incubation period elapsed, cells were centrifuged at 300 x g for 5 min, washed once with PBS, centrifuged again at 300 x g for 5 min, and then resuspended in 0.1% (w/v) BSA with both PE anti-mouse IgG2a/c clone RMG2a-62 and APC anti-mouse IgG2b clone RMG2b-1 (see Antibodies section for more information). Anti-IgG conjugated-antibody incubation occurred for 30-45 min, after which cells were centrifuged at 300 x g for 5 min, washed once with PBS, centrifuged again at 300 x g for 5 min, and then resuspended in 5% (v/v) FBS/PBS. Cells were run on a BD LSRII (Benton Dickinson) flow cytometer.
Western blotting
Lysate was prepared from B16F10 cells using RIPA buffer containing 1X protease inhibitor cocktail (Millipore Sigma P8340) and boiled in 1X NuPage LDS sample buffer (Invitrogen NP0007) with 2.5% β-mercaptoethanol. Proteins were separated by electrophoresis in NuPage 4-12% Bis-tris gels run with 1X MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002). The membranes were blocked with 5% (w/v) non-fat milk in Tris buffered saline (TBS) plus Tween-20 (TBST) for 1 h and stained with 5% (v/v) mouse serum overnight at 4°C with agitation. The membranes were washed TBST and incubated with 1:500 secondary antibody conjugated with horseradish peroxidase (GRP) in 5% (w/v) milk in TBST for 1 h at room temperature with agitation. The membranes were then washed again three times with TBST. Membranes probed with HRP-conjugated secondary antibody were developed a 3,3’,5,5’-teramethylbenzidine (TMB) substrate (Genscript L002V or Millipore Sigma T0565). Developed membranes were scanned and then processed with ImageJ.
Immune infiltrate analysis of tumors
For day 5 post-challenge measurements: If mice required anti-Tyrp1 treatment, mice received a single dose of intravenously delivered anti-Tyrp1 or mouse IgG2a isotype control four days (96 h) post-tumor challenge. 24 later, mice were humanely sacrificed. Otherwise, mice were sacrificed five days post-tumor challenge. For immune analysis of second challenge non-survivors: Mice were humanely euthanized when tumor burden reached >150 mm2.
Tumors from euthanized mice were excised and placed into 5% (v/v) FBS/PBS. Tumors were then disaggregated using Dispase (Corning 354235) supplemented with 4 mg/mL of collagenase type IV (Gibco 17104-019) and DNAse I (Millipore Sigma, 101041159001) for 30-45 min (until noticeable disaggregation) at 37°C, centrifuged for 5 min at 300 x g, and resuspended in 1 mL of ACK lysis buffer for 12 min at room temperature. Samples were centrifuged for 5 min at 300 x g, washed once with PBS, and then resuspended in 5% (w/v) BSA/PBS for 20 min. After 20 min elapsed, fluorophore-conjugated antibodies to immune markers were added to each cell suspension. The following markers were used for analysis: for macrophages, CD45, CD11b, F4/80, Ly-6C, Ly-6G, CD86, CD206, MHCII; for T cells, CD45, CD3e, CD8a. Antibody binding occurred for 30 mins while samples were kept on ice and covered from light. Samples were then centrifuged for 5 min at 300 x g, washed once with PBS, and resuspended in FluoroFix Buffer (BioLegend, 422101) for 1 h at room temperature prior to analysis on a BD LSRII (Benton Dickinson) flow cytometer. Data were analyzed with FCS Express 7 software (De Novo Software).
Bulk RNA-sequencing
RNA library was constructed using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina (E7770S, E7335) per the manufacturer’s instructions. The library prepared was processed at the Next Generation Sequencing Core at the University of Pennsylvania (12-160, Translational Research Center) using NovaSeq 6000, 100 cycles (Illumina). The reads were aligned to mouse reference, mm10 (GENCODE vM23/Ensembl 98) using star alignment. Cell count matrix was generated and imported to RStudio for downstream analysis. Package “DESeq2” (v1.32.0) was used for normalization and differential expression analysis. Package “fgsea” (v1.18.0) was used for gene set enrichment analysis. Additional exploratory data analysis was then done using either RStudio or Python 3.8.
Single-cell RNA-sequencing
RNA libraries were prepared using the 10X Genomics Chromium Single Cell Gene Expression kit (v3.1, single index, PN-1000128; PN-1000127; PN-1000213) per the manufacturer’s instructions. The libraries were sequenced at the Next Generation Sequencing Core using NovaSeq 6000, 100 cycles (Illumina). Raw base call (BCL) files were analyzed using CellRanger (version 5.0.1) to generate FASTQ files, and the “count” command was used to generate raw count matrices aligned to mm10 (GENCODE vM23/Ensembl 98). Cells were filtered to make sure that they expressed a minimum of 1,400 genes with less than 15 percent mitochondrial content. Data was normalized using the “LogNormalize’’ method from the Seurat package. Differential expression analysis was performed using the “FindAllMarkers’’ command, and the output was used for gene set enrichment analysis (GSEA).
InferCNV
Count matrix of single cell RNA-sequencing results was used as input for InferCNV object construction (1.7.1) (InferCNV of the Trinity CTAT Project, see the following for more information: https://github.com/broadinstitute/inferCNV). Gene position files were created for GRCm38. Single-cell RNA-sequencing data of DMSO-treated B16F10 were used as reference for copy number profile construction. Cell types were annotated either manually or using the package “SingleR” (v1.6.1). For manual annotation, cells were clustered and assigned cell types based on the expression of cell type specific signature genes (SI.5a). Denoised results from InferCNV were used as the input (“infercnv.observations.txt”). The averaged copy number of each chromosome segment was calculated, and the difference between each cell’s copy number and the overall mean at each segment was calculated. The deviation was summed across the entire chromosome to obtain the distribution of the deviation. Cells sharing an absolute deviation that is more than 2.5 times standard deviation away from the distribution peak were marked as outliers for a certain chromosome in question.
Transcriptomic gene sets
For analyzing both bulk and single-cell RNA-sequencing data sets, either hallmark gene sets (from the Human Molecular Signatures Database) or customized gene sets were used. Customized gene sets were used exclusively for macrophage-associated analyses and were made by combining gene sets from the following: Ahn et al., 2018; Cerezo-Wallis et al., 2020; Cunha et al., 2018; Mujal et al., 2022; Perry et al., 2019; Zhou et al., 2020.
Statistical analysis & curve fitting
Statistical analyses and curve fitting were performed in GraphPad Prism 9.4. Details for each analysis are provided in the figure legends. Tumor and tumoroid growth curve data (projected area vs time) were fit to the exponential growth model (A = A0ekt for tumors and A = A1ek(t-1) for tumoroids) using non-linear least squares regression with prefactors A0 or A1 and k, the exponential growth rate. For differential gene expression analysis for both bulk RNA-sequencing and single-cell RNA-sequencing datasets, statistical analyses were done using either RStudio 2022.02.3+492 or Python 3.8.
Code availability
Sequencing data were analyzed using RStudio 2022.02.3+492 and the Seurat package. Additional analyses after normalization were done on either RStudio or Python 3.8. No new code central to the conclusions of this study was developed.
Acknowledgements
This work was supported by funding from the following sources: NIH R01 HL124106 (DED), U54 CA193417 (DED), NSF GRFP DGE-1845298 (BHH, JCA, MPT), and NIH F32 CA228285 (LJD).
The authors acknowledge the following University of Pennsylvania core facilities: Cell Center Stockroom, the Penn Cytomics and Cell Sorting Resource Laboratory, the Penn Genomic and Sequencing Core, and the Cell & Development Biology Microscopy Core.
Competing interests
The authors declare no competing interests.
Data availability statement
All data are available within the article and its supplementary information. Data can be provided upon reasonable request from the corresponding author.
Supplementary Figure Legends
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