Caspase-mediated NPC trimming occurs during neurogenesis and ER stress.
(A) Schematic representation caspase-mediated NPC trimming. (B) Down-/up-regulation of survivin, caspase-3, and myogenin in differentiating C2C12 ls. (C) Relative timing of key events in myogenesis. (D) Expression levels of Nup153, PARP, active caspase-3, survivin, d neuronal differentiation markers (Sox2 and βIII-tubulin) were determined in differentiating neurons. (E) Acute ER stress uced using 1 μg/mL tunicamycin in C2C12 myotubes and reserve cells. The levels of Nups, BiP, XIAP, myosin heavy chain HC), myogenin, and active caspase-3 levels were monitored by immunoblotting. (F) Chronic ER stress was induced in erentiated C2C12 cells for 7 days using low doses of tunicamycin. Nups, BiP, and active caspase-3 levels were assessed.