Mechanism of substrate binding and transport in BASS transporters

Reviewer #1 (Public Review):

The current manuscript provides a timely contribution to the ongoing discussion about the mechanism of the apical sodium/bile acid transporter (ASBT) transporters. Recent structures of the mammalian ASBT transporters exhibited a substrate binding mode with few interactions with the core domain (classically associated with substrate binding), prompting an unusual proposal for the transport mechanism. Early structures of ASBT homologues from bacteria also exhibit unusual substrate binding in which the core substrate binding domain is less engaged than expected. Due to the ongoing questions of how substrate binding and mechanism are linked in these transporters, the authors set out to deepen our understanding of a model ABST homolog from bacteria N. meningitidis (ABST-NM).

The premise of the current paper is that the bacterial ASBT homologs are probably not physiological bile acid transporters, and that structural elucidation of a natively transported substrate might provide better mechanistic information. In the current manuscript, the authors revisit the first BASS homologue to be structurally characterized, ABST-NM. Based on bacteriological assays in the literature, the authors identify the coenzyme A precursor pantoate as a more likely substrate for ABST-NM than taurocholate, the substrate in the original structure. A structure of ASBT-NM with pantoate exhibits interesting differences in structure. The structures are complemented with MD simulations, and the authors propose that the structures are consistent with a classical elevator transport mechanism.

The structural experiments are generally solid, although showing omit maps would bolster the identification of the substrate binding site. One shortcoming is that, although pantoate binding is observed, the authors do not show transport of this substrate, undercutting the argument that the pantoate structure represents binding of a "better" or more native substrate. Mechanistic proposals, like the proposed role of T112 in unlocking the transporter, would be much better supported by transport data.

Reviewer #2 (Public Review):

The manuscript starts with a demonstration of pantoate binding to ASBTnm using a thermostability assay and ITC, and follows with structure determinations of ASBTnm with or without pantoate. The structure of ASBTnm in the presence of pantoate pinpoints the binding site of pantoate to the "crossover" region formed by partially unwinded helices TMs 4 and 9. Binding of pantoate induces modest movements of side chain and backbone atoms at the crossover region that are consistent with providing coordination of the substrate. The structures also show movement of TM1 that opens the substrate binding site to the cytosol and mobility of loops between the TMs. MD simulations of the ASBT structure embedded in lipid bilayer suggests a stabilizing effect of the two sodium ions that are known to co-transport with the substrate. Binding study on pantoate analogs further demonstrates the specificity of pantoate as a substrate.

The weakness of the manuscript includes a lack of transport assay for pantoate and a lack of demonstration that the observed conformational changes in TM1 and the loops are relevant to the binding or transport of pantoate.

Overall, the structural, functional and computational studies are solid and rigorous, and the conclusions are well justified. In addition, the authors discussed the significance of the current study in a broader perspective relevant to recent structures of mammalian BASS members.

Reviewer #3 (Public Review):

The manuscript describes new ligand-bound structures within the larger bile acid sodium symporter family (BASS). This is the primary advance in the manuscript, together with molecular simulations describing how sodium and the bile acids sit in the structure when thermalized. What I think is fairly clear is that the ligands are more stable when the sodiums are present, with a marked reduction in RMSD over the course of repeated trajectories. This would be consistent with a transport model where sodium ions bind first, and then the bile acid binds, followed by a conformational change to another state where the ligands unbind.

While the authors mention that BASS transporters are thought to undergo an elevator transport mechanisms, this is not tested here. In my reading, all the crystal structures describe the same conformational state, and the simulations do not make an attempt to induce a transition on accessible simulation timescales. Instead, there is a morph between two states where different substrates are bound, which induces a conformational change that looks unrelated to the transport cycle.

Instead, the focus is on what kinds of substrates bind to this transporter, interrogating this with isothermal calorimetry together with mutations. With a Kd in the micromolar range, even the best binder, pantoate, actually isn't a particularly tight binder in the pharmaceutical sense. For a transporter, tight binding is not actually desirable, since the substrate needs to be able to leave after conformational change places it in a position accessible to the other side.

There is one really important point that readers and authors should be aware of. In Figure 2A, the names are not consistent with the chemical structure. "-ate" denotes when a carboxylic acid is in the deprotonated form, creating a charged carboxylate. What is drawn is pantoic acid, ketopantoic acid, and pantoethenic acid. Less importantly, the wedges and hashes for the methyl group are arguably not appropriate, since the carbon they are attached to is not a chiral center. For the crystallization, this makes no difference, since under near-neutral pKas the carboxylic acid will spontaneously deprotonate, and the carboxylate form will be the most common. However, if the structures in Figure 2A were used for classical molecular simulation, that would be a big problem, since now that would be modeling the much rarer neutral form rather than the charged state. I am reasonably sure based on Figure 5 that the MD correctly modeled the deprotonated form with a carboxylate, but that is inconsistent with Figure 2A. Otherwise, the structure and simulation analysis falls into the mainstream of modern structural biology work.