Peer review process
Not revised: This Reviewed Preprint includes the authors’ original preprint (without revision), an eLife assessment, public reviews, and a response from the authors (if available).
Read more about eLife’s peer review process.Editors
- Reviewing EditorElaine FuchsHoward Hughes Medical Institute, The Rockefeller University, New York, United States of America
- Senior EditorDidier StainierMax Planck Institute for Heart and Lung Research, Bad Nauheim, Germany
Joint Public Review:
This paper's strengths are the interesting analysis of TLR signaling in hair follicle stem cell activation and the striking phenotype of the TLR2 cKO mice (but note below). The functional interrogation parts using HFSC-specific TLR2 genetic deletion are solid, and an endogenous regulator, CEP, is identified. The experiments reported in this manuscript are well-designed and presented. The authors provided extensive evidence supporting the roles of TLR2 signaling in regulating hair follicle stem cell functions. Importantly, the findings from this paper may have sustained impacts on our understanding of the roles of innate immunity in regulating tissue regeneration in the absence of inflammation.
The main evidence for the mechanistic analysis is based on fluorescence using immunohistochemistry, and here the expression analysis is not convincing. In addition, additional assays beyond immunolandscaping are needed to confirm the findings. The reviewers felt that your data substantiating the mechanism of interaction between TLR2 and BMP pathway needs bolstering.
