Binding of lncDACH1 to dystrophin and the effects of cardiomyocyte-specific transgenic overexpression of lncDACH1 (lncDACH1-TG) on the expression and function of sodium channel.
a, Blotting of dystrophin pulled-down by lncDACH1. b, LncDACH1 precipitated by the antibody of dystrophin. N=4. * P<0.05 vs IgG by one-way ANOVA, followed by Tukey’s post-hoc analysis. c,d, The total, membrane and cytoplasm levels of dystrophin and Nav1.5 by Western blot. N-cadherin is the loading control for membrane extracts. N=10-11 for total protein; N= 7-14 for membrane protein; N= 8-14 for cytoplasm protein. *P<0.05 vs WT group. P-values were determined by unpaired t test. e, Distribution of lncDACH1, dystrophin and Nav1.5 in isolated cardiomyocytes. f, The mRNA levels of dystrophin and SCN5A. N=8-11. g, Peak INa currents, I-V curve and kinetics of INa. N=9-15 cells from 3 mice. *P<0.05 vs WT group. h, Conduction velocity of perfused hearts by optical mapping recordings. N=7. *P<0.05 vs WT group. P-values were determined by unpaired t test.