Disease phenotypes.
(A) Illustration of the scale used to evaluate the severity of symptoms: (0) no symptoms or healthy plant (1) mild symptoms without chlorosis (2) visible chlorosis, (3) advanced chlorosis, (4) strong chlorosis and incipient necrosis, (5) necrosis and death of the plant. (B) Correlation matrix between disease phenotypes in response to the ancestral (upper left) and evolved (lower right) isolates. The diagonal shows correlations between the same phenotype in response to each viral isolate. (C) SNP heritability for each trait in response to each virus. (D-G) Disease phenotypes across lines in response to the ancestral (red) and evolved (blue) TuMV isolates. (H-J) Differences in disease phenotypes in response to each viral isolate (evolved minus ancestral). (K) Number of lines showing necrosis in response to the ancestral or/and evolved isolates; 1 indicates the presence of necrosis. For clarity, the 997 lines showing no necrosis for either isolate (0/0) are not shown.