Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underlying molecular mechanisms remain poorly understood. Here, we investigated the impact of elevated temperature on centromere structure and chromosome segregation during meiosis in Arabidopsis thaliana. Our findings reveal that heat stress causes a significant decline in fertility and leads to the formation of micronuclei in pollen mother cells, along with an extended duration of meiotic division. We also demonstrate a reduction in the amounts of centromeric histone and the kinetochore protein BMF1 at meiotic centromeres with increasing temperature. Furthermore, we show that heat stress prolongs the activity of the spindle assembly checkpoint during meiosis I, indicating impaired efficiency of the kinetochore attachments to spindle microtubules. Our analysis of mutants with reduced amounts of centromeric histone suggests that weakened centromeres sensitize plants to elevated temperature, resulting in meiotic defects and reduced fertility even at moderate temperatures. These results indicate that the structure and functionality of meiotic centromeres in Arabidopsis are highly sensitive to heat stress, and suggest that centromeres and kinetochores may represent a crucial bottleneck in plant adaptation to increasing temperatures.
This important study reports how heat stress affects centromere integrity by compromising the loading of the centromere protein CENH3 and by prolonging the spindle assembly checkpoint during male meiosis in Arabidopsis thaliana. The evidence supporting the claims by live cell imaging is convincing, although deeper mechanistic insight is lacking, making the study overall somewhat preliminary in nature. This work will be of interest to a broad audience of biologists working on how chromatin states are affected by stress conditions.
Global warming impacts crop productivity with more frequent and extreme heatwaves being particularly damaging (Bras et al., 2021). The lower yields associated with heatwaves are mainly attributed to the impairment of the plant reproductive system (Lippmann et al., 2019). Thus, a fundamental understanding of how heat stress affects plant reproduction can guide breeding strategies towards generating climate change resilient crops. Heat stress impairs both male and female reproductive structures, but male gametogenesis is particularly sensitive to higher temperatures (Zinn et al., 2010). Studies in crop and non-crop species have shown that heat stress affects pollen count, morphology, viability, dehiscence, germination and pollen tube growth (De Storme and Geelen, 2014; Chaturvedi et al., 2021).
Acute heat stress applied at different stages of flower development has been found to have the most detrimental effect during microsporogenesis (Pecrix et al., 2011; Hedhly et al., 2020). Meiosis, a reductive cell division that halves number of chromosomes in two consecutive rounds of chromosome segregation, is a crucial step of microsporogenesis. It is a relatively slow process, lasting approximately 1.5 to 2 days in Arabidopsis and barley (Armstrong et al., 2003; Higgins et al., 2012; Prusicki et al., 2019; Valuchova et al., 2020). Prophase I, the longest phase of meiosis, is characterized by pairing and recombination of homologous chromosomes to form stable bivalents. It is followed by two chromosome segregation cycles that first divide homologous chromosomes and then the sister chromatids. Heat stress affects various aspects of plant meiosis. In prophase I, elevated temperature alters the rate and distribution of meiotic recombination (Higgins et al., 2012; Lloyd et al., 2018; Modliszewski et al., 2018) and a recent study in Arabidopsis has shown that heat shock response pathway directly regulates the recombination machinery (Kim et al., 2022). More severe heat stress can impair synapsis and pairing of homologous chromosomes (Loidl, 1989; De Storme and Geelen, 2020; Ning et al., 2021). This is likely due to inefficient completion of homologous recombination, which is monitored by specialized pachytene checkpoint (De Jaeger-Braet et al., 2022).
Heat stress affects the later stages of meiosis as well. Elevated temperatures disturb spindle orientation during meiotic divisions and the formation of radial microtubule arrays, resulting in aberrant cytokinesis and diploid microspores (Pecrix et al., 2011; De Storme and Geelen, 2020). Furthermore, acute heat stress leads to chromosome mis-segregation and the formation of micronuclei (Wang et al., 2017; De Storme and Geelen, 2020; De Jaeger-Braet et al., 2022). The meiotic micronuclei and chromosomal aberrations may arise as a consequence of abnormal repair of meiotic DNA breaks or homologous recombination intermediates in prophase I. However, they can also be caused by defects in chromosome segregation during meiotic divisions (Fenech et al., 2011). Proper attachment of kinetochores to the spindle microtubules before anaphase is crucial for faithful chromosome partitioning to daughter cells, and this process is controlled by spindle assembly checkpoint. Therefore, micronuclei typically occur in mutants with impaired centromere/kinetochore structure or spindle assembly checkpoint (SAC) when treated with spindle inhibitors (Kalitsis et al., 2000; Lermontova et al., 2011b).
In most eukaryotes centromeres are confined to a restricted region on each chromosome that serves as a platform for assembly of the kinetochore, a multiprotein structure that connects chromosome with the spindle. Centromeres are defined epigenetically by a specialized centromeric histone H3 variant (CENH3), which forms the foundation for the recruitment of kinetochore proteins (McAinsh and Marston, 2022). In contrast to canonical histones, replicative dilution of CENH3 is not replenished immediately in S-phase, but in subsequent stages of the cell cycle (Stirpe and Heun, 2023). In plants, CENH3 is loaded on centromeres in G2 and persists there throughout the cell cycle (Talbert et al., 2002; Lermontova et al., 2006). CENH3 is a very stable component of centromeric chromatin, although its amount at centromeres gradually declines in terminally differentiated cells in plants and animals (Lermontova et al., 2011a; Swartz et al., 2019).
In this study, we have discovered that heat stress significantly reduces the amount of CENH3 on meiotic chromosomes in Arabidopsis thaliana. This loss of CENH3 leads to the formation of micronuclei, which in turn contributes to the decrease in pollen formation and fertility in plants exposed to heat stress. Additionally, we have found that plants with a genetic mutation that reduces the amount of centromeric histone are more sensitive to moderately elevated temperature. These results suggest that meiotic centromeres may represent a crucial point of vulnerability for plants in adaptation to raising temperatures.
Pollen production and fertility decline with increasing temperature
In our previous work, we identified the cenh3-4 allele of Arabidopsis centromeric histone CENH3 that carries a mutation in the splicing donor site of the 3rd exon (Capitao et al., 2021). This leads to a 10-fold reduction in fully spliced CENH3 mRNA and a decreased amount of centromeric histone. Consequently, cenh3-4 plants have smaller centromeres and are sensitive to oryzalin (Capitao et al., 2021). Under standard conditions, cenh3-4 mutants are barely distinguishable from wild type, but we noticed their reduced fertility when grown at an elevated temperature. To systematically assess this phenotype, we grew plants under standard conditions (21°C) until they formed four true leaves, and then continued their cultivation in growth chambers tempered to 16, 21, 26 and 30°C (Figure S1). At 16°C, plants exhibited the slowest growth, but also the highest fertility, as assessed by pollen count and silique length, which is indicative of seed yield (Figure 1). Fertility slightly decreased at 21°C and further declined at 26°C. In cenh3-4 mutants, we noticed a sharp decline in pollen production and fertility at 26°C (86±48 pollen per anther), whereas the fertility of the wild type was still relatively high (213±58 pollen per anther) (Figure 1B,C). Both cenh3-4 and wild type plants became infertile at 30°C. The heat-induced sterility was reversible and cenh3-4 as well as wild type plants transferred from 30 to 21°C regained fertile flowers (Figure S1C). These data indicate that pollen production and fertility gradually decline with increasing temperature and this trend is particularly pronounced in cenh3-4 mutants that have become almost sterile already at 26°C.
It has been reported that extreme heat stress alters chromosome segregation fidelity and the duration of Arabidopsis meiosis (De Jaeger-Braet et al., 2022). Temperatures of 34°C and above abolished chromosome pairing and synapsis and led to the formation of univalents. However, meiotic chromosomes are fully paired at 30°C (Ning et al., 2021; De Jaeger-Braet et al., 2022; Fu et al., 2022) indicating that the recombination defects are not primarily responsible for abortive pollen development at this temperature.
Heat stress delays meiotic progression and induces micronuclei
To assess the effect of temperature on meiotic progression and chromosome segregation at temperatures up to 30°C, we performed live imaging of meiotic divisions in the HTA10:RFP reporter line, which marks chromatin (Valuchova et al., 2020). We measured the duration of meiotic divisions from the end of diakinesis until the formation of haploid nuclei in telophase II (Figure S2). Meiotic divisions were slowest at 16°C and lasted, on average, 441 and 459 min in wild type and cenh3-4, respectively (Movies 1 and 2, Figure 2A). Meiosis progressed significantly faster with increasing temperature. Wild type meiotic divisions took 169 min at 21°C and 142 min at 26°C (Movies 3 and 4). Meiotic divisions were also rapid in cenh3-4 at 21°C, lasting on average 156 min, but slowed down to 238 min at 26°C (Movies 5 and 6). This contrasts with the situation in wild type, where divisions occur at the fastest rate at 26°C. Interestingly, meiotic divisions were prolonged at 30°C in both wild type and cenh3-4 mutants to 286 and 264 min, respectively (Movies 7 and 8). The slowdown of meiosis at 26°C and 30°C in cenh3-4, and at 30°C in wild type coincides with the steep decline of pollen production (Figure 1B,C) and indicates problems with meiotic progression.
Live imaging in cenh3-4 plants and, at 30°C, also in wild type showed formation of micronuclei that began to form during meiosis I and persisted till telophase II (Movies 7 and 8). We quantified the micronuclei in fixed anthers at the tetrad stage using confocal microscopy (Figure 2B,C). The micronuclei were apparent in cenh3-4 mutants at all temperatures, which is consistent with partially impaired centromere function in these plants (Capitao et al., 2021). Nevertheless, their occurrence substantially increased at 26°C and severe defects in chromosome segregation were observed at 30°C (Figure 2B,C; Movies 6 and 8) resulting in very few regular tetrads. Whereas we occasionally detected micronuclei at lower temperatures also in wild type, albeit at a much lower level than in cenh3-4, their incidence increased 15-fold between 26°C and 30°C (Figure 2B,C, Movies 4 and 7). In addition, we observed dyads and polyads, which is consistent with the previous study performed at a similar temperature (De Storme and Geelen, 2020). These data show that temperature-induced fertility reduction coincides with increased occurrence of micronuclei.
Micronuclei may arise from acentric fragments and dicentric chromosomes derived from defective repair of meiotic breaks. Indeed, temperatures above 30°C were reported to cause aberrant recombination intermediates and induce pachytene checkpoint (De Storme and Geelen, 2020; De Jaeger-Braet et al., 2022). In this scenario, the absence of meiotic breaks in SPO11-deficient plants should prevent the formation of temperature-induced micronuclei. Arabidopsis spo11-2 mutants do not form bivalents, and homologous chromosomes segregate randomly in anaphase I (Stacey et al., 2006; Hartung et al., 2007). Cytogenetic analysis in spo11-2 plants revealed a relatively high level of micronuclei in tetrads at 21°C (Figure 2B,C). Because unpaired univalents cannot properly bi-orient at metaphase I spindle, these micronuclei are likely generated by chromosome mis-segregation. Importantly, number of the micronuclei almost doubled in spo11-2 plants grown at 30°C (Figure 2B,C) arguing that temperature has a direct effect on chromosome segregation.
In its natural habitats, A. thaliana usually flowers from March to early summer under average daily temperatures lower than the ones used for cultivation in laboratories (Shindo et al., 2007; Brachi et al., 2010). Due to the day-night cycle, they never experience sustained temperatures over 30°C. Therefore, we tested whether meiotic defects observed after continuous cultivation at 30°C could also be induced under more physiological conditions that mimic a hot day. To this end, we cultivated plants in chambers tempered to 18°C overnight and increased temperature to 34°C for 6 hrs during the day (Figure S3A). We observed a drastic reduction in fertility and pollen count, as well as an increased frequency of micronuclei compared to control plants grown under the 18°C/21°C night/day regime (Figure S3B-D). These data suggest that heatwaves occurring during flowering can have a detrimental effect on Arabidopsis meiosis.
Heat stress reduces the amounts of centromeric histone on meiotic centromeres
Our phenotypic analysis indicates that cenh3-4 mutants grown at 26°C exhibit the same behavior as wild type plants grown at 30°C. Therefore, we investigated whether increasing temperature weakens the centromere structure, which could explain the temperature sensitivity of cenh3-4 plants with less centromeric histone. CENH3 is present on meiotic chromosomes from early prophase I (Talbert et al., 2002; Lermontova et al., 2006) and its signal is most visible in pachytene when homologous chromosomes are fully synapsed. To evaluate the effect of temperature on centromeres, we analyzed the CENH3 signal on pachytene chromosomes in anthers of the Arabidopsis eYFP:CENH3 reporter line (Le Goff et al., 2020; Demidov et al., 2022) grown at 21, 26 and 30°C. While eYFP:CENH3 was easily detectable on pachytene chromosomes at 21°C, the signal decreased significantly at 26°C and declined further at 30°C (Figure 3A,B). We observed this trend in three independent experiments (Figure 3B). In contrast, the eYFP:CENH3 signal in tapetum nuclei neighboring the pollen mother cells exhibited the opposite trend and increased with elevated temperature (Figure 3A,C). This resulted in a striking difference in signal intensity at 30°C, where the tapetum exhibited a strong eYFP:CENH3 signal, whereas the signal on pachytene chromosomes in the same field of view was barely detectable (Figure 3A).
CENH3 acts as a platform for binding additional kinetochore and spindle assembly checkpoint proteins. BMF1 is an Arabidopsis BUB1-related protein that associates with centromeres throughout the cell cycle (Komaki and Schnittger, 2017). We generated Arabidopsis marker lines expressing BMF1::BMF1:eYFP and BMF1::BMF1:TagRFP constructs, and validated BMF1 co-localization with CENH3 from early prophase I until telophase II (Figure S4, Movie 9). Quantification of the BMF1:eYFP signal on pachytene chromosomes showed a decline at 26°C, and BMF1 was undetectable at 30°C (Figure 3D,E). These data suggest that elevated temperature reduces the amount of CENH3 and the kinetochore protein BMF1 on meiotic centromeres, potentially affecting their functionality.
Heat stress prolongs spindle assembly checkpoint in metaphase I
In our previous report, we showed that reduced amount of CENH3 and smaller centromeres prolonged the biorientation of mitotic chromosomes in cenh3-4 plants (Capitao et al., 2021). Biorientation is monitored by the spindle assembly checkpoint (SAC), and its satisfaction triggers anaphase. We hypothesized that meiotic chromosomes with heat-induced reductions in CENH3 might take longer to properly attach to the spindle and satisfy the SAC. The core SAC proteins temporarily associate with the kinetochore during spindle formation and disappear shortly before the onset of anaphase. BMF3 is one of the core components of the Arabidopsis SAC that associates with the kinetochore during prometaphase and extends this association in the presence of a spindle inhibitor (Komaki and Schnittger, 2017). To monitor SAC on meiotic chromosomes, we generated an Arabidopsis line expressing BMF3::BMF3:GFP together with the tubulin marker TagRFP:TUB4. Initially, we validated the localization of BMF3 on meiotic chromosomes by live cell imaging since BMF3 was previously only analyzed in the context of mitosis (Komaki and Schnittger, 2017). We observed the BMF3:GFP signal during prometaphase/metaphase I, it disappeared at the onset of anaphase I and reappeared again during metaphase II indicating that BMF3 is a component of the meiotic SAC (Figure 4A, Movie 10). We then analyzed the effect of temperature on SAC duration. Since the signal was more prominent in metaphase I, we measured the duration of the active SAC during meiosis I. We observed that while the BMF3:GFP signal persisted, on average, for about 22.7 min at 21 and 26°C, its appearance was prolonged to 40.5 min at 30°C (Figure 4B, Movies 11 and 12). This observation supports our finding that centromere structure is compromised at elevated temperatures, which may extend the time necessary for chromosome biorientation.
In this study we investigated the impact of increased temperature on fertility and meiotic progression in Arabidopsis. We observed that A. thaliana (ecotype Col-0) exhibited the highest fertility when grown at 16°C, which closely resembles the average temperature during flowering in its natural habitats (Brachi et al., 2010). At this temperature, meiotic divisions took more than twice as long as at 21°C. Arabidopsis grown at 17°C was reported to exhibit slower microsporogenesis, with the entire meiosis process lasting 48 hours as opposed to 32 hours at 24°C (Zhu et al., 2020). Interestingly, the slower progression was found to be less detrimental for several mutations that affect microsporogenesis, indicating that a slower pace provides greater robustness. Overall, these observations suggest that the pace of meiosis in Arabidopsis is well optimized for its natural growth conditions, enabling the plants to achieve maximum fitness.
The process of meiosis is progressively accelerated with increasing temperature (De Jaeger-Braet et al., 2022) (Figure 2). However, the speed of meiotic divisions reaches its maximum at 26°C and slows down at 30°C under the conditions used in this study. At 30°C, fertility is lost and high incidence of micronuclei is observed, implying that the temperature around 30°C impairs some molecular processes involved in chromosome segregation and genome integrity. The micronuclei can represent acentric fragments derived from aberrant processing of recombination intermediates. Indeed, the impact of temperature on recombination machinery is well documented. A moderate increase in temperature leads to shift in chiasma distribution and cross-over frequency (Higgins et al., 2012; Lloyd et al., 2018; Modliszewski et al., 2018). Heat stress above 30°C severely impairs recombination and synapsis, leading to univalents and chromosome segregation defects (De Storme and Geelen, 2020; Ning et al., 2021; De Jaeger-Braet et al., 2022; Fu et al., 2022). Nevertheless, we observed an increased incidence of micronuclei also in SPO11-deficient plants that do not form meiotic DNA double-strand breaks indicating that only a portion of micronuclei is derived from aberrant recombination.
Micronuclei can also be formed by aberrant chromosome segregation during anaphase (Fenech et al., 2011). The accurate partitioning of chromosomes depends on the correct attachment of centromeres to the spindle microtubules. Recent reports have indicated that the stability of microtubules in plant meiocytes is compromised by heat stress (Wang et al., 2017; De Jaeger-Braet et al., 2022). In this study, we have demonstrated that high temperature also affects the structure and function of meiotic centromeres. Our findings suggest that elevated temperature reduces the amount of CENH3 and BMF1 on meiotic centromeres (Figure 3). These weakened centromeres may be less effective in establishing productive interactions with spindle microtubules. This is consistent with the prolonged spindle assembly checkpoint we observed through the longer residency of BMF3 on prometaphase I centromeres at 30°C (Figure 4). Taken together, our data indicate that heat stress impairs centromere function, which in turn decreases the efficiency of proper attachment of chromosomes to the meiotic spindle. As a result, these chromosomes may not be transported to their intended destination in a timely manner and, therefore, not incorporated into the newly formed nuclei. This scenario also explains the increased temperature-sensitivity of cenh3-4 plants, which already have smaller centromeres (Capitao et al., 2021); even moderately elevated temperature may further enhance this defect.
How elevated temperature affects the amount of CENH3 on centromeres? Studies in human cells have revealed that CENH3 nucleosomes are less stable than previously thought. The amount of CENH3 at centromeres declines over time and must be actively replenished to preserve centromere identity and proliferative potential (Swartz et al., 2019). Transcription has been shown to cause the eviction of pre-existing CENH3 from the centromeres (Swartz et al., 2019). In Arabidopsis, depletion of CENH3 from centromeres has been observed in leaf cells with increasing age, indicating that this phenomenon also occurs in plants (Lermontova et al., 2011a). The Arabidopsis centromeric satellite repeat CEN180 undergoes pervasive transcription, which is largely repressed by epigenetic silencing mechanisms (May et al., 2005). However, heat stress can alleviate the repression and induce transcription of CEN180 from silent loci (Tittel-Elmer et al., 2010). This temperature-induced transcription can increase the eviction of CENH3 nucleosomes, potentially compromising centromere structure if not replenished. Interestingly, in contrast to pollen mother cells, tapetum cells do not exhibit CENH3 loss at elevated temperatures (Figure 3C). This may be due to the different efficiency of CENH3 deposition to replenish the heat-induced loss of CENH3 in these cell types. Accordingly, Arabidopsis meiotic cells possess a specialized CENH3 loading mechanism that seems more stringent than the loading mechanism operating in mitotic cells (Lermontova et al., 2011b; Ravi et al., 2011; Schubert et al., 2014).
Crosses between wild type and plants with altered CENH3 can result in postzygotic loss of one set of parental chromosomes (Ravi and Chan, 2010). This is attributed to inefficient re-loading of CENH3 on the set of parental chromosomes with the altered centromeres in hybrid embryos (Marimuthu et al., 2021). Recent studies showed that heat stress applied during early embryogenesis could enhance the centromere-mediated genome elimination in Arabidopsis (Ahmadli et al., 2022; Jin et al., 2023; Wang et al., 2023). This indicates that similarly to meiocytes, elevated temperature can also destabilize centromeres in embryonic cells.
In conclusion, our study highlights the significant impact of temperature on the centromere structure and reproduction in Arabidopsis. We found that already moderate increase in temperature has a discernable effect on pollen production and silique length, and exposure to 30°C impairs the structure of centromeres and leads to plant sterility. These findings are particularly relevant in the context of climate change, where rising temperatures and more frequent weather extreme periods pose a threat to global food security by disrupting plant reproductive processes. As such, our study provides important insights into the mechanisms that contribute to the reduction of plant fertility in response to elevated temperature. Further research on molecular aspects underlying these effects may help to develop strategies to generate plants more resilient to extreme weather during their reproductive phase.
Material and Methods
Plant material and growth conditions
Arabidopsis thaliana ecotype Columbia (Col-0), cenh3-4 (Capitao et al., 2021) and spo11-2-3 (Hartung et al., 2007) seeds were grown on soil in growth chambers at 21°C, 16 h/8 h light/dark cycles and 50% of humidity until the 1.04 growth stage. Plants were then transferred to different chambers with continuous 16, 21, 26, 30°C or 34°C/18°C and 21°C/18°C at 16 h/8 h light/dark cycles and 50% of humidity. Plants used for live cell imaging were generated by crossing HTA10:RFP (Valuchova et al., 2020) and pRPS5A::TagRFP:TUB4 (Prusicki et al., 2019) reporter lines with cenh3-4 mutant and BMF3::BMF3:GFP reporter line, respectively. eYFP:CENH3 reporter line was described and characterized in previous studies (Le Goff et al., 2020; Demidov et al., 2022).
Generating reporter lines
To generate the BMF1 and BMF3 reporter lines, we amplified the promoter and genomic regions of BMF1 using the primers CACCTGAGTCTCCAACGTTA and CGAAGAGCATAACGAGATGCG. The PCR products were then cloned into the destination vectors pGWB659 and pGWB640, respectively, to create marker lines tagged with TagRFP and eYFP at the C-terminus. Similarly, we amplified the BMF3 promoter and genomic regions using the primers CACCATGCAGATGGTCCTCC and GAAGTCCATTGGCATTGCAAA, and subcloned them into the pGWB650 destination vector using gateway cloning to generate the BMF3::BMF3:GFP line. We then introduced these constructs into wild-type plants using Agrobacterium-mediated floral dip transformation. We used non-segregating homozygous transgenic lines for microscopic analyses.
Assessment of plant fertility
Pollen viability was determined by Alexander staining (Alexander, 1969). Anthers were imaged using Zeiss Axioscope A1 equipped with 20x/0.5 objective (Zeiss) and processed in Zeiss ZEN software. Silique length was assessed from images of main stems scanned by an Epson scanner and the siliques were measured using the Fiji Analyze/Measure function (Schindelin et al., 2012).
To examine micronuclei, DAPI staining of PMCs was used in whole anther as described (Capitao et al., 2021). Anthers were imaged using Zeiss LSM780 confocal microscope (63X/1.4 oil objective) and image processing was done using Zeiss ZEN software (Zeiss). To quantify the fluorescence signal intensity of eYFP:CENH3 and BMF1:eYFP, anthers were DAPI stained as described above, and Z-stacks were acquired using Zeiss LSM880 confocal microscope equipped with the Fast module 32-channels Airyscan detector (63x/1.4 oil objective). The fluorescence intensity was quantified using Fiji (Schindelin et al., 2012) according to the procedure described by Shihan et al. (Shihan et al., 2021). This involved generating a SUM of signal in z-stacks covering one nucleus, background subtraction, and measuring Raw Integral Density per one signal. This process was repeated individually in each nucleus.
Live cell imaging
Live cell imaging of meiosis was performed by light-sheet fluorescent microscopy using Light-sheet Z.1 microscope (Zeiss) as previously described (Valuchova et al., 2020; Capitao et al., 2021). Imaging of male meiosis was conducted using 10x or 20x objectives (Detection optics 10x/0.5 or 20x/1.0), single illumination (Illumination Optics 10x/0.2) and two track imaging with 488 nm laser for GFP and eYFP, and 561 nm laser for RFP, in 5 min time increments. Imaging of BMF3:GFP and TagRFP:TUB4 markers for SAC analysis was performed using fast scanning in 1 min time increments. Images were deconvolved with a Regularized Inverse Filter and further processed in Zeiss ZEN software for Light-sheet (Zeiss). To correct occasional sample drift, the Correct 3D drift plugin in Fiji (Parslow et al., 2014) was used.
We acknowledge the support from CEITEC MU Core facilities Plant Sciences and CELLIM, supported by the Czech-Bioimaging (No. LM2018129) infrastructure project funded by MEYS CZ. This work was funded by the Czech Science Foundation (grant 21-25163J to K.R.). MK is supported by Deutsche Forschungsgemeinschaft (LE2299/5–1).
Movie 1. Live imaging of meiosis in HTA10:RFP chromatin marker in plants grown at 16°C.
Movie 2. Live imaging of meiosis in cenh3-4 plants carrying HTA10:RFP chromatin marker grown at 16°C. Production of micronuclei could be seen in a few PMCs.
Movie 3. Live imaging of meiosis in HTA10:RFP chromatin marker in plants grown at 21°C.
Movie 4. Live imaging of meiosis in HTA10:RFP chromatin marker in plants grown at 26°C.
Movie 5. Live imaging of meiosis in cenh3-4 plants carrying HTA10:RFP chromatin marker grown at 21°C. Production of micronuclei could be detected in some PMCs.
Movie 6. Live imaging of meiosis in cenh3-4 plants carrying HTA10:RFP chromatin marker grown at 26°C. Production of micronuclei could be detected in most PMCs.
Movie 7. Live imaging of meiosis in HTA10:RFP chromatin marker in plants grown at 30°C. Aberrant meiotic products are detected.
Movie 8. Live imaging of meiosis in cenh3-4 plants carrying HTA10:RFP chromatin marker grown at 30°C. Most PMCs undergo aberrant meiotic division and only a few PMCs undergo normal meiotic division resulting in forming unbalanced tetrads.
Movie 9. Live imaging of BMF1:eYFP kinetochore marker and HTA10:RFP chromatin marker during meiosis.
Movie 10. Live imaging of BMF3:GFP spindle assembly checkpoint marker and TagRFP:TUB4 tubulin marker during both meiosis.
Movie 11. Live imaging of BMF3:GFP spindle assembly checkpoint marker and TagRFP:TUB4 tubulin marker during first meiotic division of plants grown at 26°C.
Movie 12. Live imaging of BMF3:GFP spindle assembly checkpoint marker and TagRFP:TUB4 tubulin marker during the first meiotic division of plants grown at 30°C.
- High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in ArabidopsisPlant Commun
- Differential Staining of Aborted and Nonaborted PollenStain Technol 44
- A meiotic time-course for Arabidopsis thalianaSex Plant Reprod 16:141–149
- Linkage and association mapping of Arabidopsis thaliana flowering time in naturePLoS Genet 6
- Severity of drought and heatwave crop losses tripled over the last five decades in EuropeEnviron Res Lett 16
- A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient ArabidopsisPLoS Genet 17
- Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thalianaPlant Cell 34:433–454
- The impact of environmental stress on male reproductive development in plants: biological processes and molecular mechanismsPlant Cell Environ 37:1–18
- High temperatures alter cross-over distribution and induce male meiotic restitution in Arabidopsis thalianaCommun Biol 3
- Haploid induction by nanobody-targeted ubiquitin-proteasome-based degradation of EYFP-tagged CENH3 in Arabidopsis thalianaJ Exp Bot 73:7243–7254
- Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cellsMutagenesis 26:125–132
- Interfered chromosome pairing at high temperature promotes meiotic instability in autotetraploid ArabidopsisPlant Physiol 188:1210–1228
- The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in ArabidopsisPlant Cell 19:3090–3099
- Acute heat stress during stamen development affects both the germline and sporophytic lineages in Arabidopsis thaliana (L.) HeynhEnviron Exp Bot 173
- Spatiotemporal asymmetry of the meiotic program underlies the predominantly distal distribution of meiotic crossovers in barleyPlant Cell 24:4096–4109
- Heat stress response mechanisms in pollen developmentNew Phytol 231:571–585
- Heat stress promotes haploid formation during CENH3-mediated genome elimination in ArabidopsisPlant Reproduction
- Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesisGenes Dev 14:2277–2282
- Arabidopsis HEAT SHOCK FACTOR BINDING PROTEIN is required to limit meiotic crossovers and HEI10 transcriptionEMBO J 41
- The Spindle Assembly Checkpoint in Arabidopsis Is Rapidly Shut Off during Severe StressDev Cell 43:172–185
- The H3 histone chaperone NASP(SIM3) escorts CenH3 in ArabidopsisPlant J 101:71–86
- Deposition, turnover, and release of CENH3 at Arabidopsis centromeresChromosoma 120:633–640
- Loading of Arabidopsis centromeric histone CENH3 occurs mainly during G2 and requires the presence of the histone fold domainPlant Cell 18:2443–2451
- Knockdown of CENH3 in Arabidopsis reduces mitotic divisions and causes sterility by disturbed meiotic chromosome segregationPlant J 68:40–50
- Development of Wild and Cultivated Plants under Global Warming ConditionsCurr Biol 29:R1326–R1338
- Plasticity of Meiotic Recombination Rates in Response to Temperature in ArabidopsisGenetics 208:1409–1420
- Effects of elevated temperature on meiotic chromosome synapsis in Allium ursinumChromosoma 97:449–458
- Epigenetically mismatched parental centromeres trigger genome elimination in hybridsSci Adv 7
- Differential regulation of strand-specific transcripts from Arabidopsis centromeric satellite repeatsPLoS Genet 1
- The Four Causes: The Functional Architecture of Centromeres and KinetochoresAnnu Rev Genet 56:279–314
- Elevated temperature increases meiotic crossover frequency via the interfering (Type I) pathway in Arabidopsis thalianaPLoS Genet 14
- Heat stress interferes with formation of double-strand breaks and homolog synapsisPlant Physiol 185:1783–1797
- Sample drift correction following 4D confocal time-lapse imagingJ Vis Exp
- Polyploidization mechanisms: temperature environment can induce diploid gamete formation in Rosa spJournal of Experimental Botany 62:3587–3597
- Live cell imaging of meiosis in Arabidopsis thalianaElife 8
- Haploid plants produced by centromere-mediated genome eliminationNature 464:615–618
- Meiosis-specific loading of the centromere-specific histone CENH3 in Arabidopsis thalianaPLoS Genet 7
- A simple method for quantitating confocal fluorescent imagesBiochem Biophys Rep 25
- Natural genetic variation in Arabidopsis: tools, traits and prospects for evolutionary ecologyAnn Bot 99:1043–1054
- Fiji: an open-source platform for biological-image analysisNat Methods 9:676–682
- Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?Chromosoma 123:491–497
- Arabidopsis SPO11-2 functions with SPO11-1 in meiotic recombinationPlant J 48:206–216
- The ins and outs of CENP-A: Chromatin dynamics of the centromere-specific histoneSemin Cell Dev Biol 135:24–34
- Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative PotentialDev Cell 51:35–48
- Centromeric localization and adaptive evolution of an Arabidopsis histone H3 variantPlant Cell 14:1053–1066
- Stress-induced activation of heterochromatic transcriptionPLoS Genet 6
- Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopyElife 9
- High temperature-induced production of unreduced pollen and its cytological effects in PopulusSci Rep 7
- A simple and highly efficient strategy to induce both paternal and maternal haploids through temperature manipulationNat Plants 9:699–705
- Slowing development restores the fertility of thermo-sensitive male-sterile plant linesNat Plants 6:360–367
- Temperature stress and plant sexual reproduction: uncovering the weakest linksJ Exp Bot 61:1959–1968