Effect of temperature on meiosis duration and micronuclei formation.
(A) Graphical representation of the duration of Meiosis I (from the end of diakinesis to the end of anaphase I; Figure S2), Interkinesis, and Meiosis II (prometaphase II to telophase II) calculated from live imaging of anthers in wild type (WT) and cenh3-4 plants grown at 16, 21, 26 and 30°C. Error bars represent standard deviation (from 16 to 30°C: in wild type n=36,36, 36,46 and cenh3-4 n=35,24,24,4, resp.) Significance of the difference is indicated (two tailed t-test; **** p<0.0001). (B) Anther loculi in the tetrad stage of wild type, cenh3-4 and spo11-2-3 plants grown at 16, 21, 26 and 30°C. DNA was stained with DAPI. Blue arrowheads indicate examples of produced micronuclei. Scale bar=10 µm. (C) Number of micronuclei per lobe in wild type (WT, n=19,19,19,19), cenh3-4 (n=19,19,19,19), and spo11-2-3 (n=19,26,21) plants. Error bars represent standard deviation. Significance of the difference from plants of the corresponding genotype grown at 21°C is indicated (two tailed t-test; * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001).