Hierarchical cluster analysis on microglia enriche genes among hiPSC-derived microglial cells (hiPSC-MG) and human adult brain microglia cells (AMG), fetal brain microglia cells (FMG), inflammatory monocytes (IM), monocytes(M). The human microglia gene panel combined our mouse microglia-enriched genes and human microglia-enriched genes (Abud et al., 2017; Muffat et al., 2016; Douvaras et al., 2017; Böttcher et al., 2019; Van et al., 2019). The total microglia enriched gene list contains 203 genes (Suppl. Table 1), which used to be extracted from the gene profile of hiPSC-MG and downloaded human adult microglia(AMG), fetal microglia (FMG), inflammatory monocyte (IM) and monocytes (M) (GSE 178846, Abud et al., 2017). 188 genes (Suppl. table 1) were obtained from both gene profiles. All the gene counts were normalized with 4 human cell housekeeping genes C1orf43, RAB7A, REEP5 and VCP (Eisenberg et al., 2013). The hierarchical cluster was analyzed by JMP (JMP Statistical Discovery LLC.). Results showed that hiPSC-MG is more comparable with AMG and FMG.