Differentiation and characterization of human iPSC-derived microglia cell. (A) Human iPSC were cultured in a 6-well plate. Scale bar = 200µm. (B) Embryoid bodies formation in AggreWell™800 plate at day eight in culture medium mTeSR1 plus BMP4, VEGF, and SCF. Scale bar = 200µm. (C) Myeloid precursor cluster at the 1-month culture of embryoid bodies under TheraPEAK™ X-vivo™-15 Serum-free Hematopoietic Cell Medium with M-CSF and IL3. Scale bar = 50µm. (D) Microglia cells maturation culture for two weeks with DMEM/F12 plus nonessential amino acids, glutamine, IL34, CSF1, TGFb2, and CX3CL1. Scale bar = 50µm. (E) Immunocytochemistry staining with Iba1 and human CD34, CX3CR1, P2ry12, CD11b, CD68. Scale bar = 100µm. (F) The cell counts and collaboration analysis of CD34 and Iba1 positive cells. (G) Percentage analysis of myeloid cell marker CX3CR1, CD11b, activation marker CD68, and microglia cell signature marker P2ry12.