Peer review process
Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the editors and peer reviewers.
Read more about eLife’s peer review process.Editors
- Reviewing EditorBavesh KanaUniversity of the Witwatersrand, Johannesburg, South Africa
- Senior EditorBavesh KanaUniversity of the Witwatersrand, Johannesburg, South Africa
Reviewer #1 (Public review):
The authors present data on outer membrane vesicle (OMV) production in different mutants, but they state that this is beyond the scope of the current manuscript, which I disagree with. This data could provide valuable physiological context that is otherwise lacking. The preliminary blots suggest that YafK does not alter OMV biogenesis. I recommend repeating these blots with appropriate controls, such as blotting for proteins in the culture media, an IM protein, periplasmic protein and an OM protein to strengthen the reliability of these findings. Including this data in the manuscript, even if it does not directly support the initial hypothesis, would enhance the physiological relevance of the study. Currently, the manuscript relies completely on the experimental setup (labeling-mass spec) previously developed by the authors, which limits the broader scope and interpretability of this study.
Additionally susceptibility of strains to detergents like SDS can be tested to provide a much needed physisological context to the study.
In summary, the authors should consider revising the manuscript to improve clarity, substantiate their claims with more detailed evidence, and include additional experimental results that provide necessary physiological context to their study.
Comments on the revised version:
Regarding my comments from last review on a new figure on OMV analysis, The authors have redirected me to their previous response and have not performed the suggested control blots. I do not get their argument that this is for specialized audience. I do not have any more comments.