Introduction

Orexin neurons are exclusively located in the lateral hypothalamus (LH) and adjacent regions in the brain, including the perifornical area, and dorsomedial and posterior hypothalamus (Soya & Sakurai, 2020). Abundant orexin fibers innervate the ventral tegmental area (VTA) and substantia nigra (SN), which together contain the majority of dopaminergic neurons in humans and rodents (Fadel & Deutch, 2002; Hrabovszky et al., 2013; Peyron et al., 1998). There are also some dopaminergic neurons in other brain regions, although not all of these neurons express the dopamine transporter (DAT) (Koblinger et al., 2018; Sharples et al., 2014; Turiault et al., 2007). Both the orexin and dopaminergic systems control food intake, locomotor activity, reward processing as well as energy metabolism (Howe & Dombeck, 2016; Inutsuka & Yamanaka, 2013; Narayanan et al., 2010; Palmiter, 2007; Tsujino & Sakurai, 2013). Dopamine was discovered as a neuro-active molecule approximately 70 years ago and the dopaminergic system has diverse functions (Iversen & Iversen, 2007). In a simplified model the nigrostriatal dopaminergic system, containing the dopaminergic projections from SN to the dorsal striatum, predominantly regulates action selection and exploratory behaviors. In contrast, the mesolimbic and mesocortical dopaminergic systems, which consist of the dopaminergic pathways from the VTA to the ventral striatum and prefrontal cortex, predominantly regulate motivation, cognition, decision making, reward, and aversive behavior (Kutlu et al., 2021; Latif et al., 2021; Lerner et al., 2021; Roeper, 2013). Some VTA dopaminergic neurons also represent movements in trained animals (Lerner et al., 2021). Dopaminergic neurons modulate movement differently via direct and indirect pathways through the striatum (Kim et al., 2017; Kreitzer & Malenka, 2008; Roseberry et al., 2016). Co-release of other neuro-active molecules by dopaminergic terminals (Barcomb & Ford, 2023; Buck et al., 2021; Zych & Ford, 2022) and the spatial and temporal dynamics of dopamine release (Berke, 2018; Chantranupong et al., 2023; Liu et al., 2021) could further affect the precision and diversity of dopaminergic functions.

Orexin A injection into the VTA increases cocaine-and morphine-related reward processing (Mahler et al., 2012), and intra-SN injection of orexin A induces hyperlocomotion and stereotypic behaviors (Liu et al., 2018; Nakamura et al., 2000). On the other hand, systemic injection of dopamine receptor antagonists reduces orexin A-induced increase of hyperlocomotion, stereotypic and grooming behaviors (Nakamura et al., 2000). However, whether the above effects of orexin signaling in the VTA and SN are mediated by dopaminergic or non-dopaminergic cells is not yet clear.

The orexin system consists of the two peptides orexin A and B, which are derived from the precursor, prepro-orexin (de Lecea et al., 1998; Sakurai et al., 1998). Both are endogenous ligands for two G-protein-coupled receptors, orexin receptor subtype 1 (Ox1R), and 2 (Ox2R) (Sakurai et al., 1998). Orexin A activates both receptors, while orexin B predominantly activates Ox2R (Sakurai et al., 1998). The mRNA detection by in situ hybridization revealed the expression of both receptors in the VTA and SN (Marcus et al., 2001). However, the cell-type specific expression of Ox1R and Ox2R in the VTA and SN is not well understood. Electrophysiological studies showed that orexin receptors are present in dopaminergic VTA neurons (Baimel et al., 2017; Korotkova et al., 2003; Tung et al., 2016), whereas data for the SN seem controversial (Korotkova et al., 2002; Liu et al., 2018).

Here, we aim to map the expression patterns of Ox1R and Ox2R in dopaminergic VTA and SN neurons and investigate the functional role of Ox1R using conditional knockout mice.

Results

Ox1R is the predominant orexin receptor in dopaminergic neurons

To analyze the expression patterns of orexin receptors in dopaminergic VTA and SN neurons, we performed fluorescent in situ hybridization (RNAscope) of tyrosine hydroxylase (Th), Ox1R (Hcrtr1) and Ox2R (Hcrtr2) in control (Ox1R flox/flox, DAT wt/wt) mice and Ox1R^ΔDAT (Ox1R fl/fl, DAT-Cre tg/wt) mice, from the same breeding crossing mice carrying a loxP-flanked Ox1R-allele (Ox1R fl/fl) with DAT-Cre mice (Ekstrand et al., 2007).

In control mice, Ox1R was expressed in 44 -58% of dopaminergic neurons in all VTA and SN sub-regions, including the interfascicular (IF), paranigral (PN), parainterfascicular (PIF) and parabrachial (PBP) subnuclei of the VTA and SN. In contrast, Ox2R-positive dopaminergic neurons were mainly located in the medial VTA, including IF, PN, and PIF, with 25 - 42% of dopaminergic neurons expressing the Ox2R (Figure 1A, C, D, G-I; Figure 1—figure supplement 1A). While the above data were calculated as the mean percentages in the investigated mice, we also calculated the mean dopaminergic neuron numbers separately for those expressing both or exclusively one of the orexin receptor types. Thus, we could analyze the distribution of Ox1R-, Ox2R-, Ox1R and Ox2R-, and none (of orexin receptors)-expressing dopaminergic neurons in each VTA subregion and the SN (Figure 1J). The results were similar to the above: 47 - 59% of dopaminergic neurons expressed Ox1R in all VTA and SN sub-regions, and Ox2R was mainly expressed by 26 - 41% of dopaminergic neurons in the medial VTA (Figure 1J). 19 - 25% of dopaminergic neurons in the medial VTA expressed both Ox1R and Ox2R (Figure 1J). Of note, abundant Ox1R and Ox2R expression was present in Th-negative neurons in the VTA, SN, and neighboring regions (Figure 1A, C, D, Figure 1—figure supplement 1A).

Figure 1

Since most dopaminergic neurons express Ox1R, we focused the functional analysis on Ox1R signaling in dopaminergic neurons. Therefore, Ox1R was specifically inactivated in dopaminergic (DAT-expressing) neurons of mice (Ox1R^ΔDAT). As a prerequisite for the functional analysis (behavior and PET imaging) validation of the Ox1R deletion was necessary. In Ox1R^ΔDAT mice, Ox1R expression was significantly reduced in dopaminergic neurons and remained unchanged in Th-negative cells (Figure 1B, E-I; Figure 1—figure supplement 1B). In contrast, the Ox2R expression patterns remained similar to that in the control mice, indicating the successful and specific knockout of Ox1R in dopaminergic neurons (Figure 1B, E-I; Figure 1—figure supplement 1B).

Next, we used perforated patch clamp recordings to establish that orexin A activates dopaminergic neurons not only in the mouse VTA (e.g., Baimel et al., 2017; Korotkova et al., 2003; Tung et al., 2016), but also in the mouse SN (Figure 1—figure supplement 2). In dopaminergic SN neurons that were pharmacologically isolated from GABAerigc and glutamatergic synaptic input 100 nM orexin increased the action potential frequency in 75% (6 of 8) of the neurons. Increasing the orexin A concentration to 300 nM further increased the firing frequency. This finding is consistent with previous extracellular recordings in rats (Liu et al. 2018). To also validate the Ox1R knockout in dopaminergic neurons functionally, we performed Ca²⁺ imaging in acute brain slices from control and Ox1R^ΔDAT mice. We used the genetically encoded Ca²⁺ indicator GCaMP6 to monitor [Ca²⁺]_i specifically in dopaminergic (DAT-expressing) neurons. This analysis focused on dopaminergic neurons in the SN, which we used as an "indicator population" because a large number of these neurons express Ox1R. During the experiments, GABAergic and glutamatergic synaptic input was blocked. Bath-applied 100 nM orexin A markedly and significantly increased [Ca²⁺]_i in dopaminergic SN control neurons (Figure 1I, K, Figure 1—figure supplement 3). Increasing the orexin A concentration to 300 nM further increased [Ca²⁺]_i (Figure 1I, K, Figure 1—figure supplement 3). For quantification, we tested each neuron whether it responded to 100 nM orexin A. A neuron was considered orexin-responsive if the change in GCaMP fluorescence induced by orexin A was three times larger than the baseline fluorescence’s standard deviation (3 σ criterion ≙ Z-score of 3). We found that 56% of the neurons tested responded to orexin A, while 44% of the neurons did not respond to orexin A (Figure 1L). These data are in very good agreement with the number of Ox1R-expressing neurons (Figure 1J). To determine the "population response" of all analyzed neurons, we measured the orexin-induced GCaMP6 fluorescence of each neuron expressed as a percentage of the GCaMP6 fluorescence induced by applying high (40 mM) K⁺ saline (Figure 1K, Figure 1—figure supplement 3). This is a solid reference point since it reflects the GCaMP6 fluorescence at maximal voltage-activated Ca²⁺ influx. The "population response" of all analyzed neurons was expressed as the mean ± SEM of these responses (Figure 1L). The significance of this mean response was tested for each group (control and Ox1R^ΔDAT) using a one-sample t-test. We found a highly significant and marked response of control neurons to 100 nM orexin A, while the Ox1R knockout neurons did not respond (control: p < 0.0001, n = 71; Ox1R^ΔDAT: p = 0.5, n = 86; one sample t-test for each group) (Figure 1L).

In conclusion, Ox1R is the main orexin receptor expressed in dopaminergic VTA and SN neurons and mediates their activation by orexin. Therefore, we wanted to determine further the functional role of Ox1R signaling in dopaminergic neurons. By comparing control and Ox1R^ΔDAT mouse data, we first identified behaviors modulated by orexin via dopaminergic neurons. In a second step, we used PET imaging to define and map neuronal pathways modulated by orexin.

Ox1R in dopaminergic neurons regulates novelty-induced locomotion and exploration

The behavioral test included an analysis of locomotor behavior, novelty exploration, reward processing, anxiety, and energy homeostasis. Since sex differences exist in orexin-and dopamine-mediated behaviors (Durairaja & Fendt, 2020; Freeman et al., 2021; Lippert et al., 2020; Zachry et al., 2021), we analyzed behavioral phenotypes in both males and females. Compared to controls, Ox1R^ΔDAT mice did not show significant changes in spontaneous locomotor activity in home cages (Figure 2—figure supplement 1). When exposed to a novel open field, male and female Ox1R^ΔDAT mice exhibited an increase in locomotion and exploration, with increased traveling distance, ambulating time, and vertical activity in the open field test (Figure 2A-C, E-G). Female Ox1R^ΔDAT mice also showed increased stereotypic activity (Figure 2D, H). Furthermore, intracerebroventricular (ICV) injection of orexin A (1 nmol) induced a more pronounced increase of locomotor activity in both female and male Ox1R^ΔDAT mice, compared to control mice (Figure 2I-L).

Figure 2

In a hole-board test, female Ox1R^ΔDAT mice showed increased nose pokes into the holes in early (1st and 2nd) sessions compared to control mice (Figure 2—figure supplement 2B). This effect disappeared later when mice became familiar with the environment (3rd – 8th session) (Figure 2—figure supplement 2B). In the novel object recognition test, female Ox1R^ΔDAT mice spent more time with each of the two identical objects in an open field compared to control mice (Figure 2—figure supplement 2E). One hour later, when female mice were exposed to the same environment except that one object was replaced by a novel object, there was no significant difference between control and Ox1R^ΔDAT mice in exploring the novel object (Figure 2—figure supplement 2F). In contrast, male Ox1R^ΔDAT mice did not behave differently in the hole-board and novel object recognition tests from controls (Figure 2—figure supplement 2A, C, D). This indicates that Ox1R signaling in dopaminergic neurons is more important for females in novelty-induced exploration, which was independent of novel object discrimination.

The behavioral changes seemed not related to alterations in anxiety since there were no detectable changes in anxiety-related behaviors, including the time spent in and the times of entries into the center area, light side and the open arms in an open field test, dark/light box test and 0-maze test, respectively (Figure 2—figure supplement 3).

Ox1R deletion in dopaminergic neurons did not change energy metabolism and reward processing

The orexin and dopaminergic systems are well known to regulate energy metabolism and reward processing (Howe & Dombeck, 2016; Inutsuka & Yamanaka, 2013; Narayanan et al., 2010; Palmiter, 2007; Tsujino & Sakurai, 2013). Therefore, we measured the impact of Ox1R deletion in dopaminergic neurons on metabolism and reward-related behaviors.

In the two-bottle preference test, mice have free access to both water and sweet solution (sucrose solution or the non-caloric sweetener, sucralose solution). In both of the control and Ox1R^ΔDAT groups, the percentages of sweet solution consumption in the total drinking were higher than 50%, indicating the preference of sweet solution (Figure 2—figure supplement 4A, D). The sucrose preference was more obvious for 1% and 2% than 0.5% sucrose solution (Figure 2—figure supplement 4B, E). There was no significant difference between the two groups (Figure 2—figure supplement 4A, B, D, E).

For the conditional place preference test (CPP, cocaine- vs. saline-injection-paired compartment), CPP score was calculated to show how much cocaine injection changed the time that mice spent in the compartment. Both of the control and Ox1R^ΔDAT groups showed a preference for cocaine injection without a significant difference between the two groups (Figure 2—figure supplement 4C, F).

In addition, neither male nor female Ox1R^ΔDAT mice showed significant changes in energy metabolism, including body weight, fat mass, respiratory exchanging ratio, energy expenditure, daily food intake, glucose tolerance and insulin sensitivity (Figure 2—figure supplement 5).

Ox1R in dopaminergic neurons regulates specific neural pathways under different conditions

To explore and map the underlying neuronal pathways of the orexin-mediated behaviors unbiasedly, we performed positron emission tomography (PET) imaging of control and Ox1R^ΔDAT mice upon ICV injection of orexin A or saline (control).

In Ox1R^ΔDAT mice under control conditions (saline injection), we observed higher neuronal activity around the medial preoptic area (MPA), piriform cortex (Pir), endopiriform claustrum, lateral stripe of the striatum (LSS), ventral part of subcoeruleus nucleus (SubCV), spinal trigeminal nucleus, spinal trigeminal tract (sp5), the intermediate reticular zone (IRt) and the gigantocellular reticular nucleus (Gi) than control mice (Figure 3A, Figure 3—figure supplement 1A and 2). Compared to control mice, central application of orexin A (1 nmol, ICV) to Ox1R^ΔDAT mice induced higher neuronal activity around the lateral paragigantocellular nucleus (LPGi), nucleus of the horizontal limb of the diagonal band (HDB) and magnocellular preoptic nucleus (MCPO), and lower activity around secondary motor cortex (M2), the dorsal bed nucleus of the stria terminalis (BNST), the hindlimb, shoulder, and trunk regions and barrel field of primary somatosensory cortex (S1HL, S1Sh, S1Tr, and S1BF), the lateral area of secondary visual cortex (V2L), primary visual cortex (V1), Pir and dorsal endopiriform claustrum (DEn) (Figure 3B, Figure 3—figure supplement 1B and 2). PET imaging did not reveal significant changes in the VTA and SN (Figure 3, Figure 3—figure supplement 1).

Figure 3

Postmortem analysis of c-Fos staining revealed low c-Fos expression in dopaminergic neurons in the VTA and SN of Ox1R^ΔDAT and control mice after ICV injection of saline or orexin A (1 nmol). No apparent changes were observed among the groups. In contrast, clear orexin-induced c-Fos activity was observed in non-dopaminergic cells (Figure 3—figure supplement 3). This could affect the interpretation of dopaminergic contribution to behavioral effects in studies using ICV or intra-VTA/SN injection of orexin. Further studies would be needed to investigate how orexin directly and indirectly affect the VTA and SN neurons.

Dopamine receptors in the dorsal BNST and LPGi

Based on the PET imaging and behavioral findings, we further focused on the dorsal BNST and LPGi. They play a key functional role in regulating emotion, exploratory behaviors and locomotor speed, which are related to novelty-induced locomotion (Arber & Costa, 2022; Avery et al., 2016; Capelli et al., 2017; Crestani et al., 2013; Giardino et al., 2018; Takatoh et al., 2013). A schematic illustration of these analyzed brain areas is shown in Figure 4—figure supplement 1. We analyzed the dopaminergic pathways into the dorsal BNST and LPGi that could have been affected by the Ox1R. To this end, we injected Cre-dependent AAV-EYFP bilaterally into the VTA and SN of DAT-Cre (DAT^EYFP) and Ox1R^ΔDAT (DAT^{ΔOx1R; EYFP}) mice, and discovered dopaminergic projections in the dorsal BNST and LPGi (Figure 4—figure supplement 2), suggesting that VTA and SN dopaminergic neurons could directly modulate neuronal activity in the dorsal BNST and LPGi. To further explore this idea, we assessed the expression of Fos and dopamine receptors in the dorsal BNST and LPGi by immunostaining and RNAscope experiments.

Orexin A injection (1 nmol, ICV) induced higher c-Fos expression levels in LPGi of male Ox1R^ΔDAT mice compared to control mice (Figure 4A, C). Only the inhibitory D2 but not excitatory D1 subtypes of dopamine receptors (DRD2, DRD1) could be detected in LPGi (Figure 4D, E). Both receptors were detected around the lateral ventricle (as positive control), whereas no signal was detectable in LPGi incubated with a negative control probe for mice (Figure 4F, G). Th-positive fibers were detected in LPGi but not Gi (Figure 4A, B), consistent with previous reports (Kitahama et al., 2000). These data provides the anatomical basis for the possibility that Ox1R deletion in dopaminergic neurons results in the disinhibition of neural activity in LPGi via dopaminergic pathways.

Figure 4

Orexin A injection increased c-Fos expression in the dorsal BNST in control but not in Ox1R^ΔDAT mice (Figure 4H, J). The expression of DRD1 was significantly reduced, and DRD2 expression levels showed a tendency towards a decrease in dorsal BNST of Ox1R^ΔDAT mice (Figure 4K-M, Figure 4—figure supplement 3). The Th expression levels were not changed between genotypes (Figure 4H, I, Figure 4—figure supplement 3). This indicates that the dorsal BNST could be less activated due to the decrease of DRD1 and the lack of orexin-mediated activation of dopaminergic neurons.

Discussion

This study’s behavioral experiments provide important evidence that Ox1R deficiency in DAT-expressing neurons leads to an increase in novelty-induced locomotion and exploration without altering energy metabolism and anxiety-or reward-processing-related behaviors. We found that Ox1R is the main subtype of orexin receptors in midbrain DAT-expressing neurons. The essential prerequisites for these functional studies were RNAscope and ex vivo Ca²⁺ imaging experiments, which confirmed the successful knockout of Ox1R in dopaminergic neurons. Further PET imaging, along with c-Fos and DRD expression analyses, provided initial insights into the underlying mechanisms. These findings can serve as a basis for developing testable working hypotheses on the systemic physiological role of orexin signaling.

Earlier studies showed orexin-induced activation of VTA dopaminergic neurons (Baimel et al., 2017; Korotkova et al., 2003; Tung et al., 2016). Whole-cell patch clamp recordings combined with mRNA measurement of the individually recorded VTA neurons revealed fewer Ox2R-than Ox1R-positive dopaminergic neurons (Korotkova et al., 2003), which is in line with our findings. While our electrophysiological and Ca²⁺ imaging data clearly show an orexin A-induced activation of dopaminergic SN neurons when GABAergic and glutamatergic synaptic input is blocked, previous in vitro (Korotkova et al., 2002) or in vivo extracellular electrophysiological recordings in rats (Liu et al., 2018) studies were controversial about whether orexin A could activate dopaminergic neurons in the SN. Since Ox1R and Ox2R are expressed by non-dopaminergic neurons around the SN, recordings in the absence of synaptic blockers, could detect a mixed direct and indirect orexin-induced response in dopaminergic neurons (Nair-Roberts et al., 2008). This may partially explain their controversial findings.

We found that Ox1R deficiency in dopaminergic neurons caused an increase in novelty-induced locomotion and exploration, which could indicate elevated arousal levels and hyperactivity. Interestingly, children with attention deficit and hyperactivity disorder (ADHD) have lower orexin A but unaltered orexin B levels in plasma compared to the healthy controls (Baykal et al., 2019). ADHD patients exhibit impaired dopaminergic functions, and medicine improving dopaminergic signaling is used to treat ADHD in clinics (Feldman & Reiff, 2014; Volkow & Swanson, 2013). Ox1R signaling in dopaminergic neurons is important to limit novelty-induced arousal and hyperactivity, which are related to task performance and learning capability. Novelty-induced behavioral response should be at proper levels under normal physiological conditions. The orexin-dopamine interaction blunting novelty-induced locomotion could be important to keep attention on the main task without being distracted too much by other random environmental stimuli. When this balance is disrupted, behavioral deficit may happen, such as ADHD.

We did not observe significant impact of Ox1R signaling in dopaminergic neurons on reward processing. Previous studies reported that Ox1R signaling in VTA promoted reward processing with pharmacological methods (Tsujino & Sakurai, 2013). The difference could have resulted from the involvement of non-dopaminergic neurons in VTA or different behavioral models. It would be interesting to identify the effect under other conditions, such as obesity and operant reward learning, in the future (Berke, 2018; Dabney et al., 2020; Kim et al., 2020; Wang et al., 2001).

The PET imaging results suggest that Ox1R signaling in dopaminergic neurons affects separate and distinct neuronal circuits, depending on the stimulation mode. Orexin and dopaminergic systems are known to increase arousal in mammals’ sleep-wake transitions, locomotor activity, and motivation (Berke, 2018; Berridge et al., 2010; Furlong et al., 2009; Mahler et al., 2014). The orexin system responds to various environmental stimuli, such as stress and novelty (Berridge et al., 2010; Gonzalez et al., 2016). Projection-defined dopaminergic populations in the VTA exhibited different responses to orexin A (Baimel et al., 2017). Our PET imaging experiments, without orexin application, indicate that Ox1R signaling in dopaminergic neurons modulates brain regions (MPA, Pir, endopiriform claustrum, LSS, SubCV, spinal trigeminal nucleus, sp5, IRt and Gi) important for olfaction, eating, arousal and sleeping (Supplementary table 1).When central orexin levels were increased by orexin A injection, Ox1R signaling in dopaminergic neurons regulates HDB, MCPO, Pir, DEn, S1, V2L, V1, BNST, LPGi and M2, which are important for sensation, emotion, exploration, and action selection (Supplementary table 1). In future studies, it would be interesting to investigate the direct and indirect downstream pathways of Ox1R-expressing dopaminergic neurons. Interestingly, in the fruitfly Drosophila melanogaster, it has been shown that the dopaminergic system oppositely regulates sleep-wake arousal and environmentally stimulated arousal, and the authors discussed the possibility to identify similar patterns in mammals (Lebestky et al., 2009). It was further suggested that the dopaminergic system differentially regulates arousal levels under different stimulating conditions (Lebestky et al., 2009; Lima & Miesenbock, 2005). Emotion perception affects the decision how to respond to the novelty. We think that novelty activates the orexin system, and Ox1R signaling in dopaminergic neurons promotes emotion perception and inhibits exploration.

DRD1 and DRD2 in the BNST regulate anxiety, fear, and addiction (De Bundel et al., 2016; Eiler et al., 2003; Giardino et al., 2018; Krawczyk et al., 2013). LGPi regulates exploratory behaviors and locomotor speed (Takatoh et al., 2013; Arber & Costa, 2022; Capelli et al., 2017). We found a potential direct impact of dopaminergic neurons on LPGi and BNST. The inhibitory D2 receptor was mainly expressed in LPGi, and the expression levels of the excitatory D1 receptor were decreased in BNST after Ox1R deletion in dopaminergic neurons. Our findings suggested that Ox1R deletion in dopaminergic neurons results in the disinhibition of neural activity in LPGi via dopaminergic pathways and the decrease of dopamine-mediated neural activity in BNST. It would be very interesting to further investigate these pathways in future. There are few publications on the functional relevance of dopaminergic projections in the LPGi. Future studies would be very interesting in terms of functionally analyzing DRD2 signaling in the LPGi and how orexin interacts with DRD2 signaling.

Materials and Methods

Animal care

All animal procedures were conducted in compliance with protocols approved by the local government authorities (Bezirksregierung Cologne, Germany) and were in accordance with National Institutes of Health guidelines. Mice were housed in groups of 3 -5 at 22°C - 24°C using a 12-hr light/12-hr dark cycle. Animals had ad libitum access to water and food at all times. Animals were fed a NCD (V1554, ssniff^R), which contains 67% calories from carbohydrates, 23% calories from protein, and 10% calories from fat.

Generation of Ox1R^ΔDAT mice

Mice carrying a loxP-flanked Ox1R-allele (Ox1R fl/fl) were generated in our facility (Xiao et al., 2021). Ox1R fl/fl mice were crossed with Dat-Cre mice (Ekstrand et al., 2007) to obtain Ox1R fl/fl, Dat-Cre tg/wt mice, i.e. dopaminergic-specific Ox1R knockout mice on a C57/Bl6 N background (Ox1R^ΔDAT). Mice from the same breeding without the Cre transgene were used as controls (Ox1R fl/fl, Dat-Cre wt/wt).

Glucose and insulin tolerance tests

Glucose tolerance tests were performed on 12-weeks-old animals that had been fasted for 6 hours from 9 am. Insulin tolerance tests were performed on 11-weeks-old random-fed mice around 9 am. Animals received an intraperitoneal injection of 20% glucose (10 ml/kg body weight; KabiPac) or insulin (0.75 U/kg body weight; Sanofi-Aventis) into the peritoneal cavity, respectively. Blood glucose values were determined in whole venous blood using an automatic glucose monitor (Contour^R, Bayer). Glucose levels were determined in blood collected from the tail tip immediately before and 15, 30, and 60 minutes after the injection, with an additional value determined after 120 minutes for the glucose tolerance tests.

Analysis of body composition

Nuclear magnetic resonance (NMR) was employed to determine whole body composition of live animals on NCD at the age of 20 weeks using the NMR Analyzer minispec mq7.5 (Bruker Optik, Ettlingen, Germany).

Histology

Brains from male mice were removed, post-fixed in 4% paraformaldehyde (PFA) for the indicated time, and dehydrated in 20% sucrose in 0.1M phosphate-buffered saline (PBS) overnight. Brains were stored at -80°C until cutting.

Immunostaining

Brains were post-fixed for 6 h at 4 °C and cut (30 µm) with Leica CM3050 S Research Cryostat. Sections were incubated in 0.3% glycine for 10 min after washing in 0.1 M PBS for 2 × 10 min. After washing in PBS for another 10 min, sections were incubated in 0.03% SDS (in PBS) for 10 min before they got blocked with 3% donkey serum (in PBS, 0.25% Triton X-100) for 1 h at RT. Afterwards, they were incubated with primary antibodies, including goat anti-c-Fos (sc-526, Santa Cruz Biotechnology), chicken anti-GFP (ab13970, Abcam) and/or rabbit anti-Th (ab112, Abcam), for overnight (GFP) or 48 h (c-Fos and Th) at 4°C, washed in PBS for 3 × 10 min, and incubated with secondary antibodies, including Alexa Fluro 594 donkey anti-goat, FITC donkey anti-chicken, Alexa Fluro 488 donkey anti-rabbit (a11058, sa 1-7200 and a21206, Invitrogen), for 1 h at RT. After washing in PBS for 3 × 10 min, slices were mounted and covered with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories). Slices were stained together at one time for each experiment to have identical conditions for comparable signals. Slides were stored at 4 °C until imaging. Images were obtained with confocal laser scanning microscope Leica TCS SP8.

RNAscope fluorescent in situ hybridization

Brains from 12-wk-old male mice were post-fixed for 20 - 22 h at RT and cut (20 µm). Slides were stored at -80°C until staining. All reagents were purchased from Advanced Cell Diagnostics, and the staining was performed with the RNAscope Multiplex Fluorescent v2 kit (ACD, Advanced Cell Diagnostics) according to the user manual. Probes for Ox1R (Hcrtr1, 471561-C3, ACD) and Ox2R (Hcrtr2, 471551, ACD), DRD1 (Drd1a, 406491-C2, ACD), DRD2 (Drd2, 406501), and Th (Th, 317621-C4) were purchased from ACD. In brief, slides were briefly washed in diethyl-pyrocarbonate (DEPC)-treated Millipore water, air dried, and then dried at 60°C overnight. On the second day, slides were treated with hydrogen peroxide (H₂O₂) for 10 min at RT, washed in water, and boiled in Target Retrieval solution (around 99.4 °C) for 8 -10 min. After a brief washing in water and dehydration in absolute ethanol, slides were incubated with protease IV for 30 min at RT. Slides were washed again in water and hybridized with the mixture of probes in different channels for 2 h in a humidified chamber at 40 °C. Afterwards, the hybridization was amplified with AMP 1 for 30 min, AMP 2 for 30 min and AMP 3 for 15 min. The signal was then developed for each channel. For example, for channel 1, slides were incubated with HRP-C1 for 15 min, the fluorophore for 30 min, and HRP blocker for 15 min. All amplification and development were performed at 40 °C, and 2 × 2 min of washing in ACD wash buffer was performed after each step. Finally, the slides were counterstained with DAPI for 1 min, mounted with Prolong^TM Gold Antifade reagent with DAPI (Invitrogen), and covered with coverslips.

Slides were dried and stored at 4 °C. Imaging was performed with a confocal laser scanning microscope Leica TCS SP8.

Quantification

The images were manually analyzed with Image J/FIJI (version 1.50d). Dopaminergic cells were manually identified and outlined based on Th and DAPI signal. The integrated intensity of Ox1R and Ox2R signal in the delineated cells was automatically calculated by Image J. The integrated intensity in the negative control area was used as threshold, so that the cells with integrated intensity higher than the threshold were identified as Ox1R-or Ox2R-positive dopaminergic cells. c-Fos positive cell numbers were manually counted. Th, DRD1 and DRD2 fluorescence in the respective areas was taken as the integrated intensity automatically calculated by Image J.

Ca²⁺ imaging and electrophysiology

Animals and brain slice preparation

Perforated patch clamp recordings and Ca²⁺ imaging of dopaminergic SN neurons in acute brain slices were carried out essentially as described in Hess et al. (2023) and Xiao et al. (2021). Ca²⁺ imaging experiments were performed on brain slices from 18-19 week-old male control and Ox1R^ΔDAT mice. Animals were kept under standard laboratory conditions, with tap water and chow available ad libitum, on a 12h light/dark cycle. The animals were lightly anesthetized with isoflurane (B506; AbbVie Deutschland GmbH and Co KG) and decapitated. Coronal slices (280 µm) containing the SN were cut with a vibration microtome (VT1200 S; Leica) under cold (4°C), carbogenated (95% O₂ and 5% CO₂), glycerol-based modified artificial cerebrospinal fluid (GaCSF). GaCSF contained (in mM): 244 Glycerol, 2.5 KCl, 2 MgCl₂, 2 CaCl₂, 1.2 NaH₂PO₄, 10 HEPES, 21 NaHCO₃, and 5 Glucose adjusted to pH 7.2 with NaOH. Brain slices were transferred into carbogenated artificial cerebrospinal fluid (aCSF). First, they were kept for 20 min in a 35°C ’recovery bath’ and then stored at room temperature (24°C) for at least 30 min before recording. During the recordings, the brain slices were continuously superfused with carbogenated aCSF at a flow rate of ∼2.5 ml·min^-1. aCSF contained (in mM): 125 NaCl, 2.5 KCl, 2 MgCl₂, 2 CaCl₂, 1.2 NaH₂PO₄, 21 NaHCO₃, 10 HEPES, and 5 Glucose adjusted to pH 7.2 with NaOH. To block GABAergic and glutamatergic synaptic input, the aCSF contained 10^-4 M PTX (picrotoxin, P1675; Sigma-Aldrich), 5 x 10^-6 M CGP (CGP-54626 hydrochloride, BN0597, Biotrend), 5 x 10^-5 M DL-AP5 (DL-2-amino-5-phosphonopentanoic acid, BN0086, Biotrend), and 10^-5 M CNQX (6-cyano-7-nitroquinoxaline-2,3-dione, C127; Sigma-Aldrich). Orexin A (ab120212, Abcam) was bath-applied via the superfusion system at concentrations of 100 nM and 300 nM for 8 min for each concentration.

Perforated patch clamp recordings

Perforated patch clamp recordings were performed in current clamp mode. The experiments were carried out using protocols modified from previous studies, as summarized in Hess et al. (2023). SN dopaminergic neurons were identified according to their sag component / slow I_h-current (hyperpolarization-activated cyclic nucleotide-gated cation current), broad action potentials (Lacey et al., 1989; Neuhoff et al., 2002; Richards et al., 1997) and post hoc by biocytin-streptavidin labeling combined with TH-immunohistochemistry (Hess et al., 2013). The electrode solution contained (in mM): 140 K-gluconate, 10 KCl, 10 HEPES, 0.1 EGTA, 2 MgCl₂, and 1% biocytin (B4261, Sigma) adjusted to pH 7.2 with KOH. The calculated liquid junction potential between intracellular and extracellular solution was compensated. The patch electrode was tip-filled with electrode solution and backfilled with electrode solution, which contained the ionophore amphotericin B (A4888; Sigma) to achieve perforated patch recordings, 0.02% tetramethylrhodamine-dextran (3,000 MW, D3308, Invitrogen) to monitor the stability of the perforated membrane, and 1 % biocytin (B4261; Sigma-Aldrich) to label the recorded neuron. Amphotericin B was dissolved in dimethyl sulfoxide (DMSO; D8418, Sigma) to a concentration of 200 µg*µl^-1 and added to the electrode solution. The ionophore was added to the modified electrode solution shortly before use. The final concentration of amphotericin B was ∼120-160 µg*ml^-1.

The orexin A (ab120212, Abcam) effect was analyzed by comparing the action potential frequencies measured during 4 min intervals recorded before and at the end of the peptide applications. To analyze the orexin A responsiveness, the neuron’s firing rate averaged from 10 s intervals was taken as one data point. To determine the mean firing rate and standard deviation, 24 data points were averaged. On the single-cell level, a neuron was considered orexin-responsive if the change in firing induced by orexin A was three times larger than the standard deviation (3 σ criterion ≙ Z-score of 3). The significance of the mean response (Δfrequency) was tested using a one-sample Wilcoxon signed rank test. Data analysis was performed using Spike2 (version 7; Cambridge Electronic Design Ltd.), Igor Pro 6 (Wavemetrics), and Prism 8 (GraphPad Software Inc.).

Ca²⁺ imaging

All imaging experiments were performed on acute brain slices using the genetically encoded calcium indicator GCaMP6. At least 4 weeks were allowed for GCaMP6 virus expression. The imaging setup consisted of a Zeiss AxioCam/MRm CCD camera with a 1388x1040 chip and a Polychromator V (Till Photonics) that was coupled via an optical fiber into the Zeiss AxioExaminer upright microscope (Objective W "Plan-Apochromat" 20x/1.0 DIC D=0.17 M27 75mm). The camera and polychromator were controlled by the software Zen pro, including the module ‘Physiology’ (2012 blue edition, Zeiss). The SN neurons were identified according to their anatomical location and expression of the GCaMP6. Calcium signals in GCaMP6 expressing cells were monitored by images acquired at 470 nm excitation wavelengths with 20 ms exposure time at ∼0.2 Hz. The emitted fluorescence was detected through a 500-550 nm bandpass filter (BP525/50), and data were acquired using 4x4 on-chip binning. Images were recorded in arbitrary units (AU) and analyzed as 16-bit grayscale images. Orexin A (ab120212, Abcam) in the concentrations of 100 nM and 300 nM was applied for 8 min each. To analyze the orexin effect, we compared the fluorescence measured in the cell bodies during 4 min intervals that were recorded immediately before and at the end of the peptide applications. This protocol was followed by applying high K⁺ (40 mM) concentration saline (40 mM KCl; osmolarity adjusted by reducing the NaCl concentration) to identify cell bodies of neurons with functional GCaMP6 expression. Regions of interest (ROI) were defined after the experiment based on the high K⁺ saline responses. The mean AU values of the ROIs were calculated in Image J. Time series analysis was performed with Igor Pro 6. To correct for bleaching artifacts, the baseline fluorescence (without orexin application) was fit. The extended fit was subtracted from the raw data. For each neuron, we tested whether it responded to orexin A. A neuron was considered orexin-responsive if the change in GCaMP fluorescence induced by orexin A was three times larger than the standard deviation (3 σ criterion ≙ Z-score of 3) of the baseline fluorescence. To determine the "population response" of all analyzed neurons, we measured the orexin-induced GCaMP6 fluorescence of each neuron expressed as a percentage of the GCaMP6 fluorescence induced by the high (40 mM) K⁺ saline application. This is a solid reference point since it reflects the GCaMP6 fluorescence at maximal voltage-activated Ca²⁺ influx. The "population response" of all analyzed neurons was expressed as the mean ± SEM of these responses. The significance of this mean response was tested for each group (control and Ox1R^ΔDAT) using a one-sample t-test. Image analysis was performed offline using Image J (version 1.53a), Igor Pro 6 (WaveMetrics), and Prism 8 (GraphPad Software Inc.).

Food intake and spontaneous activity

Mice (20 weeks old) were analyzed for food intake, spontaneous activity, respiratory exchanging ratio and energy expenditure in a PhenoMaster System (TSE Systems) as previously described (Jordan et al., 2011).

Behavioral studies

Mice were moved into the behavioral test room, weighed, and handled 1 week before the measurement. On the day of the tests, mice (9-15 weeks old) were moved near to the equipment and handled 1 h before the tests. There was a 1-week break between different behavioral tests for anxiety, exploration or locomotion when using the same mice.

Open field test (OFT), dark/light (D/L) box and Hole-board tests

OFT and D/L box tests were performed with a Seamless Open Field Starter Package for Mouse plus the Light/Dark Insert or the hole-board floor (Med Associates, Inc.) between 10 am to 4 pm.

To start OFT, mice were put in the middle of a clear Plexiglas^® chamber (27.3 cm ξ 27.3 cm ξ 20.3 cm) sitting in a sound-attenuating cubicle, and mouse movement was automatically traced for 30 min with 48 infrared beams divided into the X, Y and Z plane. For the D/L box test, a black Plexiglas^® box (half the size of the chamber) was inserted at the left side of the chamber, with an opening at the middle side. Mice were put in the middle of the light side and faced the openings. Mouse movement was traced for 5 min. For the hole-board test, a metal hole-board floor with 16 equally spaced holes for exploration was inserted in the chamber. Mice were put in the middle of the chambers and the nose pokes were monitored automatically for 10 min per session. There was 1 h break between each session. 4 sessions per day were analyzed for 2 consecutive days.

Elevated 0-maze test

An elevated 0-maze test was performed with TSE elevated 0-Maze in combination with the video tracking system TSE VideoMot 3D version 7.01. There are 2 closed and 2 open runways without a center position. The outer diameter is 48 cm, the arm width is 5 cm, the wall height is 10 cm and the base height is 55 cm. Mice were put in the middle of an open arm and faced to the closed arm to start the experiment. Mouse movement was monitored for 5 min.

Novel object recognition test

Novel object recognition test was modified from the publication before (Ruud et al., 2019). The mouse was acclimated and measured for 10 min in the 1st session and for 5 min in the 2nd session on the same day, in a polycarbonate box with non-transparent walls (50 cm ξ 50 cm ξ 30 cm). Two objects were present in the box. In the 1st session, two identical objects (glass beaker filled with blue marbles) were present and fixed with odor-free adhesive to the ground flooring at two sides of the box delineated diagonally. In each diagonal corner, the object is 11.5 cm and 14 cm from each wall respectively, and the distance between the two objects is 17.5 cm. Mice always started at the same corner which was between the two fixed objects and were allowed to explore and learn the experimental area and objects. In the 2nd session which started after a 1-h break in the home cage, one of the two identical objects was replaced with a novel object (Lego^® Primo brick), and mice were allowed again to explore the environment and objects. The positions of the mouse nose, tail and center were monitored with a video tracking system TSE VideoMot 2. The interaction with a fixed object was identified when the nose was facing the object within a distance of 2 cm.

Conditioned place preference test for cocaine

The test was modified from previous publication (Ruud et al., 2019), and was performed in a polycarbonate box with non-transparent walls. The box was separated into 3 compartments with 2 independent partitions. It contains a left compartment with zebra stripes (white and black) painted on the wall and a rough ground (16.5 x 13.2 x 21.2 cm), a small middle area (7.0 x 13.2 x 21.2 cm), and a right compartment with a white wall and smooth ground. Mouse movement was monitored with TSE VideoMot 2 for 30 min per session, 1 session per day. Mice started from the middle area and had access to both left and right compartments through an opening (4.0 cm x 4.2 cm) in the partition during the habituation and pre-test days (d1-3) and on the test day (d10), and were put into and kept in either the left or right compartment without an opening in the partition during the conditioning phase (d4-9). The preference of the two compartments by nature was analyzed during the pre-test days. At the conditioning phase, on alternative days, mice received i.p. injection of cocaine (5 mg/kg BW) or saline immediately before entering the respective compartments. Cocaine and saline were always paired to the non-preferred and preferred compartments, which were determined by pre-tests, respectively. There was no injection on the habituation, pre-test or test days. The percentage of time spent in the reward-paired compartment was calculated: 100 x time spent in the compartment / (total time -time spent in the middle area). The CPP score was then analyzed using the calculated percentage of time: 100 x (time on the test day – time on pre-test days)/ time on pre-test days. The pre-test and test days were before and after the conditioning, respectively. Thus, the CPP score above zero indicates that the CPP preference is developed.

Sucrose/sucralose preference test

Two-bottle tests were performed with 2 15-ml falcon tubes connected to sippers. Mice were single caged 1 week before the beginning of the experiment. 2 falcon tubes filled with water (side-by-side) replaced the normal water bottle. Water intake was monitored for a week to make sure mice adjusted to drinking from the sippers. When the test started, 2 new sipper tubes of water were used for 2 days of baseline measurement and were then replaced with one containing water and one containing sucrose/sucralose solution for 2 days of preference measurement. Animals received a 0.5%, 1% and 2% (w/v) sucrose solution and 1 mM sucralose solution in sequential. Between the preference tests of different solutions, 2 days of baseline measurement were performed with 2 new sipper tubes of water as a washout. To avoid side bias, the positions of 2 sipper tubes were switched every day, with the sucrose/sucralose tube always starting on the non-preferred position, which was determined by water intake on the day before the preference measurement. Sipper tubes were weighed every day.

ICV cannula implantation and injection

12-wk-old mice received 1 mg/ml of tramadol in drinking water 2 d before the surgeries. On the day of surgery, mice were anesthetized with isoflurane, and a bolus of buprenorphine (0.1 mg/kg BW) was given (i. p.) to reduce pain. Brain regions were identified according to the atlas (Franklin & Paxinos, 2007). After alignment of the brain in the stereotaxic surgery platform, one small hole was drilled in the skull and the ICV cannula (26 gauge, Plastics One) was immediately implanted (AP: -0.2 mm, ML: +1 mm, DV: -2.4 mm) and fixed to the skull with dental acrylic (Super-Bond C&B). The dummy cannula caps were inserted to close and protect the cannula. Mice received a bolus of meloxicam (5 mg/kg BW, s.c.), and tramadol in drinking water for 3 d after surgeries to relieve pain. BW was checked twice a day. Experiments started at least 1 week later. For injection, the catheter was attached to an injector (Plastics One), which was inserted through the cannula. orexin A (Phoenix Pharmaceuticals) was injected slowly at the dose of 1 nmol dissolved in 2 ul of saline. Starting from 5 days before the injection, mice and the dummy cannula caps were handled for acclimation daily. The locomotion activity was measured in a PhenoMaster System (TSE Systems), and mice were acclimated to the system at least 5 days before the test.

PET imaging

PET imaging was performed using an Inveon preclinical PET/CT system (Siemens). Male mice were anesthetized with 2% isoflurane in 65%/35% nitrous oxide/oxygen gas, injected with saline or orexin A (Phoenix Pharmaceuticals) and positioned on a dedicated mouse carrier (MEDRES, Germany) carrying two mice. Body temperature was maintained at 37.0 ± 0.5°C by a thermostatically controlled water heating system. For injection of the radiotracer, a catheter consisting of a 30G cannula connected to a polythene tubing (ID = 0.28 mm) was inserted into the tail vein and fixated by a drop of glue. After starting the PET scan, 7-8 MBq of [18F]FDG in 50-100 µl saline were injected per mouse. Emission data were acquired for 45 minutes. Thereafter, animals were automatically moved into the CT gantry, and a CT scan was performed (180 projections/360°, 200 ms, 80 kV, 500 µA). The CT data were used for attenuation correction of the PET data, and the CT image of the scull was used for image co-registration. Plasma glucose levels were determined from a tail vein blood sample using a standard glucometer (Bayer) after removing the tail vein catheters. PET data were histogrammed in time frames of 12x30s, 3x60s, 3x120s, 7x240s, Fourier rebinned, and images were reconstructed using the MAP-SP algorithm provided by the manufacturer. For co-registration, the imaging analysis software Vinci was used (Cizek et al., 2004). Images were co-registered to a 3D mouse brain atlas constructed from the 2D mouse brain atlas published by Paxinos (Paxinos et al., 2013).

Kinetic modeling

An image-derived input function was extracted from the PET data of the aorta, which could be identified in the image of the first time frame of each animal. Input function data were corrected for partial volume effect by assuming a standardized volume fraction of 0.6 (Green et al., 1998). Parametric images of the [18F]FDG kinetic constants K₁, k₂, k₃, and k₄ were determined by a voxel-by-voxel (voxel size = 0.4 mm x 0.4 mm x 0.8 mm) fitting of data to a two-tissue-compartment kinetic model. K₁ is the constant for transport from blood to tissue, k₂ for transport from tissue to blood, k₃ the constant for phosphorylation of [18F]FDG to [18F]FDG-6-phosphate, and k₄ the constant for dephosphorylation. The ratio of tissue and plasma glucose concentrations (C_E/C_P) is a measure for glucose transport and is given by C_E/C_P = K₁/(k₂ + k₃/0.26) (Backes et al., 2011; Jais et al., 2016). Since neuronal activation is accompanied by increased glucose transport and this parameter is less sensitive to changes in plasma glucose level, we use alterations of glucose transport (C_E/C_P) as surrogate for alterations in neuronal activation.

Statistics

Statistical testing was performed by application of a voxel-wise t-test between groups. 3D maps of p-values allow for the identification of regions with significant differences in the parameters. From these regions, we defined volumes of interest (VOIs) and performed additional statistical testing for these VOIs. For presentation only, 3D maps of p-values were re-calculated on a 0.1 mm x 0.1 mm x 0.1 mm grid from the original dataset using trilinear interpolation.

Virus injection

Male mice received 1 mg/ml of tramadol in drinking water 2 d before the surgeries. On the day of surgery, mice were anesthetized with isoflurane and a bolus of buprenorphine (0.1 mg/kg BW) was given (i. p.) to reduce pain. Brain regions were identified according to the atlas (Franklin & Paxinos, 2007). After alignment of the brain in the stereotaxic surgery platform, 1 or 4 small holes were drilled in the skull at specific coordinates. The virus, pAAV-Ef1a-DIO EYFP (∼1x10¹³ GC/ml, 300 nl, Addgene) was bilaterally injected into VTA and SN of 10 wk old mice, with micropipettes pulled in house with a heating system. The coordinates from Bregma were: anterior-posterior, AP: -3.4 mm; medial-lateral, ML: ±0.5/1.25 mm; dorsal-ventral, DV: -4.2 mm. The GCaMP6 virus (AAV1.Syn.Flex.GCaMP6s.WPRE.SV40, ∼4x10¹² GC/ml, 300 nl, Penn Vector Core) was injected to left SN (AP: -3.4 mm, ML: 1.1 mm, DV: -4.2 mm) of 12 wk old mice, to specifically express GCaMP6 in dopaminergic neurons. After virus injection, mice received a bolus of meloxicam (5 mg/kg BW, s.c.), and tramadol in drinking water for 3 d after surgeries to relieve pain. BW was checked twice a day. Experiments started at least 4 weeks later than virus injection to allow virus expression.

Statistical methods

All numerical values are expressed as the mean ± SEM. Statistical analyses were conducted using GraphPad PRISM (version 8) unless stated otherwise. Datasets with only two independent groups were analyzed for statistical significance using an unpaired two-tailed Student’s t-test. Datasets subjected to two independent factors were analyzed using two-way ANOVA followed by Sidak’s post hoc test. All p-values < 0.05 were considered significant (*p < 0.05, **p < 0.01, and ***p < 0.001, ****p < 0.0001).

Acknowledgements

We acknowledge Hella Brönneke for outstanding support, Jens Alber, Pia Scholl, Christiane Schäfer, Nadine Spenrath, Ina Stünkel, Kerstin Marohl, Antonia Lorenz and Helmut Wratil for outstanding technical assistance. We received funding by the DFG within the framework of the TRR 134 (A.C.H., P.K.), DFG-401832153 (P.K.) and within the Excellence Initiative by German Federal and State Governments (CECAD). This work was funded (in part) by the Helmholtz Alliance ICEMED (Imaging and Curing Environmental Metabolic Diseases) through the Initiative and Networking Fund of the Helmholtz Association. X.X. received funding from CECAD Family Support. G.Y. gratefully acknowledges financial doctoral support from the DFG-233886668/GRK1960.

Author Contributions

X.X. and A.C.H. conceived the project, designed the experiments, and wrote the manuscript with input from the other authors. A.C.H. generated the Ox1R flox mouse line. X.X. performed all the experiments apart from calcium imaging and electrophysiological recording experiments (G.Y., F.E. and P.K.) and PET imaging experiments (A.L.C. and H.B.). All authors discussed the data, commented on the manuscript before submission, and agreed with the final submitted manuscript.

Competing Interests

The authors declare no competing interests.

Data Availability

There are no restrictions on data availability.