a) and b) Schematic of nanopipette delivered triggering of TLR4 and Myddosome formation with LPS or Aβ aggregates. The LPS is delivered by nanopipette to the macrophage surface. When LPS binds to TLR4, it will trigger the dimerization of TLR4, which activates the recruitment of Mal, MyD88 and IRAK4&2 to form the Myddosome. Unstimulated TLR4 remains monomeric on the cell surface. c) Schematic of AFM cantilever light sheet 3D scan. The light sheet is reflected by an AFM cantilever to the target cell. Control by a piezo, the sample stage is moving up and down to achieve cell scanning.