Phylogenetic analysis of GSDM proteins.

Time-calibrated maximum clade credibility coalescent Bayesian phylogenetic tree of all protein sequences in selected superphyla by HMM identification method. Tree was visualized using iTOL v6 (Letunic and Bork, 2021).

Analysis of bird GSDMA sequences suggests caspase-1 cleavage.

(A.) Cartoons used in this study to represent organisms and their phylogenetic classification. Note that caecilian amphibians morphologically are similar to snakes in their lack of legs and elongated body, however, this is an example of convergent evolution as caecilians are amphibians and are thus more closely related to frogs and salamanders than snakes. (B.) Sequence logo plots derived from the tetrapeptides found in amphibian, bird and reptile GSDMA organized by evolutionary clades. Importantly, “Other birds” is not a monophyletic group. Sequence logos display abundance of amino acids in each position before an aspartic acid in the linker region of each GSDMA protein using WebLogo (Crooks et al., 2004). When more than one aspartic acids were present, multiple tetrapeptides were listed. (C.) Alignment of selected amphibian, reptile, bird and mammal GSDM linker regions by MUSCLE. Linker defined as region between β11 and α5 by SWISS-MODEL. Outlined in a red box are tetrapeptide sequences that precede caspase-1 cleavage sites for mammal, or show similarity in amphibian, reptile and bird sequences. The canonical caspase-1 cleavage site YVAD is emphasized with an arrowhead. “∗” denotes cleavage sites of SpeB for human and mouse GSDMA. Amino acids colorized using Clustal color scheme.

Chicken caspase-1 cleaves chicken GSDMA and mammal GSDMD.

(A.) Normalized relative GSDMA abundance by qRT-PCR using RNA derived from various Gallus gallus domesticus tissues. The cecal tonsils are a lymphoid aggregate tissue found in the chicken GI tract, akin to Peyer’s patches in mammals. ∗∗∗∗, p<0.0001 by 2 way ANOVA with Tukey’s multiple comparisons correction. (B.) Diagram of constructs used in this study. Note that CARD domain of caspase-1 is not present. (C.) FLAG blot of HEK293T/17 lysates after co-transfection of chicken GSDMA and caspase-1 after addition of DMSO or AP20187 dimerizer. (D.) FLAG blot of time course of dimerizer addition. Samples were harvested at 0, 0.25, 0.5, 0.75, 1, 1.5, 2, 2.5, 3, 4.5, and 6 hours after addition of AP20187 dimerizer. (E-H.) Caspase-1 from chicken, human and mouse cleave bird GSMDA and mammal GSDMD. FLAG blot of HEK293T/17 lysates after co-transfection with indicated caspase-1 and GSDM constructs. P1 refers to the fourth reside of the tetrapeptide sequence, which is cleaved by caspase-1. P1 mutants are chicken D244A, crow D243A, human D275A, and mouse D276A. (I.) FLAG blot of HEK293T/17 lysates after co-transfection with indicated gasdermin and caspase-1. Caspase-1 from chicken, human and mouse cleave human GSDMD but not human GSMDA. (J.) FLAG blot of HEK293T/17 lysates after co-transfection with indicated gasdermin and caspase-9. P1 mutant of chicken GSDME is 270DAVD273 to 270DAVA273.

Cleavage of chicken GSDMA leads to cell lysis.

(A.) Measurement of GFP fluorescence in HEK293T/17 cells co-transfected with GFP and either full length (FL) chicken GSDMA or its N-terminal (NT) pore-forming domain after 48 hours. Each point represents a separate transfection experiment. ****, p<0.0001 by student’s t-test. (B.) Cell lysis measured by LDH release in HEK293T/17 cells co-transfected with chicken caspase-1 and either WT or D244A chicken GSDMA. Gasdermin constructs in this study do not encode the 3xFLAG domain. Each point represents a separate transfection experiment. **, p<0.01; ****, p<0.0001 by 2 way ANOVA with Tukey’s multiple comparisons correction. (C.) Images of HeLa cells after transfection with the N-terminal domain of chicken GSDMA. Transfected cells display classic pyroptotic ballooning. SYTOX green staining of the nucleus indicates permeabilization of cell membranes.

Chicken GSDMA requires a compatible tetrapeptide for activation.

(A.) AlphaFold predictions of the structure of α7′ and α8 helices of human GSDMD and chicken GSMDA. α8 helices were aligned in Pymol. The human interhelix loop is indicated by the arrow. (B.) Gasdermin C-terminus AlphaFold predictions visualized with surface projection. Structures are aligned at the α8 helix. The arrow indicates the human interhelix loop and the space where human caspase-1 interacts with the C-terminal domain of human GSDMD (PDB: 6KN0, (Wang et al., 2020a)). (C.) Alignment of α7′-interhelix-α8 helix regions of human GSDMD (residues 347-380) and chicken GSDMA (residues 314-347). Residues colored with the Kyte and Doolittle scale with red indicating hydrophobic and blue indicating hydrophilic. “∗” indicates residues determined to be critical for the hydrophobic interaction between human GSDMD and caspase-1. (D.) FLAG blot of HEK293T/17 cell lysates after co-transfection with either chicken or human caspase-1 and their cognate GSDM with the tetrapeptide as WT, YVAD, or AAAD. (E.) Quantification of the intensity of the N-terminal band as a ratio of the N-terminal fragment over the full length, with the greatest ratio in a given experiment set to 100% efficiency. Each point is a blot from a separate transfection. a, p<0.05; b, p<0.0001; c, p<0.01 by 1 way ANOVA with Tukey’s multiple comparisons correction.

GSDMA from non-mammals are cleaved by caspase-1 and SpeB.

(A-C.) FLAG blot of HEK293T/17 lysates after co-transfection of A. mississippiensis, A. carolinensis, or M. unicolor GSDMA and caspase-1 from chicken, human and mouse. M. unicolor is an amphibian despite sharing morphological similarity to a snake. (D.) FLAG blot of HEK293T/17 lysates after co-transfection of platypus GSDMD or GSDMA with caspase-1 from chicken, human and mouse. (E.) FLAG blot of HEK293T/17 lysates after incubation with SpeB. Total protein captured by Ponceau staining for this blot.

Caspase-1 targets different gasdermins throughout evolution.

Phylogenetic tree indicating which GSDM caspase-1 cleaves in various clades of life. Branch lengths are not to scale. Coral, mollusks, hydras and other invertebrates do not encode caspase-1 and cleave GSDMin with caspase-3. Coral and mollusks are indicated in this diagram. We did not identify any gasdermins in 3,030 genomes from Ecdysozoa, the clade of which D. melanogaster and C. elegans are members. *Some teleost fish cleave GSDME with both caspase-1 and caspase-3, however, other teleost fish, like the zebrafish, cleave GSDMEb with caspase-1 and GSDMEa with caspase-3, gasdermins that arose after a whole genome duplication event in teleost fish. Frogs and salamanders do not encode an identifiable GSDMA, but their GSDME contains prototypical caspase-3 cleavage sites. In mammals, GSDMD is cleaved by caspase-1 and GSDME by caspase-3. In caecilian amphibians—which are morphologically similar to snakes—birds, and reptiles, GSDMA is cleaved by caspase-1. We and others have confirmed that GSDME in birds is cleaved by caspase-3 (Fig 3J, Fig 3-S2), and amphibian and reptile GSDME encodes prototypical caspase-3 cleavage sites.

Tree identical to Figure 1 with individual nodes labeled.

Tree identical to Figure 1 with individual nodes labeled and posterior probabilities displayed at the midpoint of each node.

Maximum likelihood tree generated using the same sequences in Figure 1 Bayesian tree.

Visualization of the locus where GSDMA is found in the clades of animals that encode this gasdermin. In tetrapods, the genes found in this region are identical, though ORMDL3 and LRRC3C are in opposing positions in some animals. Note that Microcaecilia unicolor (Tiny Cayenne caecilian) is an amphibian despite appearing morphologically similar to a snake. Duplications of GSDMA are indicated by numbers below GSDMA. Though not annotated as CSF3 in Anolis carolinensis (Carolina anole), the protein encoded by this sequence aligns with G-CSF (encoded by CSF3) in mammals by BLASTp. It is colored and labeled as CSF3 here for ease of appreciating synteny. In fish that encode GSDMEc, like Callorhinchus milii (elephant shark), GSDMEc is found in a similar locus near LRRC3Ca and MED24.

The same tissues as in Figure 3 were assayed for GSDME and CASP1 alongside GSDMA.

Incubation of 293T/17 lysates transfected with C-terminally tagged crow GSDMA, crow GSDME, human GSDMA, or human GSDMD then were incubated with human CASP1 or CASP3.

Amphibian expression of CASP1, GSDMA and GSDME.

Not all amphibians had RNA extracted from the same organs. Note that kidney, when isolated, always has the highest expression of GSDMA of non-gonadal tissue.

Two letter species abbreviations used in this study.

Plasmids used in this study.

Antibodies used for Western blotting in this study.

Selected primers used in this study.

Gasdermin sequences analyzed by AlphaFold.