Knockdown of FMNL2 disrupts actin assembly during mouse oocyte meiosis.
(A) Representative images of actin distribution at the oocyte cortex and the fluorescent intensities in the control group and FMNL2-KD group (P > 0.1). White, actin; green, tubulin; magenta, DNA. Bar = 10 μm. (B) Representative images of actin distribution in the oocyte cytoplasm and the fluorescent intensities in the control group and FMNL2-KD group. White, actin; green, tubulin; magenta, DNA. Bar = 10 μm. ****, significant difference (P < 0.0001). (C) Representative images of actin distribution in the oocyte cytoplasm and the fluorescent intensities in the FMNL2-KD group and rescue group. White, actin; magenta, DNA. Bar = 10 μm. ****, significant difference (P < 0.0001) (D) Mass spectrometry results showed that FMNL2 was related to many actin-related proteins. (E) Co-IP results showed that FMNL2 was correlated with Arp and Formin2 but not with Profiling and Fascin. (F) Arp2 protein expression significantly increased in the FMNL2-KD oocytes compared with the control oocytes. Arp2 protein expression significantly decreased in the rescue oocytes compared with the FMNL2-KD oocytes. **, significant difference (P < 0.01). (G) Formin2 protein expression significantly decreased in the FMNL2-KD oocytes compared with the control oocytes. Formin2 protein expression significantly increased in the rescue oocytes compared with the FMNL2-KD oocytes. **, significant difference (P < 0.01).