Figures and data
Heterozygous Kcnt1-Y777H expression alters AP shape and generation in NFS GABAergic neurons.
(A1-A3) On the left, representative responses to step currents are shown for glutamatergic, and FS and NFS GABAergic, WT (black) and YH-HET (colors) neurons (top to bottom), illustrating the input resistance (in response to a depolarizing step) and the rheobase (the first trace with an AP in response to a hyperpolarizing step) for each neuron type. On the right, bar graphs show quantification and mean ± SEM of the membrane properties and AP parameters for each neuron type for WT (grey) and YH-HET (colors) groups, with individual neuron measurements overlaid in scatter plots. The p-values are shown on each graph where p < 0.05. (B1-B3) Representative traces are shown at low, medium, and high current steps for glutamatergic, and FS and NFS GABAergic, WT (black) and YH-HET (colors) neurons (left to right). The line graphs below show the number of APs (mean ± SEM) per current injection step in WT (black) and YH-HET (colors) neurons. Statistical significance was tested using Generalized Linear Mixed Models, and p-values are shown on each graph where p < 0.05.
Heterozygous Kcnt1-Y777H expression increases subthreshold KNa currents in NFS GABAergic neurons.
(A1-A3) Representative traces in control (top), 0.5 µM TTX (middle), and the difference current (bottom) calculated by subtracting the membrane current response to voltage steps (-80 to +50 mV) from a holding potential of -70 mV in TTX from the response in control external solution in glutamatergic, and FS and NFS GABAergic, WT (black) and YH-HET (colors) neurons. To include all of the representative traces in the figures, and prevent overlap of the traces, the large inward sodium currents were removed from each set of traces using the masking tool in Adobe Illustrator. (B1-B3) Summary data shows the KNa current (mean ± SEM) for each voltage step in glutamatergic, and FS and NFS GABAergic, WT (black and gray) and YH-HET (colors) neurons. The p-values are shown on each graph where p < 0.05, and the n values are the number of neurons recorded for each group. (C1-C3) Plots of the KNa current (mean ± SEM) for each voltage step from -80 to 0 mV in WT (black and gray) and YH-HET (colors) neurons to illustrate the values that are too small to be seen on the graphs in B1-B3. The shaded red area in C3 indicates the subthreshold voltage range with significantly higher KNa currents (red voltages along x-axis indicate steps where p < 0.05) in YH-HET relative to WT neurons. The p-values are shown on each graph where p < 0.05, and the n values are the number of neurons recorded for each group. Statistical significance for I-V plots was tested using Generalized Linear Mixed Models with genotype and voltage step as fixed effects followed by pairwise comparisons at each level.
Heterozygous Kcnt1-Y777H expression does not alter synaptic connectivity or the excitation-inhibition balance.
(A1-A4) Evoked postsynaptic currents (PSCs) were recorded from neuron pairs [glutamatergic (excitatory, E) and GABAergic (inhibitory, I)] by stimulating the neuron type indicated on the left and recording the response in the neuron type indicated on the right (WT, gray; YH-HET E, green; YH-HET I, red). Bar graphs below each recorded neuron pair schematic show summary data (mean ± SEM) of the connection probability (left graph; numbers on bars represent connected pair number/recorded pair number) and peak evoked PSC amplitude (right graph; dots represent individual evoked responses) between each motif. (B1) Bar graphs with overlaid individual neuron measurements and mean ± SEM show the spontaneous EPSC (sEPSC) or IPSC (sIPSC) frequency onto E neurons (WT, gray; YH-HET, green). (B2) Bar graphs with overlaid individual neuron measurements and mean ± SEM show the sEPSC or sIPSC frequency onto I neurons (WT, gray; YH-HET, red). (B3) Scatter plots show individual E/I ratio measurements onto E neurons (WT, gray; YH-HET, green) and I neurons (WT, gray; YH-HET, red). The p-values are shown on each graph where p < 0.05.
Heterozygous Kcnt1-Y777H expression differentially affects the intrinsic excitability of SST- and PV-expressing GABAergic neurons.
(A) A schematic diagram illustrates the strategy for generating fluorescently labeled GABAergic subtype-specific neurons. YH-HET mice were crossed to VIP-, SST-, or PV-Cre mice, and the resulting P0 WT and YH-HET littermate pups were used to isolate and culture cortical neurons. At DIV 1, neurons were infected with AAV-CamKII-GFP to label glutamatergic neurons, and AAV-hSyn-DIO-mCherry to label Cre-expressing neurons. At DIV 13–17, whole-cell, patch-clamp electrophysiology was performed on mCherry+/GFP- neurons. (B1-B3) On the left, representative responses to step currents are shown for VIP-, SST-, and PV-expressing WT (black) and YH-HET (colors) neurons (top to bottom). For each neuron type, the superimposed dark traces illustrate the input resistance (in response to a depolarizing step) and the rheobase (the first trace with an AP in response to a hyperpolarizing step), and the light trace shows the first step current response to induce repetitive AP firing across the step. Above the superimposed traces, the first AP of each rheobase trace is shown (same vertical scale, increased horizontal scale). On the right, bar graphs show quantification of the membrane properties and AP parameters for each neuron type (VIP, SST, and PV, top to bottom) for WT (grey) and YH-HET (colors) groups, with individual neuron measurements overlaid in scatter plots. The p-values are shown above each graph where p < 0.05. (C1-C3) For VIP-, SST-, and PV-expressing neurons (left to right), representative traces are shown at low, medium, and high current steps, and the line graphs below show the number of APs (mean ± SEM) per current injection step in WT (black) and YH-HET (colors) neurons. Statistical significance was tested using Generalized Linear Mixed Models, and p-values are shown above each graph where p < 0.05.
The Kcnt1-Y777H variant increases KCNT1-mediated currents across subthreshold voltages in SST- and PV-expressing GABAergic neurons.
(A1-A3) Representative traces in control (top), 10 µM VU170 (middle), and the difference current (bottom) calculated by subtracting the membrane current response to voltage steps (-80 to +50 mV) from a holding potential of -70 mV in VU170 from the response in control external solution in VIP-, SST-, and PV-expressing, WT (black) and YH-HET (colors) neurons. To include all of the representative traces in the figures, and prevent overlap of the traces, the large inward sodium currents were removed from each set of traces using the masking tool in Adobe Illustrator. (B1-B3) Summary data shows the KCNT1 current (mean ± SEM) for each voltage step in VIP-, SST-, and PV-expressing, WT (black and gray) and YH-HET (colors) neurons. The p-values are shown on each graph where p < 0.05, and the n values are the number of neurons recorded for each group. (C1-C3) Plots of the KNa current (mean ± SEM) for each voltage step from -80 to 0 mV in WT (black and gray) and YH-HET (colors) neurons to illustrate the values that are too small to be seen on the graphs in B1-B3. The shaded red areas in C2 and C3 indicate the subthreshold voltage range with significantly higher KNa currents (p < 0.05) in YH-HET relative to WT neurons. Statistical significance for I-V plots was tested using Generalized Linear Mixed Models with genotype and voltage step as fixed effects followed by pairwise comparisons at each level.
Compartmental models of KCNT1 GOF in SST, but not PV, neurons are consistent with experimental data.
(A) Activation curves for VIP, SST, and PV neurons used to model the KCNT1 channel in each neuron type. (B) Representative traces of the response of an outside-out membrane patch pulled from an SST neuron in control external solution (top) and after application of 10 µm VU170 (bottom). (C) Representative trace of the VU170-sensitive current in an outside-out patch pulled from an SST neuron with a single exponential fit (red curve) overlaid and summary data showing the time constant (mean ± SEM) obtained from these fits. (D) Simulated traces showing the KCNT1-mediated current in model neurons at three levels of Na+-sensitivity (Con-40 mM, GOF-35 mM, and GOF-30 mM). (E1, F1, and G1) Simulated traces from model VIP (E1; purple), SST (F1; blue), and PV (G1; yellow) neurons in response to 500-ms current injections at three levels of Na+-sensitivity [control (Con), and two levels of KCNT1 GOF]. Representative traces are shown at increasing current steps from bottom to top for each level. (E2, F2, and G2) Summary data from 10 model VIP (E2; purple), SST (F2; blue), and PV (G2; yellow) neurons showing (from left to right) the number of APs at different current steps (F-I plot), the input resistance, and rheobase. The bar graphs (mean ± SEM) are overlaid with scatter plots of individual neuron measurements. Statistical significance was tested using a Repeated Measures ANOVA. Asterisks indicate where p < 0.05 (*), p ≤ 0.01 (**), and p ≤ 0.001 (***).
The persistent Na+ current is increased by the Kcnt1-Y777H variant in PV, but not SST, neurons.
(A) Representative traces of WT (left) and YH-HET (right) SST neurons in response to slow voltage ramp in control (dark gray) and 7 seconds after application of 0.5 nM TTX (light gray) with the difference current (INaP) plotted on the same scale. (B) INaP I-V curves of WT (gray) and YH-HET (blue) SST neurons constructed from averaging the ramp-evoked difference current (mean ± SEM) at 5-mV intervals (left), and a bar graph with overlaid scatter plot showing the peak negative value for each neuron (right). (C) Representative traces of WT (left) and YH-HET (right) PV neurons in response to slow voltage ramp in control (dark gray) and 7 seconds after application of 0.5 nM TTX (light gray) with the difference current (INaP) plotted on the same scale. The upper traces and difference currents are plotted on different scales. (D) INaP I-V curves of WT (gray) and YH-HET (orange) PV neurons constructed from averaging the ramp-evoked difference current (mean ± SEM) at 5-mV intervals (left), and a bar graph with overlaid scatter plot showing the peak negative value for each neuron (right). (E) Simulated traces from a model PV neuron in response to 500-ms current injections with control levels of KCNT1 (black traces) and KCNT1 GOF (Na+ EC50 = 35 mM) with a 2-fold increase in INaP (orange traces). Representative traces are shown at increasing current steps from bottom to top for each level. (F) F-I plot shows the increase in AP firing (left), and a bar graph with overlaid scatter plot of individual neuron values shows the decrease in rheobase associated with modeling KCNT1 GOF (Na+ EC50 = 35 mM) together with the increase in INaP. For modeling data, statistical significance was tested using a Repeated Measures ANOVA. Asterisks indicate where p < 0.05 (*), p ≤ 0.01 (**), and p ≤ 0.001 (***).
YH-HET SST neurons show increased chemical and electrical coupling and receive increased excitatory input.
(A and B) Evoked postsynaptic currents (PSCs) were recorded from SST neuron pairs by stimulating each neuron at 0.1 Hz and recording the response onto the partner. (A) A bar graph shows the connection probability (mean + SEM) between SST neurons (WT, gray; YH-HET, blue; numbers on bars represent connected pair number/recorded pair number). (B) On the left, example traces of evoked IPSCs [individual IPSCs (light) overlaid by averaged IPSCs (dark)], and on the right, a graph of connection strength between SST neurons (WT, gray; YH-HET, blue; individual measurements and summary violin plots). (C1) A schematic illustrates representative responses (presynaptic, black; postsynaptic, red) between chemically coupled (upper two panels) and electrically coupled (lower two panels) SST neurons following 100-pA (left two panels) and AP (right two panels) stimulation. (C2) Summary data of four possible connection motifs (not coupled, chemical: one-way, chemical: two-way, and electrical) tested among WT (gray box) and YH-HET (blue box) SST neuron pairs (20 pairs/group). (D) On the left, example traces of spontaneous EPSCs (sEPSCs) recorded onto SST neurons (WT, black; YH-HET, blue). On the right, a graph shows individual neuron measurements and summary violin plots of the sEPSC frequency onto SST neurons (WT, gray; YH-HET, blue). All significant p-values are displayed at the top of each graph.
Acute cortical slice recordings confirm the reduced intrinsic excitability of YH-HET SST neurons.
(A) An inverted monochrome image illustrates the recording scheme from GFP-expressing neurons in acute slices from P21–P30 GIN mice that express GFP in a subset of SST-positive neurons. (B) Representative responses to current steps are shown for WT (black) and YH-HET (blue) to three, equivalent 1 s current steps. (C) Plot of the action potential frequency (mean ± SEM) per current injection step shows that YH-HET GFP-expressing neurons (blue) fire fewer action potentials than those of WT (grey) across a range of current inputs. (D) Bar graphs show quantification of the membrane properties and AP parameters for WT (grey) and YH-HET (colors) GFP-expressing neuron groups, with individual neuron measurements overlaid in scatter plots.
The magnitudes of the KNa current increases in YH-HET glutamatergic and NFS GABAergic neurons are intermediate to those of WT and YH-HOM neurons.
Bar graphs (top three) illustrate differences in KNa current (mean ± SEM) between YH-HET [dark color; WT littermates (black)] and YH-HOM [light color; WT littermates (gray)] neurons for voltage steps where significant increases were previously observed (red asterisks where p < 0.05) in glutamatergic, and FS and NFS GABAergic, YH-HOM neurons. Line graphs (bottom three) illustrate differences in KNa current (mean ± SEM) between YH-HET [dark color; WT littermates (black)] and YH-HOM [light color; WT littermates (gray)] neurons across negative voltage steps (-80 to -20 mV; red asterisks where p < 0.05 in YH-HOM compared with WT) in glutamatergic, and FS and NFS GABAergic, neurons.
Cre recombinase-expressing, cortical GABAergic neuron subpopulations retain characteristic electrophysiological features in culture.
Cortical neurons isolated and cultured from P0 pups (Kcnt1+/+) from VIP-, SST-, or PV-Cre mouse lines were infected with AAV-CamKII-GFP to label glutamatergic neurons, and AAV-hSyn-DIO-mCherry to label Cre-expressing neurons, at DIV 1. (A) Images show GFP+ glutamatergic neurons and mCherry+ VIP-, SST-, or PV-expressing neurons (left to right) at DIV 14 (VIP and SST) or DIV 16 (PV). The scale bars represent 50 µm. (B) Representative responses to step currents are shown for VIP (purple), SST (blue), and PV (orange) neurons (left to right). For each neuron type, the superimposed dark traces illustrate the input resistance (in response to a depolarizing step) and the rheobase (the first trace with an AP in response to a hyperpolarizing step), and the light trace shows the first step current response to induce repetitive AP firing across the step. (C) On the left, the first AP of each rheobase trace is shown for VIP (purple), SST (blue), and PV (orange) neurons. On the right, bar graphs show quantification of the membrane properties and AP parameters for each neuron type (VIP, SST, and PV, left to right), with individual neuron measurements overlaid in scatter plots.
YH-HET SST neurons have increased soma size compared with those of WT.
Cortical neurons were isolated and cultured from P0 pup progeny (WT and YH-HET) of YH-HET mice crossed to SST-Cre mice. Neurons were infected with AAV-CamKII-GFP to label glutamatergic neurons, and AAV-hSyn-DIO-mCherry to label Cre-expressing neurons, at DIV 1, and immunostained using anti- Neu-N antibodies at DIV 16. (A) Top two images show mCherry+ SST-expressing neurons as a subpopulation of Neu-N+ cortical neurons, and bottom two images show GFP-, mCherry+ SST-expressing neurons intermingled with GFP+ glutamatergic neurons. The scale bars represent 50 µm. (B) A graph shows individual neuron measurements and summary violin plots of the mCherry+ neuron soma size (WT, gray; YH-HET, blue) with the p-value displayed at the top.
YH-HET and WT PV neurons show similar neurophysiological responses to increased temperature.
YH-HET mice were crossed to PV-Cre mice, and the resulting P0 WT and YH-HET littermate pups were used to isolate and culture cortical neurons. At DIV 1, neurons were infected with AAV-CamKII-GFP to label glutamatergic neurons, and AAV-hSyn-DIO-mCherry to label Cre-expressing neurons. At DIV 16 and 17, whole-cell, patch-clamp electrophysiology was performed on glutamatergic (mCherry-/GFP+), GABAergic (mCherry-/GFP-), or PV (mCherry+/GFP-) neurons at room temperature (∼22°C). Then, the external solution was warmed to 35°C, and the measurements were repeated. (A) Before-after plots of glutamatergic and GABAergic WT neurons (n=16) show alterations in all parameters measured with increased temperature, excluding the resting membrane potential (Vrest) and membrane capacitance (Cm). (B) Before-after plots show highly similar responses to temperature increases between WT (n=11) and YH-HET (n=10) PV neurons for rheobase, AP amp, and maximal firing frequency (MFR). Empty circles represent individual neurons, and filled-in circles represent the averages for each group. Lines connect the before and after measurements for each neuron.
The Kcnt1-Y777H variant increases KNa currents measured with TTX subtraction across subthreshold voltages in SST-expressing GABAergic neurons. (A1-A3)
Representative traces in control (top), 0.5 µM TTX (middle), and the difference current (bottom) calculated by subtracting the membrane current response to voltage steps (-80 to +50 mV) from a holding potential of -70 mV in TTX from the response in control external solution in VIP-, SST-, and PV-expressing, WT (black) and YH-HET (colors) neurons. To include all of the representative traces in the figures, and prevent overlap of the traces, the large transient inward sodium currents were removed from each set of traces using the masking tool in Adobe Illustrator. (B1-B3) Summary data shows the KNa current (mean ± SEM) for each voltage step in VIP-, SST-, and PV-expressing, WT (black and gray) and YH-HET (colors) neurons. The p-values are shown on each graph where p < 0.05, and the n values are the number of neurons recorded for each group. (C1-C3) Plots of the KNa current (mean ± SEM) for each voltage step from -80 to 0 mV in WT (black and gray) and YH-HET (colors) neurons to illustrate the values that are too small to be seen on the graphs in B1-B3. The shaded red area in C2 indicates the subthreshold voltage range with significantly higher KNa currents in YH-HET relative to WT neurons. Statistical significance for I-V plots was tested using Generalized Linear Mixed Models with genotype and voltage step as fixed effects followed by pairwise comparisons at each level.
Isolation of KNa currents from neurons lacking KCNT1 and KCNT2 channels illustrates the specificity of VU170- vs. TTX-subtraction methods.
Voltage steps were applied to voltage-clamped neurons isolated and cultured from Kcnt1-/-; Kcnt2-/- double knockout mice to compare the delayed outward currents before and after the addition of either 10 µM VU170 or 0.5 µM TTX. (A) A summary plot shows the VU-sensitive (red) and TTX-sensitive (grey) difference currents (mean ± SEM) for each voltage step, which were calculated by subtracting the membrane current response to voltage steps (-80 to +50 mV) from a holding potential of -70 mV in VU170 or TTX from the response in control external solution. (B) A summary plot of the VU-sensitive (red) and TTX-sensitive (grey) difference currents (mean ± SEM) for each voltage step from -80 to 0 mV to illustrate the values that are too small to be seen on the graph in (A).
Model VIP and glutamatergic neurons with insertion of SST-like, KCNT1 GOF kinetics show reduced excitability. (A1)
Simulated traces from model VIP neurons, using VIP KCNT1 activation curve parameters (purple) or SST KCNT activation curve parameters (blue), in response to 500-ms current injections at two levels of Na+-sensitivity (Con-40 mM and GOF-30 mM). Representative traces are shown at increasing current steps from bottom to top for each level. (A2) Summary data from 10 model VIP neurons, with VIP KCNT1 parameters (VIP-Con and VIP-GOF) or SST KCNT1 parameters (SST-GOF) showing (from left to right) the number of APs at different current steps (F-I plot), the input resistance, and rheobase. The bar graphs (mean ± SEM) are overlaid with scatter plots of individual neuron measurements. (B1) Simulated traces from model glutamatergic neurons, using glutamatergic KCNT1 activation curve parameters (green) or SST KCNT activation curve parameters (blue), in response to 500-ms current injections at two levels of Na+-sensitivity (Con-40 mM and GOF-30 mM). Representative traces are shown at increasing current steps from bottom to top for each level. (B2) Summary data from 10 model glutamatergic neurons, with glutamatergic KCNT1 parameters (Glut-Con and Glut-GOF) or SST KCNT1 parameters (SST-GOF) showing (from left to right) the number of APs at different current steps (F-I plot), the input resistance, and rheobase. The bar graphs (mean ± SEM) are overlaid with scatter plots of individual neuron measurements. Statistical significance was tested using a Repeated Measures ANOVA. Asterisks indicate where p < 0.05 (*), p ≤ 0.01 (**), and p ≤ 0.001 (***).
Model neurons show differential responses to KCNT1 GOF.
(A) Bar graphs (mean ± SEM) with overlaid dot plots show the effects of two levels of KCNT1 GOF (Na+ EC50 = 35 or 30 mM) relative to those of control (EC50 = 40 mM) on input resistance, rheobase, and AP firing rate at 1.5 × rheobase for GABAergic subtypes (10 model neurons/group). (B) Bar graphs (mean ± SEM) with overlaid dot plots show the effects KCNT1 GOF (Na+ EC50 = 30 mM) on VIP GABAergic or glutamatergic model neurons (10/group) with their respective KCNT1 activation curve parameters (VIP-GOF and Glut-GOF) or SST KCNT1 parameters (SST-GOF) relative to those of control (EC50 = 40 mM) on input resistance, rheobase, and AP firing rate at 1.5 × rheobase.
The rate of KNa current block after TTX application is slow relative to INaP block in GABAergic neurons.
Plots show the time course over which INaP and KNa currents are blocked following 0.5 µM TTX application. Neurons of the labeled type in each panel were voltage clamped and subjected to a slow voltage ramp protocol (20 mV/s over 5 s). TTX (0.5 µM) was applied at time 0 and KNa currents and INaP were measured on each successive sweep. The KNa current was measured as the normalized difference between each trace and the mean of the pre-TTX current between +20 and +30 mV. INaP was measured as the normalized difference between each trace and the mean of the pre-TTX current at the 10 mV bracketing the local peak inward current. Once the minimum value was reached for INaP, all subsequent values were considered zero.
Model PV neurons show differential responses to KCNT1 GOF with increased INaP.
Bar graphs (mean ± SEM) with overlaid dot plots show the effects of two levels of KCNT1 GOF (Na+ EC50 = 35 or 30 mM), with or without additional INaP, relative to those of control (EC50 = 40 mM) on input resistance, rheobase, and AP firing rate at 1.5 × rheobase for PV neurons (10/group).
Electrophysiological parameters of current clamp recordings from neuronal cultures.
Electrophysiological parameters of current clamp recordings from neuronal cultures of three major GABAergic subtypes.
