Introduction

There has been an explosion in the use of imaging techniques to record neuronal activity over the past 30 years, starting with the introduction of organic calcium indicators to measure neuronal population activity (Yuste & Katz 1991) and accelerated by rapid advances in the development of genetically encoded Ca²⁺ indicators (GECIs) (Miyawaki et al. 1997). Specific advantages of neuronal Ca²⁺ imaging with GECIs lie in the ability of chronic cellular scale recordings of sizeable, densely labelled neuronal or glial populations with subtype specificity, without having to perturb the cell membrane or add synthetic chemical to the brain (Grienberger & Konnerth 2012; Rose et al. 2014; Semyanov et al. 2020).

Commonly used GECIs such as the GCaMP family have been continually improved since their initial development (Nakai et al. 2001), offering high signal to noise ratio, sensitivity and response kinetics such that they can detect single action potentials in vivo. This allows the reporting of cellular activity as well as the activity of sub-compartments such as the dendritic arbour (Chen et al. 2013; Dana et al. 2019; Zhang et al. 2023). Typically, GCaMP is expressed using transgenic animals or adenoviral (AAV) transduction techniques (Tian et al. 2012). The use of transgenic animals has the advantage of not requiring AAV transduction, thus reducing surgery load for animals and likelihood of indicator overexpression. In contrast, adenoviral GECI transduction is straightforward (breeding/crossing not required), can be targeted to virtually any brain region, and typically offer enhanced fluorescence (due to higher expression levels). Further, AAV transduction avoids potential hazards due to chronic GECI expression during development.

While offering unprecedented new insights into cellular scale neuronal network dynamics, it has also been reported in recent years that GECI expression in neurons can result in unwanted side-effects. Depending on the expression approach, neurons have shown reduced dendritic branching and impairment in cell health leading to cytotoxicity and cell death (Gasterstädt et al. 2020; Resendez et al. 2016). Further, increased Ca²⁺ buffering due to addition of Ca²⁺ indicators has been associated with alterations in intracellular Ca²⁺ dynamics (Grienberger & Konnerth 2012; McMahon & Jackson 2018). In addition, chronic expression of GCaMP can lead to accumulation in the nucleus and changes in gene expression (Yang et al. 2018). Again, depending on the specific expression approach, GCaMP variant and experimental time course, such changes may alter cellular physiology and excitability. For example, increased firing rates have been observed in hippocampal neurons expressing GCaM5G from CaMKIIa-Cre; PC::G5-tdT mice, and epileptiform activity in neocortex in some GCaMP6 expressing transgenic mice (Gee et al. 2014; Steinmetz et al. 2017).

Here, we describe micro-scale Ca²⁺ waves that are highly confined in space, and progress slowly through the hippocampus following local GCaMP or R-CaMP viral transduction. Such aberrant hippocampal waves were typically first observed 4 weeks following injection of commercially available AAVs expressing GCaMP6, GCaMP7 or R-CaMP1.07 under the synapsin promoter. The phenomenon occurred upon GECI transduction in CA1 and CA3, but not in dentate gyrus (DG) nor neocortex, was robustly observed across laboratories with various experimenters and set-ups, and highlights the necessity of careful use of transduction methods and control measures. Reducing the transduction titre diminished the likelihood of aberrant hippocampal Ca²⁺ waves, and an alternative viral transduction method employing sparser and cre-dependent GCaMP6s expression in principal cells avoided the aberrant Ca²⁺ waves. Further, in three transgenic Thy1-GCaMP mouse lines (thy1-GCaMP6s or 6f; Vglut1-IRES2-Cre-D x Ai162(TIT2L-GC6s-ICL-tTA2), aberrant Ca²⁺ micro-waves were never observed. The aim of this article is to raise awareness in the field of artefactual transduction-induced Ca²⁺ waves and encourage others to carefully evaluate their Ca²⁺ indicator expression approach before embarking on chronic in vivo calcium imaging of the hippocampus.

Results

Aberrant Ca²⁺ micro-wave progression through hippocampus

We injected AAV1 particles (pAAV.Syn.GCaMP6s.WPRE.SV40, Addgene #100843, titre 1×10¹³ vg/ml) in the hippocampus (total injection vol.: 500nl undiluted [1:1] virus solution) of C57BL6 wild-type animals (6 weeks old), and performed in vivo two-photon imaging to record cellular activity at 2, 4 and 6-8 weeks (wks) post-injection (p.i.) (Fig. 1a). Viral transduction resulted in GCaMP6s expression throughout the hippocampal CA1, CA3 and DG areas under the imaging window (Fig. 1b). As expected, the expression was primarily restricted to the ipsilateral hippocampus, with some labelling of projection pathways also in the contralateral hippocampus. At 4 wks after injection, a time point commonly used for imaging cellular activity, we observed distinctive aberrant microscale Ca²⁺ waves that travelled through CA1 recruiting neighbouring cells (Fig. 1c-d, Supplementary video 1, n=4 mice). Ca²⁺ micro-waves were maintained up to 6-8 wks after AAV injections (Fig. 1e, n=4 mice). These Ca²⁺ micro-waves were not observed at an earlier timepoint (2 wks p.i).

Figure 1:

The properties of the Ca²⁺ micro-waves depended on the hippocampal region and exact recording location. For instance, although the Ca²⁺ waves were consistently observed in CA1, the spatial dimensions of the Ca²⁺ micro-waves were broader in the stratum oriens compared with stratum pyramidale of CA1 (Supplementary videos 4 [str. pyr.] and 5 [str. oriens]). We next examined, if the CA1 network is particularly prone to the generation of such waves and if they show regional specificity. Upon viral syn-GCaMP6s transduction, Ca²⁺ waves were observed in both CA1 (Supplementary videos 1-5) and CA3 (n=1; Supplementary video 6), but interestingly, not in the dentate gyrus (n=3 mice, 4, 8 & 10 wks p.i., 40 min total recording time per mouse). In contrast to hippocampus, syn-GCaMP6s expression restricted to the neocortex did not result in cortical Ca²⁺ waves in our hands (Fig. 1f, n = >20 mice).

Aberrant Ca²⁺ microwaves in disease models

The observed Ca²⁺ micro-waves were distinct from local seizure activity (no rhythmicity, no typical ictal evolution, no postictal depression) (Masala et al. 2022; Muldoon et al. 2015; Wenzel et al. 2017, 2019a) and spreading depolarization/depression phenomena (no concentric expansion, no post-wave neural depression). However, the occurrence of these artificial events may be confused as aberrant activity related to a pathology, especially when studying pathologies with known cellular and network hyperexcitability. For example, we initially found the aberrant hippocampal Ca²⁺ micro-waves in the Scn2a^A263V model of genetic epilepsy; however, these Ca²⁺ waves in CA1 of heterozygous animals (5/5 mice) were in general similar to those detected in wildtype animals at 4 wks p.i.. Only in a rare case (1/5 animals) were Ca²⁺ waves observed at 2 wks p.i. in the Scn2a^A263V model (Supplementary video 2). Furthermore, hippocampal transduction of syn-jGCaMP7f (Addgene #104488, AAV9 particles, original titre 2.5×10¹³ vg/ml, total inj. vol. 1000nl [1:2 dilution]) in a mouse model of Alzheimer’s disease (PV-Cre::APPswe/PS1dE9) also resulted in Ca²⁺ micro-waves (n=3/6 mice). Together, these experiments show that common AAV injection procedures of GECIs under the synapsin promoter lead to artefactual hippocampal Ca²⁺ micro-waves in wildtype mice and genetic mouse models of disease.

Properties & robustness of aberrant hippocampal Ca²⁺ waves

Next, we investigated the robustness of the aberrant Ca²⁺ micro-waves across institutes and conditions. We chose to compare the incidence of aberrant Ca²⁺ micro-waves in the CA1 region in four separate institutes in three different countries following transduction of GCaMP6s (Addgene #100843; IEECR/UoB, CU), GCaMP6m or jGCaMP7f (Addgene #100841 or #104488; DZNE) or RCaMP1.07 (Viral Vector Facility UZH #V224-9; UZH, Supplementary video 3)(Table 1).

Table 1:

The incidence of aberrant hippocampal Ca²⁺ micro-waves was robust, observed at the 4 different institutes each using variations of commonly used viral transduction procedures and standard two-photon Ca²⁺ imaging protocols (Table 1 and see methods for more details). The viral titre, however, was important as halving the original syn-GCaMP6m viral titre decreased the number of animals that developed aberrant Ca²⁺ micro-waves from 80% of animals (4/5, original titre, 1×10¹³ vg/mL) to 43% of animals (3/7, 50% reduced titre, 0.5×10¹³ vg/ml) (see Table 1).

To examine how robust the Ca²⁺ micro-waves properties were, we compared properties across the different laboratories following expression of GCaMP6m and GCaMP6s variants. The occurrence rate of the aberrant Ca²⁺ micro-waves was similar across the different institutes (Fig. 2a). Ca²⁺ micro-waves were spatially confined (diameter range of 200-300 µm), moved across the field of view with slow progression speeds (speed range of 5-25 µm/sec), (Fig. 2b), and displayed no rhythmicity but rather plateau-like Ca²⁺ activity. In addition, the Ca²⁺ micro-waves were not restricted to a single GECI variant or version with Ca²⁺ waves observed following expression of GCaMP6m (n=4), GCaMP6s (n=5), and GCaMP7f (n=3), as well as following expression of R-CaMP1.07 (n=1)(Fig. 2c and Table1).

Fig. 2

In summary, upon synapsin-promoter-dependent adenoviral Ca²⁺ indicator expression, depending on time of expression and viral transduction titre, Ca²⁺ micro-waves were specifically observed in the CA1 and CA3 subregions of the hippocampus, across laboratories and countries and animal models, using common transduction procedures (for overview see Table 1).

Alternative transduction method of GCaMP to avoid aberrant Ca²⁺ micro-waves

In light of these results, we tested an alternative expression approach to avoid aberrant hippocampal Ca²⁺ micro-waves. To this end, we selected an approach to both limit the expression to principal cells and label a sparse population of the principal cells using a dual AAV injection approach. Here, cre-dependent expression of syn-GCaMP6s was achieved in a sparse population of principal cells under the CaMKII promoter (AAV1.syn.Flex.GCaMP6s.WPRE.SV40, Addgene #100845, and AAV1.CamKII0.4.Cre SV40, Addgene #105558; Fig. 2d) (see Sheffield & Dombeck 2015), upon which no Ca²⁺ micro-waves were observed (0/2 animals, Fig. 2d). Further, hippocampal Ca²⁺ micro-waves were neither observed in transgenic thy1-GCaMP6s nor 6f mice (JAX strain 025776 or 024276; up to 3 months of chronic imaging in n>30 mature mice age >p60, cumulative imaging time >200hrs) nor in Vglut1-IRES2-Cre-D mice crossed with Ai162(TIT2L-GC6s-ICL-tTA2)-D mice (JAX strains: 037512, 031562; up to 3 months of chronic imaging in n=5 mature mice >p60).

Discussion

Here we report titre-and expression-time-dependent aberrant hippocampal Ca²⁺ micro-waves in CA1 and CA3 regions following viral expression of syn-GCaMP or syn-R-CaMP1.07. These aberrant Ca²⁺ micro-waves robustly occurred, were observed in 4 different institutes each using a common viral transduction approach and standard two-photon Ca²⁺ imaging protocols.

Ca²⁺ micro-waves were typically first detected at ∼4wks, rarely also at 2 wks, after injection. Thus, there may be a time window when Ca²⁺ activity could be recorded in the absence of this artefactual phenomenon. However, we would still hesitate to use this specific approach for imaging experiments, as more subtle alterations likely occur prior to visible onset of aberrant activity. Further, at sites more distal to the injection site with lower expression levels, Ca²⁺ micro-waves may not be observed; however, it may very well be that Ca²⁺ micro-waves in regions with higher expression will affect fine-scaled neuronal population dynamics in primarily unaffected neighbouring regions.

The presence of Ca²⁺ micro-waves was not restricted to a single GCaMP variant or version, and was observed using either GCaMP6m, GCaMP6s, or GCaMP7f. The phenomenon was also observed upon transduction of R-CaMP1.07, indicating that these aberrant hippocampal waves are not restricted to GCaMP indicators, but rather present a general phenomenon following syn-Ca²⁺-indicator transduction. However, the viral transduction titre was a key factor, as reducing the viral transduction titre from 1×10¹³ vc/ml (500nl or 1000nl of a 1:1 undiluted virus solution) to 5×10¹² vc/ml (1000nl 1:2 solution, single injection) decreased, albeit did not yet prevent, the occurrence of Ca²⁺ micro-waves. In the literature, hippocampal syn-GCaMP transduction procedures in mice typically include one to several separate nearby injections, with a total volume of transduced undiluted virus ranging from 60nl to 500nl (Cai et al. 2016; Jimenez et al. 2020; Keinath et al. 2022; Pettit et al. 2022; Radvansky et al. 2021; Skocek et al. 2018; Szabo et al. 2022; Weisenburger et al. 2019; Wirtshafter & Disterhoft 2022; Zaremba et al. 2017). In other studies, syn-GCaMP virus was diluted prior to injection (up to 1:10)(Jimenez et al. 2020; Zong et al. 2022), resulting in varied transduction volumes up to 1500nl. In our hands, a reduced viral titre of 5×10¹² vc/ml in a 1000nl injection volume still resulted in aberrant Ca²⁺ micro-waves. Thus viral transduction titres per volume well below this number and diluted transduction solutions are advisable for syn-GCaMP expression in the hippocampus, if adenoviral syn-Ca²⁺- indicator transduction is desired for a planned in vivo hippocampal imaging experiment.

If possible, alternate viral GCaMP expression approaches should be chosen. As a possible alternative, similar to previous reports using dual AAV injections or AAV in Cre-driver mouse lines (Farrell et al. 2020; Grosmark et al. 2021; Mineur et al. 2022; Rolotti et al. 2022; Terada et al. 2022), we find that cre-dependent adenoviral GCaMP expression (IEECR/UoB) in pyramidal neurons does not cause aberrant hippocampal Ca²⁺ micro-waves. Moreover, we have not observed this aberrant phenomenon in transgenic thy1-GCaMP6s or 6f mice (JAX strain 025776 or 024276)(Masala et al. 2022; Rupprecht et al. 2023; Wenzel et al. 2019b), nor in Vglut1-IRES2-Cre-D x Ai162(TIT2L-GC6s-ICL-tTA2)-D mice (JAX strains: 037512, 031562). It goes beyond the scope and available resources in our laboratories to further identify, which viral GCaMP transduction approaches avoid the reported phenomenon. It seems likely that the underlying mechanisms for this artefact comprise not only transduction titre and time period of GCaMP or R-CaMP1.07 expression, but at least also density and cell type of expression. It remains unclear if the observed phenomenon is restricted to hippocampal syn-Ca²⁺ indicator viral expression in mice, or if it extends to different animal models as well. In this regard, a previous report did not observe Ca²⁺-waves in rats following syn-GCaMP6m expression, although imaging was performed under isoflurane anaesthesia (Sosulina et al. 2021). Importantly, expression of GCaMP under the synapsin promoter per se does not necessary triggers the phenomenon, as cre-dependent GCaMP expression under the synapsin promoter in pyramidal neurons failed to cause Ca²⁺ micro-waves.

The phenomenon of Ca²⁺ micro-waves is possibly related to the expression of exogenous Ca²⁺ buffer and the resulting effects on Ca²⁺ dynamics and gene expression (McMahon & Jackson 2018; Rose et al. 2014; Yang et al. 2018), which may be why our findings extended across genetically encoded Ca²⁺ indicators. Beyond clearly being abnormal, the exact nature of the observed Ca²⁺ micro-waves remains unclear, and identifying the precise mechanisms underlying these waves lies beyond the scope of this article, especially as it remains unknown if this phenomenon has any physiological or pathophysiological relevance. Certainly, none of the researchers who contributed to this article have observed Ca²⁺ waves with the described spatiotemporal features under healthy or pathophysiological conditions, except when syn-GCaMP or syn-R-CaMP1.07 was expressed as described here.

In summary, this report shows that common AAV hippocampal injection procedures of syn-Ca²⁺ indicators may lead to aberrant Ca²⁺ micro-waves in wildtype mice and genetic mouse models of disease, particularly if high titre virus loads are used. The aim of this article is not to discredit syn-Ca²⁺ indicators, a tool that we greatly appreciate ourselves, but to sensitize the field to artefactual transduction-induced aberrant Ca²⁺ micro-waves. This article seeks to inform and alert others to carefully evaluate their Ca²⁺ indicator expression approach for in vivo Ca²⁺ imaging of the hippocampus, which is becoming increasingly popular. There is certainly a much greater number of safe alternate hippocampal Ca²⁺ indicator viral expression approaches than has been reported here, and we encourage others to report on viral Ca²⁺ indicator transduction safety profiles. Indeed, others have also encountered these artefactual events as recent social media posts attest (Application Specialist Team 2023), and with more Ca²⁺ indicators becoming available, the open and timely reporting of transduction safety profiles will reduce unnecessary animal experiments, save laboratory resources and time in future investigations into hippocampal function in health and disease.

Materials and Methods

Animals

All experiments followed the institutional guidelines of the Animal Care and Use committee of respective institutions (University of Bonn, DZNE and Columbia university). We used wild-type C57BL/6J mice, Thy1-GCaMP6 mice (C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J; Jackson Lab Stock No: 025776, or C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J; Jackson Lab Stock No: 024276 (Dana et al. 2014)), Vglut1-IRES2-Cre-D mice (Jackson Lab Stock No: 037512) crossed with Ai162(TIT2L-GC6s-ICL-tTA2)-D mice (Jackson Lab Stock No: 031562), PV-Cre::APPswe/PS1dE9 (cross between B6;129P2-Pvalbtm1(cre)Arbr/J, Jackson Lab Stock No: 008069, and B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax, Jackson Lab Stock No: 034832) or Scn2a^A263V mice (from Dirk Isbrandt see Schattling et al. 2016). Mice were kept under a light schedule of 12h on/12h off, constant temperature of 22 ± 2°C, and humidity of 65%. They had ad libitum access to water and standard laboratory food at all time. All efforts were made to minimize animal suffering and to reduce the number of animals used.

Virus injections

For in vivo two-photon imaging experiments, GECIs were virally transduced using injection of an AAV (see Table 1 and Table 2).

Table 2:

At the IEECR/University of Bonn

Mice (∼6 wks of age) received ketoprofen (Gabrilen, Mibe; 5 mg/kg body weight; injection vol. 0.1 ml/10 g b.w., s.c.) for analgesia and anti-inflammatory treatment 30 minutes prior to induction of anesthesia. Then, mice were anesthetized with 2-3% isoflurane in an oxygen/air mixture (25/75%) and then placed in a stereotactic frame. Eyes were covered with eye-ointment (Bepanthen, Bayer) to prevent drying and body temperature was maintained at 37°C using a regulated heating plate (TCAT-2LV, Physitemp) and a rectal thermal probe. After hair removal and superficial disinfection, a drop of 10% lidocaine was used to locally anesthetise the area. After 3-5 mins, a flap of skin was removed about 1 cm² around the middle of the skull. Residual soft tissue was then removed from the skull with a scraper and 3% H2O2/NaCl solution. After complete drying, cranial sutures served as landmarks for the determination of injection sites. For virus injection, a burr hole was carefully drilled through the skull using a dental drill, avoiding excessive heating and injury to the meninges by intermittent cooling with sterile PBS. Coordinates were: for CA1, anterioposterior [AP] measured from bregma 1.9 mm, lateral [L] specified from midline 1.6 mm, dorsoventral [DV] from surface of the skull 1.6 mm; for dentate gyrus, AP 2.4 mm; L 1.6 mm; x3 injections at DV 2.7, 2.5, 2.1 mm. Virus particles (see Table 1) were slowly injected (20-100 nl/min).To prevent reflux of the injected fluid upon cannula retraction, it was left in place until 5 min post-injection, and then carefully lifted.

At the DZNE

A more detailed procedure was described previously (Fuhrmann et al. 2015; Poll et al. 2020). Briefly, the mice (8-43 wk) were anesthetized with i.p. injection of Ketamine (0.13□mg/g) and Xylazine (0.01□mg/g); head-fixed using a head holder (MA-6N, Narishige, Tokyo, Japan) and placed into a motorized stereotactic frame (Luigs-Neumann, Ratingen, Germany). Body temperature was constantly controlled by a self-regulating heating pad (Fine Science Tools, Heidelberg, Germany). After skin incision and removal of the pericranium, position of the injection 34□G cannula was determined in relation to bregma. A 0.5□mm hole was drilled through the skull (Ideal micro drill, World Precision Instruments, Berlin, Germany). Stereotactic coordinates were taken from Franklin and Paxinos, 2008 (The Mouse Brain in Stereotaxic Coordinates, Third Edition, Academic Press). Virus (see Table 1) was injected in two loci with the following CA1 coordinates: AP 1.95 mm; L 1.5 mm; DV 1.15 mm at a speed of 100nL/min

At Columbia University

A more detailed procedure was described previously (Wenzel et al. 2017, 2019a). Briefly, mice (8-20 wks) were anesthetized with isoflurane (initial dose 2–3% partial pressure in air, then reduction to 1–1.5%). For viral injections, a small cranial aperture was established using a dental drill above somatosensory cortex (coordinates from bregma: AP 2.5 mm, L 0.24 mm, DV 0.2 mm), or V1 (coordinates from lambda: AP =2.5 mm, L = 0.02 mm, DV 0.2-0.3 mm), or the hippocampus (coordinates from bregma, CA1: -1.9mm, -1.6mm, - 1.6mm, CA3: -2.2 mm, -2.3 mm, -2.7 mm). A glass capillary pulled to a sharp micropipette was advanced with the stereotaxic instrument, and virus particles (see Table 1) were injected into putative layer 2/3 of neocortex over a 5 min period at 50nl/min, or hippocampus over 12.5 min using a UMP3 microsyringe pump (World Precision Instruments).

At University of Zurich

A more detailed procedure was described previously (Rupprecht et al. 2023). Briefly, mice (5 months old) were anesthetized using isoflurane (5% in O₂ for induction, 1-2% for maintenance during surgery) and provided with analgesia (Metacam 5 mg/kg bodyweight, s.c.). Body temperature was maintained at 35-37°C using a heating pad. An incision was made into the skin after local application of lidocaine. Viral particles (Table 1) were injected into CA1 (coordinates AP -2.0 mm, ML -1.5 mm from Bregma, DV -1.3) using a glass pipette with a manually driven syringe at a rate of approx. 50 nl/min. The injection pipette was left in place for further 5mins before being slowly retracted.

In vivo imaging window implantation procedure

In vivo imaging window implantation procedure: Cranial window surgery was performed to allow imaging from the dorsal hippocampal CA1/CA3 region or neocortex.

At the IEECR, University of Bonn

30 minutes prior to induction of anesthesia, buprenorphine was administered for analgesia (Buprenovet, Bayer; 0.05 mg/kg body weight; injection vol.0.1 ml/20 g b.w., i.p.). Further, dexamethasone (Dexa, Jenapharm; 0.1mg/20g b.w.; injection vol. 0.1 ml/20 g b.w., i.p.), and ketoprofen (Gabrilen, Mibe; 5 mg/kg b.w.; injection vol. 0.1 ml/10 g b.w., s.c.) were applied to counteract inflammation, swelling and pain. Mice were anesthetized with 2-3% isoflurane in an oxygen/air mixture (25/75%) and then placed in a stereotactic frame. Eyes were covered with eye-ointment (Bepanthen, Bayer), body temperature was maintained at 37°C by closed-loop regulation through a warming pad (TCAT-2LV, Physitemp) and a rectal thermal probe. Throughout the course of the surgical procedure, the isoflurane dose was successively reduced to about 1-1.5% at a gas flow of ∼0.5 ml/minute. A circular craniotomy (∼Ø 3 mm) was established above the right hemisphere/hippocampus within the central opening (Ø ∼7mm) of the head plate using a dental drill. Cortical tissue was carefully aspirated until the alveolar fibers above CA1 could be visually identified. A custom-made silicon cone (top Ø 3 mm, bottom Ø 2 mm, depth 1.5 mm, RTV 615, Moimentive) attached to a cover glass (Ø 5 mm, thickness 0.17 mm) was inserted and fixed with dental cement around the edges of the cover glass (see Masala et al. 2022). Postoperatively, all mice received analgetic treatment by administering buprenorphine twice daily (Buprenovet, Bayer; 0.05 mg/kg b.w.; injection vol. 0.1 ml/20 g b.w., i.p) and ketoprofen once daily (Gabrilen, Mibe; 5 mg/kg b.w.; injection vol. 0.1 ml/10 g b.w., s.c.) for 3 consecutive days post-surgery. Throughout this time, animals were carefully monitored twice daily. Animals typically recovered from surgery within 24-48 hours, showing normal activity and no signs of pain or distress.

At the DZNE, Prior to surgery mice were anesthetized with an intraperitoneal injection of ketamine/xylazine (0.13/0.01□mg□per□g of body weight). Additionally, an anti-inflammatory (dexamethasone, 0.2□mg□per□kg) and an analgesic drug (buprenorphine hydrochloride, 0.05□mg□per□kg; Temgesic, Reckitt Benckiser Healthcare) were subcutaneously administered. A cranial window (Ø 3 mm) was implanted above the right hippocampus as previously described (Poll et al. 2020).

At Columbia University

For neocortical imaging, directly following virus injection, the craniotomy was covered with a thin glass cover slip (3×3mm, No. 0, Warner Instruments), which was fixed in place with a slim meniscus of silicon around the edge of the glass cover and finally cemented on the skull using small amounts of dental cement around the edge. For hippocampal imaging, a small area of cortex (around 1.5×1.5mm) above left CA1 was removed by gentle suction down to the external capsule, as described previously (Dombeck et al. 2010; Wenzel et al. 2019b). The site was repeatedly rinsed with sterile saline until no further bleeding could be observed. Then, a small UV-sterilized miniature glass plug (1.5×1.5mm, BK7 glass, obtained from BMV Optical), glued to the center of a thin glass coverslip (3×3mm, No. 0, Warner Instruments) with UV-sensitive glue, was carefully lowered onto the external capsule, and until the edges of the attached glass cover touched the skull surrounding the craniotomy. Finally, the plug was fixed in place with a slim meniscus of silicon around the edge of the glass cover and by applying small amounts of dental cement around the edge of the glass cover.

At University of Zurich

A more detailed procedure was described previously (Rupprecht et al. 2023). Briefly, 2 weeks after virus injection, the hippocampal window was implanted. Two layers of light-curing adhesive (iBond Total Etch, Kulzer) were applied to the exposed skull, followed by a ring of dental cement (Charisma, Kulzer). A 3-mm diameter ring was drilled into the skull, centered at the previous injection site. The cortex in the exposed region was carefully aspirated using a vacuum pump until the stripes of the corpus callosum became visible. The corpus callosum was left intact. A cylindrical metal cannula (diameter 3 mm, height 1.2-1.3 mm) attached with dental cement to a coverslip (diameter 3 mm) was carefully inserted into the cavity. The hippocampal window was fixed in place using UV-curable dental cement (Tetric EvoFlow, Ivoclar).

Two-photon Ca²⁺ imaging

A variety of standard commercially available two-photon systems were used at the different institutes to record the circular Ca²⁺ waves.

At the IEECR, University of Bonn

a commercially available tw-photon microscope (A1 MP, Nikon) equipped with a 16x water-immersion objective (N.A.=0.8, WD=3 mm, CFI75 LWD 16X W, Nikon) controlled by NIS-Elements software (Nikon). GCaMP6s was excited at 940 nm using a Ti:Sapphire laser system (∼60 fs laser pulse width; Chameleon Vision-S, Coherent). Emitted photons were collected using gated GaAsP photomultipliers (H11706-40, Hamamatsu). Several individual tif series were recorded by resonant scanning at a frame rate of 15 Hz for a total 20-40 minutes per imaging session.

At the DZNE

Recordings of Ca²⁺-changes were performed with a galvo-resonant scanner (Thorlabs, Newton, USA) on a two-photon microscope equipped with a 16x water immersion objective with a numerical aperture of 0.8 (N16XLWD-PF, Nikon, Düsseldorf, Germany) and a titanium sapphire (Ti:Sa) 80 MHz Cameleon Ultra II two-photon laser (Coherent, Dieburg, Germany) that was tuned to 920 nm for GCaMP6m fluorescence excitation. GCaMP6m fluorescence emission was detected using a band-pass filter (525/50, AHF, Tübingen, Germany) and a GaAsP PMT (Thorlabs, Newton, USA). ThorImageLS software (Thorlabs, version 2.1) was used to control image acquisition. Image series (896 x 480 pixels, 0.715 µm/pixel, or 640 x 256 pixels) were acquired at 30.3 Hz or 32.3 Hz.

At Columbia University

Neural population activity was recorded using a commercially available two-photon microscope (Bruker; Billerica, MA) and a Ti:Sapphire laser (Chameleon Ultra II; Coherent) at 940 nm through a 25x objective (Olympus, water immersion, N.A. 1.05). Resonant galvanometer scanning and image acquisition (frame rate 30.206 fps, 512 x 512pixels) were controlled by Prairie View Imaging software.

At University of Zurich

Neuronal population activity was recorded using a custom-built two-photon microscope (see Rupprecht et al. 2023). Briefly light from a femtosecond-pulsed laser (MaiTai, Spectra physics; tuned to 960 nm; power below objective 40-45 mW) was used to scan the sample below a 16x objective (Nikon, water immersion, NA 0.8). Image acquisition and scanning (frame rate 30.88Hz, 622×512 pixels) were controlled by custom-written software (Chen et al. 2016).

Analysis of aberrant Ca²⁺ micro-waves

To remove motion artifacts, recorded movies were registered using a Lucas–Kanade model (Greenberg & Kerr 2009), or the ImageJ plugin moco (available through the Yuste web page, or https://github.com/NTCColumbia/moco) (Dubbs et al. 2016) or in the case of R-CaMP the NoRMCorre algorithm (Pnevmatikakis & Giovannucci 2017).

We determined the diameter of the calcium waves in a semi-automated fashion from the raw tif-series. Using ImageJ software, we first drew an orthogonal line across the largest aspect of each calcium wave progressing through the field of view, which resulted in a fluorescent histogram for each wave. Using custom code (MATLAB R2020b), we further analysed all histograms for each mouse and imaging timepoint. First, we applied a gentle smoothing, max-normalized each histogram, and max-aligned all histograms of a given imaging session. Then, after excluding the 10% lowest fluorescent values, the width of each calcium wave and a mean value was calculated for each time point/imaging session. Finally, the resulting pixel values were converted to micrometer based on the respective objective (@ 512×512p×l and 1x Zoom: Nikon 16x, NA 0.8, 3mm WD: 1.579 µm/pxl; Olympus 25x, NA 1.05 2mm WD: 0.92 µm/pxl). The speed of the Ca²⁺ micro-waves was calculated from the duration and path length of the events visually identified and manually tracked in the FOV.

Histochemistry

To verify successful viral transduction and window position, animals were deeply anesthetized with ketamine (80 mg/kg body weight) and xylazine (15 mg/kg body weight). After confirming a sufficient depth of anesthesia, mice were heart-perfused with cold phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in PBS. Animals were decapitated and the brain removed and stored in 4% PFA in PBS solution for 24 hours. Fifty to 100 µm thick coronal slices of the hippocampus were cut on a vibratome (Leica). For nuclear staining, brain slices were kept for 10 min in a 1:1000 DAPI solution at room temperature. Brain slices were mounted and the green and blue channel successively imaged under an epi fluorescence or spinning disc microscope (Visitron VisiScope).

Acknowledgements

We sincerely thank Lea Adenauer, Laura Kück and Olga Zabashta for excellent technical support with animal husbandry and immunohistochemistry. We acknowledge the support of the Imaging Core Facility of the Bonn Technology Campus Life Sciences funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 388169927.

Funding

The work was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) with a Research Group FOR-2715 to T.K. and H.B, and SFB1089 to M.F, M.W. and H.B. The work was further supported by the BONFOR Program UoB (M.W.: #2019-2- 04), the Hertie Network of Excellence in Clinical Neuroscience (M.W.: #P1200008), European Research Council (M.W.: StG #101039945; F.H.: AdvG #670757) and the Swiss National Science Foundation (project grant 310030B_170269 and Sinergia grant CRSII5 180316 to F.H.; Ambizione grant PZ00P3_209114 to P.R.) Additional support was provided by the National Science Foundation (NSF GFRP to D.A.O; 2203119 to R.Y.), National Institute of Neurological Disorders and Stroke (F99NS134209 to D.A.O. and RM1NS132981) and National Institute of Mental Health (R01MH115900 to R.Y.), NSF (2203119) to R.Y. and a Vannevar Bush Faculty Fellowship (ONR N000142012828 to R.Y.)

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Author Contributions

M.W. and T.K. conceived and planned the study. N.M., M.W. M.M, E.A., D.A.O, F.D., P.R performed experiments. F.H., R.Y., M.F., H.B. provided infrastructure and experimental expertise. M.W. and T.K. analysed data and wrote the manuscript with input from all authors.

Conflict of Interest

None of the authors have a conflict of interest.