Genetic and protein-protein interaction studies show evidence of DSB protein sub-complexes in C. elegans.
A) Summary of the genetic interaction results. The complete set of results is given in Figure S3. B) Genetic groups are based on data presented here and previously published (Chung et al., 2015; Mateo et al., 2016; McClendon et al., 2016; Janisiw et al., 2020). C) Summary of the yeast two-hybrid assay results. The complete set of results is given in Figure S1A. D) Schematic representation of the interaction network based on Y2H interactions. Thick line: Strong interaction between two proteins. Thin line: weak interaction between two proteins. E) Different DSB-1 constructs (detailed in F) were used to analyze yeast-two-hybrid interactions with SPO-11, DSB-2, and DSB-3. F) Scheme representation of the wild-type DSB-1 structure, and the different truncations used in E) For more details about the DSB-1 sequence, protein structure, and deletions used in these experiments, see Figure S5.