asm-3 genetically functions together with PC synthesis pathway to regulate fat content in DA-fed worms and is transcriptionally upregulated through B12-SAM-PC axis
(A) O.R.O staining results of wild-type, asm-3(ok1744) mutants and asm-3(ok1744); tpEx941 mutants, which contains overexpressed asm-3 driven by asm-3 promoter. (B) O.R.O staining results of wild-type, pcyt-1(et9), asm-3(ok1744), and asm-3(ok1744); pcyt-1(et9) mutants. (C) Images and quantitative results of Pasm-3::GFP signals in worms under OP, DA, or OP with B12 supplementation diet. (D) Images and quantitative results of the signals of asm-3 transcriptional reporter in wild-type, sams-1(ok3033), pcyt-1(et9), and asm-3(ok1744) mutant background. Statistical analysis was performed using Two-tailed T tests and the data is presented as mean ± SEM. # indicates comparison of dietary effects between OP- and DA-fed worms with the same genotype. * indicates comparison between different strains feeding the same diet. * / # P < 0.05, ** / ## P < 0.01, *** / ### P < 0.001, **** / #### P < 0.0001.