Dconfocal values calculated from individual FRAP and merged FRAP curves.
FRAP curves and Dconfocal values for Fluorescein (A-D), GSK3 inhibitor (E-H) and Quinacrine (I-L) inside HeLa cell cytoplasm are shown. Three representative individual FRAP profiles with exponential fits with R= 0.80-0.90 for each of the compounds respectively are shown in (A), (E), and (I). Individual Dconfocal values and their geometric means from individual FRAP curves are shown for Fluorescein((B), GSK3 inhibitor (F) and Quinacrine (J). (C), (G) and (K) show the merged FRAP curves over 30 independent measurements. This provides an improved fit (R=0.99). Panels (D), (H) and (L) show Dconfocal values calculated from the merged FRAP profiles. Error bars represent SE calculated from fitting the FRAP progression curves.