Energetic drive limits sleep rhythm development

(A-D) Feeding rate (# of mouth hook contractions per 5 min) of L2 controls (A), L3 raised on regular (ctrl) food (B), L3 clock mutants (C), and L3 raised on low sugar (L.S.) food (D) across the day. (E) Sleep duration at CT1 and CT13 in L3 raised on regular (ctrl) and L.S. food. (F) Arousal threshold at CT1 and CT13 in L3 raised on regular (ctrl) and L.S. food. A-D, n=18-20 larvae; E, n=29-34 larvae; F, n= n=100-172 sleep episodes, 18 larvae per genotype. One-way ANOVAs followed by Sidak’s multiple comparisons tests [(A-D)]; Two-way ANOVAs followed by Sidak’s multiple comparison test [(E-F)]. For this and all other figures unless otherwise specified, data are presented as mean ± SEM; n.s., not significant, *P<0.05, **P<0.01, ***P<0.001.

Immature feeding strategies limit LTM.

(A) Feeding rate of L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 30°C at CT13. (B-D) Sleep duration (B), bout number (C), and bout length (D) of L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 30°C. (E-G) Arousal threshold (E), long-term aversive memory performance (F), and short-term aversive memory performance (G) in L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 30°C. (H,I) Short-and long-term term aversive memory performance in L3 raised on ctrl and L.S. food. A, n=18-20 larvae; B-D, n=24-61 larvae; E, n=125-160 sleep episodes, 18 larvae per genotype; F-I, n=8 PIs (240 larvae) per genotype. One-way ANOVAs followed by Sidak’s multiple comparisons tests [(A) and (E-G)]; Two-way ANOVAs followed by Sidak’s multiple comparison test [(B-D)]; unpaired two-tailed Student’s t-test [(H) and (I)].

Deeper sleep in L2 is energetically disadvantageous.

(A, B) Sleep duration (A) and arousal threshold (B) of L2 control fed vehicle control (L2) or Gaboxadol (L2 Gab). (C, D) Total body weight of L2 (C) (in groups of 10) or L3 (D) (in groups of 5) fed vehicle control or Gaboxadol (Gab). (E) Long-term aversive memory performance in L2 fed vehicle control (L2) or Gaboxadol (L2 Gab). (F, G) Sleep duration (F) and arousal threshold (G) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. (H) Long-term aversive memory performance of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. (I, J) Total body weight (I) and total body length (J) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. A, n=28 larvae; B, n=110-220 sleep episodes, 18 larvae per genotype; C, n=5-7 groups (50-70 larvae); D, n=8 groups (40 larvae); E, n=8 PIs (240 larvae) per genotype; F, n=33-36 larvae; G, n=234-404 sleep episodes, 30-40 larvae per genotype; H, n=8 PIs (240 larvae) per genotype; I, n=5 groups (25 larvae); J, n=31-32 larvae. Unpaired two-tailed Student’s t-tests [(A-E)]; one-way ANOVAs followed by Sidak’s multiple comparisons tests [(F-J)].

DN1a-Dh44 circuit formation is developmentally plastic.

(A, C) GCaMP6 signal in Dh44 neurons with activation of DN1a neurons in L3 controls (A) and L3 raised on L.S. food (C). Red bar indicates ATP application and gray bar indicates AHL application. (B, D) Maximum GCaMP change (ΔF/F) for individual cells in L3 controls (B) and L3 raised on L.S. food (D). (E-G) GCaMP7 signal in Dh44 neurons during bath application of 1 µM CCHa1 synthetic peptide in L3 controls (E), L2 controls (F) and L3 raised on L.S. food (G) brains. Red/blue bar indicates timing of CCHa1 (blue) or buffer (AHL, red) application and gray bar indicates timing of washout AHL application. A-D, n=12-18 cells, 8-10 brains; E-G, n=11-15 cells, 5-10 brains. Unpaired two-tailed Student’s t-tests [(B) and (D)]; Mann-Whitney U test [(E-G)].

Dh44 neurons require glucose metabolic genes to regulate sleep-wake rhythms.

(A-C) Sleep duration in L3 expressing UAS-Glut1-RNAi (A), UAS-Hex-C-RNAi (B), and UAS-Pyk-RNAi (C) with Dh44-Gal4 and genetic controls at CT1 and CT13. (D) Feeding rate (# of mouth hook contractions per 5 min) of L3 expressing UAS-Hex-C-RNAi with Dh44-Gal4 and genetic controls at CT13. (E-G) Sleep duration in L2 expressing UAS-Glut1-RNAi (E), UAS-Hex-C-RNAi (F), and UAS-Pyk-RNAi (G) with Dh44-Gal4 and genetic controls at CT1 and CT13. (H, I) Sleep duration in L3 expressing UAS-GCN2-RNAi (H) and UAS-crc-RNAi (I) with Dh44-Gal4 and genetic controls at CT1 and CT13. A-C, n=32-40 larvae; D, n=20 larvae; E-I, n=32-40 larvae. Two-way ANOVAs followed by Sidak’s multiple comparison test [(A-C) and (E-I)]; One-way ANOVAs followed by Sidak’s multiple comparisons tests [(D)].

Larvae reared on low sugar diet develop normally.

(A) Feeding amount of L2 controls, L3 raised on regular (ctrl) food, and L3 raised on low sugar (L.S.) food at CT1 and CT13. (B, C) Sleep bout number (B) and bout length (C) at CT1 and CT13 in L3 raised on regular (ctrl) and L.S. food. (D) Total body weight of early L3 (in groups of 5) raised on ctrl or L.S. food. (E) Total body length of early L3 raised on ctrl or L.S. food. (F) Developmental analysis of time to pupal formation of animals raised on ctrl or L.S. food. (G-I) Naïve OCT, AM, and quinine preference in L3 raised on ctrl and L.S. food. B-C, n=29-34 larvae; D, n=30 larvae per food condition; E, n=33-40 larvae; F, n=100-170 larvae; G-I, n=6 PREFs (180 larvae) per genotype. Two-way ANOVAs followed by Sidak’s multiple comparison test [(B-C)]; Unpaired two-tailed Student’s t-tests [(D-E) and (G-I)].

Baseline odor preferences, feeding, and sleep are not affected by npf-Gal4 manipulations.

(A) Feeding rate of L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 22°C at CT13. (B-E) Sleep duration (B), bout number (C), bout length (D), and arousal threshold (E) in L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at CT1 and CT13 at 22°C (temperature controls). (F) Long-term aversive memory performance in L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 22°C (temperature controls). (G-I) Naïve OCT, AM, and quinine preference in L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 30°C. A, n=18-20 larvae; B-D, n=22-27 larvae; E, n=120-205 sleep episodes, 18 larvae per genotype; F, n=8 PIs (240 larvae) per genotype; G-I, n=6 PREFs (180 larvae) per genotype. One-way ANOVAs followed by Sidak’s multiple comparisons tests [(A), (E), and (F-I)]; Two-way ANOVAs followed by Sidak’s multiple comparison test [(B-D)].

Baseline sleep and odor preferences are not disrupted by R76G11-Gal4 manipulations.

(A, B) Sleep bout number (A) and bout length (B) of L2 control fed vehicle control (L2) or Gaboxadol (L2 Gab). (C, D) Sleep bout number (C) and bout length (D) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. (E-H) Sleep duration (E), bout number (F), bout length (G), and arousal threshold (H) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 22°C (temperature controls). (I-K) Naïve OCT, AM, and quinine preference in L2 expressing R76G11- Gal4>UAS-TrpA1 and genetic controls at 30°C. (L) Short-term aversive memory performance of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. A, B, n=28 larvae; C-D, n=33-36 larvae; E-G, n=27-29 larvae; H, n=145-316 sleep episodes, 30-40 larvae per genotype; I-K, n=6 PREFs (180 larvae) per genotype; L, n=8 PIs (240 larvae) per genotype. Unpaired two-tailed Student’s t-tests [(A-B)]; One-way ANOVAs followed by Sidak’s multiple comparisons tests [(C-L)].

Glucose metabolic gene manipulations affect L3 sleep.

(A, B) Sleep bout number (A) and bout length (B) in L3 expressing UAS-Glut1-RNAi with Dh44-Gal4 and genetic controls at CT1 and CT13. (C, D) Sleep bout number (C) and bout length (D) in L3 expressing UAS-Hex-C-RNAi with Dh44-Gal4 and genetic controls at CT1 and CT13. (E, F) Sleep bout number (E) and bout length (F) in L2 expressing UAS-Glut1-RNAi with Dh44-Gal4 and genetic controls at CT1 and CT13. (G, H) Sleep bout number (G) and bout length (H) in L2 expressing UAS-Hex-C-RNAi with Dh44-Gal4 and genetic controls at CT1 and CT13. (I, J) Sleep bout number (I) and bout length (J) in L3 expressing UAS-GCN2-RNAi with Dh44-Gal4 and genetic controls at CT1 and CT13. A-J, n=32-40 larvae. Two-way ANOVAs followed by Sidak’s multiple comparison test [(A-J)].