Abstract
Summary
TNF has a dual effect in Plasmodium infection, bolstering the host’s immune defense while also triggering disease. Here, we show that TNF signaling hampers physical activity, food intake, and energy expenditure while enhancing glucose uptake by the liver and spleen as well as controlling parasitemia in P. chabaudi (Pc)-infected mice. We also demonstrate that TNF is required for expression of inducible nitric oxide synthase (iNOS), stabilization of HIF-1α, expression of glucose transporter GLUT1 and enhanced glycolysis in monocytic cells from Pc-infected mice. Importantly, Pc- infected iNOS-/-, TNFRΔLyz2 and HIF-1αΔLyz2 mice show impaired release of TNF and glycolysis in monocytes, together with increased parasitemia and disease tolerance. Together, our findings reveal that TNF-iNOS-HIF-1α-induced glycolysis in monocytes plays a critical role in host defense and sickness behavior in Pc-infected mice.
Introduction
Malaria caused by parasites from the Plasmodium genus is the most common mosquito-borne infectious disease (1). In 2021, approximately 247 million cases were reported resulting in nearly 619 000 deaths worldwide (2). The clinical symptoms include periodic fever episodes that occur synchronously with the rupture of infected red blood cells and the release of erythrocyte and parasite debris (3). Besides the characteristic development of fever, which is caused by systemic pro- inflammatory cytokine release (3), metabolic disorders like hypoglycemia and hyperlactatemia also occur in patients with malaria. High lactate plasma levels are an important prognostic factor in patients with severe malaria and are related to the development of acidosis (4–9).
Successful immunity to Plasmodium infection requires the participation of monocytic cells, which have a central role in sensing and phagocytosing parasitized (10). In experimental models, monocytes are the major cells responsible for secreting pro-inflammatory pyrogenic cytokines, such as IFN-γ, IL-1β and TNF, during acute malaria episodes (11–13). Among these pro-inflammatory cytokines, TNF plays an important role in the pathophysiology of malaria (14). In other contexts, TNF has been identified as a major inducer of glucose uptake and metabolism in host immune cells (14–17). Glucose is metabolized inside cells through glycolysis to generate energy and biosynthetic intermediates for cell growth, activation and proliferation (18, 19). Moreover, the numerous intermediate metabolites that participate in the glycolysis-mediated conversion of glucose into pyruvate also play important roles in maintaining the activity and effector functions of innate immune cells (20).
The hypoxia-inducible factor 1 alpha (HIF-1α) is one of the central regulators of inflammation and glucose metabolism in myeloid cells (21). Under normoxic conditions, HIF-1α is hydroxylated and degraded by the proteasome. Under hypoxic conditions resulting from high levels of reactive nitrogen intermediates (RNI), altered mitochondria function and release of reactive oxygen species (ROS), the activity of prolyl-hydroxylase domain (PHD) enzymes is inhibited, leading to stabilization of HIF-1α, and its subsequent translocation to the nucleus (22, 23). In addition, the expression of HIF-1α is upregulated in response to TNF. This increase in HIF-1 levels when occurring in macrophages has been shown to be associated with enhanced resistance to infection in different experimental models (21, 24–28).
Nevertheless, it is unclear how glucose metabolism, HIF-1α and their interplay with TNF affects innate immune cells and host resistance to Plasmodium parasites. Here, we investigated the involvement of TNF, RNI and HIF-1α in regulating glucose metabolism in monocytic cells and whether this pathway plays a role in is host resistance and disease tolerance in experimental murine malaria. Our findings demonstrate that both TNF and inducible nitric oxide synthase (iNOS) promote the expression and stabilization of HIF-1α that in turn enhances expression of the glucose transporter GLUT1 and glycolysis in monocytic cells in P. chabaudi- (Pc-) infected mice. Importantly we show that this biochemical shift in monocytes has an important role in regulating host energy metabolism, resistance to infection and disease outcome in experimental malaria.
Results
TNF signaling regulates disease symptoms and energy expenditure in P. chabaudi-infected mice
TNF is involved in the development of protective immunity and clinical manifestations of malaria in both humans and experimental models (29–31). We first analyzed the time course of parasitemia in Pc-infected C57BL/6 mice and measured blood glucose levels daily at the end of the dark cycle. Parasites were detected in the circulation, starting at 3 days post-infection (dpi), and the peak of parasitemia was observed at 8 dpi (Figure 1A). Blood glucose levels significantly decreased at 8 dpi, coinciding with the peak of parasitemia. At later time points, parasitemia decreased, and glycaemia returned to homeostatic levels (Figure 1B). As previously demonstrated (32), circulating levels of TNF and the expression of TNF mRNA in the liver peaked at 6 am (end of dark cycle) at 8 dpi (Figure 1C and 1D). We also observed that the percentage of iRBCs was higher in TNFR-/- (TNFR p55p75-/-) compared to C57BL/6 mice (Figure 1E), as previously reported (31). In addition, while infected C57BL/6 mice displayed hypothermia in response to infection, the rectal temperature of infected and uninfected TNFR-/- mice was similar (Figure 1F). Furthermore, circulating levels of MCP1, TNF and IFN-γ were significantly lower in TNFR1-/- (TNFR p55-/-) compared to C57BL/6 mice (Figures 1G- I), whereas no difference was observed in IL-10 levels (Figure 1J). These results support the concept that TNF signaling promotes the systemic release of pro- inflammatory cytokine and plays a key role in controlling parasitemia, along with disease symptoms in Pc-infected mice.
To further address if TNF signaling in Pc-infected mice promote malaria disease parameters, we evaluated alterations in host energy metabolism, as indicated in physical activity, food intake, overall energy expenditure and respiratory exchange rate in naïve or Pc-infected C57BL/6 and TNFR-/- mice during a 24h period, at the 8 dpi. We observed that in naïve animals, all of these parameters were similar in TNFR-/- and C57BL/6 mice (Figures 2A-D, top panels and Figures 2E-H). In contrast, all the evaluated parameters were decreased in infected C57BL/6 mice compared to their naïve counterparts during the light and dark cycles. When we analyzed only infected mice, the alterations in all parameters were milder in TNFR-/- compared to C57BL/6 mice (Figures 2A-D bottom panels and 2E-H).
We then asked whether Pc infection might also affect body fat mass and tissue glucose uptake. We observed a major depletion of fat mass in infected C57BL/6 mice at 9 dpi, which was less pronounced in TNFR-/- animals (Figure 2I), while the percentage of lean mass was not altered in either wild type or TNFR-/- mice (Figure 2J). We also found that Pc infection resulted in a marked increase in glucose uptake in the liver and spleen of C57BL/6 mice but not in TNFR-/- animals (Figure 2K). In contrast to the spleen and liver, glucose uptake in skeletal muscle or white and brown adipose tissue remained unaltered following Pc infection in C57BL/6 mice (Supplemental 1A-C). The increase in glucose uptake was associated with the development of hypoglycemia in C57BL/6 but not in TNFR-/- mice infected with Pc (Figure 2L).Together, these results support a role for TNF in regulating glucose and energy metabolism in Pc-infected mice, two responses which play an important role in the pathophysiology of experimental malaria.
Hepatic non-parenchymal cells display enhanced GLUT1 receptor expression and shift to glycolytic metabolism following P. chabaudi infection
The liver has a key role in regulating host glucose metabolism (33). Therefore, we asked whether Pc infection alters the expression of genes related to carbohydrate metabolism in the liver as measured by RNA-SEQ. We observed an overall enhancement in the expression of genes related to glycolysis and a reduction in the expression of genes associated with the Tricarboxylic (TCA) cycle and gluconeogenesis at 8 dpi (Figure 3A). Enhanced glycolysis relies on the heightened glucose uptake by cells mediated by different glucose carriers (34, 35). However, the expression of GLUT2 (Slc2a2), GLUT5 (Slc2a5) and GLUT9 (Slc2a9) was decreased, whereas of GLUT1 (Slc2a1), GLUT3 (Slc2a3) and GLUT6 (Slc2a6) was increased in the liver of C57BL/6 infected as compared with naïve mice (Figure 3A). Among the transporters whose expression was increased, GLUT3 is expressed mainly in neurons (36), while Slc2a6 GLUT6 has been shown to be located in lysosomes and does not mediate glucose uptake (37). GLUT1, on the other hand, is a ubiquitously expressed and highly effective transporter of glucose. Also, GLUT1 is the most well-characterized surface glucose transporter in immune cells (18, 38, 39). Therefore, we next quantified GLUT1 protein, and, as expected, an enhancement in GLUT1 levels was found in the liver of Pc-infected C57BL/6 mice, as compared to the uninfected controls (Figure 3B).
Hepatocytes have an important role in glucose uptake from the circulation, and they do this primarily through GLUT2 (38), whose mRNA expression was downregulated (Figure 3A) and protein expression unchanged in response to Pc infection (Figure 4K). Since GLUT1 expression can also be induced in hepatocytes (18), we evaluated if GLUT1 expression was enhanced in hepatocytes and non- parenchymal cells from the liver of infected mice. We found that at 8 dpi, GLUT1 expression was not altered in hepatocytes (Figure 3C) but increased in non- parenchymal cells (Figure 3D). We also assessed the metabolic activity of these same hepatic cell populations by quantifying their extracellular acidification rate (ECAR) and oxygen consumption rate (OCR), which are indicatives of how much glucose is being metabolized through glycolysis and the TCA cycle, respectively. Consistent with the observed unaltered GLUT1 expression, we found no change in the metabolic profile of hepatocytes (Figure 3E) but instead an increase in ECAR of non-parenchymal cells (Figure 3F) from the liver of Pc-infected mice.
CD11b+ cells from the spleen shift towards glycolytic metabolism in response to P. chabaudi infection
The spleen is the main tissue involved in immunity to Plasmodium infection, where the response of immune cells to infection results in a remarkable splenomegaly (40). Consistent with this, we found that the magnitude of glucose uptake in spleens was higher than in the livers of infected mice (Figure 2K). Therefore based on the data presented above, we next asked whether the increased tissue glucose uptake in experimental malaria was associated with the enhancement of GLUT1 expression and glycolysis in different subsets of immune cells. The levels of GLUT1 in spleens from Pc-infected C57BL/6 mice were also increased at 8 dpi (Figure 3G). We also assessed GLUT1 expression in CD4+ and CD8+ T lymphocytes, B lymphocytes and CD11b+F4/80+/CD11c+/Ly6g- myeloid cells by flow cytometry (gated as shown in Supplemental 3A and 3B). We found that the CD11b+ subset and, to a lesser extent, CD4+ T cells, but not CD8+ T and B lymphocytes, displayed increased expression of GLUT1 in response to infection (Figures 3G, 3H and Supplemental 3C). Importantly, the elevation in GLUT1 expression in monocytic cells was ∼410% versus only ∼29% in CD4+ T cells (Figure 3H). In accordance with the expression of GLUT1 in those cell populations, the metabolic profiles of T and B lymphocytes were not altered in Pc infected mice (Figure 3I). In contrast, splenic CD11b+ cells displayed enhanced glycolysis in infected as compared to naive mice (Figure 3J).
We have previously demonstrated that infection with P. berghei ANKA induces a marked increase in monocyte-derived dendritic cells (MO-DCs) in the spleens of mice (41, 42). Therefore we next analyzed changes in numbers splenic CD11b+F4/80+ cells (which are ≥90% CD11c+Ly6G-) (Supplemental 2), and selected monocytes (G1: DC-SIGN-MHCII-), inflammatory monocytes (G2: DC-SIGN+MHCII-) and MO-DCs (G3: DC-SIGN+MHCII+) in pc infected mice (Figure 3L). As shown in Figure 3K and 3L, Pc infection resulted in increased frequencies of MO-DCs, and reduced percentages of inflammatory monocytes and monocytes in spleens (Figure 3K and 3L). At 8 dpi, expression of GLUT-1 was increased in inflammatory monocytes and MO-DCs but not in monocytes (Figure 3 M). Moreover, when we compared cells from infected mice, the levels of GLUT1 were similar between MO- DCs and inflammatory monocytes but significantly higher in MO-DCs compared to monocytes (Figure 3M). Together these findings suggested that activated monocytes are the primary cells responsible for increased glucose uptake in the spleens in Pc-infected mice.
TNF signaling induces glycolysis in tissues from P. chabaudi-infected mice
As shown above, TNF signaling promotes glucose uptake in livers and spleens from Pc-infected mice (Figure 2K). We next asked whether TNF modulates glucose metabolism in cells from these same organs. We observed that except for Hexokinase-3, the expression of mRNAs of glycolytic enzymes (Hexokinase-1, PFKP and PKM) was increased in C57BL/6 but not TNFR-/- 8-dpi (Figure 4B, D and E), whereas the signature of gluconeogenesis enzymes (G6PC, G6PC3, FBP1, PCK1 and PCK2) mRNA remained unchanged (Figures 4F-J). The expression of GLUT2, the main glucose transporter of hepatocytes, was similar in naïve and infected mice, both in C57BL/6 and TNFR-/- mice (Figure 4K). The expression of GLUT1 protein mirrored the mRNA expression of glycolytic enzymes (Figure 4K and L), and the increased expression of GLUT1 was also lower in splenic CD11b+ cells from Pc-infected TNFR-/- mice (Figure 4L). In agreement with the reduced levels of GLUT1, splenic CD11b+ cells from TNFR-/- infected mice displayed reduced ECAR when compared with CD11b+ from infected wild-type animals (Figure 4M), as evidenced by lower basal and compensatory glycolysis (Figure 4N).
To further confirm that the observed effects were due to TNF signaling in myeloid cells we generated mice with conditional deletion of TNF receptor 1 in lysozyme M- expressing cells (TNFR1ΔLyz2). As expected, we observed a higher parasitemia in TNFR1ΔLyz2 mice than in WT animals (Figure 4O). Moreover, TNFR1ΔLyz2 infected mice did not exhibit decreased rectal temperature (Figure 4P) or blood glucose levels (Figure 4Q). These data demonstrate that during experimental malaria, TNF signaling plays a key role in inducing GLUT1 expression and glucose metabolism in activated monocytes.
TNF and HIF-1α signaling crosstalk regulates glycolytic metabolism in myeloid cells from P. chabaudi-infected mice
The hypoxia-inducible factor 1 alpha (HIF-1α) is an oxygen-regulated transcriptional activator that plays essential roles in mammalian development, metabolism and the pathogenesis of several diseases (43). Its functions are primarily associated with the reprogramming of cellular energetic metabolism in response to hypoxic conditions, while in innate immune cells, HIF-1α can also promote glycolysis and quickly generate ATP and intermediate metabolites that fuel the activation and release of pro-inflammatory cytokines (43). For this reason, we next asked if HIF-1α is involved in the TNF-induced reprogramming of glucose metabolism in immune cells during Pc infection. We observed a higher nuclear expression of HIF-1α in the spleen and liver of infected mice; however, the levels of HIF-1α were lower in livers of TNFR-/- compared to C57BL/6 infected mice (Figure 5A-B).
We next assessed the importance of the HIF-1α pathway for host resistance to malaria by using mice with conditional deletion of HIF-1α in myeloid cells (HIF- 1aΔLyz2) and their WT counterparts (HIF-1afl/fl). It has been previously described that HIF-1α activity can induce the expression of GLUT1 in different mammalian cell populations (20). We found that GLUT1 protein and glycolysis (ECAR) was impaired, respectively, in monocytic cells, and splenic CD11b+ cells from infected, as compared to uninfected HIF-1aΔLyz2 mice (Figures 5C-5E). Importantly, we observed a higher parasitemia in HIF-1aΔLyz2 mice than in WT animals (Figure 5F and Supplemental 3). At 8dpi with Pc, the circulating levels of TNF were decreased in HIF-1aΔLyz2 mice compared to WT animals (Figure 5G). In addition, Pc-infected HIF- 1afl/fl mice splenocytes displayed enhanced TNF secretion following LPS stimulation compared to cells from HIF-1aΔLyz2 infected animals (Figure 5H). Together, these data demonstrate that the induction of the HIF-1a in myeloid cells is important for inducing glycolysis, TNF production and control of Pc infection.
iNOS expression promotes HIF-1α stability, glycolytic metabolism and modulates signs of disease in P. chabaudi-infected mice
Our next step was to understand how TNF promotes HIF-1α expression. It is known that reactive nitrogen intermediates (RNI) induces HIF-1α expression in particular by enhancing the stability of this transcription factor through a mechanism that inhibits its degradation (44). TNF is also widely described as a major inducer of iNOS expression and RNI release by myeloid cells, such as monocytes (45). As expected, we observed that splenocytes from Pc-infected TNFR-/- mice stimulated ex vivo with LPS released lower levels of RNI than LPS-stimulated cells from C57BL/6-infected mice (Figure 6A). Importantly, iNOS-deficient mice presented higher parasitemia than C57BL/6 animals at 8dpi with Pc (Figure 6B), as previously described (46). Similar to what was observed in TNFR-/- animals, infected iNOS-/- mice did show hypothermia at 8dpi with Pc (Figure 6C). Additionally, at this same time point, we did not observe hypoglycemia in the iNOS-/- mice infected with Pc (Figure 6D). As expected, splenic CD11b+ cells from iNOS-/- infected mice displayed increased OCR when compared to C57BL/6 infected with Pc (Figure 6E), indicating increased basal and maximal mitochondrial respiratory capacities in the absence of iNOS (Figure 6F).
In accordance with the results obtained in Pc-infected TNFR-/- mice, iNOS deficiency resulted in reduced hepatic expression of the glycolytic enzymes HK1, HK3, PFKP and PKM (Figures 6 G-J) as well as reduced GLUT1 expression in splenic monocytes (Figure 6K). Consistent with these observations, glycolytic metabolism in splenic monocytic cells of iNOS-/- infected animals was decreased compared to those of C57BL/6 infected mice, as denoted by a lower ECAR (Figure 6L). Taken together, these data demonstrated that RNI induces HIF-1α expression, glycolysis, and TNF release by monocytic cells, leading to control of parasitemia but also promoting clinical signs of disease, such as hypothermia and hypoglycemia, in Pc-infected mice.
Discussion
Malaria disease manifestations include severe changes in host metabolism. During the 1980s, studies reported that decreased glycaemia and increased lactate plasma levels followed by acidosis are common manifestations in patients with severe malaria (5, 47–49). However, more than 40 years later, the mechanisms that lead to hypoglycemia and increased lactate levels during malaria are poorly understood. A better understanding of the mechanisms that mediate host metabolic alterations and the connections of such events with the immune response against the parasite may provide new insights for therapeutic interventions in malaria patients. In this study, we found that TNF signaling mediates changes in host energy metabolism, accompanied by increased expression of GLUT1 and enhanced glycolysis in monocytes. In addition, we found that TNF-induced RNI stabilizes the transcription factor HIF-1α, which promotes both a metabolic shift towards glycolysis and the expression of pro-inflammatory cytokines by monocytes. Furthermore, we observed that TNF, iNOS and HIF-1α have important roles in controlling Pc infection and signs of systemic inflammation in this mouse malaria model.
Interestingly, we found that glucose uptake was significantly increased in spleens and livers of Pc-infected mice and that this effect was partially dependent on TNF signaling. Consistently, TNF promoted increased expression of GLUT1 and glycolytic energy metabolism in non-parenchymal cells and monocytic cells, but not hepatocytes or lymphocytes in livers and spleens, respectively. These results indicate that the hypoglycemia of Pc-infected mice is caused by TNF-driven GLUT1- mediated enhanced glucose uptake and glycolysis in monocytic cells.
Following the rupture of parasitized red blood cells, there is a release of pathogen and danger-associated molecular patterns that activate Toll-like receptors (TLRs), Nod-like receptors (NLRs), and the cyclic GMP–AMP (cGAS) synthase (10, 52). Activation of these innate immune receptors and sensors triggers the production of high levels of pro-inflammatory cytokines, such as TNF, IL-6, IL1β and IL-12, all of which participate in host immunity against Plasmodium infection (53). The importance of the glycolytic pathway for innate immune cell activation has been demonstrated in different studies (20, 54). For instance, monocyte differentiation from a resting to an inflammatory state requires a shift in energy metabolism to high glucose consumption and rapid energy generation by glycolysis (55). Activated monocytes, along with dendritic cells, then produce IL-12, which is critical for the differentiation of Th1 lymphocytes that produce IFN-γ, a cytokine that activates splenic macrophages to eliminate Plasmodium-infected red blood cells (10, 53).
Importantly, we found that similarly to TNFR-/- mice, iNOS-deficient mice display impaired glycolytic metabolism in monocytic cells from Pc-infected mice. RNI can be produced by macrophages in response to priming by IFN-γ or/and activation triggered by TNF induced by microbial products via TLRs . As observed in TNFR-/- mice, iNOS deficiency resulted in increased parasitemia following Pc infection (56, 57). HIF-1α is required for the optimal expression of many glycolytic genes in macrophages, including those encoding GLUT1, hexokinase, phosphofructokinase and pyruvate kinase (43). Recently, it was suggested that RNI promotes S- nitrosylation at the Cys533 residue of HIF-1α (58), promoting its stabilization by direct inactivation of prolyl hydroxylase domains proteins (PHDs) (59). Indeed, the impairment of RNI release during Pc infection, resulted in reduced translocation of HIF-1α to the nuclei of splenic monocytes and non-parenchymal liver cells, which was markedly increased in infected WT mice. Therefore, we propose that the mechanism by which TNF signaling regulates HIF-1α stabilization/expression during Pc infection is mediated by RNI.
Different studies have demonstrated that HIF-1α is critical for host resistance to different pathogens (60), but both the participation of this transcription factor in malaria as well as the role of glycolytic metabolism in resistance to Plasmodium infection has not been previously explored. The HIF-1αΔLyz2 animals that are genetically deficient for HIF-1α, and have impaired glycolysis only in myeloid cells were more susceptible to Pc infection, further demonstrating the importance of myeloid cells in controlling parasitemia. This finding could be explained by the importance of glycolysis for the release of TNF and other pro-inflammatory cytokines by monocytes that are activated during Plasmodium infection (3, 40). Although we have found that the Pc infection-induced increase in nuclear translocation of HIF-1α was impaired in the absence of TNF signaling, HIF-1αΔLyz2 animals also displayed lower TNF secretion in response to Pc infection than WT mice. These observations suggest that the in vivo relationship between TNF signaling and HIF-1α-driven glycolysis is complex and that a positive feedback loop between TNF production, HIF-1α expression and glycolysis must exist during Pc infection.
The link between TNF and HIF-1α has been previously explored in different cell types. These studies demonstrated a crosstalk between NF-kB and HIF--1α. TNF signaling is known to recruit different intracellular adaptors that activate multiple signal transduction pathways. One class of these consists of the TRAF family of proteins, which can lead to IKK-dependent activation of NF-kB, thereby promoting inflammatory responses (61). Conversely, HIF-1α can also regulate NF-kB activation. Koedderitzch and collaborators have recently shown that in rheumatoid arthritis (RA) TNF induces a glycolytic shift via GLUT-1 and HIF-1α. As with malaria, TNF is a pivotal cytokine involved in the pathogenesis of RA. In vitro treatment of fibroblast-like synoviocytes with TNF induces upregulation of GLUT-1 and HIF-1α, which depends on TAK1-induced NF-kB activation downstream of TNF receptor 1 (62). NF-kB plays a central role in inflammatory responses and orchestrates the expression of TNF. Moreover, an NF-kB binding site is present in the proximal promoter site of the HIF-1α gene, indicating that NF-kB activation can regulate its expression (63, 64).
Plasmodium parasites do not synthesize glucose, being solely dependent on host glucose as a source of energy required for parasite replication. On the one hand, the increased glucose uptake and subsequent glycolytic metabolism in monocytes promote hypoglycemia, helping to starve the erythrocytic stage of the parasite, controlling its replication. On the other hand, hypoglycemia may also be detrimental to the host. For instance, lactate, the final product of glycolytic metabolism of glucose, when secreted in high amounts, causes acidosis with decreased blood pH and severe consequences to the host, such as extreme fatigue, pain, overall feelings of physical discomfort and decreased appetite (5).
Indeed, we found that the absence of TNF signaling and iNOS reverted the development of hypothermia, which is a known disease manifestation in Pc infection- induced experimental malaria in mice (65–67). Moreover, the attenuation of clinical symptoms in TNFR-/- infected mice extended beyond thermal regulation. Despite increased parasitemia, the animals displayed restored physical activity, food consumption, energy expenditure and respiratory exchange rate compared to WT animals. These findings demonstrate that TNFR-/- mice are more “tolerant” to disease. Thus, our results re-inforce the concept that TNF plays dual opposing roles in malaria promoting host resistance during malaria while promoting pathology.
In summary, the data reported here reveal that TNF-induced RNI induces HIF- 1α-mediated glycolysis in monocytes promoting host resistance to infection with Plasmodium. Hence, our findings address a fundamental mechanistic question concerning the pathophysiology of malaria infection in the vertebrate host. These findings may provide insights for developing novel therapeutic interventions to treat this devastating disease.
Materials and Methods
Mice
C57BL/6, iNOS, TNR1-/- and TNFR-/- (TNFR p55/p75 chains double knockout mice) were obtained from the Center for Breeding of Transgenic Mice from the Ribeirao Preto Medical School. Conditional knockout mice (TNFR1ΔLyz2 or HIF1aΔLyz2) were generated by crossing LysMCre+/- to TNFR1flfl or HIF1aflfl mice, which were maintained on a C57BL/6 genetic background. Mice used in experiments were sex and age-matched: female mice between 8–12 weeks of age. All mouse lineages were housed in micro-isolators in a maximum number of six mice per cage, in a specific pathogen-free facility at the Oswaldo Cruz Foundation, or Ribeirão Preto Medical School or University of Massachusetts Medical School, under controlled temperature (22–25°C) and 12-h light-dark cycle and provided with water and food ad libitum.
Ethics statement
All experiments were carried out in accordance with institutional guidelines for animal ethics and approved by the Ethics Committee on Animal Use of Ribeirão Preto Medical School University of São Paulo (CEUA 94 / 2020), Institutional Ethics Committees from Oswaldo Cruz Foundation (Fiocruz-Minas, CEUA/LW15/14, and LW16/18) and UMMS (IACUC A-1369-14-5), respectively.
Experimental Infections
Plasmodium chabaudi chabaudi AS strain (Pc) was used for experimental infections (69, 70). First, Pc was maintained in C57BL/6 mice by serial passages once a week up to eight times. For experimental infection, mice were injected intraperitoneally (i.p.) with 105 infected red blood cells (iRBCs) diluted in 100 µl PBS-1X. The percentage of RBCs containing parasites (parasitemia) was measured in the blood with Giemsa at different days post-infection (dpi).
Glucose Measurement
The glucose uptake was measured using 2-[14C]-deoxyglucose. Experiments were performed at the National Mouse Metabolic Phenotyping Center (MMPC) at UMASS Medical School. At 6 am on day 8 dpi, basal glucose uptake in individual organs was measured using an intravenous injection of 2-[14C]-deoxyglucose. After 1h, mice were anesthetized, and tissue samples were taken for organ-specific levels of 2- [14C]-deoxyglucose-6-phosphate. Blood glucose levels were measured with an Accu-chek glucometer.
Mouse RNA-Seq
RNA-seq was performed in biological replicates (3 mice per group). Liver samples were collected from C57BL/6 mice at different time points: 12 am; 6 am; 12 pm, and 6 pm from mice at day 8 post-infection with P. ch. or uninfected control. RNA-seq libraries were prepared using the TruSeq Stranded mRNA Kit (Illumina) following the manufacturer’s instructions. Briefly, poly-A-containing mRNA molecules were purified using poly-T oligo-attached magnetic beads and fragmented using divalent cations. The RNA fragments were transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen), followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. Finally, cDNA fragments then have the addition of a single ’A’ base and subsequent ligation of the adapter. The products were then purified and enriched by PCR using paired-end primers (Illumina) for 15 cycles to create the final cDNA library. The library quality was verified by fragmentation analysis (Agilent Technologies 2100 Bioanalyzer) and submitted for sequencing on the Illumina NextSeq 500 (Bauer Core Facility Harvard University). These samples were collected in according to the circadian cycle published by our group (32).
RNA extraction and Real-time PCR
Tissue fragments were resuspended in Trizol Reagente (Invitrogen) and total RNA extraction was performed (Promega) according to manufactureŕs instructions, and converted into cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to manufacturer’s instructions. Sybr Green PCR Master Mix (Applied Biosystems) was used to carry out the reactions in an QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) under standard conditions. qRT-PCR data were presented as 2-ΔΔCt.
Isolation of splenocytes and purification of CD11b+, B and T cells
Spleens from control and infected mice at 8 dpi were dissociated through a 100-μm nylon cell strainer for obtaining single-cell suspensions. For ex vivo cultures, known concentrations of splenocytes were resuspended in RPMI 1640 medium supplemented with penicillin, streptomycin, and 10% fetal bovine serum (FBS) (Gibco, ThermoFisher). In some experiments, splenocytes (2 × 106 cells) were stimulated in vitro with 1 μg/ml of LPS for 24h, or TNF-α [20ng/ml] or not during 15 minutes. For CD11b+ cell-, B cell- or T cell- purification, immunomagnetic beads for positive selection of CD11b+ cells, B cells and T cells (Miltenyi Biotec) were used, respectively, according to manufacturer’s instructions.
Hepatocyte’s isolation
First, the liver portal vein was cannulated with a 24G catheter. The inferior cava vein was cutted to allow perfusion flow. Perfusion was done with 50 mL of Hanks A and then 25 mL of Hanks B with 20 mg of collagenase. After that, tissue was dissociated and samples were filtered in a 40 mM membrane. Cells were centrifuged: 60x g (5 min, 4oC), disposing of supernatants, 60x g (5 min, 4oC), and resuspended in cold RPMI 10% SFB.
Liver non-parenchymal-cells
Livers were first digested in a collagenase solution (5 mg/liver in 10 mL of RPMI 2% SFB) at 37oC for 35 min. Then, the solution was added PBS/BSA 0,5% + EDTA in order to inactivate collagenase and cells were differentially separated by centrifugation: 300 x g (10 min, 4oC) disposing supernatants, 60 x g (3 min, 4oC) twice preserving supernatants and finally, 300 x g (10 min, 4oC) disposing supernatants. The samples were filtered through 100 μm cell strainers and centrifuged for 300 x g (10 min, 4oC). Red blood cells were lysed using ACK (ammonium chloride potassium) buffer. Concentrations of single-cell suspensions were then adjusted following counting on the hematocytometer.
Western blotting
Mouse splenocytes, purified CD11b+ splenic cells, isolated hepatocytes, isolated non-parenchymal cells, or liver total from infected or not mice were lysed with RIPA buffer solution (Sigma) with a protease and phosphatase inhibitor cocktail (ThermoFisher). After 15 min on ice, lysates were centrifuged at 13,000 × g for 10 min at 4 °C. The proteins were separated in a 10%-acrylamide NUPAGE Bis-tris Protein gels (Invitrogen) and transferred onto nitrocellulose membranes. Membranes were blocked with 5% (wt/vol) nonfat milk (Molico) in Trisbuffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and then incubated overnight at 4°C with primary antibodies. The membranes were incubated with GLUT1 (Abcam, 1:1000), GLUT2 (Abcam, 1:1000), Na,K-ATPase (Cell Signaling, 1:2000) and β-actin (Sigma, 1:5000) specific antibodies. Subsequently, membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad) and then the chemiluminescence signal was recorded on the iBright FL1500 (ThermoFisher). Data were analyzed with iBright FL1500.
Metabolic Profiling
Metabolic profiling of CD11b+ cells, B cells, T cells, hepatocytes, and non- parenchymal cells was undertaken using a Seahorse XFe96 Extracellular Flux Analyzer (Agilent) in microplates. Basal oxygen consumption rate (OCR) and extracellular acidification production (ECAR) were measured by Phenotypic Seahorse Kit (KIT 103325-100), or Seahorse XF Cell Mito Stress Tests (KIT 103015- 100), or Glycolytic Rate Assay Kit (KIT 103344-100) according to the manufacturer’s instructions.
Flow cytometry
Splenocytes (2 × 106 cells) from mice at 0 (uninfected controls) and 8 days post- infection were stained with fluorophore-labeled monoclonal antibodies (mAbs) specific for cell surface markers. The following flow cytometry specific mAbs were used: CD11c-PECy7 clone N418 (1:200), CD11b-APCCy7 clone M1/70 (1:200), Ly6c PERCP clone H.K1.4 (1:200), F4/80- PE clone BM8 (1:200), Ly6g FITC clone 1A8 (1:200), CD45 Pacific Blue- clone30F-11 (1:200), CD3 FITC clone 145-2c11 (1:200), CD4 PE clone RM4-5 (1:200), CD8 PERCP clone 53-6.7 (1:200), CD19 PeCy7 clone 6D5(1:200) and Bv510 (Live/Dead kit, ThermoFisher). Intracellular staining of GLUT1 Alexa Fluor 647 [EPR3915] (1:100) was performed following cell fixation and permeabilization using the eBioscience Foxp3 Fixation/Permeabilization Kit. Data were acquired in a FACSCanto II machine (BD Biosciences) and analyzed using FlowJo software (Tree Star).
Cytokine measurement
Plasma was collected from whole blood and supernatants were collected from cell cultures. Cytokine concentration in these samples were determined using CBA mouse Inflammation Kit (BDTM) or ELISA according to the manufacturers’ instructions (Biolegend).
Food Intake and Physical Activity
Mouse food intake and physical activity were assessed for 3 days prior to infection and at days 6, 7 and 8 dpi with Pc using metabolic cages (TSE Systems, Chesterfield, MO) located at the National Mouse Metabolic Phenotyping Center (MMPC) at UMASS Medical School. Mice were housed under controlled temperature and lighting, with food and water ad libitum. Food intake and physical activity monitoring was fully automated using the TSE Systems LabMaster platform. LabMaster cages allowed the use of bedding, thus, minimizing any animal anxiety during the experimental period. Physical activity was calculated based on quantitative measurement of horizontal and vertical movement (XYZ-axis). In addition, non-invasive measures of O2 consumption and CO2 production were used to calculate the respiratory exchange ratio to reflect energy expenditure.
RNA-Seq Analysis
Gene expression of each experimental group was shown at different time points 12 am; 6 am; 12 pm and 6 pm. The average expression of all time points was calculated for visualization in heatmaps. The heatmaps presented in Figure 3 was developed with a free version of the software Morpheus (https://software.broadinstitute.org/morpheus). For hierarchical clustering, we used linkage from the average expression of each gene in metric One Minus Pearson Correlation.
Statistical analysis
GraphPad Prism 7.0 software was used for statistical analysis. Multiple-group comparisons were performed with either one-way ANOVA (followed by Tukey’s post hoc test) or two-way ANOVA (followed or not by Sidak’s post hoc test). Unpaired two-tailed Student’s t-test was used for the comparison of two conditions. Results are expressed as means ± SEM. P value ≤ 0.05 was considered significant.
Acknowledgements
The authors thank members of the D.L.C., J.S.S., J.C.A.F. and R.T.G. groups for scientific discussions and technical help. Denise Brufato Ferraz and Francielle Pioto for the excellent technical assistance.
Funding
This work was supported by the Fundação de Amparo de Pesquisa do Estado de São Paulo (2016/23618-8) (2020/01043-9), the National Institute of Infectious Diseases and Allergy (1R21 AI150546-01, R01NS098747 and R01AI079293) and the Instituto Nacional de Ciência e Tecnologia de Vacinas (CNPq/ FAPEMIG/ CAPES 465293/2014-0).
Competing interests
The authors declare no competing interests.
Data and materials availability
GEO (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE109908) accession number is GEO: GSE109908.
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