Abstract
Cichlid fishes inhabiting the East African Great Lakes, Victoria, Malawi, and Tanganyika, are textbook examples of parallel evolution, as they have acquired similar traits independently in each of the three lakes during the process of adaptive radiation. In particular, “hypertrophied lip” has been highlighted as a prominent example of parallel evolution. However, the underlying molecular mechanisms remain poorly understood. In this study, we conducted an integrated comparative analysis between the hypertrophied and normal lips of cichlids across three lakes based on histology, proteomics, and transcriptomics. Histological and proteomic analyses revealed that the hypertrophied lips were characterized by enlargement of the proteoglycan-rich layer, in which versican and periostin proteins were abundant. Transcriptome analysis revealed that the expression of extracellular matrix-related genes, including collagens, glycoproteins and proteoglycans, was higher in hypertrophied lips, regardless of their phylogenetic relationships. In addition, the Wnt signaling pathway, which is involved in promoting proteoglycan expression, was highly expressed in both the juvenile and adult stages of hypertrophied lips. Our comprehensive analyses showed that hypertrophied lips of the three different phylogenetic origins can be explained by similar proteomic and transcriptomic profiles, which may provide important clues into the molecular mechanisms underlying phenotypic parallelisms in East African cichlids.
Introduction
Parallel evolution is evidence of natural selection that drives adaptive solutions to the environment (Losos, 2011). Acquisition of similar traits in distantly related lineages can be observed in multiple lineages, such as the loss of eyes in cave fish, patterns of wing colors in Heliconius butterflies, and body armor of sticklebacks (Krishnan and Rohner, 2017; Stern, 2013). It is considered that the same direction of natural selection drives phenotypic changes among species since it is unlikely that these complex phenotypes have been acquired repeatedly just by neutral evolution (Losos, 2011; Stern, 2013). Therefore, research on parallel evolution enables us to compare the commonalities and differences in the mechanisms behind the acquisition of novel traits and elucidate how adaptation occurs in similar environments.
East African cichlids offer one of the best opportunities to examine the molecular basis of parallel evolution (Kocher et al., 1993; Muschick et al., 2012). There are hundreds of endemic cichlid species in the East African Great Lakes, Victoria, Malawi, and Tanganyika, as a result of explosive adaptive radiation (Salzburger, 2018; Santos et al., 2023). As a result of independent adaptations in each lake, parallel evolution is often observed among lakes, such as that of the nose snout (Duenser et al., 2023), jaw morphology, and body shape (Kocher et al., 1993; Muschick et al., 2012). To reveal their evolutionary mechanisms, genetic bases of the phenotypes have been investigated. For example, the parallel evolution of body stripes in cichlids of Lake Victoria and Lake Malawi was explained by relatively simple changes in pigmental cell arrangement caused by mutations in the cis-regulatory element of agouti-related peptide 2 (agrp2) (Kratochwil et al., 2018). Although this parallel evolution of body stripe patterns can be explained by a single gene, most traits are polygenic, hindering elucidation of the parallel evolution mechanism.
Cichlid fishes have acquired a huge diversity in their oral morphologies, reflecting ecological and trophic specializations (Albertson and Kocher, 2006; Kocher, 2004; Lecaudey et al., 2021, 2019). Hypertrophied lips, one of the most prominent examples of oral diversity, have been independently acquired in cichlids inhabiting each of the East African Great Lakes, as well as in the Nicaraguan crater lakes and South American rivers (Henning et al., 2017; Kautt et al., 2020; Masonick et al., 2023, 2022). Several hypotheses have been proposed for the adaptive roles of hypertrophied lips, such as for effectively sucking prey out from narrow crevices (Lukas et al., 2015), reducing mechanical stress during foraging in rocky habitats (Fryer, 1972; Greenwood, 1974), and facilitating prey detection by providing an enlarged area for taste receptors (Arnegard and Snoeks, 2001; Oliver and Arnegard, 2010). Thus, hypertrophied lips are considered an adaptive trait for a specific trophic niche, making them an ideal model for understanding the mechanism of parallel evolution.
The molecular mechanisms underlying hypertrophied lips have been investigated in various lineages of cichlids. For example, transcriptome comparisons between hypertrophied and normal lips of cichlids in Lake Tanganyika (Colombo et al., 2013) and South American crater lakes (Manousaki et al., 2013) have identified some differentially expressed genes (DEGs) as candidates for lip hypertrophy; however, their primary regulators remain to be elucidated. In addition, quantitative trait locus (QTL) mapping (Henning et al., 2017) and genome-wide association study (GWAS) (Masonick et al., 2023) have been conducted to elucidate the genomic loci responsible for hypertrophied lips in the cichlids of Lake Victoria and Lake Malawi. These genome-wide studies concluded that lip hypertrophy was a polygenic trait involving several candidate genes. However, the molecular mechanism underlying lip hypertrophy has not yet been elucidated.
In this study, we comprehensively compared the hypertrophied lips of cichlids across all East African Great Lakes using histology, proteomics, and transcriptomics. Histological and proteomic analyses revealed a distinct microstructure of hypertrophied lips compared to normal lips, and primary candidate proteins were identified. Transcriptome analysis at different developmental stages showed that the Wnt signaling pathway was highly expressed in cichlids with hypertrophied lips at both the juvenile and adult stages. It is noteworthy that the distinct expression profiles observed in the proteome and transcriptome analyses of hypertrophied lips were similar among cichlids from each of the East African Great Lakes. The present study, which integrates comprehensive analyses for cichlids from all East African Great Lakes, provides insight for a better understanding of the molecular basis of a typical example of parallel evolution.
Materials and Methods
Aminals
Three species of Victoria cichlids (Haplochromis chilotes with hypertrophied ips; H. sauvagei, and H. pyrrhocephalus with normal lips , four species of Malawi cichlids Placidochromis milomo with hypertrophied lips; Maylandia lombardoi, M. zebra, and abidochromis caeruleus with normal lips), and two species of Taganyika cichlids Lobochilotes labiatus with hypertrophied lips; Tropheus moorii with normal lips) were sed in the experiments. H. chilotes, H. sauvagei, and H. pyrrhocephalus were collected rom the Mwanza gulf of Lake Victoria during an exploration that was led by the Nikaido aboratory in 2018. The other fish were obtained from a commercial supplier. Fish were maintained and bred at 27°C on a 12/12 h light/dark cycle. One to 12 individuals were ept in a plastic tank (40 cm × 25 cm × 36 cm) and fed food pellets 2-3 times a day. The ample information is provided in Table S1. All experiments were conducted in accordance with the Institutional Animal Experiment Committee of the Tokyo Institute of Technology.
Histological observation
Cichlids were anesthetized with 0.02% ethyl p-aminobenzoate. The lower lips were then dissected, along with the lower jaw, and were fixed in 4% paraformaldehyde (PFA, Fujifilm-Wako, Chuo-ku, Osaka, Japan) in phosphate-buffered saline (PBS) at 4°C for 24 h. Tissues were demineralized in 0.34 M ethylenediaminetetraacetic acid (EDTA, Fujifilm-Wako, Chuo-ku, Osaka, Japan) in 100 mM, pH7.0 tris (hydroxymethyl) aminomethane (Tris, Fujifilm-Wako, Chuo-ku, Osaka, Japan) buffer at 4°C for 24 h. After demineralization, tissues were treated with 20% sucrose/PBS at 4°C for 24 h. Tissues were then embedded in Tissue-Tek O.C.T. compound (Sakura Finetek, Chuo-ku, Tokyo, Japan) and frozen with liquid nitrogen. Embedded blocks were sliced in the sagittal plane into 20 µm sections and placed on a coated glass slide (MAS-01, Matsunami, Bunkyo-ku, Tokyo, Japan). Sections were stored at −80°C until they were used for experiments. To observe the histological structure, hematoxylin–eosin (HE) staining, Alcian blue (AB) staining, and Van Gieson (VG) staining were conducted. Mayer’s hematoxylin solution (Fujifilm-Wako, Chuo-ku, Osaka, Japan), Eosin Y solution (Fujifilm-Wako, Chuo-ku, Osaka, Japan), ALCIAN BLUE 8GX (Sigma-Aldrich, St. Louis, MO, USA), Van Gieson solution P (Fujifilm-Wako, Chuo-ku, Osaka, Japan), and Van Gieson solution F (Fujifilm-Wako, Chuo-ku, Osaka, Japan) were used for each staining. Experiments were performed according to the manufacturer’s protocol and previous studies (Arnegard and Snoeks, 2001; Oliver and Arnegard, 2010). For AB staining, the pH was adjusted to 2.5 with 3% acetic acid (Fujifilm-Wako, Chuo-ku, Osaka, Japan). For VG staining, Van Gieson solution P and Van Gieson solution F were prepared at a ratio of 7.55:1. The stained area was calculated using ImageJ version 1.5.1 (Schneider et al., 2012) after trimming the image to include only the lips. The images were captured using a Zeiss Axioplan fluorescence microscope and a Zeiss Axiocam 503 color CCD camera (Carl Zeiss, Oberkochen, Germany). Images were level-corrected, contrast adjusted, and tiled using Adobe Photoshop CC 2022.
Chondroitinase digestion
Chondroitinase ABC from Proteus vulgaris (Sigma-Aldrich, St. Louis, MO, USA) was used for chondroitinase digestion. 2 mUnits/0.1% bovine serum albumin solutionBSA in 20 mmol/L Tris-HCl pH 7.7 were prepared as the enzyme solution. In the experiment, the sections were first treated with 100 μL of 0.1% BSA solution for 5 min. Next, 100 μL of enzyme solution, or BSA solution for the control, was added. During the reaction, the sections were placed in a moist chamber. The enzymatic reaction was performed at 37°C for 1 h. After the reaction, the samples were washed with water for 15 min, and then AB staining was examined.
Identification of extracellular matrix (ECM)-related genes in cichlids
To compare the transcriptome of cichlid lips, cichlid extracellular matrix (ECM)-related genes were identified using the data from a previous study (Hynes and Naba, 2012). The amino acid sequences of human, mouse, and zebrafish ECM-related genes registered in Human GENCODE 41, Mouse GENCODE M30, Ensembl, and UniProtKB were used as queries, and those of M. zebra and O. niloticus were identified from their protein sequences registered in Ensembl and Uniprot using OrthoFinder2 v.2.5.4. The longest amino acid sequence of each isoform was selected to represent the gene. After Orthfinder2 analysis, ortholog correspondences were corrected manually, if necessary. A list of genes is provided in Table S2.
Sample preparation for proteomic analysis
Proteomic analysis was conducted on the lips of cichlids, H. chilotes (hypertrophied) and H. sauvagei (normal), from Lake Victoria. The lips of H. chilotes were analyzed separately for the base and tip. For proteomics analysis of pellet fractions, dissected samples were homogenized with a pestle in 1% sodium dodecyl sulfate in PBS supplemented with a protease inhibitor cocktail (cOmpleteTM mini, EDTA free, Roche, Basel, Switzerland). The resultant lysate was centrifuged at 20,000× g for 15 min, and the supernatant was extracted. A fourfold volume of methanol, an equal volume of chloroform, and a threefold volume of distilled water were added to the supernatants, and the suspension was vortexed vigorously for 1 min after each addition. After vortexing, the supernatants were centrifuged at 15,000× g for 2 min and the upper water layer was removed. A threefold volume of methanol was added, and the samples were centrifuged at 15,000× g for 2 min. The supernatant was removed by aspiration and the pellet fraction was obtained.
Subsequently, sample preparation for LC−MS/MS was performed according to a previous study (Masuda et al. 2009), with some modifications. Proteins in the pellet fraction were solubilized in phase transfer surfactant (PTS) solution (12 mM sodium deoxycholate, 12 mM sodium lauryl sulfate, 100 mM Tris-HCl, pH 9.0), and the protein concentration was determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Then, 50 μg of protein in 50 μL of PTS was prepared for the following procedure. First, the proteins were reduced by treatment with 5 mM dithiothreitol (DTT) at room temperature for 30 min, and then alkylated with 50 mM iodoacetamide in the dark at room temperature for 30 min. The reaction was stopped by a fivefold dilution with 50 mM ammonium bicarbonate. Denatured proteins in the solution were digested into peptide fragments in two steps: first, incubation with 0.5 μg of Trypsin/Lys-C Mix (Promega, Madison, WI) at room temperature for 3 h, followed by incubation with 1.0 μg of Trypsin/Lys-C Mix at 37°C overnight. After digestion, an equal volume of ethyl acetate and 0.5% trifluoroacetic acid (final concentration) were added and the mixture was shaken vigorously for 2 min. The mixture was then centrifuged at 15,700 × g for 2 min and the upper ethyl acetate layer was removed. The solvent was dried using a centrifugal evaporator. The residual pellet was redissolved in 600 μL of 0.1% TFA and 2% acetonitrile and then desalted using a handmade StageTip composed of an SDB-XC Empore Disk (3 M, Maplewood, MN, USA). The solution was applied to a StageTip equilibrated with 0.1% TFA and 2% acetonitrile, washed with 0.1% TFA and 2% acetonitrile, and eluted with 0.1% TFA and 80% acetonitrile. After the desalting, the solvent was dried using a centrifugal evaporator, and the residual peptides were redissolved in 120 μL of 0.1% TFA and 2% acetonitrile. The solution was centrifuged at 20,000 × g for 5 min, and the supernatant was collected and subjected to LC–MS/MS measurement.
LC–MS/MS measurement and data processing
LC–MS/MS measurements and SWATH-MS acquisition (Gillet et al., 2012) were performed using an Eksigent NanoLC Ultra and TripleTOF 4600 tandem-mass spectrometer or an Eksigent nanoLC 415 and TripleTOF 6600 mass spectrometer (AB Sciex, USA). The trap column used for nanoLC was a 5.0 mm × 0.3 mm ODS column (L-column2, CERI, Bunkyo-ku, Tokyo, Japan), and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos, Bunkyo-ku, Tokyo, Japan). The flow rate of nanoLC was 300 nL/min, and peptides were separated by applying a 10-40% linear acetonitrile gradient over 70 min in the presence of 0.1% formic acid. Data were acquired in DIA/SAWTH (Gillet et al., 2012) mode controlled by Analyst software (version 1.7, AB Sciex, USA) with the following settings: 15 Da fixed width window in 350–1100 m/z (50 cycles), 45 msec acquisition time, and 100–1600 fragment ion m/z range. The measurements were performed three times for each sample. Quantitative values were calculated by DIA-NN software (version 1.8.1) with default settings (Demichev et al., 2020). The library for DIA/SWATH quantification was generated using DIA-NN with the amino acid sequences of M. zebra total proteins obtained from UniProtKB. The P-value and fold change were calculated by Python libraries (scipy.stats and pandas) using the proteins detected in all three measurements for three parts of the lips. Quantitative values were scaled by log10 before statistical tests (Welch’s t-test, two sided).
RNA-seq analysis
RNA was extracted from dissected lower lips using TRI Reagent (Molecular Research Center, Inc.). Three species of Lake Victoria cichlids (Haplochromis chilotes [n = 4], H. sauvagei [n = 4], H. pyrrhocephalus [n = 2]), three species of Lake Malawi cichlids (Placidochromis milomo [n = 5], Maylandia zebra [n = 2], Labidochromis caeruleus [n = 5]), and two species of Lake Taganyika cichlids (Lobochilotes labiatus [n = 4], Tropheus moorii [n = 4]) were used for RNA-seq (Table S1). The extracted total RNA was sequenced at 100 bp paired-end reads on a NovaSeq 6000 by Macrogen Japan Corp., using a TruSeq stranded mRNA Library Prep Kit. The raw FASTQ data were qualified using fastp version 0.20.1 (Chen et al., 2018) in default option. The high-quality reads were mapped to M. zebra genome (Conte and Kocher, 2015) using STAR version 2.7.10a (Dobin et al., 2013) and quantified using rsem-calculate-expression version 1.3.1 (Li and Dewey, 2011). Principal component analysis (PCA) was conducted using Python scikit-learn package. Low-expression genes (average transcripts per kilobase million (TPM) below 5.0) were removed, and expression rates were normalized by Z score. The resulting 12,231 genes were used for PCA analysis. For PCA of ECM-related genes, we used 186 genes as inputs, which were extracted in the same manner. DEGs were detected using the TCC package (Sun et al., 2013) with the TMM-edgeR-edgeR combination. Enrichment analysis was conducted using DAVID version 2023q3 (Huang et al., 2009a, 2009b) after the gene IDs of M. zebra were converted to human gene IDs using ensembl biomart. The annotation terms used for functional annotation clustering were collected from biological process (Gene Ontology), KEGG, and Reactome.
Results
Comparative histology of hypertrophied and normal lips among cichlids from the East African Great Lakes
We compared the histological structures of hypertrophied and normal lips among cichlids from Lake Victoria, Lake Malawi, and Lake Tanganyika. For an accurate comparison, we first observed serial sections of the lower lips with the jaws to determine the midline of the lips in each species, which was defined by the shape of the lower dentary bone (Figure S1). Based on this definition, we compared the distribution of collagens and proteoglycans, the main ECM components, at the midline of the lower lips using VG stain (red) and AB stain (blue). Hypertrophied lips of cichlids from all lakes generally consisted of two mutually exclusive structures in the hypodermis: a collagen-rich layer at the base and a proteoglycan-rich layer at the tip. Moreover, comparison of hypertrophied lips and normal lips showed that the area of the proteoglycan-rich (AB positive) layer was notably enlarged in hypertrophied lips, which was statistically significant in cichlids from Lake Victoria. (Figure 1; Figure S2).
Proteoglycans and their related proteins were abundant in the hypertrophied lips
To explore the proteins associated with lip hypertrophy, we used shotgun proteomics to compare the protein profiles of hypertrophied lips and normal lips in Lake Victoria cichlids. Using LC–MS/MS in DIA/SWATH acquisition mode, we detected 2,720 proteins in total from three types of lip samples: the tip of hypertrophied lips (proteoglycan-rich loose connective tissue), the base of hypertrophied lips (collagen-rich loose connective tissue), and normal lips. First, we observed few differences between the tip of hypertrophied lips and the base of the hypertrophied lips (Figure S3). On the other hand, we identified 138 differentially accumulated proteins between the tip of hypertrophied and normal lips (133 proteins upregulated and 5 proteins downregulated) under the criteria (P-value < 0.05; log2 fold change > 1) (Figure 2A). Compared to normal lips, hypertrophied lips are abundant in proteoglycans and their related proteins such as versican a/b (vcan), brevican (bcan), periostin (postn), hyaluronan and proteoglycan link protein 1 (hapln1), and hyaluronan binding protein 2 (habp2), which were highly accumulated in hypertrophied lips with statistical significance (Figures 2B-D). The results of the above proteomic analyses were consistent with the histological observation that proteoglycan-rich loose connective tissue was enlarged in hypertrophied lips compared to that in normal lips.
Chondroitin sulfate proteoglycans such as vcan are the main component of hypertrophied lips
Next, we performed glycosaminoglycan digestion experiments to examine whether loose connective tissue was composed of the proteoglycans, which were detected as highly accumulated proteins in hypertrophied lips. Among the proteoglycans, we focused on vcan, which showed a clear difference in protein amount between hypertrophied and normal lips. Vcan is a high-molecular-weight core protein (approximately 370 kDa) that is glycosylated with chondroitin sulfate (Iozzo and Schaefer, 2015; Wight et al., 2020). Therefore, we stained sections of hypertrophied lips with AB stain after the treatment with chondroitinase, which specifically digests glycosylated chondroitin sulfate. As a result, AB staining in loose connective tissue became drastically weak in treated sections, which was common among lakes (Figures 2E-G). Our data suggested that the main components of enlarged loose connective tissue in hypertrophied lips are chondroitin sulfate proteoglycans such as vcan.
Convergent expression in ECM-related genes
Given that proteomic analysis is not well suited for detecting high-turnover proteins (Decaris et al., 2014), we additionally conducted transcriptomic comparisons between hypertrophied and normal lips across Lake Victoria, Lake Malawi and Lake Tanganyika. PCA with all expressed genes clustered according to each lake, reflecting genetic closeness (Figure 3A). Next, we compared the gene expression of ECM-related genes, for which the proteomics profiles were clearly distinct between hypertrophied and normal lips. A PCA plot was drawn using only ECM-related genes in cichlids, which were identified based on a previous study (Hynes and Naba, 2012) (Figure 3B). In contrast to the PCA plot of all expressed genes, that of ECM-related genes reflected differences of lip morphology (hypertrophied and normal), regardless of the genetic closeness. To evaluate the results of these PCAs, we calculated the average silhouette (AS) score, which assesses the cohesiveness of the cluster. In the PCA of all genes, the AS score of the cluster by lake (0.1601) was higher than that of the cluster by lip morphology (0.0849). In contrast, in the PCA of ECM-related genes, the AS score of the cluster by lip morphology (0.1798) was higher than that of the cluster by lake (0.0774). These results indicate that hypertrophied lips are characterized by the expression of ECM-related genes.
Next, we compared the expression of each ECM-related gene between hypertrophied and normal lips of cichlids from the three lakes (Figures 3C, D). As a result, several ECM-related genes showed higher expression in hypertrophied lips than in normal lips, such as type-I collagen fibrils (col1a1a, col1a1b, and col1a2), hyaluronan-binding proteoglycans (vcanb, hapln1a/b, and hapln3), periostins (postna/b), small leucine-rich proteoglycans (dcn, lum, and aspn), and microfibril-related genes (mfap2, emilin1b, emilin2a, emilin2b, and emilin3b). Consistent with the proteomics comparison, some proteoglycans and their related proteins (vcanb, hapln1a, and postna) showed significantly higher expression in hypertrophied lips than in normal lips. In conclusion, we observed convergent expression of ECM-related genes in hypertrophied lips of cichlids among the East African Great Lakes.
High expression of ECM-related genes in the juvenile and adult stages of hypertrophied lips
Next, we performed transcriptome analysis by comparing juvenile and adult cichlids from Lake Victoria to examine the possible expression changes in ECM-related genes during development (Figure 4A). In the adult stage, the expression profiles of ECM-related genes between hypertrophied and normal lips showed obvious differences (low correlation value 0.75; orange square in Figure 4B), which was consistent with previous analyses (Figure 3B). In the juvenile stage, the difference was weaker than in adult stage (0.85; blue square in Figure 4B). Interestingly, the difference in the expression profiles between the juvenile and adult stages of hypertrophied lips (0.90; Figure 4B black square) was lower than that of normal lips (0.83; Figure 4B black dotted square). To examine the genes contributing to the differences in expression profiles, we performed hierarchical clustering analysis for the relative expression levels of each ECM-related gene (Figure 4C, D). The analyses consistently showed the higher expression of proteoglycans and their related genes (e.g. postna, vcanb, and hapln1b) in hypertrophied lips at both the juvenile and adult stages. These results indicated that the accumulation of proteoglycans, which leads to the lip hypertrophy, begins at the juvenile stage.
The Wnt pathway-related genes are highly expressed in hypertrophied lips
To explore the molecular pathways involved in lip hypertrophy, we performed an enrichment analysis using DAVID (Huang et al., 2009a, 2009b) against DEGs obtained from the transcriptome. The results of the enrichment analysis suggested that the genes that were categorized into both canonical and non-canonical Wnt signaling pathways, were highly expressed in the hypertrophied lips of juvenile and adult cichlids. Comparison of the developmental stages revealed differences in the expression patterns between the canonical and noncanonical Wnt pathways: the genes of the canonical Wnt pathway were highly expressed in both stages (Figures 5C–I), whereas those of the non-canonical pathway were highly expressed only in the adult stage (Figures 5J–N). We further compared the expression patterns of these Wnt signaling pathways in cichlids from Lake Malawi and Lake Tanganyika. Notably, the expression of genes for the Wnt pathway tend to be higher in the hypertrophied-lip cichlids in Lake Malawi and Lake Tanganyika, although the differences were not as distinct as those found in cichlids from Lake Victoria. These results were consistent with those of previous studies that showed that the expression of ECM-related genes, such as vcan, was promoted by the Wnt signaling pathway (Rahmani et al., 2005). Continuous activation of the Wnt signaling pathway throughout all developmental stages may lead to lip hypertrophy via the deposition of ECM-related proteins.
Discussion
In this study, we investigated the differences between hypertrophied and normal lips using histology, proteomics, and transcriptomics. Histological comparisons revealed that cichlids with hypertrophied lips from all three East African Great Lakes exhibited an enlargement of the proteoglycan-rich loose connective tissue. By integrating proteomics and transcriptomics, we identified several proteins that were significantly accumulated in the hypertrophied lips. Among them, proteoglycans and their related proteins such as vcan and postn, could be a primary factor that explains lip hypertrophy. Interestingly, these proteins are known to be involved in the formation of human keloids, a skin disease characterized by an overabundance of ECM in the dermis (Deng et al., 2021; Naitoh et al., 2005). It has been discussed that excessive deposition of vcan retains large amounts of water in the sulfate or carbonyl moieties, contributing to the increase in keloid volume (Yagi et al., 2013). In addition, postn is involved in fibrosis through its ability to interact with multiple ECM proteins. For example, upregulation of postn leads to strengthening of the ECM by crosslinking with collagens and excessive deposition of ECM proteins by differentiating quiescent fibroblasts into activated myofibroblasts (Ashley et al., 2017; Yamaguchi et al., 2013). In cichlid, vcan and postn may have a similar function, leading to hypertrophic loose connective tissue.
The Wnt signaling pathway was also elevated in both the adult and juvenile stages of hypertrophied lips. The Wnt signaling pathway is known to be a key regulator of the expression of ECM-related genes; for example, vcan is directly regulated by the Wnt/b-catenin and tcf4 signaling pathways, leading to vcan upregulation (Rahmani et al., 2005). Additionally, Wnt5a, a noncanonical pathway, plays a critical role in dermal condensation during mouse and sugar glider development (Feigin et al., 2023). Cellular-level localization of gene expression for the canonical and non-canonical Wnt pathway is required for future examination of the involvement of the Wnt signaling pathway in lip hypertrophy.
Although the parallel evolution of hypertrophied lips in cichlids has received considerable attention in evolutionary biology, the commonality and diversity underlying their molecular basis remain poorly understood. This might be primarily due to the fact that many genetic factors are possibly involved in the dynamic organization of hypertrophied lips. We uncovered the apparent relationships between hypertrophied lips and the expression profiles of ECM proteins, in particularly proteoglycans. The trends for the overall expression of ECM-related genes were similar across hypertrophied lip species, but we rarely observed a specific gene that was commonly expressed at high or low levels in all three examples of hypertrophied lips across all East African Great Lakes. Furthermore, although we focused primarily on the relationship between the Wnt signaling pathway and lip hypertrophy, several other pathways may be involved in the development of hypertrophied lips. These findings imply that although enlargement of proteoglycan-rich loose connective tissue is common in hypertrophied lips, the developmental pathways to accomplish this are diverse in each lake. The pathways responsible for lip hypertrophy therefore require further investigation. Our approach of integrating histology, proteomics and transcriptomics revealed complex evolutionary pathways for hypertrophied lips in cichlids of the East African Great Lakes, providing an opportunity to further understand the molecular mechanism underlying the parallel evolution of polygenic traits, such as jaw morphology or body shape.
Acknowledgements
This study was supported by JSPS KAKENHI (23KJ0951 to N. M., 17H04606, 20KK0167 to M.N.) and JST SPRING (JPMJSP2106). Computations were partially performed on the NIG supercomputer at ROIS National Institute of Genetics. The authors thank the Open Research Facilities for Life Science and Technology, Tokyo Institute of Technology for LC–MS/MS analysis (for technical assistance). The authors thank the Hongoh laboratory members. Nagatoshi Machii thank my family, Nagashi Machii, Mistue M. and Sawa M.
Data availability
RNA-seq short reads and raw proteome data are stored at NCBI and jPOST (Okuda et al., 2017) (Accession: PRJDB3401, PRJDB17757, and JPST003042/PXD051571).
References
- Genetic and developmental basis of cichlid trophic diversityHeredity 97:211–221
- New Three-Spotted Cichlid Species with Hypertrophied Lips (Teleostei: Cichlidae) from the Deep Waters of Lake Malaŵi/NyasaAfrica. The American Society of Ichthyologists and Herpetologists https://doi.org/10.1643/0045-8511(2001)001[0705:NTSCSW]2.0.CO;2
- Periostin regulates fibrocyte function to promote myofibroblast differentiation and lung fibrosisMucosal Immunol 10:341–351
- . fastp: an ultra-fast all-in-one FASTQ preprocessorBioinformatics 34:i884–i890
- The ecological and genetic basis of convergent thick-lipped phenotypes in cichlid fishesMol Ecol 22:670–684
- An improved genome reference for the African cichlid, Metriaclima zebraBMC Genomics 16
- Proteomic analysis of altered extracellular matrix turnover in bleomycin-induced pulmonary fibrosisMol Cell Proteomics 13:1741–1752
- DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughputNat Methods 17:41–44
- Single-cell RNA-seq reveals fibroblast heterogeneity and increased mesenchymal fibroblasts in human fibrotic skin diseasesNat Commun 12
- STAR: ultrafast universal RNA-seq alignerBioinformatics 29:15–21
- Conserved Molecular Players Involved in Human Nose Morphogenesis Underlie Evolution of the Exaggerated Snout Phenotype in CichlidsGenome Biol Evol 15https://doi.org/10.1093/gbe/evad045
- Convergent deployment of ancestral functions during the evolution of mammalian flight membranesSci Adv 9
- The cichlid fishes of the Great Lakes of AfricaTheir Biology and Evolution 641
- Targeted Data Extraction of the MS/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis*Mol Cell Proteomics 11
- Cichlid fishes of Lake VictoriaEast Africa: the biology and evolution of a species flock. Bull Brit Mus (Nat Hist) Zool Suppl 6:1–134
- Genetic dissection of adaptive form and function in rapidly speciating cichlid fishesEvolution 71:1297–1312
- Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene listsNucleic Acids Res 37:1–13
- Systematic and integrative analysis of large gene lists using DAVID bioinformatics resourcesNat Protoc 4:44–57
- Overview of the matrisome--an inventory of extracellular matrix constituents and functionsCold Spring Harb Perspect Biol 4
- Proteoglycan form and function: A comprehensive nomenclature of proteoglycansMatrix Biol 42:11–55
- Contrasting signatures of genomic divergence during sympatric speciationNature 588:106–111
- Similar Morphologies of Cichlid Fish in Lakes Tanganyika and Malawi Are Due to ConvergenceMol Phylogenet Evol. 2:158–65
- Adaptive evolution and explosive speciation: the cichlid fish modelNat Rev Genet 5:288–298
- Agouti-related peptide 2 facilitates convergent evolution of stripe patterns across cichlid fish radiationsScience 362:457–460
- Cavefish and the basis for eye lossPhilos Trans R Soc Lond B Biol Sci 372https://doi.org/10.1098/rstb.2015.0487
- Transcriptomics unravels molecular players shaping dorsal lip hypertrophy in the vacuum cleaner cichlid, Gnathochromis permaxillarisBMC Genomics 22
- Molecular mechanisms underlying nuchal hump formation in dolphin cichlid, Cyrtocara mooriiSci Rep 9
- RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genomeBMC Bioinformatics 12
- Convergence, adaptation, and constraintEvolution 65:1827–1840
- What big lips are good for: on the adaptive function of repeatedly evolved hypertrophied lips of cichlid fishesBiol J Linn Soc Lond 115:448–455
- Parsing parallel evolution: ecological divergence and differential gene expression in the adaptive radiations of thick-lipped Midas cichlid fishes from NicaraguaMol Ecol 22:650–669
- A Kiss of Deep Homology: Partial Convergence in the Genomic Basis of Hypertrophied Lips in Cichlid Fish and Human Cleft LipGenome Biol Evol 15https://doi.org/10.1093/gbe/evad072
- Phylogenomic Analyses Show Repeated Evolution of Hypertrophied Lips Among Lake Malawi Cichlid FishesGenome Biol Evol 14https://doi.org/10.1093/gbe/evac051
- Convergent evolution within an adaptive radiation of cichlid fishesCurr Biol 22:2362–2368
- Gene expression in human keloids is altered from dermal to chondrocytic and osteogenic lineageGenes Cells 10:1081–1091
- jPOSTrepo: an international standard data repository for proteomesNucleic Acids Res 45:D1107–D1111
- A new genus for Melanochromis labrosus, a problematic Lake Malawi cichlid with hypertrophied lips (Teleostei: Cichlidae)Ichthyol Explor Freshwaters 21:209–232
- Regulation of the Versican Promoter by the β-Catenin-T-cell Factor Complex in Vascular Smooth Muscle Cells*J Biol Chem 280:13019–13028
- Understanding explosive diversification through cichlid fish genomicsNat Rev Genet 19:705–717
- East African cichlid fishesEvodevo 14
- NIH Image to ImageJ: 25 years of image analysisNat Methods 9:671–675
- The genetic causes of convergent evolutionNat Rev Genet 14:751–764
- TCC: an R package for comparing tag count data with robust normalization strategiesBMC Bioinformatics 14
- Versican-A Critical Extracellular Matrix Regulator of Immunity and InflammationFront Immunol 11
- An ex vivo model employing keloid-derived cell-seeded collagen sponges for therapy developmentJ Invest Dermatol 133:386–393
- Serum periostin levels are correlated with progressive skin sclerosis in patients with systemic sclerosisBr J Dermatol 168:717–725
Article and author information
Author information
Version history
- Preprint posted:
- Sent for peer review:
- Reviewed Preprint version 1:
Copyright
© 2024, Machii et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
- views
- 106
- download
- 1
- citations
- 0
Views, downloads and citations are aggregated across all versions of this paper published by eLife.