A novel role for lipid droplets in the organismal antibacterial response
- University of California Irvine, United States
- University of Rochester, United States
- Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Spain
- University of Southern California, United States
- Institució Catalana de Recerca i Estudis Avançats, Spain
Figures
Figure 1
LDs kill bacteria via droplet bound histones.
(A). Representative plates in a colony forming assay, showing growth of Gram-negative (Escherichia coli DH5α, top) and Gram-positive (Staphylococcus epidermidis, bottom) bacteria, where a known …
Figure 2
Presence of extranuclear histones depends on the Jabba protein.
(A) Histone H2Av GFP is not detectable in cytoplasmic puncta of Jabbazl01 embryos. Both genotypes show strong signal in nuclei. (B). By immunostaining, endogenous H2A and H2B are absent from the …
Figure 3 with 3 supplements
LD bound histones can kill bacteria in vivo.
(A) Schematic representation of embryo microinjection. Early embryos collected within half an hour of laying were injected with a bacterial suspension, as detailed in ‘Materials and methods’. (B). …
Figure 3—figure supplement 1
2 hr: Additional images of wild-type and Jabba mutant embryos with fluorescent bacteria, 2 hr after bacterial injection.
https://doi.org/10.7554/eLife.00003.006
Figure 3—figure supplement 2
24 hr: Additional images of wild-type and Jabba mutant embryos with fluorescent bacteria, 24 hr after bacterial injection.
https://doi.org/10.7554/eLife.00003.007
Figure 3—figure supplement 3
48 hr: Additional images of wild-type and Jabba mutant embryos with fluorescent bacteria, 48 hr after bacterial injection.
https://doi.org/10.7554/eLife.00003.008
Figure 4
The Jabba protein contributes to improved survival for adult flies.
(A and B) Adult Drosophila lacking Jabba (A: Jabbaf07560; B: Jabbazl01) have reduced survival when challenged by bacteria. Wild-type and Jabba mutant (Jabbazl0 and Jabbaf07560) adult flies were …
Figure 5
Bacterial cell wall components release droplet bounds histones in a dose dependent manner.
(A). Increasing concentrations of lipopolysaccharide (LPS) in the buffer releases droplet bound histones from purified LDs. (B). Lipoteichoic acid (LTA) causes the dose dependent release of histones …
Figure 6
Histones are on mammalian LDs and respond to LPS.
(A). Western blot analysis of LDs (LD) purified from hepatocytes of mice injected with (+) or without (−) LPS. Antibodies against ALDI, histones H2A, H2B and H3 were used. Whole liver homogenate (h) …
