GLV production and emission in transformed lines; graphs show means+SEM. (A) GLVs extracted with hexane from leaf tissue of field-grown WT, irPI, and hemi-irLOX2 plants grouped in triplets for infestation M4 in 2011(Figure 4A). Leaves were harvested from every plant at the beginning (June 14) and in the middle of M4 (June 22) and leaves from plants in 10 randomly chosen triplets were analyzed. Only (E)-hex-2-enal and (Z)-hex-3-en-1-ol were quantifiable in leaf extracts. Different letters (a and b) indicate significant differences (p≤0.05) in Scheffe post hoc tests following one-way ANOVAs for (Z)-hex-3-en-1-ol (top panel, F2,26=9.556, p=0.001; bottom panel, F2,26=12.196, p<0.001). For raw data, see F4A_SchumanBarthelBaldwin2012GLVpools.xlsx (Dryad: Schuman et al., 2012). (B) GLVs measured in headspace samples of leaves from field-grown (top panel, N=3) or glasshouse-grown plants (bottom panel, N=4). For field-grown plants, leaves were harvested and measured on May 21 (just before M3). Intact leaves were kept fresh by placing petioles in water. Immediately before each measurement, one leaf was treated with wounding and M. sexta oral secretions (W+OS); a 1-cm2 disc was stamped out and placed in a 4-mL GC vial. After 15 min the headspace in the vial was measured with a Z-Nose 4200 and total alcohols and aldehydes were quantified. Different letters (a and b) indicate significant differences (p<0.05) in Scheffe post hoc tests following one-way ANOVA (F3,8=7.346, p=0.011). For glasshouse-grown plants, leaves were left on plants, treated with W+OS, and enclosed in padded, 50 mL food-quality plastic containers for 3 hr while the headspace was pulled over a Poropak Q filter. Filter eluents were measured by GC-MS. Three-hour headspace samples contained (Z)-hex-3-en-1-ol, (E)-hex-2-en-1-ol (forms from (E)-hex-2-enal on filters over trapping periods longer than 20 min), (Z)-hex-3-enyl acetate, (Z)-hex-3-enyl butanoate, (Z)-hex-3-enyl isobutyrate, and (Z)-hex-3-enyl propanoate, all of which showed the pattern shown for the total amount. For raw data, see F4B_SchumanBarthelBaldwin2012GLVheadspace.xlsx (Dryad: Schuman et al., 2012). (C) Transcripts of LOX2 in unelicited leaf tissue (control), and at the point of maximum accumulation in W+OS-elicited leaf tissue in glasshouse-grown plants (N=5). For raw data, see F4C_SchumanBarthelBaldwin2012LOX2transcripts.xlsx (Dryad: Schuman et al., 2012). a, b Different letters indicate significant differences between genotypes (p<0.001) in Scheffe post hoc tests following a two-way ANOVA on log2-transformed data with factors treatment and genotype (genotype F3,32=635.477, p<0.001, treatment F1,32=0.021, p=0.887). L.O.D.: below limit of detection for measurement.