Doxorubicin blocks proliferation of cancer cells through proteolytic activation of CREB3L1
- University of Texas Southwestern Medical Center, United States
Figures
Figure 1
Doxorubicin stimulates RIP of CREB3L1.
(A) Schematic diagram of CREB3L1. (B),(D) On Day 0, Huh7 cells (B) or wild type and mutant CHO cells (D) were seeded at 4 × 105 cells per 60 mm dish. On day 1, cells were treated with 500 nM …
Figure 2
Doxorubicin induces transcription of genes activated by CREB3L1.
(A),(B) On day 0, indicated cells were seeded at 3 × 105 cells per 60 mm dish. On day 1, the cells were treated with the indicated concentration of doxorubicin. On day 2, 24 hr after the treatment, …
Figure 3
CREB3L1 is required for doxorubicin to suppress proliferation of Huh7 cells.
(A) On day 0, indicated cells were seeded at 1.5 × 105 cells per 60 mm dish. On day 1, they were treated with the indicated concentrations of doxorubicin. On day 3, 48 hr after the treatment, the …
Figure 4
Sensitivity of cancer cells to doxorubicin is correlated to their expression of CREB3L1.
(A),(E) RT-QPCR quantification of CREB3L1 mRNA in indicated cells with its value in Huh7 (A) or MCF-7 cells (E) set to 1. (B),(F) Effect of doxorubicin on proliferation of the indicated cells was …
Figure 5
CREB3L1 activation is independent from DNA breaks.
(A) On day 0, indicated cells were seeded at 4 × 105 cells per 60 mm dish. On day 1, cells were treated with 500 nM doxorubicin or 500 nM etoposide. On day 2, 24 hr after the treatment, the cells …
Figure 6
Doxorubicin stimulates synthesis of ceramide.
(A) On day 0, Huh7 cells were seeded at 2 × 105 per 60-mm dish. On day 1, the cells were treated with or without 500 nM doxorubicin. On day 2, 20 hr after the treatment, the cells were labeled with …
Figure 7
Doxorubicin-induced synthesis of ceramide stimulates cleavage of CREB3L1.
(A),(C) On day 0, Huh7 cells were seeded at 4 × 105 per 60-mm dish. On day 1, the cells were treated with indicated concentrations of myriocin (A) or fumonisin B1 (C) for 2 hr, followed by …
