(A) Domain structure of UNC-116 protein with motor domain in blue, dimerization region in red, and C-terminal regulation domain in orange, length of amino acid is shown. (B) Cell-autonomous rescue of KLP-16::YFP localization. MD, mutant swapping the amino acids ‘KTH' in P-loop of UNC-116 motor domain with ‘AAA'; MT, mutant swapping the MT-BS in UNC-116 tail region with ‘AAAYAA'. n > 50 per group. Error bar indicates standard deviation; ***p<0.0001, χ2 test. (C) and (D) MTs structure highlighted by GFP::TBA-1 in a representative wild-type (C) or unc-116 worm (D). Quantification of GFP::TBA-1 fluorescence density ratio between the dendrite and the ventral axon in wild-type and the unc-116(e2310) animals. (E) Quantification of EBP-2::GFP puncta moving frequency in the dendrite of wild type and the unc-116(e2310) animals. n > 20 per group. Error bar indicates standard deviation; **p<0.001, ***p<0.0001, Student's t-test. (F) A schematic model showing kinesin-1 cross-linking and sliding MTs out of the dendrite.