(A) Schematic drawing of the clonal induction with hsFlp/FRT-mediated mitotic recombination. After egg laying, larvae were allowed to grow for 24 hr on yeast paste. After the heat shock, larvae were distributed on different food conditions (e.g., 100 g/l and 10 g/l yeast). The time points of dissection are depicted in green. (B) Third instar eye discs bearing hsFlp/FRT PTEN mutant and control clones (marked by the absence of GFP) of larvae reared under varying yeast concentrations. (C) Eye discs with hsFlp/FRT PTEN mutant and control clones (marked by the absence of GFP) induced at the same time and conditions, reared on 10 g/l yeast food, and dissected at the indicated age of the clones. (D) Eyes bearing eyFlp/FRT PTEN mutant and control clones (marked by the absence of pigmentation) of animals reared on 100 g/l and 10 g/l yeast food, respectively. (D′) Quantification of the respective eye sizes. (E) Scanning electron micrographs of eyes almost exclusively composed of PTEN mutant or control tissue of animals reared on 100 g/l and 10 g/l food, respectively, and the quantification of ommatidia size (E′) and number (E′′) from PTEN mutant and control eyes.