The insulin receptor cellular IRES confers resistance to eIF4A inhibition
- Brandeis University, United States
Figures
Figure 1
Simplified insulin/insulin-like growth factor signaling diagram.
(A) When Insulin receptor or Insulin-like growth factor receptor is active signaling through AKT inhibits Foxo transcription factors and activates mTOR. mTOR in turn inhibits 4E-BP and activates …
Figure 2
Foxo activates Pdcd4 in Drosophila cells.
(A) Reanalysis of ChIP-chip data from Teleman et al. (2008). Genomic Browser view of Foxo binding to the Pdcd4 locus in starved larva. The data are plotted as the enrichment (log2) over mock …
Figure 3
Drosophila insulin receptor 5′UTR provides resistance to Pdcd4.
(A) Diagram of dicistronic reporters. Translation of the Firefly ORF requires internal ribosome entry. Firefly to renilla activity ratio provides an indication of IRES activity. (B) The Drosophila …
Figure 4
Drosophila insulin receptor 5′UTR provides resistance to eIF4a inhibition.
(A) Top: Diagram of RNAs used in the in vitro translation assays. Bottom: Titration of hippuristanol in in vitro translation assays. The shade of the bars indicates the final concentration of …
Figure 5
Mammalian insulin receptor and insulin-like growth factor receptor 5′UTR provide resistance to eIF4a inhibition.
(A) Diagram of RNAs used in the in vitro translation assays. (B) In vitro Translation in the absence (white bars) and presence (black bars) of excess m7G analogue. Data are plotted as the fraction …
Additional files
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Supplementary file 1
Oligonucleotides used in this study.
All primers written 5′ to 3′.
- https://doi.org/10.7554/eLife.00542.008
