(A) MS/MS fragmentation spectra of peptides from TbCLK1/2, TbGSK3short, and β-tubulin after labeling with 2, affinity purification, and trypsinization. Red text: residues bearing an iTRAQ tag; ‘m’: oxidized Met. iTRAQ reporter ions for each peptide are shown to the right (m/Z 114, 115, 116, and 117 correspond to lysate samples pretreated with 0, 20, 200, or 1000 nM hypothemycin, respectively). (B) Normalized recovery values, based on iTRAQ quantification, for all identified protein kinases and 25 non-kinases (blue lines) with ≥3 unique peptides. (C) Recovery of protein kinases as a function of hypothemycin pretreatment. Values for each hypothemycin pretreatment condition are derived from mean iTRAQ reporter ion counts normalized to vehicle across all peptides identified (± SD). Asterisks indicate values derived from a single spectrum.