(A) Schematic representation of the mevalonate pathway and its different end products. The steps catalyzed by HMG-CoA reductase and the squalene monooxygenase Erg1 are indicated. Adapted from Goldste…
(A) Abundance of the indicated Erg pathway components in endoplasmic reticulum-associated protein degradation (ERAD) mutants relative to wt cells, as detected by mass spectrometry upon SILAC …
(A) Expression of ERG1 or ERG1-derivatives with the indicated lysine mutations from a plasmid rescues the growth of yeast cells upon repression of the endogenous ERG1. A yeast strain expressing …
(A) The degradation of Erg1 or the indicated Erg1 lysine mutants expressed from the constitutive glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter was followed after inhibition of protein …
(A) Schematic representation of the ergosterol biosynthetic pathway highlighting the enzymatic steps affected by the small inhibitor zaragozic acid, Ro48-807, and fluconazole. The step catalyzed by …
(A) The degradation of the Doa10 substrates Vma12-Ndc10902–956-HA and Erg1 was followed after inhibition of protein synthesis by cycloheximide in wt control cells (DMSO) or in wt cells treated for 2 …
(A) Relative amounts of ergosterol, lanosterol, and ergostadienol in cells with the indicated genotype. Cells were grown in synthetic complete (SC) media until early stationary phase, and lipids …
(A) The degradation of endogenous squalene monooxygenase (SM) was followed after inhibition of protein synthesis by cycloheximide in sterol-deprived HEK293 cells treated with control siRNA or siRNA …
The degradation of endogenous squalene monooxygenase (SM) was followed after inhibition of protein synthesis by cycloheximide in sterol-deprived HEK293 cells transfected with Teb4-myc or …
(A) Schematic representation of the feedback inhibition systems required for sterol homeostasis in yeast (left) and mammals (right) previously characterized (dotted lines) and described here (solid …
(A) Yeast strains used in this study. (B) Plasmids used in this study. (C) Primers used in this study.