(A) Total cell counts and CD4 T cell counts in mLNs of mice on day 12 post infection after treatment with DT or PBS on days 7, 9 and 11. CCR2-DTR mice received naive ESAT-6-specific C7 T cells the day before infection and were treated with DT or PBS on days 7, 9 and 11, mLNs (B) were harvested on day 12, lungs (C) were harvested on day 21 and ESAT-6-specific C7 T cells were visualized. (D) Cumulative data from experiments shown in (B) and (C) showing the total number of ESAT-6-specific C7 T cells in mLNs and lungs. (E) CFU plots of lungs of mice that received naive EAST-6-specific C7 T cells and were treated with DT or PBS, as indicated, and were harvested on day 21 post infection. Each dot represents an individual mouse. Five mice per group are included in the bar graphs shown in (A). Error bars denote SEM. Data are representative of three independent experiments.