(A) Morphology of the juvenile light organ, highlighting the external ciliated epithelium (CE; false-colored blue) in a scanning electron micrograph (left), and the internal features with which the …
Wild-type, motB1 and flrA strains were grown to mid-log phase and inoculated into SWT medium supplemented with 0.3% agar. Plates were visualized after incubating for 11 hr at 28°C.
The motB1 mutant was grown to mid-log phase in SWT broth, and visualized by transmission electron microscopy (TEM) after negative-staining. Scale bar indicates 1 µm.
The flrA mutant was grown to mid-log phase in SWT broth, and visualized by negative-stain TEM. Scale bar indicates 1 µm.
As described in the ‘Materials and methods’, squid were exposed for 10 hr to purified V. fischeri lipid A, then stained with acridine orange. White arrowheads indicate two of the AO-positive nuclei …
As described in the ‘Materials and methods’, squid were exposed for 10 hr to either no V. fischeri (apo), wild-type V. fischeri, or purified V. fischeri lipid A alone. Early-stage apoptosis was …
(A) Negative-stain TEM of a wild-type V. fischeri cell. Dashed box, shown larger within the solid box, highlights the distal tip of two flagella, one of which displays dissociation of the sheath …
Indicated strains were inoculated into SWT and grown at 28°C under microaerobic conditions.
(A) Silver-stained gel image of purified V. fischeri LPS separated by SDS-PAGE used to generate a standard curve by densitometry. (B) Total dry weight following dialysis of 50 ml of cell-free …
Levels of reactogenic LPS in mid-log culture supernatants (OD600 ≈ 0.5) of indicated strains, as measured using the LAL assay. (*), p<0.05, and (**), p<0.01, as analyzed by one-way repeated measures …
Indicated strains were grown to mid-log phase and inoculated into SWT medium containing 0.3% agar. Plates were visualized after incubation for 11 hr at 28°C.
Levels of reactogenic LPS, as measured using the LAL assay, in cell-free supernatants of mid-log cultures (OD600 ≈ 0.5) of V. fischeri wild-type and motX strains. (**), p<0.01 as determined by …
Wild-type and motX strains were grown to mid-log phase and inoculated into SWT medium containing 0.3% agar. Plates were visualized after incubating for 8 hr at 28°C.
The motX mutant was grown to mid-log phase in SWT broth, and visualized by negative-stain TEM, revealing normal sheathed flagella. Scale bar indicates 1 µm.
(A) Reactogenic LPS released into mid-log (OD600 ≈ 0.5) culture supernatants by wild-type V. cholerae O395N1 and its motAB (i.e., pomAB) derivative, grown in SWT at 37°C, was measured by the LAL …