(A and B) Electron microscopy of wild type and AIM24 deletion mutant. Cells were grown in either YPD or YPG and fixed with glutaraldehyde, contrasted with osmium tetroxide and uranylacetate. Ultrathin sections were obtained and contrasted with uranylacetate and lead citrate. (A) Wild type. a–c, cells grown on YPD; d–f cells grown on YPG; a and d, cells (scale bar, 1 µm); b, c, e, f, mitochondrial profiles (scale bar, 0.2 µm). (B) Δaim24. a–i, cells grown on YPG; j–o cells grown on YPD; a, b, j and k, cells (scale bar, 1 µm). c–i and l–o, mitochondrial profiles (scale bars, 0.2 µm). (C) Fluorescence microscopy of wild type and Δaim24 cells expressing mitoGFP. Cells were grown at 30°C on YPD or YPG medium to stationary phase (scale bar, 5 µm).