(A) Schematic representation of the NiTrac1 sensor construct. Aphrodite, yellow; mCerulean, light blue; CHL1/NRT1.1/NPF6.3 dark blue; TM, transmembrane domain. (B) Emission spectra for NiTrac1 … see more
(A) Schematic representation of the PepTrac sensor constructs. AtPTR1, 2, 4, and 5 were used for PepTrac sensor creation. Three-dimensional model of AFP-PTRs-mCerulean chimeric protein based on the … see more
(A) Biphasic nitrate uptake kinetics of the fluorescence response of NiTrac1 (red) and biphasic nitrate uptake transport kinetics of CHL1/NRT1.1 (Black). (B) Monophasic nitrate uptake kinetics of … see more
(A) Three-dimensional model of CHL1 protein based on the crystal structures of bacteria (‘Materials and methods’). Red square, potential substrate binding pocket. Right panel, enlarged potential … see more
Oocytes were voltage clamped at −40 mV and stepped into a test voltage between −20 and −180 mV for 300 ms, in −20-mV increments. The currents (I) shown here are the difference between the currents … see more
The injected oocytes with various cRNA of CHL1 mutants were incubated with 0.25 mM and 10 mM K15NO3 buffer at pH 5.5 for about 1.5∼2 hr, and their 15N content was determined as described in … see more
CHL1 column: wild type and various mutants of CHL1. 15N column: nitrate uptake measured in oocytes using 15NO3− (checkmark ✓ indicates transport activity detected, whereas × indicates no … see more
Known interactors or regulators, such as CIPK8, CIPK23, CBL1, and CBL9 as well as other interactors identified in a large-scale membrane protein interaction screen were co-expressed with NiTrac1 in … see more